Parkinson's disease (PD) is a neurodegenerative disease commonly found in middle-aged and elderly people,and its onset is attributable to both genetic and environmental factors. The gene mutation and oxidative damage of DJ-1 correlate with familial and sporadic PD,respectively. The mechanisms by which DJ-1 gene mutation and oxidative damage lead to PD include oxidative stress,mitochondrial dysfunction and proteasome dysfunction.Furthermore,DJ-1 is a biomarker of sporadic PD and it may become a new potential target of anti-PD drugs.

目的探讨互动式教育对康复治疗系实习生在运动疗法实习期间的学习效果。方法两组学生分别采用传统教学法和互动式教学法带教实习,实习结束,比较两组学生出科测试成绩。结果接受互动式教学法的学生成绩明显优于传统教学法(P<0.01)。结论互动式教学法可提高康复治疗系实习生的教学效果。

Objective To observe the effect of aerobic exercise of different intensities on type Ⅱ collagen,glycosaminoglycan (GAG) and chondrocyte apoptosis in rabbits modeling knee osteoarthritis (OA),so as to explore the preventive effect and its possible mechanism.Methods Twenty healthy New Zealand white rabbits were randomly divided into Groups A,B,C and D,each of 5.Group A was allowed free activity in a cage for 9 weeks.Group B was allowed free activity for 4 weeks,then an OA model was established using papain and confirmed via MRI 1 week later,Another 4 weeks of free activity were then allowed.Groups C and group D were given running training for 20 minutes a day at 0.5 km/h,3 times a week,and then 20 minutes a day at 1.5 km/h,5 days a week on a treadmill for 4 weeks.Nine weeks later,all 4 groups of rabbits were killed and the articular cartilage damage of each group was compared using Mankin scoring,and expression of type Ⅱ collagen,GAG content and chondrocyte apoptosis in the cartilage.Results After the intervention,the average Mankin score,expression of type Ⅱ collagen and GAG content of groups B,C and D were significantly lower than those of group A,and all of those values in group B were significantly lower than those of group D.After 9 weeks the chondrocyte apoptosis rate of group A was significantly lower than that of the other groups,and that of groups C and D was significantly lower than that of group B.Conclusion Aerobic exercise may prevent knee articular cartilage degeneration through inhibiting reduction in the amount of type Ⅱ collagen and GAG in the cartilage matrix.It may be related to decreasing the chondrocyte apoptosis.

Objective To discuss the role of interleukin (IL)-12 in capillary bronchiolitis disease.Methods Fifty-nine cases of capillary bronchiolitis children under 2 years old were enrolled as the bronchiolitis group Ⅰ(n =28) and bronchiolitis group Ⅱ(n =31),36 cases of children with bronchopneumonia of the same age and 31 cases of children suffered from non-infectious diseases such as hernia and renal calculus of the same age were enrolled as bronchopneumonia control group and normal control group respectively.The peripheral blood IL-12 levels of four groups were detected by enzyme-linked immunosorbent assay.Results The levels of the peripheral blood IL-12 in bronchiolitis group Ⅰ,bronchiolitis group Ⅱ,bronchopneumonia control group and normal control group were (34.72±7.96) pg/ml,(55.30 ±6.72) pg/ml,(56.79±10.36) pg/ml and (61.23 ± 11.51) pg/ml respectively.The level of the peripheral blood IL-12 in bronchiolitis group I was significantly lower than that in bronchiolitis group Ⅱ,bronchopneumonia control group and normal control group respectively (P ＜0.05),and the level of the peripheral blood IL-12 in bronchiolitis group ⅡⅡ was significantly lower than that in bronchopneumonia control group and normal control group respectively (P ＜ 0.05),but there was no significant difference between bronchopneumonia control group and normal control group (P ＞ 0.05).Conclusion The level of IL-12 is one of the important factors for bronchiolitis disease,the reducing of serum level of IL-12 in children who have a high risk factor of capillary bronchiolitis is more obvious.

AIM: To assess the therapeutic effects and mechanisms of Shenhuang Jinkang Tablet(Radix et rhi-zoma salviae miltiorrhizae,Radix astragali,Rhizoma atractylodis macrocephalae,Rhizoma curcumae longae,Radix angelicae sinensis,Concha ostreae,Concha ostreae praeparatum) on cervical spondylosis in the rat model.METH-ODS: Sprague-Dawley rats were divided into 6 groups at random.The cervical spondylosis rat model was induced,and then administered with normal saline,Shenhuang Jingkang Tablet(500 mg/kg,1 000 mg/kg,2 000 mg/kg) and Jingfukang,respectively.To observe the movement,sensation and morphological change in rat model.The contents of SOD,MDA,IL-6 in serum were tested.RESULTS: As compared with the control group,the activity of cervical part was significantly improved,the oppression was relieved.The average functional angle increased in Shenhuang Jingkang Tablet groups.Difference was significant(P

