OBJECTIVE:To understand ADR and ADE reports induced by Shuanghuanglian injection in Guangxi Zhuang Na- tionality Autonomous Region of 2009 to provide reference for clinical application of Shuanghuanglian injection and ADR monitoring. METHODS:818 ADR and ADE cases induced by Shuanghuanglian injection from Jan. 1 and Dec. 31 of 2009 collected from Guangxi Center for ADR Monitoring were analyzed statistically. RESULTS:Male(n=437)involved in 818 ADR cases was more than female(n=381).Ratio of male to female was equal to 1.15∶1.The ADR incidence of patients aged from 0 to 9 was higher than other age groups,accounting for 43.77%.88.39%of primary disease in ADR patients was respiratory diseases.27.25%of cassound prognosis. Occurrence time of ADR cases were short,777 cases were collected from primary hospitals,accounting for 94.99%. CONCLUSION:Great importance should be attached to the application of Shuanghuanglian injection,ADR monitoring and rational use of drugs.

Objective To investigate the correlation between moderate hypoxia and tumor invasiveness in colon carcinoma cell line HT‐29 and detect the expression of NF‐κB protein in nucleus and activities of MMP‐2/9 ,so as to explore its latent mecha‐nism of malignant transformation .Methods The moderate hypoxia model was established according to the pressure of O2 of tumor in vivo .After different period in moderate hypoxia ,activities of MMP‐2/9 secreted by HT‐29 cells were measured by gelatin zymog‐raphy ,NF‐κB levels in nucleus were assessed by Western blot after extraction of nucleic protein .Results MMP‐2/9 activities under moderate hypoxia gradually up‐regulated in 12 h and reached the peak at 24 h .The expression of NF‐κB protein in nucleus was simi‐lar to that of MMP‐2/9 .Conclusion Moderate hypoxia could induce malignant phenotype in HT‐29 cells through up‐regulating MMP‐2/9 activities ,in which NF‐κB may involve in this progression .

Objective:To study the inflammation stress reaction between laparoscopic operation and open operation in treating hysteromyoma.Methods: hysteromyoma patients in our hospital from January 2011 to December 2012 were enrolled and randomly divided into observation group given laparoscopic operation and control group given open operation. Then inflammatory factors levels were detected, operation trauma index were observed and the correlation were analyzed.Results: (1)After treatment, inflammatory factor levels of two groups were higher than those before treatment and IL-1(t=5.892,P<0.05), TNF-α(t=11.842,P<0.05)levels of observation group were lower than control group, the difference was statistical significance(P<0.05); (2) There was no significant difference between two groups of operation time(t=0.932,P>0.05), intraoperative bleeding volume(t=7.842,P<0.05), postoperative anal exhaust time(t=7.283,P<0.05), bed time(t=8.213,P<0.05) and NRS pain scores(t=7.883,P<0.05) of observation group were lower than control group(P<0.05); (3) Intraoperative bleeding volume, postoperative anal exhaust time, bed time and NRS pain scores were positively correlated with IL-1,r2 were separately 0.485, 0.521, 0.442, 0.451; and positively correlated with TNF-α levels,r2 were separately 0.556, 0.513, 0.423, 0.458, the difference was statistical significance(P<0.05).Conclusion: Laparoscopic operation is helpful to reduce the operation wound and relieve postoperative inflammatory reaction; and there was close correlation between inflammatory stress and operation trauma.

Objective To explore the effects of interactive health education on the knowledge and behaviors of medicine use and the biochemistry indicators in hemodialysis patients. Methods Forty-eight eligible hemodialysis patients were intervened with interactive health education once every month. After three times'intervention, phosphorus, hemoglobin and hematocrit level were compared before and after the intervention. Results After the intervention, phosphorus, levels of hemoglobin and hematocrit were all significantly improved ( P<0 . 001 ) compared with that before the intervention . Conclusion The interactive health education can improve the knowledge and compliance behaviors in medicine use, serum phosphate and symptoms of anemia in hemodialysis patients.

Objective To discuss the accuracy of 3DSS three-dimensional scanning system, which was used to reconstruct the 3D digital model of unilateral orbital defect patients. Methods By using 3DSS spectrometer on the case of patients with unilateral orbital defect, the related scan dates of facial structure were collected, and the reconstructed digital models were processed wax pattern by SLS prototyping machine. Results The digital solid model of three-dimensional reconstruction was smooth and complete, and border over the natural. The designed resin prosthesis can be inosculated with the orbital defect tightly and symmetrically. Conclusion To adopt the system can be successfully completed unilateral orbital defect restoration design and production, and ultimately satisfied the clinical requirement of prosthesis production.

