Objective To examine the changes of matrix metalloproteinase-9 (MMP-9) and tissue inhibitors of metalloproteinase-1 (TIMP-1) in gingival crevicular fluid after conventional mechanical therapy of adult patients with chronic periodontitis. Methods Gingival crevicular fluid samples from 15 patients and 15 healthy subjects were taken with filter paper strips by intra-pocket method initially, respectively, 1 month after scaling and root planning. MMP-9,TIMP-1 levels were measured by immunoblotting. Results MMP-9 levels in patients were redueed significantly after treatment[(2.2±1.4)AU vs (1.2±0.9)AU ]. The clinical parameters were positive correlation with MMP-9 level. TIMP-1 levels in patients were increased significantly after treatment[(1.3±1.2)AU vs (3.7±2.2)AU]. The clinical parameters were negative torrelation with TIMP-1 level. Conclusion MMP-9, TIMP-1 levels can act as objective parameters to evaluate the effect of periodontal therapy.

Objective To explore the use of urine LH test paper associating basal body temperature in ovulation monitoring and conception guiding in anovulatory infertility.Methods We used clomiphene to treat 100 anovulatory infertility patients.Then we recorded basal body temperature and used urine LH test paper in morning to determine whethere there is urine LH peak between the 12th to 20th day of menstrual cycle.If the patient become pregnant,we stop observation.If not,we observed them until the third treatment cycle completed.Result Before treatment,both the urine LH test paper and basal body temperature showed there was no ovulation.After treatmend,we observed 199 menstrual cycle.There were urine LH peak in 149 menstrual cycle.And there were double phase body temperature in 158 treat cycle.The lowest body temperature appeared in the urine LH peak day or after it.71.1%of the lowest body temperature emerged in one or two days after the urine LH peak appeared.76 patients in our study become pregnant,pregnancy rate is 76%.Conclusion Urine LH test paper associating basal body temperature is convenient and easy tO learn.It is a good way in ovulation monitoring and conception guiding.

Objectives To determine the predictive effect of the transvaginal ultrasonographic cervical measurement in induction of labor among the nulliparous. Methods This prospective observational study recruited women with singleton gestation scheduled for induction of labor at ≥37 weeks. All the pregnancy women were nulliparous. Transvaginal ultrasonographic cervical measurement was performed and Bishop score was detemined, each by operators masked to the other measurement. Data were collected on parity, gestational age, mode of delivery, induction agent, induction onset of labor interval, Bishop score and cervical measurement. Results A total 121 women were included. A stepwise regression model identified cervical length was the best linear predictor of induction to onset of labor interval. ( r=0.67, P

Objective:To establish nursing quality indicator system for freestanding hemodialysis facilities and identify the weight values of indicators.Methods:With the structure-process-outcome quality model as the framework, the literature analysis method, field survey and semi-structure interview were used to form the indicators item pool. Through Delphi method, experts were consulted for two rounds. The indicator weight was calculated by AHP.Results:The indicator system of this study included 3 primary indicators, 15 secondary indicators and 46 tertiary indicators. Among the primary indicators, the process quality indicator had the largest weight (0.493), followed by the outcome quality indicator (0.310), and the structure quality indicator had the smallest weight (0.196).The process quality were the main evaluation contents. According to the indicator weight, the main manpower allocation (0.131) in secondary indicators had the highest weight, and the proportion of nurses with different hemodialysis work experiences (0.108) in the tertiary indicators had the highest weight.Conclusion:The indicator system of this study is scientific and integrated, which can be used as a nursing quality evaluation tool to verify its practicability in clinical nursing practice.

Objective To investigate the effects of C-terminal fragment of parathyroid hormonerelated protein (PTHrP107-139) on bone mineral density (BMD), bone histomorphometry and biomechanical properties in ovariectomized (OVX) rats and its effect on bone metabolism is also explored. Methods Forty 4-month old female Wistar rats in which 30 were ovariectomized and then divided into 3 groups: the placebo, the PTHrPC and the CT groups, the other 10 rats were Sham-operated as the control group (Sham). Five weeks later, the rats of PTHrPC and CT groups were subcutaneously injected with PTHrP107-139 (40 μg/kg) and Salmon Calcitonin (15 U/kg) respectively once every other day. The rats of the placebo and sham groups were injected with 0.2 ml saline once every other day. After treatment of 12 weeks, all rats were sacrificed and all samples were collected and analyzed. Results ① Compared with the placebo, the BMD and bone strength of PTHrPC and CT groups were significantly increased (P<0.05). ② Histomorphometry revealed that the tetracycline labeled bone surfaces, osteoid surfaces, mineral apposition rate and bone resorption rate were remarkably decreased in PTHrPC, and CT groups comparing with those of the placebo group. Conclusion Cter-minal PTHrP107-139 is effective in increasing the BMD, bone strength and quality when administered intermittently to ovariectomy-induced osteoporotic rats. Its increasing in bone quality may relate to reducing bone turnover and inhibiting resorption.

