Objective:Egr-1 promoter was amplificated and Egr-PGL plasmid was constructed to study the expression of luciferase in transfected NIH3T3 cells after different doses of X-ray irradiation.Methods:Egr-1p was amlificated by PCR and inserted into PGL3-E vector.The expression of luciferase induced by X-ray was studied by counting the light of luciferase and stubstrate.Results:Egr-1 cDNA was obtained by PCR and was sequenced.The results indicated the sequence was almost correct.The Egr-1p was connected with PGL3 vector and was detected by electrophoresis.The constructs were used to transfect mouse NIH3T3 cells to characterize the regulatory function of Egr-1p after exposure to X-ray irradiation.The results indicate that the expression of luciferase of all groups irradiated is highter than that of 0 Gy group.The expression of groups irradiated is about 5-7.5 times greater than that of 0 Gy group.Conclusion:Egr-1pobtained can induce the expression of its downstream gene after different doses of X-ray irradiation.Low dose irradiation also can do it and it is may be more important in tumor gene therapy.

Objective To explore the relationship between family environment and parental rearing patterns and adolescent schizophrenia , for the prevention of adolescent mental and provide scientific basis of schizophrenia . Methods In adolescent patients with schizophrenia in 101 cases( case group ) ,101 cases of the other without a histo-ry of psychosis patients as control group ,using the family environment scale ,EMBU for two groups of patients quality of life evaluation,non conditional Logistic regression analysis of related factors were analyzed .Results Intimacy, emotion expression,entertainment and father emotional warmth and understanding (F1) was a protective factor(OR=0.643,95%CI=0.235~0.863,P=0.01;OR=0.582,95%CI=0.168~0.906,P=0.01;OR=0.478,95%CI=0.174~0.834,P=0.01),but the contradiction,the father punished severely(F2),father′s refusal and denial(F5) and mother′s over interference(M2) as a risk factor(OR=1.535,95%CI=1.096~2.187,P=0.01;OR=1.516, 95%CI=1.097~2.096,P<0.01;OR=1.375,95%CI=1.034~1.989,P=0.01;OR=1.347,95%CI=1.272~1.915,P<0.01).Conclusion Good family environment and parental rearing pattern can reduce the incidence of schizophrenia .

Objective To investigate the expressions of proliferating cell nuclear antigen (PCNA), bcl-2, and c-myc protein, and to explore diagnostic value of DNA ploidy in benign and malignant thyroid neoplasms. Methods The expressions of PCNA, bcl-2 and c-myc proteins in 36 cases of thyroid adenomas and 37 cases of thyroid carcinomas were examined by immunohistochemistry technique. DNA ploidy was measured by imaging analysis technique in 8 cases of thyroid adenomas and 17 cases of thyroid carcinomas. Results Among thyroid carcinomas, the positive rates of PCNA (43.02?31.16)% and c-myc protein 89.2% were significantly higher than those of thyroid adenomas (16.15?9.28)% and 50.0% respectively (both P