Objective To evaluate the value of the peripheral capillary fasting plasma glucose examined by glucometer on screening of type 2 diabetes.Methods With the proportion stratified group sampling,all residents aged 15-75 years in Wuqing district were selected and detected on their capillary fasting plasma glucose(CFPG) by clucometer,and those whose CFPG was higher than 5.0mmol/L should have a 2-hour postprandial glucose(2 h PG) test.Data were analyzed by SPSS 11.5 software.Results The detection rates of diabetes,impaired glucose tolerance and impaired fasting blood glucose were 42.83%,27.88% and 3.74%,respectively.Making two ROC curve with test variable(FPG) and state variables(2 h PG≥11.1 mmol/L and 7.8 mmol/L≤2 h PG≤11.0 mmol/L),the areas under the curve were 0.862 and 0.405,respectively.Conclusion It is suggested to use the sensitivity standard that nears to 99% FPG≥5.0 mmol/L to screen diabetes.

Parkinson's disease (PD) is a neurodegenerative disease commonly found in middle-aged and elderly people,and its onset is attributable to both genetic and environmental factors. The gene mutation and oxidative damage of DJ-1 correlate with familial and sporadic PD,respectively. The mechanisms by which DJ-1 gene mutation and oxidative damage lead to PD include oxidative stress,mitochondrial dysfunction and proteasome dysfunction.Furthermore,DJ-1 is a biomarker of sporadic PD and it may become a new potential target of anti-PD drugs.

Objective To determine the risk factors for coronary artery lesions (CALs) in patients with Kawasaki disease (KD).Methods The clinical records of 2331 patients with KD from January 2005 to December 2014 in Beijing Children's Hospital,Capital Medical University were retrospectively analyzed.The relationship between the following factors and CALs was analyzed by univariate and multivariable Logistic regression analysis:age,gender,incomplete KD,total fever duration,intravenous immunoglobulin(IVIG) treatment resistance,white blood cell count,C-reactive protein (CRP),platelet count,sodium and albumin.Results The incidence of CALs was 36.0％(840/2331 cases).Univariate Logistic regression analysis indicated that male patients,incomplete KD,total fever duration ≥10days,IVIG treatment resistance,CRP＞100mg/L,platelet count＞300×109/L and albumin＜35 g/L were associated with CALs (all P＜0.05).Multivariable Logistic regression analysis identified that male patients (OR=1.698,95％ CI 1.383-2.084,P＜0.001),incomplete KD (OR=2.730,95％ CI 2.121-3.515,P＜0.001),total fever duration ≥10 days (OR=2.556,95％ CI 1.975-3.307,P＜0.001),CRP＞100 mg/L (OR=1.556,95％ CI 1.274-1.900,P＜0.001) and albumin＜35 g/L (OR=1.665,95％ CI 1.323-2.096,P＜0.001) were the independent risk factors for CALs.Conclusions The main damage in patients with KD is CALs.The male children with KD,incomplete KD,total fever duration ≥10 days,CRP＞100 mg/L and albumin＜35 g/L were prone to CALs.

Objective:To evaluate the effects of Er,Cr:YSGG laser on the root surface of periodontally involved teeth and healthy teeth,concerning the microstructure and the roughness.Methods:Eight freshly extracted teeth due to severe periodontitis and eight freshly extracted teeth due to orthodontic rea-sons or being third molar were chosen in this study.The root surface of each tooth was divided into four areas,and received four treatment methods:no treatment (control group );root planing with Gracey scaler for 30 seconds;irradiation by the lower power Er,Cr:YSGG laser;irradiation by the higher power Er,Cr:YSGG laser.Four periodontally involved teeth and four healthy teeth were used for the evaluation of microstructure using scanning electron microscope (SEM).The other four periodontitis teeth and four healthy teeth were used for the evaluation of roughness (Ra value )using 3 D profiler.Results:Smear layer was found on the teeth scaled by Gracey scaler,while the teeth irradiated by Er,Cr:YSGG laser demonstrated a melting surface with less smear layer.In the periodontitis teeth irradiated by the higher power,opening dentinal tubules could be observed.For the periodontally involved teeth,the Ra values of groups 1 to 4 were (237.4 ±20.0)nm,(1 35.7 ±1 1 .9)nm (P=0.01 ),(463.6 ±49.3)nm (P<0.001 )and (486.0 ±59.0)nm (P<0.001 )respectively.For the healthy teeth,the Ra values of groups 1 to 4 were (1 91 .4 ±44.5)nm,(1 31 .6 ±21 .5)nm (P=0.482),(463.7 ±34.6)nm (P<0.001 )and (470.3 ±1 21 .3)nm (P<0.001 )respectively.Conclusion:Er,Cr:YSGG laser can affect the microstructure of the cementum of the periodontitis teeth and healthy teeth.Irradiation by the Er,Cr:YSGG laser resulted in a melting surface with less smear layer and increased the roughness in the surface of root.

