Objective: To explore the preventive efficacy of local injection of hyaluronidase and topical administration of Hirudoid cream against skin damage caused by vinorelbine extravasation in rats. Methods: Vinorelbine was iv infused into the hinder limbs of SD rats to establish the extravasation model. The 48 rats were randomly divided into 6 groups. The model group received no treatment. The other five groups were given local injection of hyaluronidase, topical administration of Hirudoid cream, local injection of hyaluronidase plus topical administration of Hirudoid cream, local injection of normal saline (NS), or topical administration of normal saline, respectively. The lesion area and the healing time were observed and recorded on d 1, d 4, d 8, d 12, d 18, d 24, and d 30. Results: The lesions were cured by local injection of hyaluronidase on d 30. The lesion area were significantly reduced in hyaluronidase group compared with that in topical Hirudoid cream group, combined therapy group, local NS injection group, topical NS administration group, and the model group on d 1, d 4, d 8, d 12, d 18 and d 24 (P < 0.05). The healing time was significantly shorter in hyaluronidase group than that in other 5 groups [(21.9 ± 3.0) d vs (28.8 ± 3.5) d, (28.0 ± 2.9) d, (28.6 ± 4.1) d, (29.8 ± 2.6) d, and (30. 6 ± 3.0) d, P < 0.01]. Conclusion: Local injection of hyaluronidase is effective for skin damage caused by vinorelbine extravasation, but topical administration of Hirudoid cream is ineffective and combined therapy can not further improve the efficacy of hyaluronidase monotherapy.

Objective: To study the effect of early rehabilitation on motor function of upper and lower extremities and activities of daily living(ADL) in patients with hemiplegia after stroke; and to observe whether motor function recovery of upper and lower extremities is different. Method: 96 patients were randomly divided into two groups, rehabilitation group (48 cases) and control group(48 cases). Prospective method was used in this study. Patients in the rehabilitation group were given clinical treatment and regular rehabilitation training, while those in control group were given clinical treatment and unguided self- training. The evaluation was done in pre- treatment and post- treatment(6- 8 weeks) respectively.Motor function was assessed in Fugl-Meyer assessment(FMA), ADL assessed in Barthel Index. Result: The motor scores (measured by FMA) and ADL scores (measured by Barthel index) in the two groups were improved after 6-8 weeks treatment, but there was a significant difference between the two groups, the scores in the rehabilitation group were obviously superior to those in the control group (P ＜ 0.01 ). The mean of the Fugl-Meyer Scale (transferred to percentage of maximal motor scores) of the lower extremities was higher than that of the upper extremities pre-and post-treatment (P＜0.01). However, the improvement degree of FMA scores between upper and lower extremity had no statistical difference (P＞0.05). Conclusion:Early rehabilitation training of the patients with stroke hemiplegia may obviously improve motor function of the limbs and raise ADL scores. However, the degree of improvement in upper and lower extremity is similar in the first two months after early training.

Adenocarcinoma of exocrine pancreas is a highly malignant tumor with extremely low resectability.Radiotherapy,by inducing necrosis and apoptosis of tumor cells through irradiation effects on cellular DNA,in combination with chemotherapy,has made great contribution to multimodal treatment of this malignancy.The development of radiation therapy for pancreatic carcinoma in recent years was reviewed in neoadjuvant and adjuvant settings,and for locally advanced disease.Further evidence is required to show the impact of radiochemotherapy in the treatment of unresectable disease.

Objective To evaluate the expression of Survivin,PTEN in tongue squamous cell carcinoma (TSCC) and the correlation with vascular endothelial growth factor (VEGF) and its clinical significance.Methods The expression of Survivin,PTEN and VEGF of 72 TSCC samples and 15 normal tongue mucosa (NTM) samples were detected by immunohistochemical method.Results The positive expression rate of Survivin,PTEN and VEGF in TSCC were 66.7％(48/72),61.1％(44/72),70.8％(51/72)respectively,while the positive expression rate in NTM were 0,100.0％ (15/15),0.There were statistical significance between the rate in TSCC and NTM respectively (P ＜0.01 ).The expression of Survivin and VEGF in TSCC were positively correlated with TNM stage,histological grade and lymph node metastasis (P＜ 0.05 or ＜ 0.01 ),while the expression of PTEN had negative correlation with them (P＜ 0.05 or ＜ 0.01 ).The expression of Survivin was positively correlated with VEGF expression in TSCC (r=0.6482,P＜0.01),while the expression of PTEN was negatively correleted with VEGF expression (r =-0.4027,P ＜0.01).Conclusions The expression of Survivin and PTEN in TSCC are associated with tumor occurrence and development closely,and are both significantly correlated with VEGF.Joint detection of Survivin,PTEN and VEGF have important reference value in clinical diagnosis,metastasis and prognosis of TSCC.

