Introduction: Catfish oil contains saturated and unsaturated fatty acids. Polyunsaturated fatty acid contributes to maintain the lipid profile. Enterococcus faecium IS-27526 is a novel indigenous probiotic of dadih origin. The aim of this study was to find out the effect of probiotic and catfish (Clarias gariepinus) based functional feed on lipid profile of aged female Cynomolgus monkey/CM (Macaca fascicularis) fed with atherogenic diet. Methods: Nine CMs were randomly devided into 3 groups, in single blind intervention study for 90 days , namely: A1= atherogenic diet as control positive, A2= probiotic, A3= probiotic + catfish oil after 45 days adaptation period. The average of CM’s bodyweight was in a range of 3 to 4 kg. The main ingredients of atherogenic diet were catfish flour, egg, sugar, egg yolk flour, and condiments. Serum lipid profile was assessed monthly by Cholesterol Oxidase-Peroxidase Aminoantipyrine (CHOP-POD) methods for cholesterol, LDL and HDL, while Glycerol-3-Phosphate Oxidase Peroxidase-Antiperoxidase (GPO-PAP) method for Triglyceride. Results: Atherogenic diet did not significantly affect appetite and bodyweight of the animals (p=0,12). The atherogenic diet significantly increased cholesterol and LDL level of animals (p=0.03 and p = 0.04) after 90 days administration . The CMs fed with probiotic E. faecium IS-27526 and catfish oil showed significant higher serum cholesterol (p=0,03) and LDL levels (p=0,04) compared to atherogenic diet alone and atherogic diet with probiotic E. faecium IS-27526. Probiotic E. faecium IS-27526 at 108 cfu/day stabilized the cholesterol and LDL level after 90 days administration compared with other groups of intervention. The TG and HDL of each group showed within normal range during the whole intervention study. Conclusion: Atherogenic feed significantly increased cholesterol and LDL level of CMs. Probiotic E. faecium IS-27526 showed a potential beneficial effect in maintaining lipid profile and could prevent dyslipidemia.