OBJECTIVE:To study the genetic diversity of germplasm resources of Duchesnea indica from Hunan. METHODS:The random amplified polymorphic DNA(RAPD)reaction system of germplasm resources was established and RAPD-PCR was ad-opted to detect the expressions of 24 groups of D. indica from Hunan(including 21 wild and 3 cultivated varietos in different geo-graphical distribution). Agarose gel electrophoresis was used to analyze the results and calculate allele number (Na),effective al-lele number (Ne) the polymorphism points percentage (PPB),Nei’s gene diversity index (H) and Shannon’s information index (I). RESULTS:Totally 14 polymorphic primers were screened and 90 electrophoresis bands were amplified,including 81 polymor-phic bands with Na of 1.900 0,Ne of 1.540 1,PPB of 90.0%,H of 0.312 8 and I of 0.467 5. CONCLUSIONS:The genetic diver-sity of D. indica from Hunan is rich,and there were differences in genetic background between the cultivated species and wild spe-cies;clustering results show significant correlation with geographic distribution.

Objective To explore the strategy of constructing a scientific management system for antibiotics in a tertiary hospital,and to provide reference to strengthen the management and rational use of antibiotic.Methods Using PDCA management tools to build a scientifically effective antimicrobial drug management system.Evaluation of management quality of antimicrobial stewardship was constructed by 14 indicators of 5 dimensions.The entropy weight TOPSIS method was used to comprehensively evaluate and analyze the changes in various indicators after the implementation of PDCA improvement.Results Based on the entropy weight TOPSIS analysis,the management quality of antibiotics in 2018 was the lowest with the Ci value 0.313 5,and the management quality of antibiotics in 2022 was the highest with the Ci value 0.747 4.The evaluation of antimicrobial stewardship was divided into five dimensions:for the evaluation of antibiotics use density,the Ci value in 2017 was highest;for the evaluation of antibiotics usage rate,the Ci value in 2021 was the highest;for the evaluation of the submission rate before use of antibiotics,the Ci value in 2022 was the highest;for the evaluation of preventive medication for type I incisions,the Ci value was highest in 2022;the evaluation of the cost dimension of antibiotics for hospitalized patients showed the highest Ci value in 2022.The application of PDCA method for antibiotics management had shown an overall improvement trend.Conclusion Based on PDCA method,collaboration through multiple departments for antibiotics management had achieved significant results.The entropy weight TOPSIS method can be used to evaluate the effectiveness of antibiotics management,to provide reference for the direction of hospital antibiotics control and policy formulation.