Objective To analyze the curative effect of neoadjuvant chemotherapy combined with limb salvage for osteosarcoma with pathologic fracture.Methods The cases were divided into combined group (68 cases of osteosarcoma with pathologic fracture who received limb salvage after neoadjuvant chemotherapy),non-chemotherapy group (22 cases of osteosarcoma with pathologic fracture who received operation without concurrent chemotherapy or chemotherapy only after operation),non-fracture group (48 cases of osteosarcoma without synchronous pathologic fracture who received limb salvage after neoadjuvant chemotherapy) and amputation group (32 cases of osteosarcoma combined with pathologic fracture who received amputation).Survival rate,local recurrence rate,metastasis rate and quality of life were compared between groups.Results Average estimated survival period of the combined group was obviously longer than that of the non-chemotherapy group (P ＜ 0.05),but had no significant difference from the non-fracture group or amputation group.Of the 5-year survival rate between groups,the combined group was evidently higher than the non-chemotherapy group (P ＜ 0.05),but without significant difference from the non-fracture group or amputation group.Whereas,non-chemotherapy group showed a lower 5-year survival rate than the amputation group or non-fracture group (P ＜ 0.05).Localized recurrence rate and metastasis rate of the combined group were markedly decreased when compared to the nonchemotherapy group (both P ＜ 0.01),but were not significantly different from the non-fracture group or the amputation group.The combined group showed a significantly higher score of social function than the amputation group (P ＜0.01),a significantly higher score of biological pofession than the non-chemotherapy group or the amputation group (P ＜ 0.05),and a significantly higher score of general health than the nonchemotherapy group (P ＜ 0.05).However,no significant differences were observed between groups regarding the scores for other items.Conclusion New adjuvant chemotherapy combined with limb salvage is a rational treatment of osteosarcoma with pathologic fracture for it saves the limb after the guarantee of survival rate without increasing the localized recurrence rate or metastasis rate,and improves quality of life.

Objective To investigate apoptosis of gastric cancer cells induced by taxol,and its specific apoptotic cell cycle phase. Methods The apoptosis rate of gastric cancer cell line MKN-28 induced by taxol was detected with Sub-G1 method.The specific apoptotic cell cycle phase Was detected with API and PSC method.The sensitivity of 20 cases clinic gastric cancer specimens induced by taxol was detected with the method MTT.Results With the Sub-G1 method the MKN-28 cells were induced by 10 μg/ml taxol,after 10 h,the apoptosis rate reached its top apex;with the API method and the PSC method,the specific apoptosts took place in G_2/M phase;the chemotherapy sensitivity of 16 cases out of 20 cases clinic gastnc cancer specimens exceed 50%with the method MTT. Conclusion Taxol could induce gastric cancer cells to aimptosis and the apoptosis takes place in G_2/M phase;Taxol is sensitive to clinic gastric cancer speclmens.

Objective To analyze the treatment methods of the mesh infection after tension-free repair of inguinal hernia. Methods The clinical data of 6 patients with mesh infection after tension-free repair of inguinal hernia from January 2012 to December 2017 were retrospectively analyzed. The materials of the meshes were standard knitted polypropylene. Transabdominal preperitoneal technique (TAPP) was performed in 1 case, modified Kugel repair was performed in 1 case, and Lichtenstein repair was used in 4 cases. Five patients accepted reoperation, and the infected meshes were removed combined with closed drainage. One patient accepted conservative treatment. Results The patients who received the reoperation all healed and were discharged without perioperative death; the patients were followed up 6 to 40 months (average 19 months), and there were no recurrence patients. The patient with conservative treatment developed incision chronic infectious sinus formation. Conclusions There are many reasons for mesh infection after tension-free repair of inguinal hernia, and the management should be individual. Once the infection occurs, the infected mesh should be removed timely so as to shorten the course of disease, but the key is prevention.