Objective: To explore the preventive efficacy of local injection of hyaluronidase and topical administration of Hirudoid cream against skin damage caused by vinorelbine extravasation in rats. Methods: Vinorelbine was iv infused into the hinder limbs of SD rats to establish the extravasation model. The 48 rats were randomly divided into 6 groups. The model group received no treatment. The other five groups were given local injection of hyaluronidase, topical administration of Hirudoid cream, local injection of hyaluronidase plus topical administration of Hirudoid cream, local injection of normal saline (NS), or topical administration of normal saline, respectively. The lesion area and the healing time were observed and recorded on d 1, d 4, d 8, d 12, d 18, d 24, and d 30. Results: The lesions were cured by local injection of hyaluronidase on d 30. The lesion area were significantly reduced in hyaluronidase group compared with that in topical Hirudoid cream group, combined therapy group, local NS injection group, topical NS administration group, and the model group on d 1, d 4, d 8, d 12, d 18 and d 24 (P < 0.05). The healing time was significantly shorter in hyaluronidase group than that in other 5 groups [(21.9 ± 3.0) d vs (28.8 ± 3.5) d, (28.0 ± 2.9) d, (28.6 ± 4.1) d, (29.8 ± 2.6) d, and (30. 6 ± 3.0) d, P < 0.01]. Conclusion: Local injection of hyaluronidase is effective for skin damage caused by vinorelbine extravasation, but topical administration of Hirudoid cream is ineffective and combined therapy can not further improve the efficacy of hyaluronidase monotherapy.

Objective To analyze the causative of damaged bioprosthetic valves implanted in 41 patients (with total 47 valves) over one year. Methods From June 1993 to June 2004,re-replacement of valves was performed in 41 patients with dysfunctional bioprosthetic valves. The interval between the two operations was 1～16 years [mean (9.7?4.1) years]. The 47 dysfunctional bioprosthetic valves were carefully observed and pathologically examined. Results Of the 47 valves, no valve frame was damaged; tear or perforation existed in 42 valves (89.3%); calcification was found in 35 valves (74.5%), with fibrosis; excrescence and thrombus coexisted in 2. Heavily damaged ones were sent for pathological examination, and infiltration of abundant phagocytes and monocytes was found. Conclusion Early damages were mainly tear or rupture, while late damages were compound outcomes of multiple damaging factors such as calcification, rupture, and tear.

Adenocarcinoma of exocrine pancreas is a highly malignant tumor with extremely low resectability.Radiotherapy,by inducing necrosis and apoptosis of tumor cells through irradiation effects on cellular DNA,in combination with chemotherapy,has made great contribution to multimodal treatment of this malignancy.The development of radiation therapy for pancreatic carcinoma in recent years was reviewed in neoadjuvant and adjuvant settings,and for locally advanced disease.Further evidence is required to show the impact of radiochemotherapy in the treatment of unresectable disease.

Objective To evaluate the expression of Survivin,PTEN in tongue squamous cell carcinoma (TSCC) and the correlation with vascular endothelial growth factor (VEGF) and its clinical significance.Methods The expression of Survivin,PTEN and VEGF of 72 TSCC samples and 15 normal tongue mucosa (NTM) samples were detected by immunohistochemical method.Results The positive expression rate of Survivin,PTEN and VEGF in TSCC were 66.7％(48/72),61.1％(44/72),70.8％(51/72)respectively,while the positive expression rate in NTM were 0,100.0％ (15/15),0.There were statistical significance between the rate in TSCC and NTM respectively (P ＜0.01 ).The expression of Survivin and VEGF in TSCC were positively correlated with TNM stage,histological grade and lymph node metastasis (P＜ 0.05 or ＜ 0.01 ),while the expression of PTEN had negative correlation with them (P＜ 0.05 or ＜ 0.01 ).The expression of Survivin was positively correlated with VEGF expression in TSCC (r=0.6482,P＜0.01),while the expression of PTEN was negatively correleted with VEGF expression (r =-0.4027,P ＜0.01).Conclusions The expression of Survivin and PTEN in TSCC are associated with tumor occurrence and development closely,and are both significantly correlated with VEGF.Joint detection of Survivin,PTEN and VEGF have important reference value in clinical diagnosis,metastasis and prognosis of TSCC.

Objective: To study the effect of early rehabilitation on motor function of upper and lower extremities and activities of daily living(ADL) in patients with hemiplegia after stroke; and to observe whether motor function recovery of upper and lower extremities is different. Method: 96 patients were randomly divided into two groups, rehabilitation group (48 cases) and control group(48 cases). Prospective method was used in this study. Patients in the rehabilitation group were given clinical treatment and regular rehabilitation training, while those in control group were given clinical treatment and unguided self- training. The evaluation was done in pre- treatment and post- treatment(6- 8 weeks) respectively.Motor function was assessed in Fugl-Meyer assessment(FMA), ADL assessed in Barthel Index. Result: The motor scores (measured by FMA) and ADL scores (measured by Barthel index) in the two groups were improved after 6-8 weeks treatment, but there was a significant difference between the two groups, the scores in the rehabilitation group were obviously superior to those in the control group (P ＜ 0.01 ). The mean of the Fugl-Meyer Scale (transferred to percentage of maximal motor scores) of the lower extremities was higher than that of the upper extremities pre-and post-treatment (P＜0.01). However, the improvement degree of FMA scores between upper and lower extremity had no statistical difference (P＞0.05). Conclusion:Early rehabilitation training of the patients with stroke hemiplegia may obviously improve motor function of the limbs and raise ADL scores. However, the degree of improvement in upper and lower extremity is similar in the first two months after early training.