Objective To analyze the causative of damaged bioprosthetic valves implanted in 41 patients (with total 47 valves) over one year. Methods From June 1993 to June 2004,re-replacement of valves was performed in 41 patients with dysfunctional bioprosthetic valves. The interval between the two operations was 1～16 years [mean (9.7?4.1) years]. The 47 dysfunctional bioprosthetic valves were carefully observed and pathologically examined. Results Of the 47 valves, no valve frame was damaged; tear or perforation existed in 42 valves (89.3%); calcification was found in 35 valves (74.5%), with fibrosis; excrescence and thrombus coexisted in 2. Heavily damaged ones were sent for pathological examination, and infiltration of abundant phagocytes and monocytes was found. Conclusion Early damages were mainly tear or rupture, while late damages were compound outcomes of multiple damaging factors such as calcification, rupture, and tear.

In order to understand the natural evalution of the motor function and the activities of daily living(ADL) in post stroke patients,and to provide scientific basis for the early treatment and rehabilitation training of the stroke patients,the motor function and ADL of 150 stroke patients without systematic rehabilitation training were evaluated at 5-6 week and 6 month of post stroke,respectively.The results showed that most patients had functional improvements.The functional recovery of upper limbs and especially hands was worse than that of lower limbs.The average ADL scores increased from 59.80 to 69.54,but the improvements were unsatisfactory.The results suggested that it is necessary to carry on the early rehabilitation training for the stroke patients fo that the patients motor function and ADL could improve more quickly and better to greater degree.

Objective:To study the expression of EG-1 in thyroid cancer tissue and analyze its correlation with clinical prognosis. Methods:EG-1 mRNA levels in malignant thyroid tissues were evaluated by RT-PCR. All patients were followed up. The survival rates were analyze in EG-1 positive group and EG-1 negative group. Benign thyroid tissues were used as control. Results:EG-1 was expressed in thyroid cancer. The differences between expression of malignant thyroid tissues and benign thyroid tissues were statistically significant (P

The plant resources, distribution, extraction, pharmacological effects and its application in medicine of flavonoids procyanidins were reviewed based on the literature, in order to provide the basis for further application and comprehensive development.

AIM: To explore the effect of peroxynitrite (ONOO -) on pulmonary microvascular endothelial barrier and roles of ONOO - in the pathogenesis of acute lung injury in vivo. METHODS: SD rats in different groups were insufflated with various concentrations of ONOO -, decomposed ONOO - or vehicle (alkaline normal saline), respectively. Then permeability changes in pulmonary microvascular walls were detected and the pathological alterations of pulmonary tissue were examined under light microscope. Malondialdehyde(MDA) contents were measured in normal lung homogenate pretreated with various concentration of ONOO -. RESULTS: Intratracheal insufflation of ONOO - resulted in dose-dependent increase in lung coefficient, lung wet/dry ratio, lung water contents and Evans blue contents, together with significant pulmonary pathological changes such as diffuse alveolar collapse, capillary congestion, focal hemorrhage, and endothelial swollen. In addition, ONOO - can also elicit increase in MDA contents in normal lung homogenate. CONCLUSION: ONOO - may induce dysfunctions of pulmonary microvascular endothelial barriers, it is suggested that enhanced endogenous ONOO - generation may take part in the pathogenesis of acute lung injury.

AIM: To elucidate the anti-inflammatory mechanism of cholecystokinin octapeptide (CCK-8). METHODS: The pulmonary interstitial macrophages (PIMs) from rats were stimulated with LPS (1 mg?L~(-1)) in the presence or absence of CCK-8 (10~(-8)-10~(-6) mol?L~(-1)) or/and CCK receptor antagonist proglumide (2 mg?L~(-1)). The expression of TNF-? mRNA was assayed by reverse transcription polymerase chain reaction (RT-PCR) at 3 h of the stimulation, and nuclear factor-?B (NF-?B) binding activity was analyzed by electrophoretic mobility shift assay (EMSA) at 1 h of stimulation. The I?B? protein level in the cytoplasma at 30 min of the stimulation was detected by Western blot. RESULTS: CCK-8, at concentrations from 10~(-8) mol?L~(-1) to 10~(-6) mol?L~(-1) obviously inhibited LPS-induced TNF-? mRNA expression and NF-?B binding activity in a dose-dependent manner. Stimulation with LPS resulted in a reduction of I?B? protein level in PIMs, which was elevated by CCK-8. The effects of CCK-8 on NF-?B activity and I?B protein level were attenuated by CCK receptor antagonist proglumide. CONCLUSION: CCK-8 inhibits LPS-induced TNF-? mRNA expression by regulating NF-?B activity in rat PIMs, which is mediated through CCK receptors and inhibition of I?B? degradation. This represents one of the anti-inflammatory mechanisms of CCK-8.