An investigation was made on the rehabilitation treatment for the injuries wounded in Wenchuan Earthquake offered in 30 hospitals of 8 provinces, including Beijing, Shanghai, Jiangsu, Shandong, Hubei, Guangdong, Cbongqing, and Sichuan. On the basis of data from this investigation, the paper made analysis and summary of the challenges and problems encountered in the said rehabilitation treatment, and raises such recommendations as relessing rehabilitation assessment principles and policies for the injuries to return to their home towns in Siehuan, clarifying the responsibilities of individual government authorities in the later-stage rehabilitation treatment of the injuries, and enhanced synergy among respective disciplines and authorities of both clinical practice and rehabilitation treatment. The goal of these recommendations is further development of the rehabilitation medicine discipline in China.

Objective To evaluate the relationship between ischemic cerebrovascular desease(ICVD) and carotid atherosclerosis and its risk factors.Methods 186 cases with ICVD and 194 cases without cerebrovascular disease or healthy people(control group) were enrolled in this study.Carotid ultrasonagraphy was performed in the two groups and blood biochemical indexes were detected in the meanwhile.The data were compared between the two groups.Results Higher age(69?7years) and higher prevalence of hypertension(66.1%),diabets mellitus(53.4%) and metabolic syndrome(44.6%) were found in ICVD group than in control groups [(61?5)years,48.8%,15.2%,12.9%](all(P

Endoscopic ultrasound was initially introduced in 1980s as a diagnostic modality combining echoendoscopes with radial scanning, which permitted reconstruction of cross-sectional images similar to computed tomography. Many studies showed that endogastric ultrasonography is more accurate than endoscopy or CT in evaluating the depth of invasion ( T category) and the involvement of lymph nodes (N category) in patients with gastric carcinoma. In addition to being very useful in tissue acquisition for diagnostic purposes, the principles behind endoscopic ultrasound-guided fine needle aspiration ( FNA) paved the way for the development of therapeutic endoscopic ultrasound, which improve the prognosis of patients with gastric cancer.

Objective To understand the prevalence of physical activity deficiency and its association with depressive, anxiety symptoms and dissatisfaction of school life among adolescents in Shaoxing, Zhejiang province. Method A total of 3 397 valid questionnaires from adolescents from 80 classes of 2 middle schools (including 1 420 urban and 1 977 town adolescents) were investigated for the study, their average age was (13.73±1.01) years. The Depression Self-rating Scale for Children (DSRSC) and Screen for Child Anxiety Related Emotional Disorders (SCARED) were used to assess the mental health status, while the dissatisfaction of school life was evaluated by the School Life Satisfaction Rating Questionnaire for Adolescent (SLRQA), and the data were analyzed by descriptive statistics and multivariate logistic regression models. Result The self-reported proportion of 3 397 adolescents with physical activity deficiency reached 78.5%, and the prevalence rate of depressive, anxiety symptoms and school life dissatisfaction were 55.2%, 18.9% and 31.1%, respectively. Adjusting on other potential confounders, the result of multivariate logistic regression showed that girls engaged in less physical activities, the OR value (95%CI) was 1.597 (1.348-1.892), while the adolescents with town census register and adolescents having six or more close friends participated in more physical activities, their OR values were 0.785 (0.661-0.931) and 0.565 (0.355-0.900), and insufficient physical activity was positively associated with depressive, anxiety and dissatisfaction of their school lives, their OR values were respectively 1.783 (1.506-2.105), 2.141 (1.664-2.755) and 1.337 (1.101-1.621). Conclusion High prevalence of physical activity deficiency appeared among adolescents in Shaoxing and was closely related to the presence of depressive, anxiety symptoms and school life dissatisfaction.

Aim To study the effects of polyamine analogue CPENSpm on the human lung cancer line A549 in cell proliferation and apoptosis.Methods MTS was used to assay the cell proliferation,chemical analysis methods were used to determine the activities of enzymes in the polyamine metabolism,HPLC was performed to assay the intracellular concentration of polyamines,Sub-G1 and DNA fragmentation assays were used to determine the cell apoptosis.Results Treating A549 lung cancer cells by CPENSpm resulted in:①cell-growth inhibition and cell apoptosis;②inhibition of ODC(key enzyme in polyamine synthetic pathway)and activation of SSAT and SMO(key enzymes in polyamine catabolism);③great decrease of intracellular polyamine concentrations.MDL72527,the SMO inhibitor,can antagonize the effect of CPENSpm on inhibiting the proliferation of A549 cells.Conclusion CPENSpm inhibits proliferation and induces apoptosis of human A549 lung cancer cell line by interfering the polyamine metabolism,depleting intracellular polyamine contents that are need by quick-growth of cancer cells and inducing production of H2O2.

