Objective To establish a recombinant retroviral vector containing insulin-like growth factor-1(IGF-1) gene and to provide the basis for the application of IGF-1 in treating nervous system diseases such as stroke.Methods The plasmid pcDNA3.1-IGF-1 was cut by EcoR I/Xho I,and subcloned to retroviral vector pLXSN,resulting in the recombinant plasmid(pLXSN-IGF-1.)The recombinant IGF-1 expression vector was evaluated by using enzyme cutting and sequencing.By the Lipofectamine 2000,pLXSN-IGF-1 was transferred to packaging cell line-pA317.Culture supernatant of these cells was detected for titration of the recombinant virus.Results The two fragments from recombined IGF-1 eukaryotic expression vector by EcoR I and Xho I represented 400?bp and 6.0?kb by agarose electrophoresis,and PCR showed positive fragment which was about 400?bp long,and sequence analysis showed the same sequence as expected.The cell line pA317-IGF-1 was established,and average virus titer of the recombinant virus in the culture supernatant was about 6.5?10~5 CFU/ml.Conclusion A recombinant retroviral containing IGF-1 gene was successfully constructed.

Breast Neoplasms ; diagnostic imaging ; radiotherapy ; Female ; Humans ; Imaging, Three-Dimensional ; Mastectomy, Segmental ; Postoperative Period ; Radiotherapy Planning, Computer-Assisted ; methods ; Respiration ; Surgical Instruments ; Tomography, X-Ray Computed ; methods ; Tumor Burden

Adult ; Breast Neoplasms ; diagnostic imaging ; radiotherapy ; surgery ; Cone-Beam Computed Tomography ; Female ; Humans ; Mastectomy, Segmental ; Postoperative Period ; Radiotherapy Planning, Computer-Assisted ; methods ; Respiration

Background and purpose: Peritoneal metastasis of gastric cancer is mainly discovered in the ad-vanced cancer. Nonetheless, the clinical applicability of each tumor biomarker in peritoneal metastasis of gastric cancer is still ambiguous. Therefore, this study investigated the diagnostic value and clinical significance of CEA, CA125 and CA72-4 in gastric carcinoma patients with peritoneal metastases. Methods: A total of 108 gastric carcinoma patients with peritoneal metastases from Jan. 2008 to Dec. 2013 were studied. All patients were diagnosed by imaging, operations and pathological examination, and also received intravenous or intraperitoneal chemotherapy. Serum tumor markers such as CEA, CA125 and CA72-4 were determined during diagnosis and before each chemotherapy. The diagnostic sensitivity of single marker and combined detection with 2 or 3 markers were analyzed. The correlations among the serum tumor markers and clinical pathological factors, chemotherapeutic effects and survival time were analyzed. Results: Positive rates of CEA, CA125 and CA72-4 were 20.4%, 46.3% and 45.4% in gastric cancer patients with peritoneal metastases, respectively. For these patients, the positive rates of CEA/CA125, CEA/CA72-4, CA125/CA72-4 and CEA/CA125/CA72-4 were 54.7%, 52.8%, 69.5% and 79.6%, respectively. The combined detection of 3 tumor markers was much better than single marker detection (P<0.05). Positive rates of CEA, CA125 and CA72-4 were correlated with the ECOG scale (P<0.05). Positive rate of CA125 was associated with ascites (P<0.001), while positive rate of CA72-4 was associated with ovarian metastasis (P<0.05). Median survival time of patients with positive rates of CEA, CA125 and CA72-4 was significantly lower than that of the patients with normal levels of these markers (P<0.05). Compared with pre-treatment, the levels of all three tumor markers significantly declined after three cycles of chemo-therapy (P<0.05). The decline in CA125 level after chemotherapy was significantly correlated with decreased amount of ascites (P<0.05). The tumor markers turned negative after 3 cycles chemotherapy in patients with positive markers upon initial diagnosis, their survival was significantly prolonged (P<0.001). Conclusion: Combined detection of serum CEA, CA125 and CA72-4 can significantly promote diagnostic rate of gastric cancer with peritoneal metastasis, and may be helpful in evaluating chemotherapeutic effects and predicting prognosis.

