1.Assessment of the expression profile of SRY-type high-mobility-group box-9 during chondrogenesis of mice and the effects of transfection of recombinant rat sty-type high-mobility-group box-9 on mice mesenchymal stem cells in vitro
Xin TANG ; Zhongshi XU ; Shuhua YANG ; Yuchen CHEN ; Qi LI ; Congnian YU ; Cao YANG ; Jin LI ; Weihua XU ; Jingyuan DU
Chinese Journal of Rheumatology 2008;12(5):325-329
Objective To examine the gene expression profile of SRY-type high-mobility-group box-9(SOX9) during entochondrostosis of mice and investigate the effects of transfection of the pDC316-SOX-9 on mice mesenchymal stem cells(MSCs) in vitro. Methods cDNA microarray technique with 34 000 genes was used to analyze the gene expression profiles during entochondrostosis in the limbs of mice embryo from E10 to E14. Pathway analysis of SOX9 was performed with GCOS1.2 software. The recombined expression vector pDC316-SOX-9 was constructed and transfected into mice MSCs by lipofectamine. The phenotype changes of cells were observed with cell energometry, HE stain, immunohistochemical method, RT-PCR and ELISA.Results The gene expression of SOX9 during the critical phase of chondrogenesis in mice embryo limbs at E12 was increased evidently. SOX9 might promot chondrogenesis. As compared with vector and blank group,the chondrocytes of the SOX9 transfected group had the tendency of enhanced differentiation. Conclusion SOX9 may promote chondrogenesis. The transfection of SOX9 gene into mice MSCs can promot MSCs differentiate into chondrocyte, which may provide some experimental data for cartilage histoengineering.
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