Objective To study the route of intrauterine infection of chlamydia trachomatis (CT) Methods Seven hundred and seventy two cervical samples from in women and 105 matched maternal labom neonatal samples composed of cervical samples,cord blood, amniotic fluid, conjunctival and nasopharyngeal samples of neonate were detected by PCR SSCP and DNA sequencing technique Results CT were detected in 87 of 772 (11 3%) cervical samples In the 81 matched maternal infant samples from pregnant women with cervical CT positive, CT were not detected in all of the cord blood samples In the 30 CT positive neonatal samples, 26 were from cases of vaginal delivery and 4 from cases of caesarean section Statistical analysis showed a significant difference between the groups of caesarean section and the vaginal delivery ( P

Objective To study the effects of integration of medical treatment and care giving on patients' rehabilitation efficacy after hip replacement. Methods One hundred and six patients having undergone hip replacement in rehabilitation ward were randomly divided into the control group and the observation group from August 2013 to February 2015, 53 cases in each group. The control group received the traditional mode while taking the new integration of medical treatment in the observation group. The two groups were compared in terms of score on patients'ability of daily living, Harris score and patients'satisfaction. Result The score on patients'ability in daily living, the Harris hip score, joint function score were all higher than those of the control group (all P < 0.05). Conclusion The new integration of medical treatment can effectively improve the patients'ability in daily living , the hip joint function and the satisfaction of patients, reduce the potential complications after surgery and optimize the process of management in rehabilitation ward, which is worthy of promoting.

We reported a clinical caseof gas gangrene in the lower back and buttocks due to local intramuscular injection. A 43-year-old male was treated by local private clinic for low back pain. He underwent local intramuscular injection in his lower back and buttocks. 5 or 6 hours after the treatment,he felt worsen of low back pain (VAS 5 points)with a fever. After one day of treatment, the lower back pain was even worse(VAS 8 points). And he had soy-sauce urine and a high fever. The results of laboratory examination and clinical manifestation suggested multiple organ failure within a short period of time. Imaging (CT) suggested large area of gas and muscle necrosis in the lower back and buttocks. Considering the progress of the disease, emergency surgery on lumbar back and right buttocks was performed after hospitalization. Rapid bacterial smear reported gram-positive coarse bacteria. Anaerobic culture suggested clostridium perfringens. Aerobic culture result did not show any bacterial growth. According to the clinical manifestation, imaging (CT), intraoperative judgment and postoperative anaerobic culture results, the clinical diagnosis was gas gangrene. Gas gangrene is a special infectious disease in clinic, which can be characterized by low morbidity, difficult early diagnosis,rapid disease progression and high mortality. Sinceintramuscular injection leaded to this disease in our case, the importance of standardized aseptic procedures should be emphasized.

ObjectiveTo discuss the application of clinical pathway in the health education for spinal cord injured patients in rehabilitation department. Methods56 patients with spinal cord injury were divided into 2 groups: treatment group and control group. Both groups were cared with holistic nursing. However, the health education to the treatment group was given by clinical pathway, while to the control group by traditional way. On the day of discharge, the health education standard and the satisfaction of patients were investigated. ResultsBoth the rates of standard in health education and satisfaction were more in treatment group than in control group. ConclusionApplication of clinical pathway can regulate the health educational behavior, improve the communication between nurses and patients, and the quality of nursing.

Objective To design a new method of determining optical properties of biological tissue by measuring light intensity distribution.Methods The light distribution in Intralipid-10％ suspension at 650 nm was measured by optical fiber probe.The optical parameters were derived from nonlinear regression of the diffusion equation for intensity.The distribution and anisotropy of intensity was abtained by the determined optical parameters and the diffusion equation for intensity.ResultsThis new method was feasible by comparing the experimental data with published results.ConclusionThe experiment results would provide useful information for radiative transfer equation,marine optical remote sensing measurement and laser therapeutic applications.-

