Objective To investigate the effects of early pregnancy exposure to hazardous indoor air pollutants due to interior decoration and hyperthermia on neurobehavioral development of postnatal rats.Methods Twenty-two pregnant rats were randomly divided into 4 groups.Each group was placed in pollutants cabinet,hyperthermia cabinet,hyperthermia+pollutants cabinet and empty cabinet respectively for 2 h per day for 10 d since their pregnancy.The weight and neurobehavioral development of postnatal rats were observed at different time points.The number of apoptotic and dead neurons in the CA1 region of hippocampus was observed in postnatal rats.The expression of the NR1 subunit of NMDA receptor of hippocampal neurons was also detected.Results Indoor pollutants and hyperthermia could accelerate apoptosis and necrosis of hippocampal neurons in CA1 region of postnatal rats and increase the expression of NR1 subunit of NMDA receptor.Furthermore,hazardous indoor pollutants and hyperthermia could induce remarkable influences on postnatal rats’ neurobehavioral development,learning and memory capability.Indoor pollutants combined with hyperthermia aggravated these effects.Conclusion Exposure to environment of the hazardous indoor pollutants especially combined with hyperthermia during early pregnancy has remarkable influences on the nervous system of postnatal rats.

Objective To observe the effect of early enriched environmental intervention on synaptic ultrastructure and the synaptophysin expression (p38) in hippocampal CA1 area after hypoxia-ischemic brain damage(HIBD) in neonatal rats. Methods The rat model of HIBD was made by the method of Rice in 20 Sprague-Dawley rats, and divided randomly into 2 groups: the intervention group, non-intervention group. Another 10 rats underwent sham-operation as control. From the second day after HIBD, the rats of the intervention group were kept in an enriched environment for 20 d, the rats of non-intervention group and sham-operation group were raised in standard enviroment instead. The hippocampal ultrastructure was observed with electron microscope and the p38 expression was detected immunohistochemically in all rats. Results The ultrastructure of hippocampus CA1 pyramidal neuron in the non-intervention group were distorted as compared with that in the intervention group and control group. The corrected optical densities (COD) of immunoreactive products of the hippocampal p38 were significantly decreased in non-intervention group as compared with intervention group and control group (P0.05). Conclusion Early enriched environment intervention could improve the synaptic reconstruction and reduce the ultrastructural damage induced by hypoxic-ischemia.

Objective To observe the effects of different environment stimulation on the nestin expression in hippocampus and the ability of learning and memory of rats after hypoxic-ischemic brain damage (HIBD). Methods Rat HIBD models were established by the method of Rice in 45 SD rats, then randomly divided into three groups: standard environment stimulation group (SE), enriched environment stimulation group (EE), and impoverished environment stimulation group (IE). Another 15 rats only underwent sham-operation. Different environment intervention that was designed according to Puurunen and Bourgeon’s literatures was applied to the rats on day 2 after HIBD operation. On day 28, Morris water maze was used to evaluate the ability of learning and memory. Then the nestin expression in the hippocampus was measured by immunohistochemistry. Results The ability of learning and memory of IE group reduced and was much lower than that of sham-operation group, SE group and EE group, that of SE group was lower than that of sham-operation group and EE group, but no significant difference between sham-operation group and EE group. Immunohistochemical analysis showed that nestin expression in the hippocampus of EE group significantly increased as compared with that of other groups and that of SE group was stronger than that of sham-operation group and IE group. Conclusion The EE stimulation could increase the nestin expression in the hippocampus of neonatal rats with HIBD, enhance neuranagenesis, and improve the ability of learning and memory, while the IE stimulation could decrease the nestin expression, inhibit neuranagenesis, and impair the ability of learning and memory.