Aim To establish a rat aortic calcification sub-chronic model induced by vitamin D3 and nicotine.Methods The vascular calcification model was produced by vitamin D3(300 kIU?kg-1,im) plus nicotine(25 mg?kg-1,5 ml?kg-1,po).Rat body weight and blood pressure were monitored on a regular basis.Ratio of heart to body weight,plasma calcium,plasma phosphate content,vascular calcium content,Von Kossa staining,activity of alkaline phosphatase(ALP) and 45Ca deposition in aorta were measured as the index of calcification at the 8th and the 16th week.Results Compared with control group,the VDN group rats showed higher values of blood pressure from the 3rd week(all P

目的探讨互动式教育对康复治疗系实习生在运动疗法实习期间的学习效果。方法两组学生分别采用传统教学法和互动式教学法带教实习,实习结束,比较两组学生出科测试成绩。结果接受互动式教学法的学生成绩明显优于传统教学法(P<0.01)。结论互动式教学法可提高康复治疗系实习生的教学效果。

Background:Chronic atrophic gastritis(CAG)is a kind of chronic gastritis with atrophic changes of gastric mucosa. The studies on peripheral blood biomarkers in CAG are rare. Aims:To investigate the methylation of peripheral blood CpG sites in Runx3 gene promoter region in CAG patients. Methods:Eighty-two mild CAG patients,73 moderate to severe CAG patients from June 2013 to May 2014 at Daqing Oilfield General Hospital were enrolled,and 45 patients with normal gastric mucosa were served as controls. The methylation of CpG sites in Runx3 gene promoter region was measured by MALDI-TOF-MS. mRNA expression of Runx3 was determined by fluorescent quantitative PCR,and the protein expression of Runx3 was determined by Western blotting. Results:Compared with the control group and mild CAG group,methylation levels of CpG13,CpG14 and CpG15 sites in Runx3 gene promoter region were significantly increased in moderate to severe CAG group(P < 0. 05),the mRNA and protein expressions of Runx3 were significantly decreased( P < 0. 05). No significant differences in methylation of CpG sites in Runx3 gene promoter region and mRNA and protein expressions of Runx3 were found between mild CAG group and control group( P >0. 05 ). Conclusions:The hypermethylation of peripheral blood CpG13,CpG14 and CpG15 sites in Runx3 gene promoter region can inhibit the expression of Runx3 in CAG patients,and can be used potentially as the biomarker for clinical staging of CAG.

Objective To observe the effect of aerobic exercise of different intensities on type Ⅱ collagen,glycosaminoglycan (GAG) and chondrocyte apoptosis in rabbits modeling knee osteoarthritis (OA),so as to explore the preventive effect and its possible mechanism.Methods Twenty healthy New Zealand white rabbits were randomly divided into Groups A,B,C and D,each of 5.Group A was allowed free activity in a cage for 9 weeks.Group B was allowed free activity for 4 weeks,then an OA model was established using papain and confirmed via MRI 1 week later,Another 4 weeks of free activity were then allowed.Groups C and group D were given running training for 20 minutes a day at 0.5 km/h,3 times a week,and then 20 minutes a day at 1.5 km/h,5 days a week on a treadmill for 4 weeks.Nine weeks later,all 4 groups of rabbits were killed and the articular cartilage damage of each group was compared using Mankin scoring,and expression of type Ⅱ collagen,GAG content and chondrocyte apoptosis in the cartilage.Results After the intervention,the average Mankin score,expression of type Ⅱ collagen and GAG content of groups B,C and D were significantly lower than those of group A,and all of those values in group B were significantly lower than those of group D.After 9 weeks the chondrocyte apoptosis rate of group A was significantly lower than that of the other groups,and that of groups C and D was significantly lower than that of group B.Conclusion Aerobic exercise may prevent knee articular cartilage degeneration through inhibiting reduction in the amount of type Ⅱ collagen and GAG in the cartilage matrix.It may be related to decreasing the chondrocyte apoptosis.

Clostridium difficile is a gram-positive,obligate anaerobic bacillus,which is one of the most common pathogenic bacteria of antibiotic associated diarrhea,and can cause Clostridium difficile-associated diarrhea.In recent years,the incidence of Clostridium difficile infection has increased significantly in the world with the excessive use of broad-spectrum antibiotics,the increase of strains resistance,and the emergence of hypervirulent strains.This paper presents a brief review on research progress of Clostridium difficile-associated diarrhea.

A multi-stage stratified sampling method and a self-designed questionnaire were used to evaluate the prevalence of conjunctivitis symptoms and associated factors among the Beijing residents.In total,12 890 residents aged over 18 years were recruited from 6 districts.The reported prevalence of conjunctivitis symptom at the last years was 1.60％.Self-reported health condition and personal health behaviors were risk factors associated with conjunctivitis among adult Beijing residents.No significant difference existed between different districts,age groups,educational levels,gender and races.Conjunctivitis symptom among Beijing residents was related with health condition and health behaviors.More measures should be taken to cultivate the health model of behaviors.