OBJECTIVE: To study the basic characteristics of three bioactive fungi, the interrelationship of three fungi and the relationship between three fungi and their host, thus to provide data for the theoretical research and the application practice. METHODS: The colonial morphology and microscopic characteristics were studied by light microscopy, and the relationships were studied by agar diffusion method. RESULTS: The basic characteristics of three bioactive fungi were obtained, and the relationships under in vitro conditions were clarified. CONCLUSION: The results of this study will be of great value to the studies and use of the fungi, and it will provide important data for research on the mechanism of fungi inducing Aquilaria sinensis to produce Agilawood.

OBJECTIVE: To develop an enantioselective HPLC-MS-MS method for the pharmacokinetic study of flurbiprofen transdermal patches. METHODS: The analyte and internal standard, ketoprofen, were extracted from plasma by methyl tert-butyl ether, analyzed on a Chiralpak AD-RH column(4.0 mm×150 mm, 5 μm)with mobile phase consisting of acetonitrile-ammonium acetate buffer solution(2 mmol·L-1 pH 5.3, 50:50, V/V), and detected with UV detector at 273 nm. RESULTS: The assay was linear over the concentration range of 2.0-1000 ng·mL-1 and the intra- and inter-day precision over this range were not more than 5.8%. The limit of quantification in plasma was 2.0 ng·mL-1. CONCLUSION: The method established here is accurate, precise, rapid, sensitive, and successfully applied to the enantioselective determination of flurbiprofen in the pharmacokinetic study of flurbiprofen transdermal patches in rabbits.

OBJECTIVE: To investigate the feasibility of utilizing three fungi YNAS04, YNAS06 and YNAS08 to induce the formation of agilawood from Aquilaria sinensis in field and laboratory, respectively. METHODS: Agarwood was obtained by inoculating the fungus in the stem of A. sinensis under natural conditions, and the main ingredients of the volatile oil from induced agilawood were analyzed by GC-MS. The authenticity of the used fungi was detected by the method of morphology and ribosomal internal transcribed spacer. RESULTS: The weights of the induced materials from YNAS04, YNAS06 and YNAS08 were (1.786±0.473), (2964±0.492) and (3.615±0.591) g after six months, which were heavier than blank control (1.325±0.407) g and negative control (1.462±0.417) g, respectively. The fungus inoculated in the stem of A. sinensis and became the dominant fungi in the tissue under natural conditions. Sesquterpenoids and chromone analogues were detected from three fungi inducing materials of six months old. CONCLUSION: Utilizing fungi to induce the formation of agilawood from Aquilaria sinensis has the advantages of spending less time and producing high-quality secondary metabolites. This method has important application foreground in large-scale agilawood production.

OBJECTIVE: To optimize the solid culture condition of three fungi which can induce Aquilaria sinensis to produce agilawood, thus to provide data for the production and application of the fungi. METHODS: Single factor experiment method was applied in the study. RESULTS: The optimal solid culture medium formula, temperature, pH value, moisture, the density of materials and illumination conditions for three fungi were obtained. CONCLUSION: The optimized method can provide high quality fungi for prompting Aquilaria sinensis to produce agilawood at larger scales. Copyright 2012 by the Chinese Pharmaceutical Association.

OBJECTIVE: To screen the fungi which can induce Aquilaria sinensis to produce active substance. METHODS: Fungus was inoculated in the stem of A. sinensis under natural conditions. The penetration and planting of fungus in stem of A. sinensis as well as the interaction of the two organisms were studied by microscope and scanning electron microscopy, and the main ingredients of the volatile oil in induced agilawood were analysed by GC-MS. RESULTS: Three active strains inducing the formation of agilawood were obtained, which was reported for the first time in the world. The main ingredients of the volatile oil were similar between the induced agilawood and the natural materials. CONCLUSION: It is very important for us to use the fungus in the induction of agilawood and in the sustainable utilization of this special medicinal material. Copyright 2012 by the Chinese Pharmaceutical Association.

The Halliwick concept is a kind of aquatic kinesitherapy concept to reduce disability and improve quality of life through activities in water such as swimming. It mainly includes the Halliwick Ten-Point-Programme aimming at teaching siwmming and the Water Specific Therapy focusing on impairments of body functions or body structure. The Halliwick concept can be relatead to the International Classification of Functioning, Disability and Health very well, and its therapeutic effects has been supported by more and more evidence-based-studies.