Objective To investigate the effects of different cardiopulmonary resuscitation (CPR) qualifies under Q-CPR control on hemodynamics and aerobic metabolism in pigs with cardiac arrest. Method After eighteen pigs (mass [ (30+ 1) kg] were anesthetized,a Swan-Ganz catheter was positioned through the right femoral vein and linked with a continuous cardiac output monitor. An electrode cable was positioned into right ventricle through left internal jugular vein. Then different catheters were positioned in the arteriae aorta, carotid artery and fight atrium successively. Ventricular fibrillation (VF) was induced by a programmed electrical stimulation equipment. All animals underwent 4 minutes of untreated VF and then were randomly assigned to either standard CPR or nonstandard CPR. A Philips HeartStart MRx Monitor/Defibrillator was used to ensure qualifies of chest compression (e.g., depth, rate and chest recovery) by Q-CPR technology. Pigs in standard CPR group were treated with standard compression with a compression rate of 100 beat/min, a compression-ventilation ratio of 30:2, and a depth of with 38～51 mm with 100% decompression. Pigs in nonstmdard CPR group were treated with nonstandard compression with a depth of 60%～70% of standard depth with unsatisfactory decompression. Defibrillations were administered after 9 minutes of compressions and vetilations. Cardiac output (CO), mean aortic pressure (MAP) and coronary perfusion pressure (CPP) were recorded at different periods. Oxygen delivery (DO2) and oxygen consumption (VO2) were calculated according to blood gas analysis. At last,number of the survivals was recorded.Data were calculated by SPSS 11.5 statistics software. Statistical analysis was performed using Chi-Square Test and two independent samples t testing. Results The return of spontaneous circulation (ROSC) achievement ratio with nonstandard CPR was 28.6% and with standard CPR was significantly greater (90.9%, P=0.013).Hemodynamics (e. g., CPP, CO and MAP) and systemic oxygenation with standard CPR were much better. Conclusions In this animal model of cardiac arresr in pigs, standard CPR could improve hemodynarmics by increasing CPP and CO. And standard CPR could beneficial aerobic metabolism by increasing DO2 and VO2. As a result, ROSC achievement ratio of pigs with standard CPR was much greater.

Objective To study the therapeutic effect of pulse-activating injection on acute poisoning by pa-raquat(PQ). Methods 50 Sprague-Dawley (SD) rats were randomly divided into five experimental groups: blank group, negative control group, positive control group, low-dose pulse-activating injection group (LDG), and high-dose pulse-activating injection group(HDG) (n = 10 for each group). Blank group were injected with normal suline,30 ml/kg,and other groups were established as acute paraquat poisoning models. Macroscopic and histopathological ex-aminations were performed and biological indexes were measured for the lung specimens. The indexes included lung wet weight/dry weight,the rats of neutrophils and protein content in the pulmonary alveolar lavage fluid. In the mean time, the level of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) both in plasma and bronchoalveolar lavage flui(BALF) were detected. Results Compared with that in negative control group,lung congestion and lung edema of LDG group were mitigated; and the MDA level decreased from (5.04± 0.50) nmol/ml,(1. 19±0.18) nmol/ml to (4.04±0.21) nmol/ml,(0.79±0.04) nmol/ml both in plasma and BALF;the SOD activities were increased from (123.30±20. 39) U/ml, (26.43±2.22) U/ml to (277.09± 11.66) U/ml,(37.10± 2.49) U/ml as well; the GSH-Px activities were increased from (1796.63 ±81. 12) U/ml, (598.24 ± 62.50) U/ml to (2151.54 ± 148.32) U/ml, ( 1788.44 ± 175.11 ) U/ml as well ( P < 0. 01 ). Conclusions Administration of pulse-activating injection could improve the lipid peroxidation damage caused by a-cute poisoning of PQ.

