Objective To explore the clinical effect of combination therapy of Iamivudine and Yigan decoction in treatment of chronic hepatitis B.Methods 52 patients with chronic hepatitis B were selected and divided into control group and combination group randomly according to the digital table.Based on regular treatments,control group received treatment of lamivudine,and based on treatment of control group,combination group received treatment of Yigan decoction.Treatment efficacy,viral serological response rate,viral nucleic acid response rate and cellular immunity status were observed and compared.Results Compared with control group,treatment efficacy increased significantly from 73.08％ to 84.62％ (P ＜ 0.05) ; viral serological response rate and viral nucleic acid response rate increased significantly at the same time,from 23.08％ to 65.38％ and from 38.46％ to 88.46％ respectively (P ＜0.05) ;Composition of CD4+ T cells increased significantly in all patients after treatment,but more significantly in combination group (P ＜ 0.05) ; Composition of CD8+ T cells decreased significantly in combination group after treatment (P ＜0.05) ; ratio of CD4+/CD8+ increased significantly after treatment in all patients but more significantly in combination group (P ＜ 0.05).Conclusion Combination therapy of lamivudine and Yigan decoction is an ideal treatment of chronic hepatitis B,which can improv viral serological response,viral nucleic acid response and cellular immunity status,better than application of lamivudine alone.

Objective To explore the influence of combination therapy of IFN and lamivudine on IL-8 level in chronic hepatitis B patients.Methods 58 patients with chronic hepatitis B were selected and divided into control group and experiment group randomly and evenly.Based on regular treatments,control group received treatment of lamivudine and experiment group received combination treatment of lamivudine and IFN alpha-1 b.Restoring rate of ALT,serum conversion rate of HBeAg/anti-HBe,conversion rate of HBV-DNA and mutation rate of YMDD were observed and compared between two groups.Serum level of IL-8 was observed before and after treatment in two groups.Results Compared with control group,serum conversion rate of HBeAg/anti-HBe significantly increased,mutation rate of YMDD significantly decreased in experiment group(P ＜ 0.05).Serum level of IL-8 in two groups significantly decreased after treatment,but more significant in experiment group compared with control group (P ＜ 0.05).Conclusion Combination therapy of lamivudine and IFN shows positive effects to chronic hepatitis B,mainly via inhibiting activities of HBV and decreasing status of inflammation.

Objective To investigate the effects of propofol on myocardial nuclear factor kappaB(NF-κB)and inducible nitric oxide synthase (iNOS) during myocardial ischemia-reperfusion (I/R) in isolated rat hearts.Methods Twenty-four adult SD rats of both sexes weighing 200-300 g were randomly divided into 3 groups (n=8 each):group Ⅰ control (C) ; group Ⅱ I/R and group Ⅲ propofol + I/R (P).The animals were anesthetized with intrxperitoneal urethane 1.0 g/kg and their hearts were excised.The aorta was cannulated and the hearts were retrogradely pedused with oxygenated K-H solution in a Langandorff apparatus for 20 min.In group C the hearts were continuously perfused with K-H solution for 110 min.In I/R group after 20 min stabilization the hearts were made globally ischemic for 30 min followed by 60 rain reperfusion.In group P the hearts were peffused with K-H solution containing 50 μmol/L propofol for 20 min,the hearts were then made globally ischemic for 30 min followed by 60 min repedusinn with K-H solution containing 50 μmol/L propofol.Cardiac troponinI (cTnI) concentration in coronary effluent was measured at the end of 20 min stabilization before myocardial isobemia (baseline) and at 10 min(T1) and 60 min (T2) of reperfusion.Myocardial specimen was obtained frem left ventricle at 60 min of repeffusion for determination of MDA content,SOD and iNOS activity,the expression of NF-κB and IκB (by immuno-histochemistry).Results The cTnI concentration in coronary effluent was significantly higher at T1 and T2 in group I/R and at T2 in group P than in group C.The NF-κB expression was significantly higher while IκB expression was lower in I/R and P group than in group C.iNOS activity was higher in I/R group than in control group but there was no significant difference in iNOS between group P and C.Propofol administered before and after myocardial ischemia significantly attenuated I/R-induced changes listed above.Conclusion Propofol can attenuate myocardial ischemia-reperfusien injury through reducing the NF-κB activation and suppressing the iNOS activity.

In this paper,based on the present situation of the pharmaceutical education in China,the author brings forward the challenge and opportunity to pharmaceutical education at medical colleges,and then gives us some helpful ideas to solve the problems.

Procalcitonin ( PCT) has closely related to the severity of senile pulmonary infection, and its dynamic detection can be used as the auxiliary diagnosis index for elderly severe pneumonia ( SP) . The effects of serum PCT dynamic detection in the early quick identification, diagnosis, disease course monitoring and prognosis evaluation for elderly SP were reviewed, and the effects and clinical significance of the detection in the optimization of antibiotic therapy were also summarized in the paper. The results showed the impor-tant value of PCT dynamic detection in the diagnosis of elderly SP and anti-infection therapy.

