OBJECTIVE To regulate the task of washing,disinfection and sterilization of stomatological apparatus for diagnosis and to prevent and control nosocomial infection.METHODS The departments of stomatology of 8 manicipal hospitals and 11 county hospitals were examinated comprehensively to find out the problems in washing,disinfection and sterilization of stomatological apparatus.Then the improve measures were proposed.RESULTS The washing,disinfection and sterilization were not eligibles,manipulations not standardized,and lack of knowledge about nosocomial infection hazards in some county hospitals.CONCLUSIONS It should be strengthen the apparatus washing,disinfection and sterilization to prevent and control,the nosocomial infection.

It is very important for pulmonary disease treatment to monitor the pulmonary function continuously. In this paper, the hardware of electrical impedance tomography (EIT) system is designed, and simulative model of human thorax is established for image reconstruction based on Landweber pre-iteration method. Compared with linear back projection, the method above results in the better image reconstruction with little trailing and increased speed of real-time reconstruction.

Objective To confirm the risk factors of poor prognosis in patients with severe acute epiglottitis by comparing symptoms and results of laboratory tests.Methods A total of 698 patients with acute epiglottitis from outpatient and emergency room from 1995 to 2014 were retrospectively studied.These patients were divided into severe or mild group as per the means of treatment of airway including invasive (n =115)and non-invasive (n =583).The past history,general conditions and laboratory tests were compared between the two groups by chi-square or t test;the spearman correlation between the degree of dyspnea and epiglottis edema was analyzed,and the risk factors of poor prognosis were detected by logistic regression.Results The ratio of male to female was 1.366/1.There was a high prevalence of sever acute epiglottitis in spring,winter and at night, respectively.More smokers were found in the severe group compared with the mild group (χ2 =41.957,P <0.01).Severe dyspnea and low PaO2 (r =0.573,P <0.01),but not the poor grading of epiglottis edema evaluated by pharyngo-fiberoscope (r =-0.024,P =0.525),were correlated with poor prognosis.Male (OR =1.84,95%CI:1.41 -3.22,P =0.001),an attack at night (OR =2.61,95%CI:1.98 -3.16),P =0.07), smoker (OR =1.63,95%CI:1.05 -3.39,P =0.04)and low PaO2 (OR =2.97,95%CI:1.58 -4.49,P =0.02)were independent risk factors for a poor prognosis.Conclusions Male,an attack at night,smoker and low PaO2 were independent risk factors for a poor prognosis of acute epiglottitis.A critical care should be given to patients with those risk factors,even their epiglottis edema was not very serious.

Objective: To study the inhibitory effect of VEGF antisense oligonucleotides (ASODN) on growth of Lewis lung cancer in C57BL/6 mice.Methods: Lewis lung cancer cells were cultured and implanted subcutaneously into 40 C57BL/6 mice, which were then divided into 4 groups: VEGF-ASODN treatment group, VEGF-SODN treatment group, BEGF-MODN treatment,and control group (normal saline). Mice in different groups were treated 24 hours after cell inoculation. The weight and volume of subcutaneous tumors was measured and the morphological changes of tumor cells was observed under microscope. VEGF protein and microvessel density were examined by immunohistochemistry.Partial tumor tissues were kept in liguid nitrogon.Results: The average tumor weights of the control, VEGF-ASODN, VEGF-SODN and VEGF-MODN groups were (7.33?0.71)g, (4.56?0.38) g, (7.59?0.32) g, and (7.62?0.39) g, respectively. The inhibition rates of tumor growth in VEGF-ASODN, VEGF-SODN and VEGF-MODN group were 43.8%, 5.5% and 3.1%, respectively. VEGF-ASPODN obviously inhibited the tumor cell growth and decelerated the tumor cell proliferation. Immunohistochemistry results showed that the expression of VEGF in ASODN group was remarkly lower than those in SODN group, MODN group and control group (P

