Internet-based tendering makes equipment purchase convenient, rapid, effective, economical and fair. The issues that should be paid attention to are also described.

Objective To evaluate the efficacy of remifentanil combined with propofol in children of different age undergoing short-term ophthalmologic operation.Methods Forty-eight children aged 4-12 yr undergoing elective short-term ophthalmologic operation were divided into 4 groups(n=12 each)according to age and anesthetics:group ⅠPK aged 4-7 yr and group ⅡPK aged 8-12 yr received ketamine and propofol combined anesthesia;group ⅠPR aged 4-7 yr and group ⅡPR aged 8-12 yr received remifentanil and propefol combined anesthesia.In group ⅠPK and ⅡPK the anesthesia was induced with intravenous bolus of ketamine 2 ms/kg and ketamine 1-2 ms/kg.In group ⅠPR and ⅡPR the anesthesia was induced with intravenous remifentanil 1 μg/kg and respiratory rate(RR),cerebral state index(CSI)and SpO2 were recorded before anesthesia induction(baseline),2 min after induction,at the initiation of the operation,15 min after the initiation of operation and at emergence from anesthesia.Adverse effects during and after the operation,induction time,analepsia time,conscious recovery time and total sedation time were recorded.The mean infusion rate of remifentanil was calculated.Results Compared with the baesline,SP,DP,HR,RR and CSI were significantly decreased in 4 groups(P＜0.05).RR,CSI and incidence rates of adverse effects were significantly lower,and the emergence time and the recovery time of consciousness were significantly shorter in group ⅠPR and ⅡPR than in group ⅠPK and ⅡPK(P＜0.05).The average infusion rate of remifentanil was lower in group ⅡPR than in group ⅠPR(P＜0.05).Conclusion Remifentanil combined with propofol can produce good anesthetic effect in children undergoing short-term ophthalmologic operation,with less adverse effects,stable hemodynamics and rapid emergence from anesthesia.The dosage of remifentanil is difierent in children at different ages.

Objective:To investigate the mRNA expression of β_2-adrenergic receptor(β_2-AR) and the receptor post signaling materials cyclic AMP(cAMP) and cAMP-dependent protein kinase (PKA) activity in myocardial infarction(MI)rats.Methods:MI model was made by ligation of rats'left anterior descending coronary artery.A total of 48 adult Wistar rats were randomly divided into four groups:Control group,the rats were sham operated,and 2 weeks post MI group,4 weeks post MI group and 8 weeks post MI group.The myocytes suspensions were untreated or treated in each group by salbutamol,isoproterenol,ICI118,551,atenolol,propranolol and pertussis toxin(PTX)respectively.The mRNA expression of β_2-AR and β_1-AR from left ventricular myocardium were detected by RT-PCR,the concentration of cAMP was determined by competitive enzyme immunoassay and the activity of PKA was determined by non-radioactive protein kinase assay.Results:Compared with Control group,the mRNA expression of β_1-AR was lower in MI groups(P<0.05),and the ratio of β_2-AR to β-AR increased from 19% to 38%(P<0.01)after MI.Compared with the untreated cells,cAMP concentration was increased by salbutamol treatment in Control group and 2 weeks,4 weeks,8 weeks post MI group by 98.1%,133.6%,147.7% and 150.7% respectively(P<0.05).Compared with isoproterenol treatment,ICI118,551 decreased cAMP concentration in 8 weeks post MI group(P<0.05).Atenolol decreased cAMP in Control group,2 weeks and 4 weeks post MI groups(P<0.05);Propranolol decreased cAMP concentration in Control and three MI groups(P<0.05).β-receptor inhibitor inhibited PKA activity in 8 weeks post MI group(P<0.05),Atenolol inhibited PKA activity in Control group and 2 weeks post MI group(P<0.05),Propranolol inhibited PKA activity in Control group and three MI groups(P<0.05,respectively).Conclusion:The ratio of β_2-AR to β-AR increased after MI.β_2-AR agonist increased cAMP concentration and PKA activity in myocytes in post MI rats.

Objective To study the telomerase activity in the tissue adjacent to bladder cancer and to investigate its clinical significance. Methods Telomerase activity was detected with telomeric repeat amplification PCR (TRAP) assay. The telomerase activity in the tissue adjacent to bladder cancer was evaluated. Results Telomerase activity was positive in the tissue samples adjacent to bladder cancer in 10 of the 24 cases(42%). Telomerase activity in the adjacent tissue has been related with the tumour grades and stages. The tumour recurrence was also related with the telomerase activity in the adjacent tissue. Conclusions The detection of telomerase activity in the tissue adjacent to bladder cancer could be a prognostic marker for bladder tumor recurrence.