In order to understand the natural evalution of the motor function and the activities of daily living(ADL) in post stroke patients,and to provide scientific basis for the early treatment and rehabilitation training of the stroke patients,the motor function and ADL of 150 stroke patients without systematic rehabilitation training were evaluated at 5-6 week and 6 month of post stroke,respectively.The results showed that most patients had functional improvements.The functional recovery of upper limbs and especially hands was worse than that of lower limbs.The average ADL scores increased from 59.80 to 69.54,but the improvements were unsatisfactory.The results suggested that it is necessary to carry on the early rehabilitation training for the stroke patients fo that the patients motor function and ADL could improve more quickly and better to greater degree.

BACKGROUND:Compared with bone marrow transplantation, peripheral blood stem cel transplantation has its own advantages, including rich resources of stem cel s from the peripheral blood, convenient and easy col ection, without anesthesia, smal trauma, easily accepted, high safety, and easy to restore the patient’s hematopoietic system.
OBJECTIVE:To observe the function and safety of autologous peripheral blood mononuclear cel s in the treatment of patients with decompensated cirrhosis.
METHODS:Four patients with decompensated liver cirrhosis were selected from November 2010 to July 2011 in the First Affiliated Hospital of Dalian Medical University, aged 31-67 (averagely 44 years). Among them, three cases had hepatitis B, and another one had autoimmune liver disease. Peripheral blood stem cel s were col ected after being mobilized by granulocyte colony stimulating factor. Then, autologous peripheral blood stem cel s were transplanted via a hepatic artery catheter.
RESULTS AND CONCLUSION:There were no adverse reactions such as fever, bleeding and nausea after peripheral blood stem cel col ection and hepatic artery transplantation. Symptoms such as fatigue, poor appetite and abdominal distension gradual y improved at 1, 3 and 6 months after transplantation. Liver function and liver fibrosis indexes were improved to some extent after transplantation.

AIM:The method of HPLC was established for the analysis of fingerprint of Compound Danshen Tablet (Radix et Rhizoma Salvial miltiorrhizal,Radix et Rhizoma Notoginseng,Borneolum Syntheticum) and the determination of its main components. METHODS:The fingerprint chromatograms of liposoluble constituents of Compound Danshen Tablet were set up and its main components of 10 batches were determined. RESULTS:The fingerprint chromatograms were simulated with mean method and the analysis of different batches of Compound Danshen Tablet was made after the analysis of the fingerprint chromatograms from different groups of Compound Danshen Tablet. The similarities which were proved to be higher than 0.99 were calculated with the help of The Similarity Calculation Soft of Fingerprint Chromatography of Traditional Chinese Medicin. CONCLUSION:This method is accurate and provides a scientific basis for controlling the quality of Compound Danshen Tablet.

Objective:To study the expression of EG-1 in thyroid cancer tissue and analyze its correlation with clinical prognosis. Methods:EG-1 mRNA levels in malignant thyroid tissues were evaluated by RT-PCR. All patients were followed up. The survival rates were analyze in EG-1 positive group and EG-1 negative group. Benign thyroid tissues were used as control. Results:EG-1 was expressed in thyroid cancer. The differences between expression of malignant thyroid tissues and benign thyroid tissues were statistically significant (P

Objective: To investigate the antitumor effect of osthole on human B-cell acute lymphoblastic leukemia (B-ALL) 697 cells and its possible mechanism. Methods: After B-ALL 697 cells were treated with different concentrations (8, 16, 32, 64 and 128 μmol/L) of osthole, the inhibition rate of cell proliferation was detected by CCK-8 assay. After B-ALL 697 cells were treated with 8 and 32 μmol/L osthole, the apoptosis was detected by FCM, the expressions of apoptosis-associated molecule Bcl-2 and Bax were detected by real-time fluorescent quantitative PCR and Western blotting. After B-ALL 697 cells were treated with 8 and 32 μmol/L osthole alone or in combination with autophagy inhibitor 3-methyladenine (3-MA), the intracellular mean fluorescent intensity (MFI) was detected by FCM [stained by monodansylcadaverine (MDC)] to reflect the autophagy. The expressions of autophagy-associated molecule Beclin 1 mRNA and protein was detected by real-time fluorescent quantitative PCR and Western blotting, respectively. Results: The proliferation of B-ALL 697 cells in 8, 16, 32, 64 or 128 μmol/L osthole treatment group was significantly inhibited in a dose-and time-dependent manner (all P < 0.05). The apoptosis rate of B-ALL 697 cells in 8 or 32 μmol/L osthole treatment group was significantly increased (both P < 0.01), the expression levels of Bax mRNA and protein were increased (all P < 0.05), while the expression levels of Bcl-2 mRNA and protein were decreased (all P < 0.05). The MDC MFI of B-ALL 697 cells in 8 or 32 μmol/L osthole treatment group was increased (both P < 0.01), and the expression levels of Beclin 1 mRNA and protein were significantly increased (all P < 0.05). There was no significant change in MDC MFI and the expressions of Beclin 1 mRNA and protein after treatment with 3-MA in combination with osthole (all P > 0.05). Conclusion: Osthole inhibits the proliferation, and induces the apoptosis and autophagy of B-ALL 697 cells. The mechanism of promoting apoptosis may be related to the up-regulation of Bax expression and the down-regulation of Bcl-2 expression. Beclin 1 participates in the autophagy.