Continuous renal replacement therapy( CRRT) employs convection and adsorption to re-move pro-and anti-inflammatory mediators from plasma, helping to restore the immunologic homeostasis. CRRT also provide uninterrupted clearance of retained endogenous and exogenous toxins, along with acid-base,electrolyte,hemodynamics and volume homeostasis.CRRT may improve outcomes in critically ill pa-tients with sepsis-induced acute kidney injury,septic shock and multiple organ dysfunction syndrome.Region-al citrate anticoagulation, as compared to unfractionated heparin, report better filter survival times and less bleeding.Antibiotic dosing decisions should be individualized to take into account patient-related,CRRT-relat-ed,and drug-related factors.

Objective To observe the effect of bone marrow mononuclear cells (BMMNCs) transplantation on high mobility group box1 (HMGB1) migration in stroke mouse. Methods BMMNCs were harvest and injected intravenously into stroke mouse model; modified neurological severity scores(mNSS) were tested; brain infarct vol-ume and related protein expression levels were measured, then HMGB1 migration were observed. Results mNSS score and brain infarct volume in transplantation group weresignificantly lower than vehicle group , while HMGB1 expression were significantly higher than vehicle group. The expression levels of RAGE and TNF-α in transplanta-tion group were significantly lower than vehicle group. Immunofluorescence assay showed that the release of HMGB1 in brain penumbra area of transplanted mouse was significantly less than vehicle groups. Conclusions BMMNCs transplantation could inhibit the release of HMGB1 in mouse brain.

Objective To evaluate the prognostic value of combing pre-procedural red cell distribution width(RDW)with serum uric acid(SUA)levels in patients with acute coronary syndrome(ACS)undergoing percutaneous coronary intervention(PCI).Meth-ods A total of 1 52 consecutive patients with ACS who underwent successful PCI within 12 hours after onset of symptom were en-rolled.RDW and SUA were determined within 12 hours before PCI.The patients were divided into 3 groups according to the cut-off values showed by receiver operating characteristic(ROC)curve:73 cases in group I,RDW<14.5% and SUA<402 μmol/L;50 cases in group Ⅱ,RDW< 14.5% and SUA≥ 402 μmol/L,RDW≥ 14.5% and SUA< 402 μmol/L;29 patients in group Ⅲ,RDW>14.5% and SUA>402 μmol/L.The pre-procedural RDW and SUA status associated with 30 days any cause mortality and major adverse combined cardiac events including revascularization,non-fatal recurrent myocardial infarction,secondary heart failure,rehos-pitalization and death were analyzed.Results Pre-procedural RDW and SUA level predicted 30 days cardiac mortality,RDW and SUA level correlated linearly(r=0.336,P =0.001).30 days major adverse combined cardiac events and any cause mortality were significantly different among the three groups(P =0.031,P =0.012).Conclusion Pre-procedural RDW≥ 14.5% and SUA≥402μmol/L indicates poor prognosis in ACS patients underwent successful PCI.Therefore the combination of RDW and SUA measure-ment should be taken into consideration for risk stratification to decide about the management strategies in ACS patients.

Objective To explore the changes of the levels of CA-125 antigen(CA125) and vascular endothelial growth factor (VEGF) in serum and peritoneal fluid in patients with endometriosis (EMT), and the relationship with EMT. Methods One hundred and twenty patients with EMT (EMT group)and 98 patients with uterine fibroids (control group) were enrolled in this study. The levels of CA125 and VEGF in serum and peritoneal fluid were detected by enzyme linked immunosorbent assay (ELISA), and the results were compared. Results The levels of serum CA125,VEGF in EMT group were significantly higher than those in control group:(40.31±11.14) kU/L vs.(24.71±9.19) kU/L,(59.75±6.87) ng/L vs. (36.38±8.12) ng/L, there were significant differences(P<0.05). The levels of serum CA125,VEGF inⅠ-Ⅱphase andⅢ-Ⅳphase in EMT group had no significant differences ( P>0.05). The levels of peritoneal fluid CA125,VEGF in EMT group were significantly higher than those in control group:(311.46± 107.29) kU/L vs.(158.17± 55.42) kU/L,(73.28 ±10.40) ng/L vs. (40.21 ±9.84) ng/L, there were significant differences (P<0.05). The levels of peritoneal fluid CA125,VEGF in Ⅲ-Ⅳ phase were significantly higher than those in Ⅰ-Ⅱ phase in EMT group:(387.41±70.91) kU/L vs. (308.42±81.21) kU/L, (69.22±7.13) ng/L vs. (55.44±8.23) ng/L, there were significant differences (P<0.05). In EMT group, the levels of CA125 and VEGF in serum were significantly lower those in peritoneal fluid:(40.31±11.14) kU/L vs. (311.46±107.29) kU/L, (59.75±6.87) ng/L vs. (73.28 ±10.40) ng/L, there were significant differences (P<0.05). Conclusions The levels of CA125 and VEGF in serum and peritoneal fluid are closely related to EMT. Peritoneal fluid in monitoring of EMT may be more sensitive and reliable than serum.