Drug Compounding ; Ivermectin ; analogs & derivatives ; toxicity ; Neurotoxicity Syndromes ; etiology ; therapy ; Pyridazines ; toxicity

Objective:To investigate pathological changes and the expression of the matrix metalloproteinases in rat EAE.Methods:The pathological changes of EAE were studied in Wistar rat with the aid of light and electron microscope and the expression and distribution of MMP-2,MMP-9 in different tissues were detected with the method of immunohistochemistry.Results:Light microscopy showed inflammatory cuff around small blood vessels were evident, disintegration and disappear of myelin sheath were observed. Electron microscopy showed a lot of loose and fragmental spiremes of myelin sheath, the component of axons and nissl bodies in neurons were disappeared. MMP-2,-9 were expressed intensively in vascular endothelial cells, meninges and accumulative inflammatory cells.Conclusion:MMPs take the roles in every aspect of the pathological changes in EAE, it can destroy the blood brain-barrier, degrade the myeline sheath, damage the axons and generate immunogens.

OBJECTIVE To investigate the distribution and resistance of non-fermenting bacteria isolated from our hospital and offer the basis for the treatment of bacterial infection.METHODS Totally 2177 strains of non-fermenting bacteria were isolated from clinical sputum samples between Jan 2006 and Dec 2008.All of the isolated bacteria were identified with API identified test(API Inc,France) and Kirby-Bauer(K-B) test used for the antibiotics susceptive test.RESULTS From them the most common bacteria were Pseudomonas auruginosa(34.9%),followed by Acinetobacter baumannii(28.4%) and Stenotrophomonas maltophilia(11.4%).These bacteria had various resistances to the tested antibiotics.CONCLUSIONS Non-fermenting bacteria have high isolate rate and multi-antibiotic resistance,so antibiotics should be correctly used under the guidance of antibiotic susceptibility test.

Objective To investigate the therapeutic effects of human parathyroid hormone related protein (PTHrP1-34) on osteoporosis of ovariectomized osteoporotic rats. Methods Sixty 4-month-old female Wislar rats were involved in this study and 40 of them were ovariectomized and another 20 received sham operation. After 6 weeks of ovariectomy the osteoporosis model was confirmed by examing 10 ovariectomized and sham-operated rats. The 30 osteoporotic rats were randomly divided into 3 treatment groups, i.e. PTHrP, estradiol and placebo. Human 40 ?g/kg PTHrP1-34 was subcutaneously injected once daily to PTHrP group and the estradiol group was injected with 40 ?g/kg estradiol benzoate once every 3 days.The placebo and shamoperated rats were given 0.2 ml saline every 3 days. The bone mineral density (BMD), bone histomorphology, the bone weight of dry and ash and serum Ca,P,alkaline phosphatase (ALP) were measured after 3 months' therapy. Results After 6 weeks of ovariectomy, the lumbar BMD of ovariectomized rats were significantly declined compared with those of the sham-operated rats. After 12 weeks treatment the femoral and lumbar BMD and the rate of bone weight of dry and ash in the PTHrP group were increased obviously compared with those of placebo groups.There was no significant difference between PTHrP group and estradiol group, in PTHrP group the percent age of trabecular area,trabecular width,osteoblast surface and mineral apposition rate were obviously higher than those in placebo group.Conclusion Treatment with 40 ?g/kg dose of hPTHrP1-34 administered once daily is effective in treating ovariectomy-induced osteoporosis.

Objective:To investigate establishing the multiple clinical processes model and pathological characteristics of EAE model,and to provide the experimental value for reseaching MS. Methods:The animal model was established in Wistar rat by immunization with complete Freund adjuvant and GPSCH, the pathological changes of EAE were studied with the aid of light microscopy. Results : We can divide the EAE into five types by pathological changes and clinical manifestation: acute EAE, relapsing-remitting EAE, persistent progress EAE,benigh form EAE and delitescence EAE. Every type mainly showed congestion and inflammatory cuff of small blood vessels,disintegration and disappear of myelin sheath and degeneration of neurons,especially in spinal cord. Conclusion:Multiple clinical processes on EAE model of Wistar rat were established for the first time.it is an ideal animal model in studying MS.