ObjectiveTo evaluate the reliability and validity of Chinese version of the Manager's Activities Scale (MAS). MethodsMAS was administered to 350 nurses and evaluated for the reliability and validity using SPSS17.0 and AMOS 17.0. ResultsThe Cronbach's α of MAS was 0.92 and the testretest reliability was 0.902 and the content validity was 0.96.The correlations between each item and total scale were ranged from 0.720 to 0.823.One factor was extracted by exploratory factor analysis and could explain 56.98％ of the total variance.The confirmatory factor analysis revealed a good fit of the model to the data based on various fit indices (x2/df ratio =1.301,GFI=0.963,AGFI＝0.927,NFI=0.966,IFI＝0.992,TLI=0.987,CFI=0.992,RMSEA=0.039). ConclusionsChinese version of MAS is reliable and valid,and can be used in the research of Chinese nursing leadership.

Objective To analyze the clinical and pathological features and responses to therapy of primary biliary cirrhosis(PBC) and autoimmune hepatitis(AIH) overlap syndrome. Methods Comparison was made between 11 patients with PBC/AIH overlap syndrome, 21 cases with type I AIH and 20 cases with PBC (Scheuer stage Ⅰ and Ⅱ), and the emphases was laid upon the clinical manifestations, pathological features and responses to therapy of the patients with PBC/AIH overlap syndrome. Results No significant differences were found in sex, age and course of diseases among the three groups. In PBC/AIH group, the serum levels of alkaline phosphatase (AKP), ?-glutamyl-transpeptidase (GTP), IgM and the frequency of positive anti-mitochondrial antibodies (AMA) and AMA-M2 antibodies were significantly higher than those in the pure AIH group(P

[ ABSTRACT] AIM:To investigate the effect of DEC1 gene over-expression on the proliferation and invasion abili-ties of human esophageal cancer ECA109 cells.METHODS: ECA109 cells were transfected with plasmid pcDNA3.1 (-)/DEC1 (DEC1 group) or pcDNA3.1 (-) (vector group).The mRNA and protein levels of DEC1, cyclin D1 and MMP-9 were evaluated by real-time PCR and Western blot, respectively.The effects of DEC1 over-expression on the prolif-eration and invasion abilities of the ECA109 cells were evaluated by CCK-8 assay, colony formation assay and Transwell test respectively.RESULTS:The DEC1 expression level in ECA109 cells in DEC1 group was significantly higher than that in vector group (P<0.01), but the levels of MMP9 and cyclin D1 expression were opposite (P<0.01).However, both the proliferation and invasion abilities of ECA109 cells in DEC1 groups decreased significantly as compared with those in vector group (P<0.05).CONCLUSION:The over-expression of DEC1 significantly inhibits the proliferation and invasion of ECA109 cells, which may be involved in the expression of cyclin D1 and MMP9.

Objective To investigate the relationship between the genesis of hypertensive disorder complicating pregnancy and neurokinin B (NKB).Methods The serum NKB levels of 229 women including non-pregnant women (n = 62),normal pregnant women (n = 80) and pregnant women with preeclampsia (n=37) or gestational hypertension (n= 50) were examined by enzyme linked immunosorbent assay.The umbilical blood levels of NKB and the NKB mRNA expression in the pregnant women were also tested by fluorescent quantitative reverse transcription-polymerase chain reaction technique.ANOVA and paired t-test were applied.Results (1)The maternal plasma NKB levels of normal pregnant women,patients with gestational hypertension or preeclampsia were significantly higher than that of non-pregnant women,respectively [(28.2±6.6)μg/L,(31.5±5.2)μg/L,(70.5±8.9)μg/L vs(3.2±1.8)μg/L,P＜ 0.01].In preeclampsia,gestational hypertension and normal pregnancy group,the NKB level in umbilical blood [(121.4±9.3)μg/L,(60.5±7.2)μg/L,(40.8±6.3)μg/L] were significantly higher than that of maternal plasma(P＜0.01).(2) The maternal plasma NKB level in gestational hypertension group was higher than that in normal pregnancy group,while the difference had no statistical significance (P＞0.05).The maternal plasma NKB level in preeclampsia group was higher than that in gestational hypertension group and normal pregnancy group (P＜0.01).Forty-eight hours after delivery,the maternal plasma NKB levels significantly decreased in the normal pregnant,gestational hypertension and preeclampsia group [ (14.1±4.2) μg/L,(16.4±3.8) μg/L,(25.4±5.2) μg/L],compared to the values before delivery (P＜0.01).(3) The expression of NKB mRNA in placenta of preeclampsia group was significantly higher than that of gestational hypertension group and normal pregnancy group [(3.8±0.6) × 10-3 vs(1.7±0.4) × 10-3 and (1.6±0.3) × 10-3,P＜0.01].No statistical difference was found in the expression of NKB mRNA in placenta between gestational hypertension group and normal pregnancy group (P＞0.05).Conclusions Elevated NKB level in placenta and maternal or umbilical blood might play an important role in the development of hypertensive disorder complicating pregnancy.

