Objective To knockout the murine Txnip gene using microinjection of transcription activator-like effector nuclease ( TALEN) mRNAs.Methods TALEN knockout site recognizing Txnip was designed by tools on line, then constructed the vectors and assayed its cleavage activity at cellular level.TALEN mRNA was transcribed in vitro and microinjected into C57BL/6J mouse zygotes.F0 mice were verified at DNA level with BamHI and TXNIP-knockout mice were obtained.Results We designed and constructed TALENs which recognized and cut the first exon of Txnip, and got four TXNIP knockout mice, among which two were frameshift mutation, demonstrating that the TXNIP-knockout mice were generated by TALEN technique.Conclusions Microinjection of in vitro transcribed TALEN mRNAs into murine zygotes is a highly effective and convenient way to develop TXNIP-knockout mouse model.

Background: Although the association of single nucleotide polymorphisms (SNPs) of cyclooxygenase (COX) genes and the risk of aspirin resistance (AR) has been extensively studied, the results remain conflicting. The majority of studies have focused on the role of rs20417 (COX-2 -G765C) in AR. To derive a more comprehensive and accurate evaluation of this association, we performed a meta-analysis including the most recent studies. Methods: Relevant studies published up to October 2018 were identified by searching the PubMed, EMBASE, Web of Science, Cochrane, China Nation Knowledge Infrastructure Platform, Wanfang, and VIP databases, and by manual searching reference lists of the retrieved articles. Odds ratios (ORs) and 95% confidence intervals (CIs) were applied to assess the strength of associations. Sensitivity and subgroup analyses were performed to explore the stability of results and between-study heterogeneity, respectively. Results: A total of 18 studies on rs20417 were pooled into the meta-analysis. Rs20417 was found to be associated with an increased risk of AR (C vs. G: OR = 1.43, 95% CI = 1.10–1.86, p < 0.05; GC+CC vs. GG: OR = 1.54, 95% CI =1.15–2.05, p < 0.05). These associations were stronger in Chinese participants and in patients with ischemic stroke in subgroup analyses. Conclusion: The presence of rs20417 indicates an increased risk of AR, especially in Chinese participants and patients with ischemic stroke. This association could help to improve personalized medicine and initiate appropriate treatment as necessary. Further large-scale studies are warranted to confirm our findings.