0.05). Conclusion Tangkening may be another therapy choice of Diabetes.

Objective To investigate the effects of safflor yellow (SY) injection on acute lung injury (ALI) rats induced by oleic acid (OA) and its potential mechanism. Methods Wistar rats were randomly divided into six groups,including control group,OA group,OA+10 mg/kg anisodamine group,OA+8 mg/kg SY group,OA+16 mg/kg SY group,and OA+32 mg/kg SY group. Normal saline or anisodamine or SY were pretreated before 0.18 g/kg OA iv injection. The arterial partial pressure of oxygen,the pulmonary water content index,and MPO activity in lung tissue were determined; The mRNA level of TNF-?,IL-1?,IL-6,ICAM-1,and VCAM-1 were measured by RT-PCR; And NF-?B p65 protein in nucleus observed by immunohistochemical technology,while the level of p38 MAPK protein phosphorylation in lung tissue was analyzed by Western blotting. Results The arterial partial pressure of oxygen in all SY groups was higher than that in OA group,while the pulmonary water content index and MPO activity were lower than those in OA group,as well as the mRNA level of the above inflammatory cytokines and the NF-?B p65 in nucleus and level of p38 MAPK phosphorylation. Conclusion SY could alleviate the lung tissue edema,increase the arterial partial pressure of oxygen,and depress MPO activity in ALI rat induced by OA. The SY mechanism of attenuating the acute lung injury may be associated with inhibiting the p38 phosphorylation and NF-?B activation and reducing inflammatory factors expression.

Objective To observe the latency and amplitude characteristics of event-related potential P300 under different target stimu-lus. Methods During January to December, 2014, 35 patients with memory impairment received P300 test under the percentage of target stimuli of 0.10 and 0.25. The latency, amplitude and area under amplitude were compared. Results There was no significant difference in la-tency (t<0.256, P>0.800), but the amplitude and area under amplitude increased under 0.10 compared with those under 0.25 (t>4.259, P<0.05). Conclusion Less target stimulation may improve the patients' attention and increase amplitude of P300, but no more interference in la-tency, suggesting latency of P300 is more stable for assessment of cognitive function.

Vitamin C injections are a national essential drug with wide clinical applications and instable property. In order to pro-vide the reference for further analysis on the quality status of vitamin C injections and improve the quality,the research on the stability, quality control and analysis methods was summarized in the study.

Objective To investigate the correlations between obsessive-compulsive symptoms and autism-spectrum quotient and explore if systemizing is the mediator between them. Methods With Padua Inventory (PZ) ,AQ and Systemizing Quotient (SQ), 1901 college students were assessed with stratified random sampling survey. Results Compared with the femal students, score of systemizing in male students had a significant increase ( (26. 773 ± 9.225 )vs (22.017 ± 7 ± 934), P ＜0.01 ). AQ total score was significantly positive correlated with PI total score( r=0. 336, P＜0.01 ),and correlation was also found among SQ,AQ and PI in different degrees. the mediating effect of systemizing was significant. The regression equation was AQ = 18. 942 +0. 064PI -0. 022SQ (P ＜ 0.01 ). Conclusion There are positive correlation between obsessive-compulsive symptoms and AQ in college students, and systemizing is the mediator between above symptoms.

0.05).Conclusion Tripterygium wilfordii,combined with routine treatment,appeared to decrease 24-hour proteinuria in a certain extent and did not adversely affect liver function,renal function and the blood routine test in most patients with diabetic nephropathy.

AIM:To investigate the effects of brain-derived neurotrophic factor(BDNF)on extracellular proteolytic enzymes including matrix metalloproteinases(MMPs)and serine proteases,in particular,the urokinase-type plasminogen activator(uPA)-plasmin system in a human umbilical vein endothelial cell(HUVEC)model.METHODS:The HUVEC was activated with different doses of BDNF(25-200 ?g/L)for different time period(6-48 h).Reverse transcriptase-polymerase chain reaction(RT-PCR)was used to assay MMP-2,MMP-9,TIMP-1,and TIMP-2 mRNA in HUVEC.The cultured conditioned medium was analyzed for MMP and uPA activity by gelatin zymography and fibrin zymography,respectively.uPA,PAI-1,TIMP-1,and TIMP-2 were quantified by Western blotting analysis.RESULTS:The stimulation of serum-starved HUVECs with BDNF caused marked increase in MMP-2 and MMP-9 mRNA expression and induced the pro-MMP-2 and pro-MMP-9 activation without significant differences in proliferation.However,BDNF had no effect on TIMP-1 and TIMP-2 production.BDNF increased uPA and PAI-1 production in a dose dependent manner up to 100 ?g/L,while effects of 200 ?g/L were approximately equal to those of 100 ?g/L.BDNF stimulated uPA and PAI-1 production beyond that in control cultures from 12 h until 48 h after BDNF addition.Protease activity for uPA was also increased by BDNF in a dose dependent manner.CONCLUSION:BDNF activates MMP and uPA/PAI-1 proteolytic network in HUVEC.

Differential diagnosis of benign and malignant ascites is of a great value in determining etiology, evaluating prognosis, and determining therapy regimens. There are many diagnostic methods for ascites, but up to now, no method can achieve high sensitivity and specificity and meet the requirements of clinical economics and feasibility at the same time. Therefore, searching for an ideal method for differential diagnosis of ascites has become an important topic of current research. This article describes the clinical value of diagnostic methods, such as molecular biological detection, cytological examination, combined determination of tumor markers, and routine tests, in the differential diagnosis of benign and malignant ascites, analyzes the diagnostic efficiency of related biomarkers, and points out that combined determination of various parameters has an increased value in the differential diagnosis of benign and malignant ascites.