Objective To build rat models and observe changes of behavior and monoamine of PDD. Methods The rats were injected with 6-OHDA and accepted CUMS for the establishment of PDD model , so as to assess its behavior changes and detect brain tissue monoamine contents by ELISA. Results Significant behavioral and monoamine abnormalities can be observed in model rats. Behavioral and monoamine improvement can be observed after treated with Chaigan Jieyou. Conclusion The PDD model can be established by 6-OHDA and CUMS. Chaigan Jieyou decoction may reach therapeutic purposes by increasing monoamine contents.

Objective To explore the neuroprotection of Pabing Formula I Granules for Parkinson's disease (PD) rats. Methods PD rats were induced with injection of 6-hydroxy dopamine twice stereotaxically into the left striatum. 40 rats modeled successfully were divided into model group (n=12), high dose group (n=14) and low dose group (n=14), and the other 8 rats were as normal group. The high dose group and low dose group received Pabing Formula I Granules 18 g/kg and 9 g/kg respectively, while the model group and normal group were given distilled water at the same volume, once a day for 32 d. Then, they were assessed with rotation test. The activities of superoxide dismutase (SOD), the levels of glutathione (GSH) and malonaldehyde (MDA) of their left striatum were measured, and the expression of P-Akt (ser 473), Bcl-2, and Bax in substantia nigra were detected with immunohistochemistry. Results The rotation released was significant different among the model, high dose and the low dose groups after treatment (P<0.01), with the activities of SOD (P<0.01) and the content of GSH (P<0.01) increasing and the content of MDA (P<0.01) descreasing. There was significant difference in the content of GSH (P<0.01) and MDA (P<0.01) between the high and the low dose groups. There was significant difference in expression of P-Akt (ser 473), Bcl-2 and Bax among the model, high dose and the low dose groups, as well as the Bcl-2/Bad ratio (P<0.05). Conclusion Pabing Formula I Granules plays neuroprotective effect through enhancing antioxidation and eliminating free radicals ability and Akt signaling pathway, which appears as dose-effect relationship.

Objective To evaluate the clinical efficacy and safety of Chaigan Jieyou decoction in treating Parkinson's disease depression (PDD).Methods Applying the method of simple random contrast,this research collects 70 patients who meet the study criteria and divide the patients into two groups of 35 peoples each:Chaigan Jieyou decoction group,which received treatment of Chaigan Jieyou decoction and fluoxetine group with treatment of fluoxetine.Treatment period was 2 months.Apply the HAMD-17 (Hamilton Depression Scale),Beck Depression Scale (Beck Depression Inventory,BDI),Parkinson's composite score (unified Parkinson disease rating scale,UPDRS) and TCM symptoms rating scale to evaluate the clinical efficacy before and after treatment.Resuits Compared the two groups by HAMD Score reduction rate,there is no statistical significance in the general efficacy rate (P ＞ 0.05);compared by the curative effect of TCM syndrome,Chaigan Jieyou decoction group is better than fluoxetine group (P ＜ 0.05).There is statistical significance in the differences of all the HAMD,BDI,UPDRS Ⅱ,UPDRS Ⅲ scores between the two groups after the treatment (P ＜ 0.01,P ＜ 0.05).Compared the treatment efficacy by the HAMD scores,Chaigan Jieyou decoction can alleviate the clinical symptoms of patients of varying degrees.Conclusions Chaigan Jieyou decoction can significantly relieve depression of PDD patients,and there is exact curative effect to the mild and moderate PDD patients,improving their quality of life to a certain extent.