Mycoplasma pneumoniae(MP) is the smallest organism that dose not need a host cell for replication.It is one of the most common pathogens of community acquired pneumonia in children.In recent years,the incidence of pneumonia mycoplasma pneumonia increased year by year,and severe pneumonia mycoplasma pneumonia,refractory pneumonia mycoplasma pneumonia cases also increased.MP causes a variety of clinical presentations,in addition to respiratory infections,there are also many extrapulmonary manifestations.Immune response plays a very important part in pathogenesis of MP infection.This article focused on the immune aspects in pathogenesis and extends to some diagnosis and therapeutic implications.

OBJECTIVE:To study the effects of 1,25-droxyvitamin D3 on nitric oxide and nitric oxide synthase in peripheral blood mononuclear cells(PBMC)in patients with spinal tuberculosis and to explore its immunoregulation function.METHODS:Patients' PBMC were assigned to test group in which DHVD3 was added or control group in which culture medium was added,then cultured after BCG vaccine inoculation.Cultured supernatant fluid from isolated PBMC was collected at 0,3,6,9,12 days respectively for measuring of relative optical density(OD)of NO by nitric oxide kit.Total RNA extracted from the PBMC was reverse transcribed to cDNA and amplificated by fluorecent real-time quantitative PCR on the sixth day;mRNA of target gene iNOS and reference gene 3-GAPD were analyzed and compared by relative quantitative method 2-??Ct.RESULTS:OD value of NO and mRNA of iNOS in test group were all higher than in control group(P

Objective To analyze the distribution and content characteristics of highly cited pa pers in Chinese nursing journals,in order to give a reference for nursing researchers to design their studies and to improve more high quality nursing papers.Methods Nursing papers were searched from database of China Academic Journal Network.Papers cited 100 times or more were collected for statistical analysis.Results There were 266 nursing papers cited 100 times or more.They were found in 9 nursing journals,of which Chinese Nursing Journal was 69.1％.The paper most highly cited was 1 298 times.The contents of highly cited papers covered basic nursing (infusion nursing and air way nursing),health education,nursing management (safety management,nursing laws,and nursing profession protection),humanistic care,psychological nursing care,and pressure of nurses.Conclusions In order to produce high quality of nursing paper,nursing researchers should improve their academic sensitivity,pay more attention to the scientific fronts,emphasize basic nursing research and study persistent.

Objective To compare the effects of intraoperative anesthetic management on im-mune function between enhanced recovery after surgery (ERAS)and traditional approach in laparo-scopic rectal surgery.Methods Clinical data of 90 laparoscopic rectal surgery in Shanxi Provincial People’s Hospital from January 2013 to January 201 5 were prospectively analyzed.90 cases (male 5 1 cases,female 39 cases,aged 48-70 years,ASA grade Ⅰ-Ⅲ)were randomly divided into two groups (n = 45 each)using a random number table:ERAS group (group A,n = 45 )and control group (group B,n = 45 ).In group A patients were treated with epidural general anesthesia,maintaining perioperative normothermia,perioperative goal-directed fluid therapy and patient controlled epidural analgesia,while in group B routine anesthetic management and patient controlled intravenous analgesia were used.The levels of blood CRP and IL-6,CD3 + ,CD4 + ,CD8 + ,CD4 +/CD8 + on the first day before operation,the first day and the third day after operation were recorded.Results The blood CRP and IL-6 levels in two groups were increased on the third day after operation(P <0.05), however the increase was more significantly in group A than in group B (P <0.05).Compared with the first day before operation,CD3 + ,CD4 + ,CD8 + and CD4 +/CD8 + levels in two groups were de-creased on the third day after operation(P <0.05),on the third day group A decreased significantly, which was more than that in group B(P <0.05).Conclusion In the relief of operative stress and the protection of immune function through enhanced recovery after surgery in anesthetic management.

Objective To construct a scientific and objective assessment to value nurse′s ability of scientific research in order to provide an objective unified standard and operable method to value nurse′s ability of scientific research and provide a standard for medical institutions to select nurses of scientific research. Methods After searching literatures and interviewing 7 experts, Delphi method was used to construct primary variables of nurse′s ability of scientific research. Then a second interview was given to each of 18 experts. The variables were weighted using analytic hierarchy process. Results Variables to assess nurse′s ability of scientific research included 2 first grade indices, 9 second grade indices and 35 third grade indices. Questionnaire return rates for two rounds of interviews were 84.00%(21/25) and 85.71%(18/21), respectively. Degree of authority Cr was 0.938. Scores of variables′importance ranged at 4.06-4.96. Ratios of full scores ranged at 33.33%-94.44%. Variable coefficients ranged at 0.05-0.25. The weight coefficients of 3 first grade indices (scientific research accumulation, explicit ability of scientific research, and intrinsic ability of scientific research) were 0.475 and 0.525, respectively. Conclusions The nurse′s ability of scientific research assessment system based on Delphi method and analytic hierarchy process is scientific and objective. This system combines quantitative and qualitative variables. It thus can calculate the ability of nurse′s scientific research according to the three grade indices. It is operable to be used to assess the ability of nurse′s scientific research.

Objective:To study the effect of different substrates coated on the cell survival and neurite outgrowth of spinal motor neurons (SMN) from embryonic rat cultured in vitro.Methods:The ventral spinal tissue was isolated from embryonic rats and digested into dissociated cell suspention for culture,then the cells were identified as SMN by immunohistochemistry stain.Poly-L-lysine(PLL) was dissolved into distilled water,phosphate-buffered saline solution,boric acid at 0.1 and 0.01 mol/L concentration respectively.The different substrates include PLL,collagen Ⅰ,laminin and PLL combined with laminin.Distilled water was used as control.The neuron survival numbers and the mean length of the neurites were measured and compared.Results:The cells on the PLL dissolved into boric acid at 0.01 mol/L concentration survived well.The SMNs grown on the PLL combined with laminin were in dispersed disitribution with high survival rate.Conclusion:PLL combined with laminin is the best for the study of the motor neuron including both soma and neurite.

