Totally 1 patient were selected from Beijing Xishan Institute for Neuroregeneration and Functional Recovery in Shijingshan District in May 2006. The patient received acupuncture treatment in trideca-ghost points after olfactory ensheathing cells (OECs) transplantation. Detailed acupuncture method and acupuncture point were as follows: Acupuncture was performed from left side on Shuigou (DU 26), Jiache (ST 6), Fengfu (DU 16), Chengjiang (RN 24), Shangxing (DU 23), oblique needling from Shuigou to mittele muschel 0.3 cun. Needle insertion on Chengjiang was from left to right, for 1 minute without retaining needle. Then acupuncture on Shaoshang (LU 11), Daling (PC 7), Jianshi (PC 5), Quchi (LI 11) of both sides, and lower limbs: Yinbai (SP 1) and Shenmai (BL 62) was conducted. Acupuncture on Jiache and Quchi was retained until the patient vellicated limbs. Punctured on Dicang (ST 4) if the patient had spasm on angulus oris, about 20 minutes every time, once a day. After the treatment, the patient had some improvements in the consciousness and electrophysiology. Significant pain reaction appeared, and the endings of extremities had some little movement. The patient could turn head and twist shoulder. Evoked potential in electromyogram (EMG) had certain amelioration.

To cure one patient with spinal cord injury (SCI), who hospitalized for both legs couldn't moving for 21 years after external injury, by using the olfactory ensheathing cells (OECs) transplant and acupuncture treatment. The patient was diagnosed as ischemic SCI (below the T11) according to the neurological test and MRI for dorsal vertebra. T11 spinous process was made to be the center mark through operation with the length of about 3 cm. The area between the atrophique spinal cord and normal spinal cord without vessel was selected and injected with OECs 0.5?109 L-1. Hemostasis, stitching, binging and fixation were performed respectively, and normal treatment was given by using antibiotics. At 14 days after operation, the temperature on skin increased a little, and acupuncture treatment and rehabilitation therapy were performed. The acupoints were as following: Baihui (DU21), Yintang (EX-HN3), Hegu (LI4), Zhongji (RN3), Guanyuan (RN4), Biguan (ST31), Fengshi (GB31), Futu (ST32), Dubi (EX-LE5), Zusanli (ST36), Xuehai (SP10), Yinlingquan (SP9), Sanyinjiao (SP6), Jiexi (ST41), Taixi (KI3), Fuliu (KI7), Taichong (LR3), and Shenmai (BL62). The treatment was given by mild reinforcing and attenuating with 30-minute retention. The rehabilitation: The needle pressing, manipulation for muscular atrophy, bicycle practicing, andimperative standing were performed. After 2 weeks, the skin temperature of the patient increased with obvious improvement in his right leg. The physical strength of the patient was reinforced with less perspiration, and the patient could walk 3 km without fatigue, which indicated that after OECs transplant, treatment with acupuncture, massage and other rehabilitation trainings could facilitated the improvement of SCI of the advance stage as well as the amelioration of muscular atrophy.

Objective To evaluate the imaging features of phyllodes tumor of the breast.Methods 35 patients with phyllodes tumor of the breast confirmed by surgery and histopathology were collected.All clinical and imaging findings were analyzed.Results 35 cases with 36 lesions were classified as benign (1 9 lesions),borderline (8 lesions),and malignant (9 lesions).The typical ap-pearances on mammography were ellipse (1 6/36)or lobulated (20/36)mass.Most of them were high density (30/36)and clear boundary (28/36).No evidence of calcification,spiculated margin,skin thickening,nipple retraction and enlargement of axillary lymph nodes were detected.On MRI,1 lesion presented as lobulated mass with nonuniform hypointensity on T1 WI,hyperintensity on T2 WI and DWI.The time-intensity curve was Ⅱ type on dynamic contrast enhancement MRI.Conclusion The clinical and ima-ging features of phyllodes tumor are characteristic.These features are helpful to preoperatively diagnose,the final diagnosis would confirmed by histopathology.

Objective To explore the effect of new hardener gathered cinnamyl alcohol (hardening of the oxygen ethyl alcohol)and anhydrous ethanol in the treatment of endometriosis cysts guided by ultrasound.Methods Two groups of cases,a group of 36 cases of endometriosis cyst in anhydrous ethanol sclerotherapy under the guidance of ultrasound(anhydrous ethanol group),another group of 30 cases of endometriosis cyst under the ultrasonic guided into 1% poly cinnamyl alcohol hardening treatment(poly cinnamyl alcohol group).Results Following -up every 3 month,the longest time was 2 years follow -up,the shortest time was 9 months.The anhydrous ethanol group:the to-tal effective rate was 95.0% (excluding a patients discontinue treatment due to severe pain),and the recurrence rate of 12.5% in the first year,second year recurrence rate 17.5%;The poly cinnamyl alcohol group:the total effective rate was 94.2%,the recurrence rate of in the first year was 11.7%,second year recurrence rate was 17.6%.Conclusion Anhydrous ethanol and cinnamyl alcohol hardening treatment of endometriosis cyst accuracy guided by ultrasound is high,the treatment effect,the recurrence rate has no obvious difference,but small poly cinnamyl alcohol irritating to human body,the patient has no obvious pain,easy to accept,and people feel ethanol treatment most patients with pain,with occasional patients discontinue treatment due to severe pain.