Objective To investigate the protective effect of non-wounded ischemic preconditioning (N-WIP) on lung ischemia/reperfusion (I/R) injury.Methods Two animal models with N-WIP and the classical ischemic preconditioning respectively were used. Forty New Zealand White rabbits were randomly divided into 4 groups, each group having 10 animals: control group (C), ischemia/reperfusion group (I/R), classical ischemic preconditioning group (C-IP), and N-WIP group. Lung injury was assessed by arterial oxygen tension (PaO2), wet to dry weight ratio (W/D), activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) and level of malondialdehyde (MDA).Results In I/R group, PaO2 was descended constantly, especially within 30 min after reperfusion. PaO2 and the activities of SOD, GSH-PX in serum and lung tissue in N-WIP group and C-IP group were both significantly higher than those in I/R group (P

Objective To explore the effect of sodium fluoride(NaF) on the tumor markers of human embryonic bronchial epithelium (HEBE) cells.Methods HEBE cells were collected from the human abortive fetues.The cells were exposed to NaF of several concentrations for 36 h.After rinsed,the cells were incubated for 36 h again.The NaF toxicity to HEBE cells was detected using MTT method.The supernatant was collected and the lung tumor markers were detected using ELISA method.Results NaF was toxic to the HEBE cells,and the toxicity was increased with the NaF doses.There were few survival HEBE cells at 6 mmol/L NaF.The tumor markers in both of the control and experiment groups were very low,and no significant difference had been seen between them.Conclusion NaF may damage HEBE cells,but may not influence the tumor markers of HEBE cells.

Objective To investigate effect on telomerase activity of Hep-2 cells treated by anticancer drugs(hydroxycamptothecine, cisplatin and cytoxan).Methods By MTT method,we measured the 50% inhibitory concentration(IC 50 ) at 72h,and compared to untreated control cells. Telomerase activity of Hep-2 cells treated by the drugs in different concentration based on IC 50 for different time was observed by Telomeric Repeat Amplification Protocol with ELISA(TRAP-ELISA).Results Hydroxycamptothecine and cytoxan could inhibit proliferation of Hep-2 and down-regulate telomerase activity of Hep-2 cell. However, cisplatin promoted proliferation of Hep-2 and up-regulated telomerase activity of Hep-2 cell.Conclusions Hydroxycamptothecine and cytoxan could down-regulate telomerase activity of Hep-2 cell by direct or indirect pattern, which may correlate with drug concentration and time-dependent pattern.Cisplatin could up-regulate telomerase activity of Hep-2 cell, which mechanism is not clear.

[Objective]To explore the result of total hip arthroplasty(THA) performed in patients with osteoarthritis secondary to developmental dysplasia of the hip(DDH) and the degree of hip dysplasia on the treatment result. [Method]Forty-seven cases(55 hips) with osteoarthritis secondary to DDH were treated with THA.According to the classification of Crowe,23 hips were in classⅠ,19 in classⅡand 13 in class Ⅲ/Ⅳ.Before operation,the morphological changes of acetabula and femurs were evaluated with helical CT.During the operation,the acetabula were reconstructed at the level of real acetabula,the femoral canal was carefully enlarged and the small and straight femoral protheses were employed.The Harris hip score was conducted in each patients preoperatively and postoperatively.[Result]The Harris hip score in class Ⅰ,Ⅱand Ⅲ/Ⅳ were from 50.7?7.6,44.9?6.2 and 41.1?8.2 preoperative to 90.6?3.7,87.3?4.5 and 82.7?7.3 postoperative,respectively.There was significant difference between them(P

