Objective To explore the medicinal values of the buds of Lonicera ruprechtiana Regel.by analyzing its chemical constituents. Methods The buds of Lonicera ruprechtiana Regel.were extracted with 70% ethanol,the extractives were purified with macroporous resin and isolated with silica gel and ODS column chromatography.NMR,UV,IR Spectrometers were used to obtain spectroscopic data.Results According to the single spot in TLC,five compounds were isolated from the buds of Lonicera ruprechtiana Regel.,and their structures were identifited through physico-chemical constants and spectroscopic analysis.Five compounds were isolated from the buds of Lonicera ruprechtiana Regel.,and they are daucosterol (Ⅰ);7S-O-methylmorroniside(Ⅱ);neohesperidin(Ⅲ);benzyl-O-?-D-xylopyranosyl-(1→6)-?-D-glucopyranoside (Ⅳ);6'-O-?-D-apiofuranosyl sweroside (Ⅴ).Conclusion All these compounds are isolated from the buds of Lonicera ruprechtiana Regel.for the first time.

Objective To understand the status quo of continuing medical education in different levels of hospitals in primary health technical personnel,and provide suggestions for the development of continuing medical education.Methods Questionnaire survey,Statistical analysis and Interview.Results Health technical personne of medical institutions at all levels in primary health technical personnel were generally more emphasis on continuing medical education,but the management system,input and the need are different in the different levels of hospital.Continuing medical education in the basic level hospitals is much lower than the high level hospitals.Conclusions We should strengthen the transformation of continuing education mode,put forward the practical development strategy,improve the level of medical education,and narrow the gap between different levels of hospitals,so as to achieve the purpose of common progress.

Objective To evaluate the safeness of this treatment by comparing the serum IGF-Ⅰ and IGFBP3 concentration level before and after receiving rhGH treatment in pediatric patients.Methods 30 pediatric patients in our hospital receiving rhGH treatment were subjected to monitor serum IGF-Ⅰ and IGFBP3 level before and after the treatment and calculate the IGF-Ⅰ and IGFBP3 ratio.At the same time the serum T3,T4 and TSH are also assayed.Results The average increase of booly heights was(8.77?3.01)cm /y(t=7.773,P0.05),but the serum IGFBP3(t=3.759,P0.05).However,there was statistical significance of the serum IGFBP3 level between subgroups(f=22.964 P0.05).Conclusion The serum IGF-Ⅰ and IGFBP3 level are able to be used as one of clinical evaluation benchmarks to detect the risks of inducing tumor during the rhGH treatment in the pediatric patients.

The key reason for reparative process interruption of femoral head necrosis is the formation of hardening layer, which make it hard for medicine to act on the necrosis center. The research on formation mechanism of hardening layer is limited and the reason for reparative process interruption is unknown. Based on lots of pathological section and literature data, four hypotheses about the reason for reparative process interruption are proposed, including resource competition, damage of stress, disturbance of nerves and stasis of blood vessel. The internal mechanism of incomplete reparation and chronicity of hardening layer was analyze from different aspects. The four hypotheses provides a new thinking for study the reason for reparative process interruption.

Objective: To construct the Behavior Inhibition Scale. Methods: The compilation of items was in accordance with the content validity of seven kinds behavior inhibition. The Self Consistency and Congruence Scale and SCL-90 were also applied as validity index. Results: Factor analysis on 660 subjects rating on original 86 items of Behavior Inhibition Scale yielded seven factors composed of 42 items, measuring the inhibition of help-seeking, self-confidence, independence, negativeness, alienation, dominate, and refuse, respectively. Behavior inhibition correlated significantly with SCL-90 and self congruence, and significant sex and education differences were also detected. Conclusion: The Behavior Inhibition Scale has good reliabilities and well validated.

Objective: To investigate the characteristics of Behavior Styles personality dimension among subjects of dif- ferent age, genders, marriage status, and occupations. Methods: After subjects fulfilled Chinese Personality Scale (QZPS), score differences of Behavior Styles and its sub- factors were compared among age, genders, marriage status, and occupa- tions. Results: Significant differences were detected among different age, genders, and occupations. Males have lower scores than females; the elder the subjects, the higher the scores; executives and technicians have the same scores, and both have higher scores than workers and peasants. Some interactions were also significant. Conclusion: There were sig- nificant differences among subjects of different gender, age, and occupation.