AIM: To investigate the effect of adrenomedullin on the fibrogenic action of TGF? 1 and the cellular mechanism in renal tubular epithelial cell line (HK-2). METHODS: Total collagen synthesis and secretion were reflected by -proline incorporation and the radioactivity of -hydroproline in the culture medium; ELISA method was used to detect the level of secreted fibronectin (FN); the expression of FN mRNA, collagen IV mRNA and TIMP-1mRNA were determined by RT-PCR; western blot was used to detect the expression of Smad2 and Smad6 proteins. RESULTS: (1) AM could antagonize the expression of collagen and FN stimulated by TGF? 1 in both transcription and translation level; (2) AM down-regulated the TGF? 1-induced TIMP-1 mRNA expression; (3) AM had no effect on the over-expression of Smad2 stimulated by TGF? 1; but could up-regulate the expression of Smad6 inhibited by TGF? 1. CONCLUSION: AM could antagonize the fibrogenic effect of TGF? 1 through up-regulating Smad6 expression in HK-2 cell line.

Objective To study the relationship between perioperative detection of forkhead box protein P 3(Foxp3) mRNA level in peripheral blood mononuclear cell (PBMC ) of patients with kidney transplantation and chronic allograft nephropathy (CAN ) . Methods 35 cases of renal allografts completed in this hospital defined clinically and confirmed histopathologically were observed as CAN group ,simultaneously a retrospective case-control study was performed on 35 renal transplant recipients with normal graft function observed(control group) .Foxp3 levels of PBMC in perioperative period were dynamically determined in two groups .Real time PCR was applied to detect the expression of Foxp3 mRNA .In order to find out other risk factors influencing chronic allograft nephropathy and eliminate disturbance .The related clinic factors were compared between the CAN group and the control group .Re-sults The Foxp3 mRNA level was lower in the CAN group than the control group [(24 .1 ± 16 .2) × 10-4 vs .(52 .3 ± 27 .4) × 10-4 ,P<0 .01] .Among risk factors influencing chronic allograft nephropathy ,only acute rejection(AR) was probably different in two groups .In order to avoid disturbance ,further classification was done according to AR .The statistical results show that the Foxp3 mRNA level in two groups was not be affected by AR .Conclusion The Foxp3 mRNA level in perioperative period is associ-ated with CAN which maybe a risk factor for CAN .Foxp3 may be an index to predict the occurrence risk of chronic allograft ne-phropathy after kidney transplantation and guide individual treatment .

Currently,with the increasing tension in doctor-patient relationship and medical disputes, it is of great importance to train medical students’ competence of establishing harmonious doctor-patient relationship. By analyzing the existing problems,the ways to train medical students’ competence in establishing harmonious doctor-patient relationship in clinical practice teaching hospital especially in oncology ward is discussed.

BACKGROUND:Osteoporosis is a condition resulting in an increased risk of skeletal fractures due to a reduction in bone mass and degradation in bone microstructure. Treatment of osteoporosis usual y involves the application of pharmacological agents as the first choice. Pharmacotherapy alone is not sufficient for the comprehensive management of osteoporosis, as pharmacotherapy has no effect on increasing muscle strength, improving balance ability, and preventing fal s. Exercise, considered as an important non-pharmacologic therapy, plays a considerable role in the prevention of osteoporosis. The importance of therapeutic exercises in the treatment of osteoporosis has been recognized gradual y.
OBJECTIVE:To discuss the effect of exercise in the treatment of osteoporosis combined with some of the latest research literatures.
METHODS:Clinical reports and mechanism researches about effects of different types of exercise on bone strength especial y the treatment of osteoporosis were retrieved from CNKI database and Medline database with the key words of“osteoporosis, bone mineral density, bone strength, treatment, exercise, stress, tai chi, muscle, bone architecture”in both Chinese and English from January 2001 to February 2013. The repetitive researches and atypical reports were eliminated.
RESULTS AND CONCLUSLON:Therapeutic exercises including aerobic exercise, resistance, impact movement, and vibration, which can safely increase bone strength and muscle strength, improve equilibrium function, and prevent fal s and fractures. For spinal deformity patients, appropriate orthotics can improve the security and promote exercise therapy. As with drug therapy, therapeutic exercises are also individualized. Exercise program should be selected under the insurance of good compliance and security. Exercises that involve high strain rates seem to be more effective than others. However, al these benefits are comparatively smal , and should be maintained by continuous exercises.

