Crohn's disease and intestinal tuberculosis are intestinal chronic granuloma.The mis-diagnosing rate is high.It is a great challenge to distinguish Crohn's disease from intestinal tuberculosis.The prognosis is quite different between Crohn's disease and intestinal tuberculosis.So,it's very important to correctly identify Crohn's disease and intestinal tuberculosis.

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[Objective]To study the difference of efficacy of Shuxuetong injection from other medicine in the treatment of acute cerebral infarction.[Methods]27 comparative studies of Shuxuetong and other medicine in the treatment of acute cerebral infarction were analyzed by meta-ananlysis about antidepressants indicators.[Results]The clinical efficacy of Shuxuetong was obviously superior to that in control group.The hemorheology indicators(the whole blood viscosity in high shear rates,whole blood viscosity,fibrinogen change)of Shuxuetong were obviously superior to that in control group,but the whole blood viscosity in low Shuxuetong had no significant difference from control group.[Conclusion]Shuxuetong has positive effect on the treatment of acute cerebral infarction compared with other medicine.

AIM: To determine the inhibition of VEGF small interfering RNA (siRNA) on expression of VEGF protein and SMMC 7721 cell growth. METHODS: Nine VEGFsiRNA sequences with nineteen nucleotides were designed under the assistance of computer. The inhibitory effect of VEGFsiRNA was analyzed by CCK8. The protein level of VEGF in the media was determined by ELISA. The change of cell cycle was detected by flow cytometry. RESULTS: All the VEGFsiRNA were capable of inhibiting the proliferation of SMMC 7721 cells significantly compared with control and lipofectamine control, while the inhibitory effects of VEGFsiRNA2, VEGFsiRNA4, VEGFsiRNA6, VEGFsiRNA7, VEGFsiRNA8 and VEGFsiRNA9 were better than that of antisense oligodeoxynucleotide. All the VEGFsiRNA reduced the expression of VEGF protein. The effect of VEGFsiRNA7 and VEGFsiRNA2 were the best with the inhibitory rates of 52.65% and 50.43%, respectively. VEGFsiRNAs induced the S arrest in SMMC 7721 cells. The signs of apoptosis in SMMC 7721 cells induced by VEGFsiRNA were not observed. CONCLUSION: VEGFsiRNA sequences were designed and synthesized successfully. VEGFsiRNA effectively inhibited the proliferation of SMMC 7721 cells and reduced the level of VEGF protein.

Transcription factors T-bet/GATA3 are one of the most fascinating areas of immunology today.Rencently,information has been accumulated to clarity T-bet/GATA3 roles in etiology and progression of certain diseases.This review focuses on the relationship between T-bet/GATA3 and diseases.

Objective Nine VEGF siRNAs were designed and their efficiencys were evaluated for introducing new and effective design rules of siRNA.Methods Nine VEGF siRNA sequences with nineteen nucleic acids were designed.After construction in vitro,VEGF siRNAs were transferred into neuroglioma cells by lipofectamine.The level of VEGF protein in the media was determined by ELISA 48 hours after transferring.The relationship between the characteristics of VEGF siRNA and its' interfering effect were analyzed.Results A quantitative parameter called H-b index were used to characterize the secondary structure of VEGF mRNA.A low value of the H-b index would mean that the structure of mRNA is loose,in which the nucleotides of the targeting region are likely to be accessible by siRNA and the effects are better.The experiments showed a negative correlation between the suppression of VEGF protein expression and H-b index(r=-0.498).The H-b indexs of siRNAs targeting VEGF mRNA 386～406 and 401～421 position were less,however the effects of suppression were better.The siRNAs conferred a strong RNAi activity with A residue at position 6 and A/U residues at position 19 of sense strand of siRNAs.Thethermodynamic properties of siRNA play a critical role in the transferring effects.The lower average of internal stability values for positions 9～14 of the antisense strand is facilitating the interfering effect.The lower binding energy is the more stable incorporation between antisense strand of siRNA and mRNA present,the more favorable is for RISC cleaving mRNA.Conclusions The sequences of VEGF siRNA have been designed successfully and effectively inhibited the expression of VEGF protein.

To discuss the ultrasonic testing intelligent infusion pump tubing bubble method. When the ultrasonic wave goes through the interface of two different media, the reflection occurs, refraction, transmission and other physical phenomena can occur because of a large extent acoustic impedance difference between water and air, which can be used in this physical phenomenon, by monitoring the ultrasonic energy (output voltage) can be obtained if the extent of the presence of bubbles and bubble exists within the infusion tube. By measuring the penetration of ultrasonic energy, we detected air bubbles within the infusion line. Ultrasound can accurately detect air bubbles in the infusion line, which is the main method of detecting air bubbles for infusion pump.

Interleukin-33 is regarded as the orphan receptor ST2 that belongs to the cytokine family of interleukin-1 in 2005. IL-33 is found to play a potentially pathogenic roles in the autoimmune disease, allergic disease, vascular disease, inflammatory disease and it might be a useful sera marker for the diagnosis and prognosis in related diseases. Here we make a review on the relationship between IL-33 and autoimmune disease in recent years.

On the view of modern risk management,4 kinds of risks are found out through joint-venture medical equipment actuality investigation.Against these risks,the risk management system of hospital medical equipment is set up to identify and diminish the risks in joint-venture of medical equipment,included management flow optimization,risk management mechanism, risk avoidance and risk analysis.

Objective:To evaluate the performance for the Sysmex XN20 A1 automation blood cell analyzer.Methods: The precision, contaminative rate, linear range, blank, accuracy and various sample models were verified, and low values of blood platelet were compared with the methods of microscopy and dye.Results:The contaminative rate was lower than 0.4%. The linear arrange and precision were well for the analyzer. The values of RBC, HGB and PLT were within the range of 1±0.05 and the correlation coefficients were higher than 0.975. The bias of average and constant values by the accuracy verification samples test met the demands of accuracy. And the relative differences of various sample models meet the requirements of the comparability. The variable coefficient of low values of PLT was lower than 4% by the dye method.Conclusion: The Sysmex XN20 A1 automation blood cell analyzer has the characteristics of perfect precision, accuracy, low contaminative rate, broad linear arrange and good repeatability for the low level PLT. It can be applied in clinical laboratory.