Objective To establish a recombinant retroviral vector containing insulin-like growth factor-1(IGF-1) gene and to provide the basis for the application of IGF-1 in treating nervous system diseases such as stroke.Methods The plasmid pcDNA3.1-IGF-1 was cut by EcoR I/Xho I,and subcloned to retroviral vector pLXSN,resulting in the recombinant plasmid(pLXSN-IGF-1.)The recombinant IGF-1 expression vector was evaluated by using enzyme cutting and sequencing.By the Lipofectamine 2000,pLXSN-IGF-1 was transferred to packaging cell line-pA317.Culture supernatant of these cells was detected for titration of the recombinant virus.Results The two fragments from recombined IGF-1 eukaryotic expression vector by EcoR I and Xho I represented 400?bp and 6.0?kb by agarose electrophoresis,and PCR showed positive fragment which was about 400?bp long,and sequence analysis showed the same sequence as expected.The cell line pA317-IGF-1 was established,and average virus titer of the recombinant virus in the culture supernatant was about 6.5?10~5 CFU/ml.Conclusion A recombinant retroviral containing IGF-1 gene was successfully constructed.

Lack of specific symptoms and signs makes clinical diagnosis of trichinellosis difficult. Epidemiological information is important, such as a history of ingesting raw or undercooked meat. An outbreak can be traced to a group of people dining together. Usual manifestations include abdominal pain or diarrhea with general discomfort in the enteric stage, and fever, eyelid or facial edema, muscle pain in acute stage. Complications, such as myocarditis, pneumonia, encephalitis, may develop in severe cases. Eosinophilia appears between 2 and 5 weeks after infection. Enzyme-linked immunosorbent assay(ELISA) using the excretory-secretory(ES) antigens of the muscle larvae or synthetic tyvelose as antigen is sensitive and specific, the serological method of choice as a screening test. Western blotting is needed to confirm the positive ELISA. Definitive diagnosis depends on the finding of larvae in a muscle biopsy specimen. Albendazole is the drug of choice for its treatment, 20-30 mg/(kg?d), two times daily for 5-7 days. Glucocorticosteroids are given only to severe cases and always be used in combination with albendazole, since they could prolong the intestinal phase of the infection and increase the muscle larval burdens.

Objective To establish a nested-real-time-PCR for the detection of IL-21R mRNA in peripheral blood mononuclear cells(PBMC)of patients with rheumatoid arthritis,and explore its clinical significance.Methods The outer primers of span intron and inner primers were designed for nested-real-time-PCR.To increase specific template,the concentration of primers and enzyme,cycle times were decreased in the first amplification,and the real-time fluorescent quantity assay was performed in the second amplification.The ?-actin gene was used as internal reference,and the results were presented as the ratios of IL-21R mRNA to ?-actin mRNA.Results IL-21R mRNA was detected in 60 patients with RA and 30 healthy controls.The expression of IL-21R mRNA gene in the patients with RA was increased,and the statistical analysis has significant differences(P0.05),but a significant difference was found in the patients of grade III compared with those of grade I and II(P

Objective To identify the clinical and neurophysiological features of multiple system atrophy (MSA) and explore diagnostic values using various techniques in electro neurophysiology Methods Forty four subjects diagnosed as multiple system atrophy (MSA) during 1983 to 2001 in PUMC Hospital were studied retrospectively about their clinical features, routine electromyography (EMG), nerve conductive velocity (NCV), somatosensory evoked potential (SEP), motor evoked potential (MEP), brainstem auditory evoked potential (BAEP) and visual evoked potential (VEP) The clinical features and the electroneurophysiologic results were analyzed in different subgroups (probable MSA and possible MSA, OPCA and non OPCA, classified MSA and unclassified MSA) Results Most patients were found to be suffered from autonomic failure (88 6%) and cerebella dysfunction (88 6%) The pyramidal sign was seen in 72 7% of patients No remarkable statistical differences were found in all of the above three domains The incidence of Parkinsonism was 36 4% of all the patients, which showed significant differences between probable and possible MSA (54 5% vs 18 2%), non OPCA and OPCA (50% vs 7 1%), unclassified MSA and classified MSA (52% vs 15 8%) EMG was abnormal in 7 patients (38 1%), all of them were neurogenic impairments The rates of abnormal EMG were varied differently between probable and possible MSA (54 5% vs 22 2%), non OPCA and OPCA (53 8% vs 14 3%), unclassified MSA and classified MSA (58 3% vs 12 5%), but without statistical differences The abnormal rates in BAEP,SEP, VEP and MEP were 56 7%, 28%, 23 1% and 20 0%, respectively, and there were no significant differences between subgroups Conclusions It should be hard to divide MSA into groups only by the clinical manifestations All EPs were abnormal in some extent The most sensitive test is BAEP in current study, but there were no differences among the subgroups in the rates of abnormal EPs The difference of EMG and NCV between the groups showed that the technique might be helpful in the diagnosis of MSA