Objective To explore the application and effect of modified-type nasojejunal tube of early enteral nutrition in severe traumatic brain injury patients. Methods 60 sever brain injury patients were collected in the neurosurgery ward of our hospital. The patients were divided into control group and trial group by random number table. The patients in control group were treated with normal spiral nasojejunal tube, and the patients in trail group were given modified-type nasojejunal tube. The study mainly researched the success rate of intubation, the operation time of two groups, the recovery time of gastropareaia and so on. Results The success rate of intubation of experimental group was 93.3%(28/30) which was significantly higher than 73.3% (22/30) in the control group (χ2=4.320, P<0.05). The operation time of two groups were (22.67±1.77) minutes and (28.90±3.39) minutes. The operation time of two groups had significant difference (t=8.936, P<0.05).The recovery time of gastropareaia of two groups were (17.37 ± 8.29) days and (21.60 ± 7.82) days. The recovery time of gastropareaia of two groups had significant difference between two groups (t=2.035, P<0.05). There was no satistically significant difference of Glasgow Coma Scale (GCS) score between two groups (P>0.05). The satisfaction of patient or family of experimental group was 93.3%(28/30) which was significantly higher than 60.0%(18/30) of the control group (χ2=9.317, P<0.05). Conclusions The modified-type nasojejunal tube was conducive to improve the success rate of intubation. The symptoms of gastroparesis were recovered early. The modified-type nasojejunal tube was easy operation, suitable for beginners and young nurses. The satisfaction of patient or family was higher.

Objective:To compare the difference of human epididymis protein 4(HE4) and carbohydrate antigen 125(CA125) indexes before and after menopause in ovarian cancer patients, and to evaluate the efficacy of risk of ovarian malignancy algorithm(ROMA) index in early diagnosis.Methods:The clinical data of 236 patients with ovarian cancer (malignant ovarian tumor group) who were hospitalized in Cancer Hospital of China Medical University from April 2018 to September 2019 were retrospectively analyzed, and 312 benign patients treated at the same period were selected as the control group (benign ovarian tumor group). On the basis of clinical pathological section confirmation, the serum samples were collected to detect HE4 and CA125, and the ROMA index was calculated according to the formula.The differences of HE4, CA125 and ROMA indexes before and after menopause, as well as the diagnostic efficacy of ROC survival curve of the three were compared, and the relationship between the different clinical characteristics of ovocarcinoma patients was evaluated to comprehensively analyze the early diagnostic effect of ROMA index.Results:In the serum of patients with malignant ovarian cancer, HE4, CA125 and ROMA index were abnormally high expression, and the difference between HE4 before(263.2(128.4, 385.6) pmol/L) and after menopause(354.8(179.5, 554.2) pmol/L) was statistically significant( Z=2.09, P=0.037). Through fitting the ROC curve: the AUC of premenopausal ROMA index was 0.939, and the AUC of postmenopausal ROMA index was 0.933, which were higher than HE4 (0.937, 0.906) and CA125 (0.913, 0.924), and their corresponding Youden correct diagnostic indexes were 0.800 and 0.742 respectively, which were higher than HE4 (0.788, 0.653) and CA125 (0.498, 0.565). The two categories of HE4 were associated with the occurrence of peritoneal effusion (the percentage of postmenopausal and premenopausal HE4<140 nmol/L and premenopausal HE4<70 nmol/L were 25.77%, 74.23% and 74.23% respectively), and the difference was statistically significant (χ 2=10.155, P=0.006). CA125 was associated with the classification of the largest diameter of parenchymal tumor and the occurrence of peritoneal effusion (all P<0.05). ROMA index was associated with menopause or not, classification of maximum diameter of mass, classification of maximum diameter of parenchymal tumor and occurrence of ascites, and the differences were statistically significant (all P<0.05). Conclusion:ROMA index has a good diagnostic efficiency in the diagnosis of malignant ovarian cancer, especially premenopausal patients with good diagnostic efficiency, for early diagnosis of ovarian cancer has a certain guiding value, the follow-up will further carry out confirmatory research.