Objective To investigate the serum levels of heat shock protein 70 (HSP70) in uremic patients with different dialysis ages.Methods Ninety-two maintenance haemodialysis patients were divided into short-term group(from three months to 2 years,n =32),median-term group(from 2 to 5 years,n =37) and longterm group(more than 5 years,n =23) according to different dialysis ages.Enzyme-linked immunosorbent assay (ELISA) was used to measure the serum levels of HSP70 before and after haemodialysis in each group.Results The semm levels of HSP70 had significant difference both in median-term group and long-term group before and after haemodialysis(median-term group:(54.94 + 39.21) μg/L vs (69.72 + 39.90) μg/L,t =-2.068,P =0.047 ;long-term group:(46.17 +34.63) μg/L vs (74.07 ±41.11) μg/L,t =-2.814,P =0.010).But there was no statistics difference on the serum levels of HSP70 before and after haemodialysis in the short-term group((70.42 ±38.30) μg/L vs (62.89 ±43.01) μg/L,t =0.870,P =0.390).Conclnsion Haemodialysis patients with more stress protection ability are likely to obtain long term survival.

BACKGROUND:Low concentration of nicotine promotes the angiogenesis and facilitates the healing of skin wounds. However, the role of low concentration of nicotine on the repair of maxil ofacial soft tissue trauma especial y oral mucosa stil remains unclear
OBJECTIVE:To evaluate the effect of low concentration of nicotine on mucosa defect repair of rat hard palate.
METHODS:A circular soft tissue defect at 3 mm diameter was produced in the centre of hard palate of 65 Wistar rats. After the operation, animals were randomly divided into low concentration of nicotine with gel group, gel group and control group. Rats were sacrificed at 3, 7, 10 and 14 days post-surgery. The wound healing was detected with hematoxylin-eosin staining and the difference of wound healing in different groups was compared with gross observation and image measurement.
RESULTS AND CONCLUSION:There was no significant difference in the wound healing in different groups on day 3 post-surgery. On days 7 and 10, the group of low concentrations of nicotine with gel was faster than gel group and control group (P<0.05);the wounds were completely healed on day 14, with no significant difference among the groups. Low concentrations of nicotine may promote the mucosa defects repair of rat hard palate.

[Abstract ] Objective More and more researches demonstrate that epithelial-mesenchymal transition(EMT) is highly rele-vant to cancer metastasis , tissue fibrosis and skin wound healing , but its role in the wound healing of oral mucosa still needs further ex-ploration.This study was designed to discuss the effects of EMT on the wound healing of lingual mucous membrane in rats . Methods A 3mm-diameter circular defect was made on the mucosa of lingual dorsum in 8-week-old Wistar rats.The rats were put to death at day 0, 1, 2, 3, 4 and 8 after operation.We observed tissue healing process by HE staining and used immunohistochemical fluorescence to detect the expressions of EMT-related protein E-cadherin(E-cad), Vimentin and Fibroblast specific proteins (FSP1) in lingual mucous membrane during wound healing . Results The expressions of E-cad at the edge of the wound in the lingual mucous membrane of rats at day 2 and 4 after operation were less than those at day 0 and 1 after operation , while interstitial cell specific protein Vimentin and FSP1 were positively expressed in the epidermal basal layer .The wound of lingual mucous membrane healed completely at day 8 after operation , and E-cad expression was close to that at day 0 after operation .A small amount of Vimentin and FSP 1 expressed in the epi-thelial basal layer . Conclusion E-cad, Vimentin, FSP1 are involved in the wound healing process of lingual mucous membrane . During the wound healing in the tongue mucous membrane of rats , some epithelial cells gain the feature of mesenchymal cells in the process of migration to wound centre .EMT has played an important role during wound healing of lingual mucous membrane in rats .