Objective To investigate the expression of Shh, Ptch, Smo and Gli1 proteins who belongs to components of Hedgehog signaling pathway, and to explore the relationship between the abnormal activated of Hedgehog signaling path?way with renal clear cell carcinoma (RCCC). Methods Protein expressions were examined by immunohistochemistry in 76 cases of human RCCC and 12 pericarcinomatous tissues. And 10 fresh specimens from cancer tissues and pericarcinoma?tous tissues were used to examine expressions of Shh, Ptch, Smo and Gli1 by Western blotting. Results The expressions of examined proteins were higher in RCCC than that in pericarcinomatous tissue. Shh (52.6%vs 8.3%), Ptch (63.2%vs 16.7%), Smo (44.7%vs 8.3%) and Gli1 (60.5%vs 25.0%). The increased proteins levels were disassociated with age, gender, and tu?morous size in RCCC. But it was significantly correlated with TNM stage of RCCC. The expressions were gradually increased with higher TNM stage of RCCC or more invasive malignancy. Conclusion Hedgehog signaling pathway is highly activated in RCCC. It might be involved in initiation, progression of RCCC, and it might be used as the target for RCCC treatment.

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) are conveniently cultured and separated in vitro because theirimmunogenicity is low. Therefore, BMSCs are suitable for cell transplantation. Research has shown that BMSCs are potential to repair neurological defect. OBJECTIVE: To determine whether in vitro cultured BMSCs can be transplanted to repair peripheral nerve injury or not, and to investigate its mechanisms. DESIGN, TIME AND SETTING: Randomized controlled animal study This study was performed in Department of Toxicology, Public Health College of Jilin University from March 2006 to March 2007.MATERIALS: Fifty healthy female Wistar rats aging 2 months and six 1-week-old female Wistar rats were used for extraction of BMSCs. Rabbit-anti-nerve growth factor (NGF) monoclonal antibody was provided by Santa Cruz Company. METHODS:BMSCs were separated and cultured with adherent method. In the 3rd generation, BMSCs were preiabeled with bromodeoxyuridine (BrdU) 48 hours before transplantation. Fifty healthy Wistar rats were selected to prepare sciatic nerve crush injury models with clamping method.Subsequently, rats were randomly divided into transplantation group and control group, with 25 rats in each group. Rats in the transplantation group underwent transplantation of BrdU-labeied BMSCs at nerve injured sites; while, the same volume DMEM was injected into rats in the control group. MAIN OUTCOME MEASURES: Injured nerve in the transplantation group suffered from anti-BrdU staining 1, 2, 4, and 6 weeks after surgery. Distal injured nerve in both groups suffered from NGF immunohistochemical staining 1, 2, 4, and 6 weeks after surgery. Image analysis system was adopted to analyze integrated absorbance of positive expression. Gait analysis was performed every week after surgery to measure sciatic nerve function index, and it was also adopted to measure regenerated nerve conduction velocity 6 weeks after surgery. Subsequently, amount and inner diameter of medullated nerve fibers were calculated after luxol fast blue staining, while wet weight of experimental-lateral gastrocnemius muscle and cross section area of muscle fiber were measured at the same time. RESULTS: Fifty rats were included in the final analysis. BrdU-labeled positive cells could be found at injured nerve in the transplantation group 1, 2, and 4 weeks after surgery. Integrated absorbance of NGF protein expression in the transplantation group was significantly higher than that in the control group 1 and 2 weeks after surgery (P < 0.01), but there were no significant differences between the two groups 4 and 6 weeks after surgery (P > 0.05). Sciatic nerve function index in the transplantation group superiorly recovered to that in the control group 3-6 weeks after surgery. Furthermore, 6 weeks after surgery, nerve conduction velocity, amount and diameter of medullated nerve fibers, wet weight and cross section area of gastrocnemius muscle in the transplantation group were significantly higher than those in the control group (P < 0.05-0.01). CONCLUSION: BMSCs can be transplantated into injuried nerve tissue, and promote the recovery of nerve function in the micro-enviroment, improve NGF expression in an early phase may be one of its mechanisms.