By collecting literatures on apoplexy recorded in"Database of Basic TCM disease"from 2001 to 2005,we statistically analyzed changes on volume of documents,major authors,major journals,and contents of study in these literatures.

Objective: To construct the Behavior Inhibition Scale. Methods: The compilation of items was in accordance with the content validity of seven kinds behavior inhibition. The Self Consistency and Congruence Scale and SCL-90 were also applied as validity index. Results: Factor analysis on 660 subjects rating on original 86 items of Behavior Inhibition Scale yielded seven factors composed of 42 items, measuring the inhibition of help-seeking, self-confidence, independence, negativeness, alienation, dominate, and refuse, respectively. Behavior inhibition correlated significantly with SCL-90 and self congruence, and significant sex and education differences were also detected. Conclusion: The Behavior Inhibition Scale has good reliabilities and well validated.

Objective: To investigate the characteristics of Behavior Styles personality dimension among subjects of dif- ferent age, genders, marriage status, and occupations. Methods: After subjects fulfilled Chinese Personality Scale (QZPS), score differences of Behavior Styles and its sub- factors were compared among age, genders, marriage status, and occupa- tions. Results: Significant differences were detected among different age, genders, and occupations. Males have lower scores than females; the elder the subjects, the higher the scores; executives and technicians have the same scores, and both have higher scores than workers and peasants. Some interactions were also significant. Conclusion: There were sig- nificant differences among subjects of different gender, age, and occupation.

OBJECTIVE: To study extraction method of anthraquinones components of Radix et Rhizoma Rhei and optimize extraction technology.METHODS: Ultrasonic extraction,heating reflux extraction and soxhlet extraction were adopted to extract Radix et Rhizoma Rhei respectively.3 kinds of extraction technology were compared with the content of total anthraquinone,aloe-emodin,rhein,emodin,chrysophanol,physcione as index.The extraction technologies of UV and 5 kinds of anthraquinone compounds were optimized by uniform design method on the basis of previous study.RESULTS: Heating reflux extraction which was optimal extraction technology was as follows: extracting time of 90 min,extracting for 1 time,methanol concentrations of 95%,medicinal mesh of 2.000 7.Comprehensive score of anthraquinones could reach 6.556.CONCLUSION: The extraction technology is reasonable,available in quality control.

Objective To systematically analyze therapeutic effects of extracorporeal photopheresis (ECP) on refractory steroid-resistant,-dependent or-intolerant graft-versus-host disease (GVHD),to compare remission rates of skin GVHD in detail.Methods Databases were searched for studies concerning remission rates of GVHD after the treatment with ECP,and relevant data were extracted and pooled by using meta-analysis with STATA 12.0 software.Results A total of 22 literatures were reviewed,including 787 patients with refractory GVHD.Meta-analysis showed that the pooled complete remission (CR) rate and overall remission (OR) rate were 57％ (95％ CI:47％-66％) and 79％ (71％-86％) respectively in patients with acute GVHD,27％ (21％-33％) and 63％ (58％-69％) respectively in patients with chronic GVHD,77％ (71％-82％) and 87％ (82％-91％) in patients with acute skin GVHD,as well as 39％ (30％-48％) and 70％ (63％-77％) in patients with chronic skin GVHD.Conclusion ECP is an effective therapy for refractory GVHD,especially for skin GVHD.

Objective To evaluate the safeness of this treatment by comparing the serum IGF-Ⅰ and IGFBP3 concentration level before and after receiving rhGH treatment in pediatric patients.Methods 30 pediatric patients in our hospital receiving rhGH treatment were subjected to monitor serum IGF-Ⅰ and IGFBP3 level before and after the treatment and calculate the IGF-Ⅰ and IGFBP3 ratio.At the same time the serum T3,T4 and TSH are also assayed.Results The average increase of booly heights was(8.77?3.01)cm /y(t=7.773,P0.05),but the serum IGFBP3(t=3.759,P0.05).However,there was statistical significance of the serum IGFBP3 level between subgroups(f=22.964 P0.05).Conclusion The serum IGF-Ⅰ and IGFBP3 level are able to be used as one of clinical evaluation benchmarks to detect the risks of inducing tumor during the rhGH treatment in the pediatric patients.