Objective To explore the relationship between blood pressure and hemorrhagic cerebral infarction. Methods Retrospectively reviewed clinical data of 242 patients with acute cerebral infarction, then all patients with hemorrhagic cerebral infarction were selected. The distributions of different level of blood pressure (＞180/110mm Hg;90/70～180/110mm Hg;＜90/70mm Hg) were compared between the patients with or without hemorrhagic cerebral infarction, as the same, different prognosis were compared among the patients with hemorrhagic cerebral infarction based on the different level of blood pressure. Results Of the total patients with acute cerebral infarction,4. 1%(10) presented with blood pressure＜90/70mm Hg,88. 0%(213) with blood pressure 90/70～180/110mm Hg and 7.9%(19) with blood pressure＞180/110mm Hg. In all the patients,20 cases(8. 3%) with hemorrhagic cerebral infarction,among which,60.0%(12) patients with blood pressure＞180/110mm Hg and with 7 cases died, 10.0%(2) patients with blood pressure＜90/70mm Hg and with 1 case died,30.0%(6) patients with blood pressure 90/70～180/110mm Hg and with 2 cases died. The distribution of patients with blood pressure＞180/110mm Hg in those two type of cerebral infraction were statistically significant (P=0. 000) ,the ratio seemed higher in patients with hemorrhagic cerebral infarction, and among the patients with hemorrhagic cerebral infarction, the distribution of prognosis were the same statistically significant based on the diffetent level of blood pressure, among which, patients with blood pressure＞180/110mm Hg(P =0.041) or＜90/70mm Hg(P =0.037) seemed like to have a higher mortality. Conclusion Severe high blood pressure(＞180/110mm Hg) should be one of the high risk factors for patients with hemorrhagic cerebral infarction,when blood pressure＞180/110 mm Hg or ＜90/70mm Hg happened in the pathogenesis, patients always be with poor outcome. So, making proper control strategy of blood pressure for patients with hemorrhagic cerebral infarction was with significance to defend its' happening and prognosis' improvement.

Objective To investigate the targeted killing effect of mucin 1 single chain antibody targeting, lentivirus mediated suicide gene therapy and ganciclovir(GCV) in mucin 1 + ovarian epithelial carcinoma cells Methods Mucin 1 single chain antibody targeting lentivirus produced by packaging cell line 293T transduced herpes simplex virus thymidine kinase (HSV tk) gene into the ovarian epithelial cancer cell line SKOV3 (MUC1 +) The infection effect was observed through fluorescence microscopy Polymerase chain reaction (PCR) and reverse transcription PCR (RT PCR) were resorted to demonstrate the successful transduction and transcription of the HSV tk gene After administration of GCV, changes of those cells were observed through optical microscopy The cytotoxicity efficacy of HSV tk/GCV system was evaluated by methyl thiazolyl tetrazolium method Results It was observed through fluorescence microscopy that anti MUC1 directed lentivirus can specificly infect MUC1 + ovarian cancer cells A fragment of 600 bp was generated through PCR and RT PCR which indicated successful transduction and transcription of the HSV tk gene in SKOV3 cells Changes of cells followed by administration of GCV were observed with optical microscopy Significant cytotoxicity efficacy of GCV to SKOV3 was observed Conclusions The HSV tk gene can be targetedly transducted into MUC1 + ovarian cancer cell line under the mediation of anti MUC1 directed lentivirus, and such HSV tk/GCV system has targetingly killing effect on MUC1 + cancer cells

Objective:To observe the effect of Xinmailong injection (XI) on free calcium ( Ca2+) content in rat myocardial cells. Methods:Free Ca2+content in rats treated with various dosages of XI were measured by fluorospectrophotometry. Results:Xinmailong injection significantly increased free Ca2+content and the content was 169.18?11.64, 233.26?10.69 and 164.25?10.34 nmol?L-1 in 0.19 g?L-1 , 0.38 g?L-1 and 0.76 g?L-1 XI group respectively, the differences being significant as compared with 120.64?3.02 nmol?L-1 in the control group (P

Objective To investigate specific anti-leukemia immune response induced by tumor lysates pulsed dendritic cells(TP-DC)in syngeneic bone marrow-transplanted mice.Methods From June 2002 to June 2003,the bone marrow cells were induced by using granulocyte-macrophage colony stimulating factor(mGM-CSF)and interleukin-4(mIL-4).DC was pulsed with L7212 tumor lysates at the immature stage.TP-DC was harvested on 7th day.615 mice immunized thrice with L7212 TP-DC starting at day 7 following BMT.Additional control groups were set up.The survival was observed.Results Immunization using TP-DC could induce L7212 cells-specific CTL responses that were statistically significant compared with control groups(F=391.77,P