Objective To investigate the effects of acupuncture combined with conventional treatment on swallowing function and hemorheology in patients with pseudobulbar palsy following stroke. Methods One hundred thirty-one patients with laughing sickness after cerebral stroke from March 2010 to November 2013 were selected and divided into two group by random number table method:a treatment group (66 patients) and a control group (65 patients). The control group was treated with aspirin and rosuvastatin, while the treatment group was additional treated with acupuncture for benefiting vital energy and eliminating phlegm for 4 weeks. The swallowing function was evaluated by Kubota Water Swallow Test and the hemorheological measurements were performed. Results The total improvement rate of swallowing function in the treatment group was significantly higher than that in the control group (86.36% vs. 64.61%; χ2=8.391, P=0.004). After the treatment, the whole blood viscosities at high shear rate (4.96 ± 0.53 mPa?s vs. 5.32 ± 0.63 mPa?s;t=3.541, P=0.001) and low shear rate (23.23 ± 0.94 mPa?s vs. 23.81 ± 1.01 mPa?s;t=3.403, P=0.001), and the plasma viscosity (1.52 ± 0.24 mPa?s vs. 1.61 ± 0.28 mPa?s;t=1.976, P=0.050) in the treatment group were significantly lower than those in the control group. Conclusion Acupuncture combined with conventional treatment can improve the swallowing function and the hemorheological parameters in patients with pseudobulbar palsy following stroke.

Objective: To explore the effects of hydrogen sulfide (H 2S) on hypoxic pulmonary vascular smooth muscle cell (VSMC) apoptosis in rats. Methods: Twenty four Wistar rats were divided into 3 groups: control group ( n =8), hypoxia group ( n =8), and hypoxia +NaHS group ( n =8). The plasma level of H 2S was determined by methylene blue spectrophotometric method. VSMC apoptosis was measured by terminal deoxynucleotidyl transferase biotin nick end labeling (TUNEL). The protein expressions of Bcl 2, Fas and caspase 3 in pulmonary arteries were detected by immunohistochemical technique. Results: Compared with rats in the control group, the plasma level of H 2S decreased by 36% in rats of hypoxic group . The apoptotic rate per area in VSMCs detected with TUNEL was significantly decreased by 52.9% in rats of hypoxic group . The expressing integral score of Bcl 2 of VSMCs was increased by 123.9%,while Fas protein expression of VSMCs was decreased by 45% and caspase 3 protein expression of VSMCs was not significantly changed in rats of hypoxia group. But compared with rats in the hypoxia group, the plasma level of H 2S increased by 65% in rats of hypoxia+NaHS group. The apoptotic rate in VSMCs of TUNEL was significantly increased by 62.5% in rats of hypoxia+NaHS group. The Bcl 2 protein expression of VSMCs was decreased by 36.4% in rats of hypoxia+NaHS group. The expressing integral scores of Fas and caspase 3 were significantly higher in rats of hypoxia+NaHS group than in those of hypoxia group. Conclusion: Hypoxia decreased the pulmonary artery smooth muscle cell apoptosis. H 2S inhibited Bcl 2 protein expression of VSMCs and activated Fas and caspase-3 protein expressions of VSMCs, and therefore promoted the pulmonary artery smooth muscle cell apoptosis.

Objective To investigate the effects of dextran sulfate on lung ischemia-reperfusion injury after lung transplantation in rats.Method A total of 32 male Wistar rats were subjected to unilateral left lung orthotopic transplantation.They were randomly divided two groups (n =16 each):DXS group [DXS (10 mg/kg) was given prior to the reperfusion],and the control group (the same volume of normal saline was given).After animals were sacrificed,the lung graft was harvested 2 h after reperfusion.Oxygenation indexes,wet/dry ratio (W/D),myeloperoxidase (MPO) activity,malondialdehyde (MDA) and endothelin 1 (ET-1) in the transplanted lung,and tumor necrosis factor a (TNF-α) and interleukin 8 (IL-8) in serum were measured.The lung injury scores were evaluated and complement deposition was observed.Result After 2-h reperfusion,compared to the control group,oxygenation indexes were improved significantly in DXS group (P＜0.05),but there were no significant differences in W/D between two groups.In DXS group,the activity of MPO was significantly reduced,and the contents of MDA and ET-1 in the lung tissue were significantly reduced as compared with the control group.DXS reduced the level of TNF-α and IL-8 markedly in serum (P ＜0.05).There was no significant difference in lung injury score between two groups (4.53 ± 0.46 vs.5.28 ±0.49,P＞0.05).Compared to the control group,DXS reduced the deposition of C3c (0.8 ±0.2vs1.5±0.3) andC6 (1.2±0.4vs.2.4±0.5) (P＜0.05).Conclusion Administration of DXS attenuated ischemia-reperfusion injury after lung transplantation by inhibiting complement deposition,and improved the oxygenation of the transplanted lung.This protection was associated with inhibition of inflammation and oxidation and endothelial cytoprotection.