BACKGROUND: Poor mental health status of nursing students can affect their learning and living. OBJECTIVE: To explore mental health status of nursing students at different studying stages.DESIGN: Grouping stratified sampling survey on the basis of nursing students studying in higherschool.SETTING: Nursing Department of the Fourth Military Medical University of Chinese PLA, Laboratory Center of Basic Faulty, and Department of Rehabilitative Physioltherapy of Xijing Hospital.PARTICIPANTS: Totally 515 nursing students at different studying stages of three educational levels (the technical secondary school level, junior college level and undergraduate level) were observed with grouping stratified sampling in December 2004.METHODS: Symptom checklist-90(SCL-90) was used for survey. SCL-90 contained 90 items, being interpreted in the context of nine symptom dimensions, including somatization, obsessive-compulsive, interpersonal sensitivity, depression, anxiety, hostility, phobic anxiety, paranoid ideation, and psychoticism. Each item was scored from grade Ⅰ to grade Ⅴ (symptomless, mild, moderate, little severe, and severe). Scores of total items and 9 symptoms were recorded. The more the scores of total items were, the lower the total level of mental health was; the more the scores of any symptom was, the severer the symptom of mental health was. Data were analyzed with t test. MAIN OUTCOME MEASURES: Scores of total items and 9 symptoms. RESULTS: Totally 515 nursing students were randomly selected in this survey except 5 students because of uncompleted survey, and the response rate was 99.0%. There were 501 qualified questionnaires, which were accounted for 98.2%. Mental health status of nursing students at various stages was different: the score of nursing students in grade 1 and 3 was higher than that in grade 2 in technical secondary school; the score of nursing students was the highest in grade 2 in junior college; and the score of nursing students was the highest in grade 3 at undergraduate level. The order of SCL-90 scores was obsessive-compulsive, interpersonal sensitivity and depression from high to low.CONCLUSION: SCL-90 scores of nursing students are high in grade 1and grade 3 at the technical secondary school level and also in middle grade at junior college level and undergraduate level. The main mental psychological symptoms are obsessive-compulsive, interpersonal sensitivity and depression. Psychological predisposition of nursing students should be cultured according to different psychological characteristics at various learning stages.

Objective To use the enzyme linked immunosorbent (ELISA)to detect the hepatitis B virus (HBV)markers,and to perform the HBsAg quantitation and the HBV load detection for understanding the HBV carrying and viral replication situation when single HBcAb positive or both HBcAb and HBeAb positive.Methods The HBV markers HBsAg,HBsAb,HBeAg,HBeAb and HBcAb were detected with ELISA.1 098 cases of HBcAb positive,966 cases of both HBeAb and HBcAb positive and 832 cases of all HBV markers negative as control were selected and quantitatively re-detected HBsAg by using the chemiluminescence meth-od.The HBV load was detected by using the PCR method.Results Among 1 098 cases of single HBcAb positive,436 cases (39.7%)of HBsAg quantitation and 230 cases (20.9%)of PCR-DNA were detected out respectively;among 966 cases of both HBeAb and HBcAb positive,387 cases(40.1 %)of HBsAg quantitation and 212 cases(21.9%)of PCR-DNA were detected out re-spectively;among 832 cases of all HBV markers negative,6 case (0.7%)of HBsAg quantitation and 4 case (0.5%)of PCR-DNA were detected out respectively,there were statistically significantly differences among them (P < 0.05 ).Conclusion Adopting ELISA for detecting HBV markers,when single HBeAb positive or both HBcAb and HBeAb positive,HBsAg and the viral replica-tion are still be detected out,which needs to conduct further detection in order to avoid the medical risk due to the missed detection.