Objective To investigate the changes in the concentration of myocardin in children with lupus nephritis (LN) under different degree of vessel damage.Methods Forty-nine children diagnosed with LN by routine tissue immunolfuorescence, light microscope, and electron microscope were included, and 30 healthy children were included as control group. The pathological classiifcations were performed according to the ISN/RPS 2003 LN pathological classiifcation criterion. According to the Katafuchi evaluation method, the semi quantitative assessment of glomerular and kidney tubule damage was carried out, and the degree of vascular damage was evaluated at the same time. Double antibody sandwich method was used to detect the concentration of serum myocardin.Results The glomerular and kidney tubules damage in children with LN were signiifcantly aggravated with higher pathological classification (P<0.05). Glomerular damage was positively correlated with renal interstitial damage (r=0.96, P<0.01). The degree of vascular damage was related to the degree of glomerular injury and renal interstitial injury, while it was no related with the results of clinical tests. There were different concentrations of myocardin among mild-, moderate-, severe-vessel damage and control groups (F=378.61,P<0.001), and the concentration of myocardin in moderate- and severe-vessel damage groups were obviously lower than those in control group and mild-vessel damage group (P<0.01) while there was no difference between control group and mild-vessel damage group (P>0.05). According to pathological type, there were signiifcant differences in the concentration of myocardial between control group and different pathological types (F=626.793,P<0.01). FromⅡ,Ⅲ,Ⅲ+Ⅴ,Ⅳ toⅣ+Ⅴ, the concentrations of myocardial were decreased systematically, and there were statistic differences between groups (P all<0.05).Conclusion The concentration of myocardin in children with LN can relfect the renal vascular damage to a certain extent. Elevation of myocardin concentration may be helpful for the repair of vascular damage.

Objective To study the effects of serum from a patient with acute motor axonal neuropathy (AMAN) on the sensory and motor neurons culture in vitro from embryonic rats. Methods Dorsal root ganglions and spinal ventral tissue were isolated from embryonic rats and digested into dissociated cell suspenstion for culture. The cells were identified by immunohistochemistry stain. When culturing for a week, AMAN serum was exposed in a 25% concentration, with normal human serum as control group. The AMAN serum was tested by anti-Penner O∶19 campylobacter jejuni lipopolysaccharide antibody positive. The changes of motor and sensory neurons were observed and the neurons were stained by using Guillery Shirra and Webster method which was sensitive to degeneration of nerve fiber. Results After normal serum exposure, neurons and their neurites were normal and stained in yellow color without silver granular deposition by using Guillery Shirra and Webster method. While after AMAN serum exposure, the axon from motor neuron became degenerating and stained in brown-black color increased silver-phile property. Conclusion The serum of AMAN patient might be specifically toxic to the neurites of motor neuron and might cause the degeneration of axon following soma changes. The damage of axon might be the response of campylobacter jejuni lipopolysaccharide antibody without participation of macrophage and complements.

Objective To evaluate the role of contrast enhanced CT (CECT) scanning in the diagnosis and treatment planning of some rare liver tumors. Methods We retrospectively reviewed the CECT imaging features of 10 cases with rare tumors of the liver proved by surgical pathology in 8 cases or by liver biopsy in 2 cases, with correlation of relevant clinical manifestations, laboratory results and surgical findings. Results Three cases were histopathologically proved to be benign, and 7 cases were malignant. On CT images, the liver lesions were mixed cystic solid in 5 cases, totally solid in the other 5. Eight cases demonstrated heterogeneous enhancement, while 2 cases of liver lymphoma had no enhancement. The anatomic relationship of tumors to intrahepatic vasculature, the compression and infiltration of neighboring abdominal structures were accurately delineated by CT as compared with findings at operation. Clinical manifestations and laboratory findings were not useful for the qualitative diagnosis of rare liver tumors, except for hepatocellular carcinoma. Conclusion CECT is very useful for the detection of rare liver tumors and the fine depiction of local extent of these tumors. When correlated with clinical and laboratory information, it helps to differentiate rare liver tumors from hepatocellular carcinoma. But its role in the characterization of rare liver tumors is limited.

OBJECTIVE: To establish a method for analysis of proteome of human peripheral leukocytes and to explore the difference between the healthy volunteers and patients with acute lymphocyte leukemia(ALL) in leukocyte proteomics and to study the ALL-related abnormal proteins.METHODS: Peripheral blood samples taken from 10 healthy volunteers and 10 ALL patients were separated for the extraction of leukocyte proteins.The leukocyte proteins were separated by two-dimensional electrophoresis(2-DE).2-DE patterns were analyzed by PDQuest 2D software and the differentially expressed proteins were identified by matrix assisted laser desorption ionization-time of flight-mass spectrometer(MALDITOF-MS) and bioinformatics.RESULTS: The 2-DE patterns of peripheral blood leucocytes from patients with ALL versus those from healthy volunteers were analyzed by PDQuest 2D software and the differentially expressed proteins identified by matrix assisted laser desorption ionization-time of flight-mass spectrometer(MALDI-TOF-MS) were confirmed to beALL-related proteins: AnnexinA5 and cytoskeleton 14 etc.CONCLUSION: Using MALDI-TOF-MS and bioinformatics,the AnnexinA5 and cytoskeleton 14 proteins had been proved to be of close relationship with ALL.The results provided a satisfactory basis for the searching for treatment targets and the molecular targets for the early diagnosis of ALL.