Objective To investigate the sequence variations of mitochondrial D-loop region in chronic glomerular nephritis patients and family members and their possible associations with chronic glomerular nephritis.Methods 20 patients with chronic glomerular nephritis and 48 unaffected pedigree members,as well as 122 cases of normal control were recruited.mtDNA extracted from peripheral blood were examined by PCR-based assay for D-loop sequence variations,followed by sequencing analysis.Results Compared with the normal control and standard sequence,a total of 61 sequence variants were detected,among these,three high variations were found,and the base variation rate of patients with chronic glomerular nephritis(CGN)and unaffected pedigree members(UAPM)(0.88%,0.72%)significantly increased compared with the control group(0.61%).The distibutional difference of base variation rate in the experimental groups were significantly higher than those in the control group(x2=21.220,7.964,all P<0.05).Especially in microsatellite variation in D310 region,the mutation frequency and base variation rate in patients with CGN were 100.00% and 30.00%,respectively,which of UAPM were 81.30% and 17.95%,respectively,while those in the normal control group were 53.30% and 6.05%,respectively.Among them,the patients with CGN compared with the control group had statistically significant differences(x2=15.610,150.047,all P<0.05).Likewise,UAPM also had statistically significant difference compared with the control group(x2=11.347,66.188,all P<0.05).Conclusion D-Loop of mitochondrial genome mutations may be related to the development of chronic glomerular nephritis.

Objective To observe the clinical efficacy of peritoneal dialysis in the treatment of acute renal failure in children with burns ,and to summarize the experience of treatment .Methods The clinical data of 9 children with burns complicated with acute renal failure who received peritoneal dialysis were retrospectively analyzed . Results On the basis of rehydration and anti -infective therapy , 9 patients underwent continuous bed peritoneal dialysis(CAPD) treatment.The average duration of peritoneal dialysis was 11 days.The average hospital stay was 33 days.The levels of blood urea nitrogen and creatinine were (17.69 ±6.01)mmol/L,(412.21 ±188.74)μmol/L at 5 days after peritoneal dialysis,and the levels of blood urea nitrogen and creatinine were (35.24 ±8.35)mmol/L, (801.23 ±298.15)μmol/L before the peritoneal dialysis,the differences were statistically significant (t =5.12, 3.31,all P<0.01).The levels of blood urea nitrogen and creatinine were (9.76 ±3.37) mmol/L,(168.48 ± 112.25)μmol/L at 10 days after peritoneal dialysis,there were significant improvements compared with 5 days after peritoneal dialysis(t=3.45,3.33,all P<0.01).The symptoms of uremia disappeared or improved obviously ,and the complications of peritoneal dialysis were not obvious .Conclusion Pediatric burns with acute renal failure with dialysis indications preferred peritoneal dialysis treatment ,in the course of treatment should take into account the ade-quacy of dialysis and children with the development of individualized programs .