BACKGROUND: Previous researches have proved that both atrial natriuretic polypeptide (ANP) and 5-hydroxytryptamine (5-HT) exist in the same endocrine granule of enterochromaffin cell (EC). However, whether ANP may promote or inhibit synthesis and secretion of 5-HT needs to be further studied. OBJECTIVE: To investigate the effect of ANP on synthesis and secretion of 5-HT in EC of rat gastric mucosa.DESIGN: Randomized controlled animals study.SETTING: Immunology Laboratory, Chengde Medical College. MATERIALS: This study was performed at the Immunology Laboratory, Chengde Medical College from October 2004 to July 2007. Forty adult male Wistar rats were provided by the Experimental Animal Center of Chengde Medical College. The experiment was in accordance with animal ethics standards. ANP, 5-HT antibody and serum were provided by Santa Cruz Biotechnology Company, USA.METHODS: Forty rats were randomly into endocrine and exocrine groups, and rats in the two groups were sub-grouped into control and experimental groups with 10 in each group. ANP (28 μg, 14 mg/L) was directly injected into the stomach to mimic ANP luminal secretion and ANP (14 μg, 14 mg/L) was directly injected into the sublingual vein to mimic ANP endocrinal secretion. In above condition, 5-HT immunoreactive positive cell was displayed by using immunohistochemistry technique, numerical density (Nv) of endocrine granule (SG) was counted by using electron microscopic morphometry, and 5-HT level in the serum was measured by using HPLC-ECD technique. And then, the results were compared to the control group. MAIN OUTCOME MEASURES: Effects of ANP on density of 5-HT immunoreactive positive cell, numerical density (Nv) of SG and 5-HT level in the serum. RESULTS: Effect of luminal and endocrine ANP on the 5-HT secretion: The density of immunoreactive positive cell and the numerical density (Nv) of SG were significantly increased by luminal and endocrine ANP (P＜0.05), while 5-HT level in serum was significantly reduced by luminal and endocrine ANP (P＜0.05). CONCLUSION: Luminal and endocrinal ANP can inhibit 5-HT release of gastric mucosa but may not change or enhancement its synthesis in rat.

Objective To investigate the clinical status of post-core crown in anterior teeth aesthetical restoration and to observe its effect. Methods 126 cases of anterior teeth in patients with mild deformity,restored with post-core crown after informed consent meanwhile conducted clinical follow-up observation. Results In addition to 2 cases of disease after root canal treatment, 1 incisal and labial surface of porcelain fracture, other cases achieved desired results in the way of self-satisfaction,clinical efficacy and x-ray observation and periapical films. Conclusion Post-core crown in anterior aesthetical restoration has a unique position and clinical efficacy.

Objective:To investigate the effect of potassium sodium hydrogen citrate in the treatment of renal uric acid calculi with heavy load.Methods:The clinical data of 6 patients with intrarenal high-load uric acid calculi (long diameter >4 cm) treated with potassium sodium hydrogen citrate in our hospital from January 2018 to July 2020 were reviewed. All of the patients were male. Their ages ranged from 42 to 66 years, with an average age of 51.3 years old. The average length to diameter was 6.0(4.1-7.6) cm. The average density of stone was 475 (418-535) HU. The average blood uric acid was 453.3(258.7-570.0)μmol/L, and all patients had a urine pH ≤5.5. The blood uric acid level was higher than normal serum uric acid level before treatment, and 3 cases had a history of gout. Stone composition analysis revealed 100% uric acid stone in two patients before treatment. The remaining patients were more likely to have uric acid calculi before treatment. None of the patients had a history of urinary tract infection. All patients were treated with oral potassium sodium hydrogen citrate. During the treatment, the starting dose was 10g/ day, which was divided into 2.5 g after breakfast, 2.5 g after lunch and 5.0 g after dinner. The dose was adjusted according to the pH value of urine, and the urine pH was maintained between 6.5-7.0. CT plain scan was repeated every 2-3 months during the treatment period to evaluate the treatment effect.Result:After 2.5-8.0 months’ treatment, the stone load of all the patients was reduced to different degrees, and the average length diameter was shortened by 3.2cm, among which two patients’ stones disappeared. CT scan showed the stone edge changed from smooth to rough after the treatment, and there would be worm erosion defect and cavity on the surface and inside of the stone, showing obvious stone dissolution phenomenon.Conclusions:Potassium sodium hydrogen citrate has a good therapeutic effect on renal uric acid calculi with heavy load. Non-infected patients with uric acid calculi should be treated with potassium sodium hydrogen citrate.

Objective To investigate the distribution of breast invasive ductal carcinoma with different molecular subtypes ,and to study the relationship between molecular subtypes and clinical pathological features in Xinjiang area .Methods A total of 621 patients with IDC from January 2013 to January 2014 was classified into Luminal A type,Luminal B type,HER-2 overexpression type and Basal -like type,different molecular subtypes according to the estrogen receptor(ER),progesterone receptor(PR),epidermal growth factor receptor -2(HER-2) and Ki-67,and compared the statistics combining with the clinical pathological characteristics of IDC .Re-sults The result showed that Luminal B type accounted for 53.1%,Luminal A type,HER-2 overexpression type and Basal-like type accounted for 14.5%,15.9%,16.4%,respectively.There were significant differences between the molecular subtypes and tumor size ,histological grade ,tumor pathological stage ,the expression of ER , PR and HER-2(P<0.05 for all).There were no correlations between the molecular subtypes and age ,as well as lymph node metastasis(P>0.05).Conclusion There is a close relationship between invasive ductal carcino-ma of different molecular subtypes and clinical pathological characteristics .The molecular subtypes are beneficial to treatment guidelines for clinical individual .