Objective To observe the effect of comprehend intervention on physical and intellectual development of homeless infants.Methods Forty-eight infants aged 8 to 9 months from an orphanage of Chongqing were randomly divided into control group and treatment group.The treatment group received swim,early training and massage every day.All infants were assessed by means of Gesell Development Scale before intervention and one month after intervention.Results Compared with that of the control group,the treatment group's scores significantly improved in all four ergoregion(P

Objective To observe the effects of early enriched environment intervention on the expression of growth-associated protein-43(GAP-43) in the hippocampus of rats with hypoxic-ischemic brain injury (HIBI). Methods After the establishment of HIBI model in SD rats by the method of Rice, the animals were divided randomly into 2 groups: the intervention group and non-intervention group. The sham-operation rats were used as control group. Enriched environment intervention had been administrated to the rats of intervention group since the 2nd day after HIBI. On the 3rd, 7th, 14th, 21st and 28th day, immunohistochemistry and RT-PCR were used to measure the expressions of GAP-43 protein and mRNA in the hippocampus of rats. Results The expression of GAP-43 protein and mRNA in the rat hippocampus were increased in the non-intervention group than in the control group(P

Objective To observe the effects of environmental stimulation at different stages of development on postnatal rats with hypoxia-ischemic brain injury (HIBI). Methods HIBI postnatal SD rats models were established by dissecting and ligating the left common carotid artery, and after 2 h of recovery, exposing to 8% oxygen-92% nitrogen gas mixture for 2 h. The HIBI rats were randomly divided into early intervention groups, late intervention group, non-intervention group. Intervention group were kept in enriched environment before or after weaning for 20 d. Non-intervention group and control group were kept in a standard environment. Space learning and memory were tested with Morris water maze. Results The ability of space learning and memory in early intervention group was better than that in late intervention group (P

Objective To observe the expression of growth-associated protein-43(GAP-43) in the hippocampus of neonatal rats with hypoxic-ischemic brain damage (HIBD). Methods HIBD was established by the method of Rice in 48 SD rats aged 7 days and another 48 matched normal rats served as control. Immunohistochemistry and RT-PCR were respectively used to detect the expression of GAP-43 protein and mRNA in all rat hippocampus on day 8, 10, 14, 21, 28, 35 after HIBD (n=8 at each time point for each group). Results The expression of GAP-43 protein and mRNA in hippocampus was obviously increased in HIBD rats as compared with normal rats, the peak of GAP-43 protein appeared at 3rd week, and the peak of mRNA at 2nd week. Conclusion The expression of GAP-43 protein and mRNA increased in the hippocampus of rats following HIBD, which was possibly related to the recovery of injured hippocampus.

Objective To study the therapeutic effect of combined administration of nerve growth factor(NGF)and ganglioside GM_ 1 on the hypoxic-ischemic brain injury(HIBI)rat model and the relevant involving mechanisms.Methods A total of 120 7-day old rats were randomly divided into 5 groups:sham-operation group,HIBI group,NGF group,GM_ 1 group and NGF+GM_ 1 group.HE staining,immunohistochemical staining and TUNEL staining were employed respectively to study the morphological changes,bcl-2 and Bax expressions and apoptosis in the hippocampal neuron after treatment.Results Pathological changes were observed in the hippocampal neuron after HIBI.In NGF+GM_ 1 group,the level of Bcl-2 expression was increased significantly(P

Objective To construct eukaryotic expression plasmid pEGFP-N1/EPO and to detect its expression in vitro in order to provide experimental evidence for further study on the treatment of hypoxic-ischemic brain damage.Methods The total RNA was extracted from rat kidney.EPO cDNA fragment was obtained by RT-PCR amplication,inserted into pEGFP-N1 vector and identified by restriction endonuclease digestion and nucleotide sequencing.MSCs cells were transfected with the plasmid using Lipofectamine 2000.The expression of EPO protein was detected by immunohistochemistry technique and RT-PCR.Results Confirmed by restriction endonuclease digestion and sequencing,the recombinant plasmid contained the sequence of rat EPO gene was successfully constructed.After transfection with the plasmid,EPO protein could be expressed in MSCs cells.Conclusion The constructed eukaryotic expression vector pEGFP-N1/EPO can express rat EPO in vitro.

Lactose tolerance test with ethanol(LTTE)described by Arola was performed in 41 healthy children and lactose malabsorption(LM)could be diagnosed if blood galactose(B-gal)was less than 0.3 mmol/L or urine galactose less than 2 mmol/L.Then 138 healthy infants and children and 95 children with acute diarrhea were examined for LM or lactose intolerance(LI)with the urine samples of LTTE and symptom-response score(SRS).It was found that LTTE was reliable in the diagnosis of LM ;the incidence of LM or LI increased with the increase of age in children)and RV enteritis was liable to be complicated with LM or Li.