Objective To investigate the relationship between the genesis of hypertensive disorder complicating pregnancy and neurokinin B (NKB).Methods The serum NKB levels of 229 women including non-pregnant women (n = 62),normal pregnant women (n = 80) and pregnant women with preeclampsia (n=37) or gestational hypertension (n= 50) were examined by enzyme linked immunosorbent assay.The umbilical blood levels of NKB and the NKB mRNA expression in the pregnant women were also tested by fluorescent quantitative reverse transcription-polymerase chain reaction technique.ANOVA and paired t-test were applied.Results (1)The maternal plasma NKB levels of normal pregnant women,patients with gestational hypertension or preeclampsia were significantly higher than that of non-pregnant women,respectively [(28.2±6.6)μg/L,(31.5±5.2)μg/L,(70.5±8.9)μg/L vs(3.2±1.8)μg/L,P＜ 0.01].In preeclampsia,gestational hypertension and normal pregnancy group,the NKB level in umbilical blood [(121.4±9.3)μg/L,(60.5±7.2)μg/L,(40.8±6.3)μg/L] were significantly higher than that of maternal plasma(P＜0.01).(2) The maternal plasma NKB level in gestational hypertension group was higher than that in normal pregnancy group,while the difference had no statistical significance (P＞0.05).The maternal plasma NKB level in preeclampsia group was higher than that in gestational hypertension group and normal pregnancy group (P＜0.01).Forty-eight hours after delivery,the maternal plasma NKB levels significantly decreased in the normal pregnant,gestational hypertension and preeclampsia group [ (14.1±4.2) μg/L,(16.4±3.8) μg/L,(25.4±5.2) μg/L],compared to the values before delivery (P＜0.01).(3) The expression of NKB mRNA in placenta of preeclampsia group was significantly higher than that of gestational hypertension group and normal pregnancy group [(3.8±0.6) × 10-3 vs(1.7±0.4) × 10-3 and (1.6±0.3) × 10-3,P＜0.01].No statistical difference was found in the expression of NKB mRNA in placenta between gestational hypertension group and normal pregnancy group (P＞0.05).Conclusions Elevated NKB level in placenta and maternal or umbilical blood might play an important role in the development of hypertensive disorder complicating pregnancy.

Objective To study the effects of supplemental Dangguibeimukushen pill prescription on the expressions of MMP13 and bFGF in tumor tissues on H22 hepatoma bearing mice.Methods H22 hepatoma bearing mice was employed to carry out anti-tumor experiment in vivo in this study.A total of 60 mice were randomly divided into model group,DDP positive control group,low and high dosage of supplemental Dangguibeimukushen pill treatment group,supplemental Dangguibeimukushen pill combined with DDP group.In each gruop,gastric infusion was performed continuously 14 d.The tumor growth and the general conditions of mice were recorded.After the last administration of gavages orally treatment,all mice were anaesthetized and killed by cervical disloca-tion method to obtain completely tumor tissue for further HE staining measure and detection of MMP13 and bFGF in tumor tissue with the method of RT-qPCR and immunohistochemistry.Results The tumor volume of model group and low dosage of supple-mental Dangguibeimukushen pill treatment group were larger than that of other groups,futhermore,ulceration were found inside tumor,and basic integrity were observed of tumor capsule.However,in all the treatment groups,the tumor volume was relatively smaller,and the capsule of tumor was incomplete,ulceration of different degrees and bleeding when tumor was peeled off.In combi-nation groups,the tumor volume was much smaller,and the tumor had incomplete capsule and less ulceration inside.Pathological observation showed that connective tissue was found inside tumor,cells were sparsely spread and the number of necrosis cancer cells were increased in all treatment groups.RT-qPCR detection showed that the mRNA expression of MMP13 and bFGF in each treat-ment group were decreased comparing with the model group,and the difference was statistically significant(P <0.05).Immunohis-tochemical detection showed that the expression of MMP13 and bFGF in the combined group were less colored and weakly positive expression comparing with the positive group or the pure Chinese medicine treatment group,the difference was statistically signifi-cant(P <0.05).Conclusion Dangguibeimukushen pill could downregulate the expression of MMP13 and bFGF of tumor tissue in H22 hepatocarcinoma xenograft mice at mRNA and protein levels,and played a vital role in inhibiting cancer invasion and angiogen-esis,reducing toxics and side effects and enchancing its efficiency.

Objective To summarize the nursing for acute epididymitis within intermittent catheterization. Methods From August, 2013 to February, 2016, twelve patients suffering acute epididymitis within intermittent catheterization after spinal cord injury were reviewed. Re-sults Epididymitis was cured in all the patients after retention catheterization, psychological nuring, diet and drinking guide, reasonable drug use, etc. Conclusion Acute epididymitis within intermittent catheterization can be controlled by appropriate medication and nursing.