AIM:To investigate the role of immunoproteasome subunit β2i in deoxycorticosterone acetate (DOCA)/salt-induced vascular inflammation in mice.METHODS:Wild-type and β2i knockout male mice were used.The right kidney was removed and DOCA pellet was subcutaneously implanted in the mice.The mice were then received 1% NaCl as drinking water for 3 weeks.The total RNA and protein were isolated from thoracic aorta 3 weeks later.The aortic tissues were fixed in formalin, embedded in paraffin and sectioned.Western blot, real-time PCR and immunohistochemistry were performed to detect the expression of β2i, macrophage marker Mac-2, NF-κB, and proinflammatory cytokines IL-1β, IL-6 and TNF-α in thoracic aorta.RESULTS:Compared with sham group, DOCA/salt treatment significantly increased the expression of β2i at mRNA and protein levels, increased the infiltration of macrophages and expression of Mac-2, and upregulated the expression of NF-κB and proinflammatory cytokines including IL-1β, IL-6 and TNF-α in wild-type group, whereas theses effects were markedly attenuated in β2i knockout mice.CONCLUSION:Immuneproteasome subunit β2i is involved in DOCA/salt-induced vascular inflammation through activation of NF-κB signaling in the mice.

Objective To investigate the effects of three different concentrations of hypertonic sodium salt (HS) resuscitation on liver injury of rats at the early stage of severe burned.Methods 104 female Sprage-Dawley (SD) rats were randomly divided into five groups: sham group (n = 8), lactated Ringer solution (LR) group (n = 24), 600, 800, 1000 mmol/L HS groups (HS600, HS800, and HS1000 groups,n = 24). Rats in LR group and HS groups were subjected to full-thickness scald with 30% total body surface area (TBSA), and then given liquid resuscitation treatment with LR and the corresponding HS. These rats were sacrificed at 2, 8 and 24 hours post injury to collect blood and liver tissue. Rats in sham group were given simulation of burns without resuscitation, which were immediately sacrificed and the specimens were harvested. The levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were detected by automatic biochemical analyzer. The levels of liver tissue malondialdehyde (MDA) and superoxide dismutase (SOD) were detected by ultraviolet spectrophotometry. The expression of liver tissue p38 mitogen-actirated protein kinase (p38MARK) was detected by Western Blot.Results Compared with sham group, the levels of ALT, AST, MDA and p38MAPK were increased, and the activities of SOD were decreased in LR group and different degrees in HS groups at each time point after injury. Compared with LR group, the levels of ALT, AST, MDA and p38MAPK were decreased and the activities of SOD were increased in different degrees with HS groups, among which HS600 group changed most significantly [ALT (U/L): 147±52 vs. 227±60 at 8 hours, 138±47 vs. 191±41 at 24 hours; AST (U/L):288±79 vs. 548±237 at 2 hours, 567±167 vs. 841±338 at 8 hours, 515±180 vs. 712±159 at 24 hours; MDA (nmol/mg): 0.287±0.036 vs. 0.395±0.041 at 2 hours, 0.298±0.030 vs. 0.392±0.018 at 8 hours, 0.278±0.033 vs. 0.422±0.036 at 24 hours; SOD (U/mg): 230±16 vs. 159±30 at 2 hours, 251±14 vs. 194±15 at 8 hours, 296±8 vs. 243±11 at 24 hours; p-p38MAPK/p38MAPK (A value): 0.778±0.040 vs. 1.065±0.066 at 2 hours, 0.791±0.046 vs. 0.967±0.041 at 8 hours, 0.733±0.027 vs. 1.020±0.043 at 24 hours; allP < 0.05]. The levels of ALT and AST in HS600 group were significantly lower than those in HS1000 group at 2 hours and in HS800 group at 8 hours. The levels of MDA and p38MAPK in HS600 group were significantly lower than those of HS800 group and HS1000 group, and the level of SOD in HS600 group was significantly higher than that in HS800 group and HS1000 group at each time point after injury. There were no significant differences in all test indicators between HS800 group and HS1000 group at each time point after injury.Conclusions High concentration of HS can reduce the early liver injury in severely scalded rats, of which the curative effect of HS 600 mmol/L is best.