Objective To evaluate the value of the peripheral capillary fasting plasma glucose examined by glucometer on screening of type 2 diabetes.Methods With the proportion stratified group sampling,all residents aged 15-75 years in Wuqing district were selected and detected on their capillary fasting plasma glucose(CFPG) by clucometer,and those whose CFPG was higher than 5.0mmol/L should have a 2-hour postprandial glucose(2 h PG) test.Data were analyzed by SPSS 11.5 software.Results The detection rates of diabetes,impaired glucose tolerance and impaired fasting blood glucose were 42.83%,27.88% and 3.74%,respectively.Making two ROC curve with test variable(FPG) and state variables(2 h PG≥11.1 mmol/L and 7.8 mmol/L≤2 h PG≤11.0 mmol/L),the areas under the curve were 0.862 and 0.405,respectively.Conclusion It is suggested to use the sensitivity standard that nears to 99% FPG≥5.0 mmol/L to screen diabetes.

Aim To investigate the inhibitory effects of urocortin2 (UCN2) on ventral tegmental area (VTA) nervous activity of morphine addiction rats and the mechanism. Methods Morphine addiction rats and the microiontophoresis method were used to observe the effects of UCN2 on VTA neuron′s spontaneous dis-charge changing rule, as well as the inhibitory effects of UCN2 on DA neuron′s cluster spontaneous dis-charge, to identify UCN2 and DA on the same VTA neuron. Morever, the inhibitor of corticotropin-regula-ting factor′s receptor ( CRF-2 R ) and the blocker of protein kinase A ( PKA ) , AST-2 B and H89 , were used to investigate the effects of UCN2 on VTA neuron′s of morphine addiction rats. Results UCN2 could inhibit the firing rate 82% (31/38) of the tested VTA neuron ( P<0. 01 ) , the discharge frequency changed from (20. 89 ± 2. 86) Hz to (13. 66 ± 3. 93) Hz (P<0. 01). Further, the inhibitor of PKA, AST-2B and H89 could ablolish the inhibitory effects of UCN2 . Morever, the excitatory firing of VTA neurons was at-tenuated by addition of UCN2 , while AST application could inhibit the UCN2′s inhibitory effects. Conclu-sion UCN2 could regulate the effects of VTA via PKA pathway and may thereby contribute to the improvement of drug addiction.

Aim To investigate the effects of endocrinal petptide urocortin on subthalamic nucleus (STN) neuron''s discharge, also observe the convergence effect of UCN with dopamine (DA) and glutamate (GLU), so as to understand the regulation effects of UCN and its mechanism in Parkinson''s disease (PD).Methods Forty Sprague-Dawley rats were used in this experiment.Nerve electrophysiology method-microiontophoresis was used to observe the effects of UCN on STN neuron firing rates and firing wave.Astressin (AST, the blocker of CRF receptor 2), protein kinase A (PKA) were used to observe the effects of UCN whether via CRF-2R and PKA signal pathway.Moreover, given UCN during the period of DA and GLU, the effects of UCN on DA and GLU in STN neurons were determined.Results During the period of using the UCN, UCN could inhibit the firing rate of 82％ (27/33) STN neuron (P<0.01), and the firing discharge rates were reduced from(3.65±0.27)Hz to (2.05±0.33) Hz (P<0.01).However, the inhibitory effects of UCN in STN could be antagonized by AST.Given UCN during the period of microiontophoresis of inhibitory neurotransmitter (DA) and excited neurotransmitter (GLU), UCN could enhance the effects of DA and attenuate the excitatory effects of GLU (P<0.01).Conclusion UCN and GLU/DA in STN, UCN play inhibitory and regulated effects on STN neurotransmitters(DA and GLU)via CRF-2 receptor and PKA signal pathway.

AIM: To assess the therapeutic effects and mechanisms of Shenhuang Jinkang Tablet(Radix et rhi-zoma salviae miltiorrhizae,Radix astragali,Rhizoma atractylodis macrocephalae,Rhizoma curcumae longae,Radix angelicae sinensis,Concha ostreae,Concha ostreae praeparatum) on cervical spondylosis in the rat model.METH-ODS: Sprague-Dawley rats were divided into 6 groups at random.The cervical spondylosis rat model was induced,and then administered with normal saline,Shenhuang Jingkang Tablet(500 mg/kg,1 000 mg/kg,2 000 mg/kg) and Jingfukang,respectively.To observe the movement,sensation and morphological change in rat model.The contents of SOD,MDA,IL-6 in serum were tested.RESULTS: As compared with the control group,the activity of cervical part was significantly improved,the oppression was relieved.The average functional angle increased in Shenhuang Jingkang Tablet groups.Difference was significant(P