Objective To investigate the effect of psychological abuse in childhood on the personality of undergraduates with depression as well as its possible mechanism, and thus provide scientific evidence for the intervention of depression in undergraduates. Methods Particinants were 733 undergraduates from a university in Henan Province. They were surveyed with Center for Epidemiological Studies-Depression scale (CES-D), childhood experience of care and abuse questionnaire (CECA. Q) and Eysenck personality theory (EPQ). Results the correlation between maternal antipathy and introversion-extroversion of personality;psychological abuse in childhood had apparently positive correlation with the depression in undergraduates (r = 0. 257, r = 0. 304, r =0. 243, P＜ 0. 01) and that of childhood maternal neglect to psychoticism (β = 0.206, P＜ 0.01) were positive and significant. The standard regression coefficients of paternal neglect to introversion-extroversion (β= -0. 143, P＜0. 01)were negative and significant . The scores of childhood paternal antipathy and maternal antipathy explained that childhood paternal antipathy had indirect effect via adulthood neuroticism on the depression in undergraduates,furthermore, the indirect effect demonstrated 59.8% of the total effect. Conclusion This study suggests that the personality of undergraduates with depression is affected directly by psychological abuse in childhood, and adulthood personality may mediate the relation between childhood psychological abuse and adulthood depression.

Objective To discuss the influence of establishment of special nursing clinic on medical compliance behavior of diabetic patients. Methods We randomly selected outpatients from the endocrine department and special nursing clinic as the control group and the observation group with 100 cases in each group respectively. The condition of medical compliance behavior and blood glucose control was observed and compared in the two groups. Results The patients visiting special nursing clinic were better in medical compliance behavior and blood glucose control. Conclusions Establishment of special nursing clinic for diabetic outpatients can improve the medical compliance behavior, satisfying the requirement for disease knowledge, enhance the shaping of healthy behavior and facilitate the control of disease progress.

Objective To study anxiety and depression among the abused children and their association with psychological resilience.Methods Self-rated questionnaires including the screen questionnaire of child abuse(SQCA),the screen for child anxiety related emotional disorder(SCARED),self-rating depression scale for children(DSRSC),Resilience Scale and General information questionnaire were applied to 447 children.Results (1) The score and rating of SCARED and DSRSC in the group of abused children(respectively(20.39 ± 12.79),(11.65 ± 5.44) and 40.26％,27.27％) was higher than that in control group(respectively(14.74 ± 10.91),(10.18 ± 4.75) and 22.43％,12.97％),the difference was statistically significant (P ＜ 0.01).(2) The score of psychological resilience of abused children in group of anxiety and depression (respectively (80.13 ± 12.82),(74.67 ± 10.71)) was lower than that in control group (respectively (93.91 ± 16.08),(93.50 ± 15.00)),the difference was statistically significant(P＜ 0.01).(3) Emotion control was a significantly negative predictor of anxiety among the abused children.Social assistance,family support and emotion control was a significantly negative predictor of depression among the abused children.Conclusion The abused children have more anxiety and depression problems.psychological resilience is an important predictive variable for anxiety and depression among the abused children.

OBJECTIVE: To study the effect of total flavonoids from Alpinia officinarum on gastrointestinal motility.METHODS: Gastric emptying method was applied to observe the effect of total flavonoids from A.officinarum on gastric emptying of normal mice and sthenic gastric emptying induced by pyridostigmine bromide.Effect of total flavonoids from A.officinarum on gastric smooth muscle of rats was observed through in vitro test and small intestine advancement was used to observe the influence of total flavonoids from A.officinarum on intestinal motility.RESULTS: Total flavonoids from A.officinarum had no significant influence on gastric emptying of normal mice,but distinctly inhibited sthenic gastric emptying induced by pyridostigmine bromide(P

Aim To probe into the best conditions for the establishment of endometriosis model in rats by the orthogonal experiment method.Methods Autotransplantation was used to establish the endometriosis model in rats by the orthogonal experiment method in which four main factors were considered to optimize the condition of model,according to the volume of heterotopic endometrium.Results The para ligament of uterus was the best place for transplanting.Both dissecting muscular layer and injecting estrin played an important role in the establishment of endometriosis model in rats. The value of four factors decreased progressively according to the injection of estrin,the dissection of muscular layer,the direction of endometrium implantation and oestrus.Conclusions By optimizing the condition for the establishment of endometriosis model in rats by orthogonal experiment,the volume of heterotopic endometrium was improved.A technology platform for pharmacodynamics research was thus offered.

Objective To evaluate the relationship between characteristics of cervical cancer at contrast-enhanced ultrasound and prognostic factors.Methods Thirty-five patients with pathologically confirmed cervical cancer were enrolled in this retrospective analysis.All the patients accepted contrast-enhanced ultrasound examination before the surgery.Relationships between the characters of contrast-enhanced ultrasound and the expression of ki-67 antigen(cell proliferation marker),tumor diameter,lymph node metastasis and histologic grade were studied.Results (1)Compared to the surrounding normal tissue, the cervical cancer had statistically significant differences in time to peak and peak intensity(P <0.05),the former was shortened,and the latter was increased.(2 )The ratio of perfusion defect and heterogeneous enhancement were significantly higher in tissues,including the ki-67 positive expression tissue,poorly differentiated or larger tumors (≥ 2.0 cm in diameter)(P < 0.05 ).(3 )Compared to the ki-67 negative expression group,time to peak of the ki-67 positive expression group was shorter and the peak intensity was higher,the differences were statistically significant (P < 0.05 ).(4 )The peak intensity of poorly differentiated cervical cancers was significantly higher than that of the other groups (P <0.05).Conclusions Enhancement patterns and parameters of contrast-enhanced ultrasound were associated with prognosis, which may be useful in noninvasive prediction of the patients with cervical cancers.