In recent years,Wnt/?-catenin signaling has been identified as a key player in embryogenesis and human diseases.Canonical Wnt signaling pathway is controlled by a variety of classic molecules like Wnt,?-catenin,Axin,APC,GSK-3? and CK1,which interact and coordinate to regulate the expressions of cell signaling molecules.The latest evidences suggest that some components of the Wnt/?-catenin signaling,like APC,GSK-3?,CK1,Dkk2 and WISE,play dual roles different from what they have been thought previously.Here we reviewed some recent discoveries on the canonical Wnt/?-catenin signaling pathway to provide some new ideas and principles for signaling transduction studies.

Objective To explore proper methods of and environment for people oriented management so as to meet the various new challenges confronting hospitals. Methods The means of retrospection plus the Delphi method and sociological investigations were adopted. Results An exploration was made into the theories and principles to be followed, the means to be adopted and the human environment to be created in implementing people oriented management. Conclusion Hospitals implementing people oriented management and thus achieving maximized benefits in human resources management will be able to enhance their market shares and maintain sustainable development.

Objective To discuss the effect of Cordyceps sinensis liquid on Diabetic Nephropathy renal tubular epithelial cell transdifferentiation.Methods Diabetic Nephropathy renal tubular epithelial cells were treated with different dose of Cordyceps sinensis liquid and high glucose,and divided into five groups.Control group was added 5.5 mmol/L D-glucose,High glucose group was added 30 mmol/L D-glucose, Experimental group 1 was added 30 mmol/L D-glucose and 5μg/mL Cordyceps sinensis liquid,Experimental group 2 was added 30 mmol/L D-glucose and 10 μg/mL Cordyceps sinensis liquid,Experimental group 3 was added 30 mmol/L D-glucose and 20 μg/mL Cordyceps sinensis liquid.The expression of E-cadherin protein,FN protein,α-SMA protein and ILK protein in each group were detected by western blot. Results Compared with control group,the expression of E-cadherin protein in high glucose was decreased significantly(P<0.01),and with the addition of Cordyceps bacteria increases,E-cadherin protein expression in three Experiment group were gradually increased(P<0.01).Compared with control group,the expression ofα-SMA protein and FN protein in High glucose group were increased significantly (P <0.01 ),and both two protein in Experiment group 2 and Experiment 3 were significantly lower than High glucose group(P<0.01).Compared with control group,the expression of ILK protein in high glucose group was significantly higher(P<0.01 ),and its expression in Experiment group 3 was significantly lower than High glucose group (P<0.01 ). Conclusion Cordyceps sinensis liquid can inhibit diabetic nephropathy renal tubular epithelial cell translating into myofibroblast,then inhibit renal tubule interstitial fibrosis.

Objective To explore the status of college students’ attachment and locus of control, and explore their relationship. Methods A total of 198 college students were surveyed adopting Experiences in Close Relationships Inventory and Internality, Powerful Others and Chance Questionnaire. Results The distribution of college student′s attachment type was not balanced,the proportion of dismissing type,security type, fearful type, preoccupied type accounting for 43. 9%,31. 3%,21. 7%,3. 1% respectively. The scores of internality, chance and powerful others were (27. 59 ± 5. 44),(17. 92 ± 6. 66),(17. 70 ± 7. 32) respectively. Attachment avoidance correlated negatively with internality(r= -0. 259,P<0. 01), and positively with chance and powerful others(r=0. 206,0. 221,P<0. 01);besides there was a positive correlation between attachment anxiety and chance,powerful others(r=0. 378, 0. 410,P<0. 01). Attachment avoidance had prediction on internality(R2 =0. 067,P<0. 01). Attachment anxiety had prediction on chance and powerful others(R2 =0. 143,0. 168,P<0. 01). Conclusion College students’ at-tachment has significant effects on locus of control.

Objective To investigate the effect of different methods of intestinal preparation on intestinal cleaning for elderly gynecological patients undergoing surgery. Methods Sixty-four elderly patients undergoing gynecological surgery were divided into two groups according to admission seguence. The control group was treated with enema using 800~1000 mL 0.2%soapy water, while the experiment group was treated with enema using sodium phosphate salt oral solution mixed with 800 ~ 1000 mL 0.9% sodium chloride solution. The intestinal cleanness and enema times of the two groups were observed. Results The effect of intestinal cleanliness in the experiment group was better than that in the control group. There was significant differences between the two groups. (P<0.01). Conclusions For the elderly patients undergoing gynecological surgery, sodium phosphate salt oral solution can not only reduce the frequency of enema but also achieve the purpose of cleaning intestinal tract, to ensure the operation effect. It is the ideal method for cleaning bowels of the elderly patients in preoperative prepartion of the surgery.