Aim The goal is to make a further comprehension of the pathogenesis of Parkinsons disease (PD) and hence add further knowledge for PD diagnosis by observing the functions of nitric oxide (NO) in striatum (STR). Method Microelectrodes were used to observe the effects of sodium nitroprusside (SNP), L-glutamic acid (GLU) and ?-aminobutyric acid (GABA) on STR neurons' spontaneous firing rates, and at the same time, we observed the effects of NO on GLU and GABA. Results 77% (51/66) of the tested neurons were excited by SNP, NO synthase inhibitor L-NAME antagonized the excitatory effect of SNP. Duringthe periods of microelectrophoresis GLU and GABA, SNP amplified the excitatory effect of GLU and weakened the inhibitory effect of GABA. L-NAME antagonized the excitatory effect of GLU.Conclusion These results demonstrated clearly that NO, GLU and GABA functions might converge in the same STR neurons. NO and GLU functions are excitatory whereas GABA function is inhibitory on the firing activities in STR neurons.

Objective To explore the way of continuing education training of standardized management of community nurses in Tianjin.Methods A survey was conducted with self-designed questionnaires for 462 community nurses who attended the continuing education training,and behavioral event interview was carried out for 6 directors of district health bureau who participated in the continuing education.Results The acquirements and problems of continuing education training of standardized management in Tianjin community nurses were concluded from the data analysis.Conclusions It is necessary to give continuing education training of standardized management for Tianjin community nurses.We should improve the evaluation and feedback system,attach importance to management of teaching time and teaching manner.

In this paper, three higher content of flavonol glycosides from the stems and leaves of Tribulus terrestris L. were determined by reversed phase HPLC. The results showed that there was good liner relationship between the peak areas and the sample concentration at the ranges of 0.011 2~0.280 0 μg (r=0.999 6), 0.064 8~2.592 0 μg (r=0.999 8) and 0.018 4~0.460 0 μg (r=0.999 8) for isorhamnetin-3-O-β-D-gentiobioside-7-O-β-D-glucoside, quercetin-3-O-β-D-gentiobioside and isorhamnetin-3-O-β-D-gentiobioside respectively. The verage recovery rates (n=9) of three flavonol glycosides compounds were 100.15%(RSD=1.32%), 100.02% (RSD=1.14%), 99.77% (RSD=1.16%), respectively. This method is considered to be simple, accurate, stable, good precision and reproducibility, which is available for the quality control and evaluation of T. terrestris L.

Objective To probe into the clinical value of the whole pelvis internal organs joint excision in locally advanced cervical carcinoma. Methods Four cases of local cervical carcinoma patients who have been implemented the peculiar operation from April 1997 to April 2001 were analyzed. Results The operation progressed well and the time of surviving was 4-41 months, 16.3 months in life cycle on average. 4 patients died of intestinal obstruction, vagina-small intestine atrophy and the pain of pelvis which resulted in a hunger strike and lung metastasis. Conclusion Implement the operation to those patients whose rectum and bladder were invaded simultaneously can lengthen a patient's life cycle and improve the quality of surviving. To grasp the operation target, standardize the step and improve the treatment method before and after operation were necessary.

Aim To investigate the effects of Rehmannia glutinosa oligosaccharides(ROS)on the damage induced by glutamate in hippocampal neurons.Methods The neurons isolated from hippocampus in new born SD rats were cultured for 7~9 days,which were specifically stained with NSE and then randomly divided into four groups:(Ⅰ)Normal cultures(control);(Ⅱ)ROS control cultures;(Ⅲ)Glutamate-exposed control cultures;(Ⅳ)Glutamate-exposed cultures pretreated with ROS.The neurons morphology was observed under inverted microscope;cell viability was assayed by MTT staining;LDH release was detected with chromatometry and flow cytometric analysis for identification and quantification of cell apoptosis.Results Compared with normal group,after exposure of glutamate for 24 h,the viability of neurons was decreased,LDH release and cell apoptosis were increased(P

This study was aimed to establish a method for the content determination of three components in Tribulus terrestris L., in order to compare index components of different growing periods, production places and medical parts for the scientific explanation of the harvesting time and growing places. Contents of three effective components inTribulus terrestris L., which were quercetin-3-O-β-D-gentiobioside, isorhamnetin-3-O-β-D-gentiobioside and Terrestrinone A2, were determined by HPLC. The results showed that contents of three evaluation indexes all gradually increased until June and then started reducing. The contents in all evaluated indexes were in the order of the stem and leaf > fruit > root. And the content in the root was below the detection limit. The content indexes ofTribulus terrestris L. of the Inner Mongolia were much higher than that of other regions. It was followed byShandong,Hebei andShanxi province. It was concluded that the method was simple and accurate with good repeatability and stability, which was suitable for quality control ofTribulus terrestris L.