Objective:Egr-1 promoter was amplificated and Egr-PGL plasmid was constructed to study the expression of luciferase in transfected NIH3T3 cells after different doses of X-ray irradiation.Methods:Egr-1p was amlificated by PCR and inserted into PGL3-E vector.The expression of luciferase induced by X-ray was studied by counting the light of luciferase and stubstrate.Results:Egr-1 cDNA was obtained by PCR and was sequenced.The results indicated the sequence was almost correct.The Egr-1p was connected with PGL3 vector and was detected by electrophoresis.The constructs were used to transfect mouse NIH3T3 cells to characterize the regulatory function of Egr-1p after exposure to X-ray irradiation.The results indicate that the expression of luciferase of all groups irradiated is highter than that of 0 Gy group.The expression of groups irradiated is about 5-7.5 times greater than that of 0 Gy group.Conclusion:Egr-1pobtained can induce the expression of its downstream gene after different doses of X-ray irradiation.Low dose irradiation also can do it and it is may be more important in tumor gene therapy.

Objective To study the effect of small heterocyclic compound 8-oxo-3-thiomorpholino-8H-acenaphtho pyrrole-9-carbonitrile(S1) on apoptosis of human androgen independent prostatic carcinoma cells line(PC3) and its mechanism.Methods PC3 cells were cultivated,and divided into different groups: 10.00,5.00,1.00,0.50,0.10,0.05 and 0.01 ?mol?L-1 S1 groups,meanwhile,PC3 control group and cyclophosphamide group were set up.MTT was used to detect the inhibitory rate of PC3 cell proliferation.Flow cytometry was used to detect the inducing effect of S1 on apoptosis of PC3 cells.Caspase 3,8,9 kits were used to detect apoptosis route.Results The inhibitory rates of PC3 cells induced by 0.10-10.00 ?mol?L-1 S1 were significantly higher than that in cyclophosphamide group(P

0.05).The apoptotic percentage was increased significantly after X-rays irradiation with the dose of 6 Gy(P