Objective:To observe the effects of 8-week interval anaerobic training on the respiratory function, airway inflammatoion and exercise performance in the asthmatic children,and to provide theoretic and practical evidence for special exercise prescription for the asthmatic children.Methods:Forty-six mild asthmatic children were willingly divided into exercise group (n=23)and control group (n=23).The children in exercise group conducted 8 weeks interval anaerobic training while the children in control group maintained intrinsical lifestyle.Before and after experiment,the respiratory function,airway inflammatory reaction and exercise performance were determined by pneumonometer,nitric oxide determinator and cycle ergometer.Results:One patient of each group was lost to follow up.509 persons in exercise group finished the training;11 persons had adverse reaction and the incidence was 2.2%.After experiment,compared with before experiment,the peak expiratory flow (PEF)in exercise group after experiment was increased (P <0.05),the fractional exhaled nitric oxide (FeNO)was decreased (P <0.05), the mean power (MP)and peak power (PP)in 30 s Wingate test were raised (P <0.05);there were no significant differences of all indicators in control group (P >0.05).Compared with control group after experiment,all indexes above (PEF,FeNO,MP and PP)in exercise group existed statistical differences (P <0.05).Conclusion:Interval anaerobic training has safetiveness and effectiveness under proper supervision and could improve the respiratory function,reduce the airway inflammation and enhance the exercise performance in the mild asthmatic children.

OBJECTIVEEven though phenylacetate (PA) bas been shown to inhibit the growth and induce differentiation in rat C6 glioma cell line, its mechanisms are still poorly understood. This study is aimed to identify which Hox gene is related to glioma and to observe the change in expression on mRNA level as treated by phenylasetate.

METHODSTwenty-two kinds of Hox gene were divided into 3 groups according to their primer sequence. Semiquantitative reverse transcription- polymerase chain reaction (RT-PCR) was used to investigate the mRNA expression of Hox gene groups and some Hox gene in rat C6 glioma cell line following differentiation induced by PA. The level of Hox gene expression was expressed as ratio expression rate (RER) of Hox gene/beta-actin according to computer image analysis and the difference between C6 cells and PA treated C6 cells was analyzed by student t-test.

RESULTSIt was found that Hox genes matching to primers P2 were mildly expressed in C6 cells and the expression of HoxB2 mRNA was significantly up-regulated in PA treated C6 cells (P < 0.001).

CONCLUSIONThe weak expression of HoxB2 may be involved in glioma origin and the mechanisms of PA action are correlated with transcription process in the glioma cells.

Animals ; Antimetabolites, Antineoplastic ; pharmacology ; Gene Expression Regulation, Neoplastic ; drug effects ; Genes, Homeobox ; genetics ; Glioma ; genetics ; pathology ; Phenylacetates ; pharmacology ; RNA, Messenger ; drug effects ; genetics ; metabolism ; Rats ; Tumor Cells, Cultured ; Up-Regulation ; drug effects