Pro.Shan Zhaowei is the well-known expert of splenic-gastric disease,learning from famous teacher,with rich experience and good skill,strict with drugs administration,esp.in clinically applying rutaceae and other drugs regulating Qi flow.

AIM: To study and analyze the clinical effect of phacoemulsification and intraocular lens(IOL)implantation combined with goniosychialysis in treatment of acute angle-closure glaucoma with cataract.

METHODS: From June 2010 to October 2018, 40 patients(40 eyes)with acute angle-closure glaucoma and cataract which anterior chamber angle adhesion is less than 180°admitted to our hospital were enrolled in this study. They were divided into observation group and control group according to different surgical methods. The patients in the control group were treated by phacoemulsification and IOL implantation. The observation group was treated with phacoemulsification and IOL implantation combined with goniosychialysis. The difference between the two groups was compared.

RESULTS: At 3mo, 6mo after operation, the visual acuity, intraocular pressure, anterior chamber depth, anterior chamber angle grading of the observation group were better than those of the control group(P<0.05).

CONCLUSION: Regarding the treatment of acute angle closure glaucoma, who has cataract and less than 180° peripheral anterior synechia, phacoemulsification and IOL implantation combined with goniosychialysis showed significant clinical effect.

OBJECTIVETo compare the clinical efficacy differences between "governor vessel method of acupuncture" combined with estazolam and estazolam alone for insomnia.

METHODSSeventy patients of insomnia were randomly divided into an observation group and a control group, 35 cases in each one. The patients in the control group were treated with oral administration of estazolam, 2 mg each time; the estazolam was taken 30 min before sleeping. The patients in the observation group were treated with "governor vessel method of acupuncture" combined with estazolam; the positive reaction points along governor vessel and Baihui (GV 20) were selected as the main points. The acupuncture was given for 30 min per treatment, once every two days. Both groups were treated for four weeks. Pittsburgh sleep quality index (PSQI) and polysomnography (PSG) were observed before and after treatment. Clinical efficacy was assessed between the two groups and adverse reactions were recorded.

RESULTSThe total effective rate was 94.3% (33/35) in the observation group, which had no significant difference with 82.9% (29/35) in the control group (>0.05). The cured and markedly effective rate was 71.4% (25/35) in the observation group, which was superior to 42.9% (15/35) in the control group (<0.05). Each item score and total score of PSQI were decreased obviously after treatment in the observation group (all <0.05), while the score of sleep latency, sleep duration and sleep efficiency as well as total score of PSQI were decreased obviously after treatment in the control group (all <0.05). The scores of sleep duration, sleep disturbance and day dysfunction due to sleepiness as well as total score of PSQI in the observation group were lower than that in the control group (all <0.05). After treatment, the PSG-related parameters of total sleeping duration, sleeping latency, sleeping arousal and sleeping efficiency were improved significantly in the observation group (all <0.05); the total sleeping duration, sleeping arousal and sleeping efficiency were also improved in the control group (all <0.05). The total sleeping duration, sleeping latency, sleeping arousal and sleeping efficiency in the observation group were superior to those in the control group (all <0.05). 4 adverse reactions in the observation group and 3 adverse reactions in the control group were observed.

CONCLUSION"Governor vessel method of acupuncture" combined with estazolam could significantly improve insomnia, which are superior to estazolam alone.

There are many pathogenic correlation factors of chronic refractory wound. Due to the complexity and particularity of the causes of wounds and lack of a standard diagnosis guide, it is hard to treat this kind of wound. Based on our recent scientific research data and the relative research at home and abroad in the present, we systematically analyze and summarize the causes and non-surgical treatment of chronic refractory wound in this article.

The treatment of mass burn patients is related to social stability, life saving, and disability reducing. It is also an important opportunity to accumulate, summarize, and improve clinical rescue and treatment experience. Aiming at August 2nd Kunshan factory aluminum dust explosion, this article reviews and summarizes experience and problems about rescue reserve, first-aid system, multidisciplinary cooperation, and integration of usual time and emergent time treatment, so as to propose corresponding strategies and provide reference for the treatment of mass burn patients and critically burned patients.

