Combretum species (combretaceae) are widely used as folk medicine for the treatment of hepatitis, malaria, respiratory infection and even cancer in different parts of Asia and Africa. Claimed efficacies in Thai traditional textbooks are as follows that the leaves of Combretum quadrangulare Kurz was have been used as a treatment of wounds. In this study, we researched the action of Tram bau on hepatotoxicity. The centrilobular localization of CCl4 induced hepatic necrosis. The results showed that Trambau decoction with dosage 0,2 ml recovered the hepatic injury.
Recovery of Function ; Poisoning ; Infection ; Combretaceae ; Hepatocytes

OBJECTIVETo establish a IE-HPLC method for the determination of trigonelline in the fruit and seed of Quisqualis indica.

METHODThe method was carried out by using Sperisorb SCX column with a mobile phase consisting of 20 mmol x L(-1) phorsphate (pH3.2, titrate a 20 mmol x L(-1) monsodium salt with a 20 mmol x L(-1) solution of phosphoric acid), flow rate of 1.0 mL x min(-1), and the detection at 265 nm.

RESULTBy using SCX-modified silica column, trigonelline in Fructus Quisqualis can be baseline separated and determinated. The retention mechanisms proved to be contributed mainly by ion-exchange with the SCX moieties. The validity of methods was demonstrated with respect to linearity, precision, reproducibility and recovery. It was found that there was no trigonelline occurring in the peel of Fructus Quisqualis.

CONCLUSIONThis assay method can be used for the quality control of Fructus Quisqualis.

Alkaloids ; analysis ; Chromatography, High Pressure Liquid ; methods ; Combretaceae ; chemistry ; Fruit ; chemistry ; Plants, Medicinal ; chemistry ; Quality Control ; Seeds ; chemistry