Objective To study whether the early continuous passive motion (CPM) can prevent the degeneration of articulation after the articular cartilage was experimentally defected, and the effect of the different durations of continuous passive motion therapy. Methods Fifteen New Zealand rabbits were used to establish the model of full-thickness defects of bilateral knee articular cartilage by operation. Then all the animals were divided into groups A, B and C: Group A served as the control group, and groups B and C served as the observation groups and were administered with 2 hours of continuous passive motion a day (group B) and 8 hours of continuous passive motion a day, respectively. All rabbits were sacrificed at the 12th week after operation, and their articular cartilage were observed. The cartilage and synovium were obtained to have the hematoxylin and eosin stain and immunohistochemistry stain. After that, the pathological assessment and calculation of the ratio of MMP-3 positive cells were conducted and compared among groups. Results Group C was lower than groups A and B in terms of the scores with cartilage pathological assessment and the ratio of MMP-3 positive cells(P

4.0 to Candida albicans in the 150 mg/L of available iodine concentration for 1 min,or 300 mg/L of available iodine concentration for 0.5 min,respectively.After being stored at 37 ℃ for 90 days or exposured 6 h per-day in consecutive 6 days,the available iodine content of the stock solution and quality of the sample slightly changed.The germicidal logarithmic value was more than 1.00 to the forearm skin natural bacteria when smeared 1 time for 1 min.CONCLUSIONS The germicidal effects of Shu-Jin iodine disinfection solution is good and stable.

Objective To study the sensitivity and specificity of the Chinese eating assessment tool (EAT-10) in screening acute stroke patients for oropharyngeal dysphagia (OD).Methods A total of 130 inpatients with acute stroke were screened using the Chinese EAT-10.On the same day they were also screened using the gold standard technique for diagnosing dysphasia-videofluoroscopy.A receiver operating characteristics (ROC) curve was developed to study EAT-10's sensitivity and specificity.A Youden index,positive predictive value (PPV),negative predictive value (NPV),and positive and negative likelihood ratios (LHR+ and LHR) were quantified.Results According to the ROC curve,a cut-off point of 1 (EAT-10 score≥ 1) gave the best sensitivity (77.9％),the highest NPV (73.2％),with 66.1％ specificity,71.6％ PPV,2.30 LHR+ and 0.33 LHR in screening for OD.The test-retest reliability was above 0.7.An investigator consistency reliability test showed good repeatability,and the consistency between each item and the mean total score was high.Conclusion The Chinese EAT-10 has good test-retest reliability and investigator consistency.The optimal cut-off point is 1,with good sensitivity and NPV at scores ≥ 1.The test can be recommended as a screening tool for OD in acute stroke patients.

To assess the level of health skills relating to infectious diseases among residents in Beijing and develop more effective strategies for controlling infectious diseases.We conducted a multistage sampling,retrospective cross-sectional survey of residents aged 18 and above from 6 districts by selfadministered and anonymous questionnaires in January 2011.The questionnaires consisted of health skills relating to infectious diseases.A total of 13 042 respondents completed the survey.The proportions of people with the skills for acquiring knowledge about infectious diseases,being able to read medicinal package insert,being able to read brochures with regard to basic knowledge about infectious diseases, being able to read laboratory test report andproper use of thermometer were 57.7％,71.0％,70.2％,47.9％ and 76.8％ respectively.The levels of health skills were different among different populations (P ＜0.01).A low level of health skills was observed among the poorly-educated people and elders.Therefore measures such as health education and skills training about infectious diseases should be taken to improve the health skills.

Objective To explore the clinical features,efficacy and prognosis of different treatments for children with achalasia of cardia(AC).Methods In this retrospective study,the clinical features,laboratory examination and treatment of 36 children with AC who had been admitted to Department of Gastroenterology,Beijing Children's Hospital,Capital Medical University from August 2006 to September 2015 were reviewed,and the efficacy and prognosis of different treatments were compared.The symptoms of the children were graded using the AC clinical symptom score(Eckardt score),and the Eckardt score ≤ 3 scores was defined as the effective treatment.SPSS 19.0 statistical software was used to analyze the data,and P<0.05 for the difference was statistically significant.Results Thirty-six children with AC included 24 boys and 12 girls.Ages ranged from 1.4 to 15.5 years old,with a mean age of(10.0±3.4)years old.Course of disease ranged from 1 month to 9 years,with a mean course of 0.5(0.2,3.0)years.In the 36 children,33 cases(91.7％)had vomiting,23 cases(63.9％)had dysphagia,16 cases(44.4％)had weight loss,and 9 cases(25.0％)had chest pain.The effective rates of treatment in surgical treatment group and drug treatment group were 100.0％(13/13 cases)and 71.4％(5/7 cases),respectively in 3 months,and there was no significant difference between the 2 groups(P=0.111).The effective rates of treatment were 100.0％(13/13 cases)and 50.0％(3/6 cases),respectively in 6 months,and the difference was statistically significant between the 2 groups(P=0.021).Within 12 months,there was no recurrence in surgical treatment group and the effective rate was 100.0％.Children in drug treatment group had 1 case who stopped taking medicine,while the other children received surgical treatment in other hospitals due to poor drug treatment.Conclusions Drug and surgical treatment of AC both have good short-term effect,however,the medium and long-term efficacy of surgical treatment is higher than that of drug treatment in children.Symptomatic relief is more stable,and symptom is not easy to relapse for the children with surgical treatment.