1.Enhancing stability and activity of β-galactosidase from Kluyveromyces lactis through immobilization on polymethacrylate monolith and optimisation using response surface methodology
Rima Fatira Dahari ; Mailin Misson ; Clarence M Ongkudon ; Suryani Saallah ; Aliyah Madihah Asran ; Daniel Joe Dailin ; Lai Fatt Chuah ; Hesham El Enshasy
Malaysian Journal of Microbiology 2023;19(no.6):752-765
Aims:
This study investigates the potential of porous polymethacrylate monoliths as enzyme support materials for large-scale enzyme commercialization.
Methodology and results:
It focuses on their preparation and various immobilization techniques, such as adsorption, covalent-binding and cross-linking, specifically applied to β-galactosidase for bioprocess applications. The research assesses immobilization performance, operational stability, reusability and optimization using response surface methodology (RSM). The results reveal that covalent-binding exhibited the highest enzyme activity recovery, while cross-linking showed superior performance at lower enzyme concentrations but decreased at higher concentrations. Covalent-bound enzymes demonstrated reusability for up to four cycles, with optimal pH ranging between 7 and 8 and optimal temperature ranging between 30 °C and 40 °C. Furthermore, RSM optimization highlighted the significant influence of substrate concentration on enzyme activity, with a reliable model (R2 = 0.9163) and adequate precision (S/N = 13.1409).
Conclusion, significance and impact of study
Overall, this study provides valuable guidelines for effectively employing porous monoliths in large-scale industrial bioprocessing, offering potential cost-saving benefits and enhanced efficiency in enzyme commercialization.