Objective To detect the expression of HER2 and TOPOⅡ? in human breast cancer to explore the relationship between them and the potential value in the chemotherapy of breast cancer. Method 24 breast cancer tissues and paired normal tissues were investigated. RT-PCR and imumohistochemical staining were used to detect the expressions of HER2 and TOPOⅡ? at the transcription and protein level respectively. Results HER2 mRNA expressed positively in 29% (7/24) breast cancer tissues vs 4% (1/24) in paired normal tissues. The positive expression of TOPOⅡ? mRNA in breast cancer tissues and paired normal tissues were 92% (22/24) and 21%(5/24) respectively. The result of the immunohistochemical staining showed that the positive rates of HER2 and TOPOⅡ? in breast cancer tissues were 30%(6/20) and 94%(16/17) respectively, which was consistent with RT-PCR. The 6 cancer samples with HER2 positive expression coexpressed TOPOⅡ?. The expression of these two proteins was correlated(R=0.524,P

Objective:To meet the multitier needs of medical students′ health and health career development through the establishment of relevant mechanisms for medical charity and strengthening the social responsibility theme education among medical students. Methods:The present study was conducted in a stratified sample using the method of investigation mainly. The questionnaire was self -conducted; in the end 967 valid questionnaires were collected and combined with 48 relevant interviews. The data was analyzed using SPSS l6. 0. Results: The current positive trends of social responsibility among medical students are gratifying. The students had correct atti-tude to study but the initiative needed to be improved. The professional cognition was optimistic but the morality emphasis needed to be strengthened. The capacity cognitive was clearly but the awareness of accountability needs to be improved. The social consciousness was intense but responsibility needed to be strengthened. The life value was positive but the physical and mental health needed concerns. And it was needed to further strengthen social respon-sibility education. Conclusion: We should make full use of the excellent resources of the Affiliated Hospital of Medical College. With the medical charity starting point, the social responsibility should be strengthened through the Five in One promotion mechanism that including the culture education leading, clinical practice inspiring, social practice annealing, campus culture edifying, and powers of role model effecting.

ObjectiveTo observe cluster needling of scalp acupuncture combined with rehabilitation(Tang's Approach) as an early intervention for the children with high-risk of cerebral palsy.Methods62 infants with high-risk of cerebral palsy in hospital from Jan. 2006 to Dec. 2008 were divided into the acupuncture with rehabilitation group and rehabilitation group. The scores of Mental Develop Mental Index (MDI) and Psycho-Motor Developmental Index (PDI) were assessed before and 3 months after the treatment. ResultsThe scores of MDI and PDI were better in acupuncture with rehabilitation group than in the rehabilitation group 3 months after the treatment (P＜0.01). There were significant differences in reducing dysfunction in the acupuncture with rehabilitation group compared with the rehabilitation group (P＜0.01). The follow-up result showed that there were certain advantages in reducing incidence of cerebral palsy by the acupuncture combined with rehabilitation. ConclusionAs an early intervention, Tang's Approach can improve motor function in children with high-risk cerebral palsy.

A poly(amino black 10B)/Nafion modified glassy carbon(GC) electrode for the detection of dopamine(DA) has been developed. The modified electrode showed an excellent electrocatalytic effect on the oxidation of DA. In cyclic votammetric measurements, the redox peak current increased linearly with the concentration of DA in the range of 2.0×10~(-7) 3.0×10~(-5) mol/L in PBS of pH 6.0, with a correlation coefficient of 0.9993 and 0.9991, respectively. The detection limit(S/N=3) was 1.0×10~(-7) mol/L. Moreover, the modified electrode exhibited good repeatability and long-time stability, and avoided the interference of ascorbic acid. The modified electrode has been used for the determination of DA in the injections and calf serum with good recovery and reproducibility.

Objective To study the effects of diagnostic ultrasound combined with microbubble contrast agent on augmentation thrombolysis in vivo and to discuss the synergism and mechanism of combination of urokinase. Methods Thirty-two rabbits of femoral vein thrombus were divided into four groups: simple urokinase group, ultrasound and contrast microbubble group, ultrasound and contrast microbubble combined with urokinase group, control group. Then according to the best parameter combination which was definited in previous experiment, the experiments combination of urokinase were performed. Color Doppler was used to evaluate the recanalization of different group in 15 rain,30 min and 60 min. Results After the treatment of 15 rain and 30 min,the recanalization rate in ultrasound and contrast microbubble combined with urokinase group improved significantly compared with other groups (P<0.05);after 60 min, the recanalization grade of ultrasound and contrast microbubble and urokinase group was evidently better than other two group. The result of immunohistochemistry showed that disaggregation of clot and fibrous in this group at 60 min was better than the results at 15 min, 30 min. Conclusions Diagnostic ultrasound with microbubble contrast agent showed superior effects of thrombolysis and the synergism of combination of urokinase in vivo.