0.05). Conclusion We di d not find the difference between the two CYP2C19 phenotypes in relation to the acid-suppressing effect of esomeprazole.

Objective To investigate the causes of a suspected outbreak of lower respiratory tract infection (LR-TI)caused by carbapenem-resistant Acinetobacter baumannii (CRAB)in the general intensive care unit(ICU)of a hospital,and provide scientific evidence for effective control of healthcare-associated infection(HAI).Methods Epi-demiological investigation on patients infected with CRAB and on-site monitoring on ICU environmental hygiene from March 4 to 23,2014 were performed,active prevention and control measures were taken.Results A total of 7 patients developed CRAB infection,the total length of stay during epidemic period were 160 days,the infection density of LRTI was 43.75‰(7/160),4 of 5 patients at No.02 bed had CRAB LRTI.Univariate analysis revealed that adopting conventional sputum suction procedure was a risk factor;the qualified rate of bacterial count on the object surfaces was 31 .75% (20/63).CRAB was also isolated from outer frame of suction device of No.02 bed, which had the same antimicrobial pattern as CRAB from 7 patients’sputum.After taking a series of controlling measures,there was no CRAB infection case after March 24,2014,this outbreak was effectively controlled. Conclusion This suspected HAI outbreak may be caused by inadequate disinfection of outer frame of suction device contaminated by CRAB,and horizontally transmitted through manipulation of sputum suction by health care workers.Clean and disinfection of ICU environmental object surfaces is of great importance for preventing HAI.

Aim To investigate the effects of paeonol(Pae)in inhibiting the proliferation of HT-29 cell and probe into the possible molecule mechanism by quantitative and qualitative assay.Methods The inhibited rate and apoptotic rate of HT-29 cells were measured quantitatively by MTT assay.We were trying to find out the possible mechanism through the morphologic observation on paeonol-processed HT-29 cell line by TUNEL assay and immunocytochenical method.Results Pae,in the concentration of 0.024～1.504 ?mol?L~(-1),inhibited the proliferation of HT-29 cells in vitro,which showed obvious concentration-effect relationship;The inhibited rate of HT-29 cells was also increased when Pae(0.024～1.504 ?mol?L~(-1))was treated for 24,48,72 and 96 h,which showed obvious time-effect relationship.After treated with the concentration of 1.504 ?mol?L~(-1),the apoptotic rate of HT-29 cells significantly increased,which showed significant difference compared with control;We examined all the experimental groups by flow cytometry,which showed that sub-G_1 peak appeared before G_1 period and the cells in S period increased,while the cells in G_1、G_2 period decreased,the apoptotic rate of HT-29 cells gradually increased along with the increasing of paeonol concentration.The apoptotic rate in experimental groups vs control has significant difference(P

Objective To explore the mechanism of protective effect of Rhodioloside in cerebral ischemia-reperfusion rats and its relevance to phosphatidylinositol 3-kinases ( PI3-K)/protein serine-threonine kinases ( AKT) signaling pathway .Methods Forty eight Sprague-Dawley rats were randomly divided into four groups: sham-operation group , ischemia-reperfusion group , and Rhodiolo-side treatment groups (5 and 10 mg/kg).The model of right middle cerebral artery occlusion was established with thread ligation meth -od.The score of the neurological deficit was estimated 2 h followed by 24 h reperfusion.Histopathological changes were observed by hematoxylin-eosin(HE) staining.The infarct volume was measured with triphenyltetrazolium chloride (TTC) staining.Apoptotic cells were assessed with terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) method.The expressions of PI3-K and p-AKT were evaluated with immunohistochemistry .Results The score of the neurological deficit was decreased more ob-viously, the number of apoptotic were decreased more significantly , the expressions of PI3-K and p-AKT were increased more signifi-cantly in the Rhodioloside treatment groups (5 and 10 mg/kg) than in the ischemia-reperfusion group ( P <0.05).The score of the neurological deficit was decreased , the number of apoptotic was decreased , and the expressions of PI 3-K and p-AKT were increased in the Rhodioloside treatment group (10 mg/kg) than the Rhodioloside treatment group (5 mg/kg) ( P <0.05).Conclusions The protective mechanism of Rhodioloside therapy against cerebral ischemia r-eperfusion injury might be associated with activating the PI 3-K/AKT signaling pathway and then inhibiting neuronal apoptosis .

Objective To investigate the diagnostic value of the instant gastrointestinal ultrasound contrast agent in the digestive diseases. Methods Five hundred and seventy-nine patients received the examination of the color Doppler after they drinked the ultrasonic contrast agent. Then the results were analyzed by consistency analysis. Results There was high consistency between the two examinations in the normal control, gastritis, peptic ulcer, gastric cancer and gastric leiomyoma. The Kappa value was 0.768, 0.913, 0.925, 0.939 and 1.000, respectively. But the consistency in the gastric polyp was low , the Kappa value was 0.368. Conclusion The color Doppler through the instant gastrointestinal ultrasound contrast agent has high diagnostic value in the common diseases of the digestive system.