Objective: To explore the changes of helper T lymphocytes 17 (Th17) and regulatory T lymphocytes (Tregs) in peripheral blood of patients with extensive burn at early stage in August 2nd aluminum dust explosion accident in Kunshan factory (hereinafter referred to as Kunshan explosion accident) and the significance. Methods: Twenty patients with extensive burn in Kunshan explosion accident admitted to our department of burns and plastic surgery were enrolled in burn group, and 10 healthy adult volunteers with no history of smoking were enrolled in healthy control group. Five mL of peripheral venous blood samples were collected from patients in burn group at admission (≤post injury hour 6, PIH 6) and PIH 24, and on post injury day (PID) 7, and from volunteers in healthy control group respectively. The percentages of CD64⁺ T lymphocytes, human leukocyte antigen-DR positive (HLA-DR⁺ ) T lymphocytes, CD3⁺ CD8^- Th17, and CD4⁺ CD25⁺ Tregs in peripheral blood T lymphocytes were determined by flow cytometer. Results: (1) The percentages of CD64⁺ T lymphocytes in peripheral blood T lymphocytes of patients in burn group at admission and PIH 24 [(2.35±0.32)% and (4.02±0.15)%] were higher than (0.67±0.11)% of healthy volunteers in healthy control group. The percentage of CD64⁺ T lymphocytes in peripheral blood T lymphocytes of patients in burn group on PID 7 [(0.39±0.25)% ] was lower than that of healthy volunteers in healthy control group. The percentages of HLA-DR⁺ T lymphocytes in peripheral blood T lymphocytes of patients in burn group at admission and PIH 24 [(54±18)% and (72±17)%] were higher than (38±14)% of healthy volunteers in healthy control group. The percentage of HLA-DR⁺ T lymphocytes in peripheral blood T lymphocytes of patients in burn group on PID 7 [(28±15)% ] was lower than that of healthy volunteers in healthy control group. (2) The percentages of CD3⁺ CD8^-Th17 in peripheral blood T lymphocytes of patients in burn group at admission and PIH 24 [(4.21±0.46)% and (7.38±0.39)%] were higher than (0.98±0.09)% of healthy volunteers in healthy control group. The percentage of CD3⁺ CD8^-Th17 in peripheral blood T lymphocytes of patients in burn group on PID 7 [(0.81±0.05)% ] was lower than that of healthy volunteers in healthy control group. (3) The percentages of CD4⁺ CD25⁺ Tregs in peripheral blood T lymphocytes of patients in burn group at admission and PIH 24, and on PID 7 [(1.78±0.26)%, (1.26±0.37)%, and (0.38±0.03)%] were lower than (3.24±0.52)% of healthy volunteers in healthy control group. Conclusions The percentage of Th17 in peripheral blood T lymphocytes of patients with extensive burn at early stage in Kunshan explosion accident increased at first and then decreased compared with that of healthy volunteers, indicating that the patients′ immune function increased first and then weakened. The percentages of Tregs in peripheral blood T lymphocytes at each time point were lower than the percentage of healthy volunteers, indicating that the patients′ immunomodulation gradually weakened after extensive burns.

Collagen, as the main structural protein in human body, plays an important role in the wound healing process. Due to their inherent hemostatic characteristics, good biocompatibility, low immunogenicity, as well as controllable biodegradability, collagen-based materials have attracted much attention. In this article, we mainly introduce the characteristics of collagen-based wound biomaterials and the application mechanism as scaffold and wound dressing. And the standard and unified experts' consensus formed on the clinical indications, recommended applications, contraindications, and matters needing attention. The consensus wound help clinicians and patients to recognize collagen-based wound biomaterials correctly and use them rationally.
Animals ; Bandages ; Biocompatible Materials ; Collagen ; Consensus ; Humans ; Wound Healing

The mental disorders, scar, and dysfunction will affect the work and study of patients with extensive burn, which can further affect the quality of life and the rate of return to society. Under the premise of saving life, the function of patients with extensive burn should be reserved as much as possible for a better living quality. In addition to the specialized treatment, body positioning, therapeutic exercise, orthosis application, and psychotherapy can be used in the early stage of burn rehabilitation. Early rehabilitation training is beneficial to the circulatory system, immune system, functional and psychological recovery of patients. It can also improve the quality of extensive burn treatment, and decrease deformities and the incidence of complications in the late stage.

Mesenchymal stem cells possess the ability of self-renewal and multiple differentiation potential, thus exert immunomodulatory effect during tissue repair. Mesenchymal stem cells can stimulate angiogenesis and promote tissue repair through transdifferentiation and secreting a variety of growth factors and cytokines. This review outlines the advances in the mechanism of mesenchymal stem cells in promoting wound healing, including alleviation of inflammatory response, induction of angiogenesis, and promotion of migration of mesenchymal stem cells to the site of tissue injury.
Cell Differentiation ; Cell Transdifferentiation ; Mesenchymal Stem Cell Transplantation ; methods ; Mesenchymal Stromal Cells ; cytology ; metabolism ; physiology ; Skin ; cytology ; metabolism ; Wound Healing ; physiology

Objective: To investigate the molecular mechanism of lncRNA FOXD2-AS1 participating in apatinib resistance in gastric cancer cells by regulating miR-185-5p/CCND2 axis. Methods: The gastri cancer tissues and corresponding paracancerous tissues of 25 patients with gastric cancer were collected from April 2016 to December 2017 in the Fifth People’s Hospital of Wuxi City. The expressions of FOXD2-AS1, miR-185-5p, and cyclin D2 (CCND2) in gastric cancer tissues or cell lines were examined by quantitative realtime polymerase chain reaction (qRT-PCR). CCK-8 assay, Transwell assay and Annexin V-FITC/PI double staining flow cytometry assay were applied to assess the sensitivity of gastric cancer cells to apatinib. The interaction between FOXD2-AS1, miR-185-5p and CCND2 was explored by dual luciferase reporter gene assay, which was then confirmed by qRT-PCR, and Western blotting. Results: FOXD2-AS1 was highly expressed in gastric cancer tissues and apatinib-resistant gastric cancer cells. Over-expression of FOXD2-AS1 promoted apatinib-resistance of MGC-803/AP cells. Dual luciferase reporter gene assay confirmed that FOXD2-AS1 directly interacted with miR-185-5p and suppressed its expression. miR-185-5p significantly abolished the promotion effect of FOXD2-AS1 on apatinibresistance via inhibiting cell proliferation, invasion and promoting apoptosis of gastric MGC-803/AP cells. miR-185-5p could negatively regulate CCND2 expression; and FOXD2-AS1 promoted the cell proliferation, invasion and inhibited apoptosis of MGC-803/AP cells via down-regulating the inhibition effect of miR-185-5p on CCND2, thus further enhanced the apatinib-resistance of gastric cancer cells. Conclusion: FOXD2-AS1 induced apatinib-resistance of gastric cancer cells by regulating miR-185-5p/CCND2 axis.