Objective To investigate the relationship between omentin-1 and Coronary heart disease in different glucose tolerance populations. Methods Serum omentin-1 levels were determined by ELISA in 143 subjects with normal glucose tolerance,impaired glucose tolerance with CHD, type 2 diabetes mellitus with CHD. The relationships between omentin-1 and waist- to-hip ratio, body mass index (BMI), blood Glucose, serum insulin,HOMA-IR and blood fat were also explored.Coronary artery stenosis was screnned by coronary interventional angiography and assessed by Gensini scoring system. Results Serum omentin-1 levels were all lower in T2DM and IGT subjects than those in control subjects (P<0.01). Serum omentin-1 was correlated positively with high density lipoprotein-cholesterol (P < 0.01),and inversely with body mass index,waist-hip ratio,diastolic blood pressure, triglyceride,fasting blood glucose,fasting insulin,HbA1c,[w1]and insulin resistance(HOMA-IR)(P<0.05 or P <0.01).Omentin-1 levels was negative related with Gensini coronary artery score (P < 0.01). Multiple linear regression showed body mass index,HOMA-IR,diastolic blood pressure were independently associated with omentin-1 levels (P<0.05 or P<0.01). Conclusions Serum omentin-1 levels, associated with fat,insulin sensitivity, are likely to play an important role in insulin resistance and the development of CHD in populations with different glucose tolerance.

Objectives To explore the influencing factors of delirium in elderly patients with mechanical ventilation in ICU. Methods Using a self-designed questionnaire,the investigation was done among 210 patients undergoing mechanical ventilation to evaluate the delirium condition and look into the influence factors of delirium.Results 112 cases(53.3%)of the elderly patients were affected with delirium.The major influencing factors included duration of mechanical ventilation,acute physiology and chronic health evaluation(APACHEⅡ),hypoxia,sleep deprivation.Conclusion The incidence of delirium is higher among the elderly patients with mechanical ventilation.As for nursing strategy,providence of family nursing,close observation of the disease changes, enhanced respiratory function and practical orientation cognitive training are important for the reduction of the incidence of delirium in the elderly patients with mechanical ventilation.

OBJECTIVE To evaluate the influence of three high-level chemical disinfectants for sterilization on cauterization of dental instruments.METHODS The measuring methods for disinfectants on cauterization of metal instruments,and usage indications for disinfectants followed Technical Standard for Disinfection published in 2002 by the Ministry of Health.Fourteen kinds of small instruments(grouped by 4 different metal materials) were analyzed on receipt and after sterilization by three high-level disinfectants(2% glutaraldehyde,chlorine dioxide, and symclosene),using cauterization rate.RESULTS Three high-level disinfectants had different cauterization rates,the cauterization rate was affected by concentration of disinfectant,sterilizing time,and morphology of instruments.The cauterization of chlorine dioxide was the strongest,followed by symclosene(trichloroisocyanuric acid),and then 2% glutaraldehyde.CONCLUSIONS In clinical sterilization,different materials of small instruments need proper disinfectants,concentration and sterilizing time for decreasing their cauterization.

Objective To investigate the mechanism of anticoagulant proteins defect in patients with unexplained miscarriage. Methods Fifty-seven patients with a history of unexplained abortion in Peking union hospital during may. 1999 to April 2000. were tested for protein S, protein C, and antithrombin(AT)Ⅲ, activated protein C resistance(APC-R).The control group consisted of 50 healthy women with a history of normal pregnancy and delivery. Blood samples were taken for measuring protein S, protein C, AT-Ⅲ and APC-R. Patients with APC-R positive were measured for FV Leiden gene mutation by PCR-RFLP method. Results Of the 57 patients, there were 21.1%?1.8% and 8.8% with protein S?protein C and AT-Ⅲ deficiency respectively. For APC-R 22.8% of the 57 patients were positive .In control group, 4.0% were protein S deficiency; None was protein C and AT-Ⅲ deficiency ; 6.0% were positive for APC-R .No FV Leiden gene mutation was found in all the patients with APC-R positive .The incidence of anticoagulant proteins defect is higher in late spontaneous abortion group than that in early abortion group. Conclusions This study demonstrate that anticoagulant protein defects may be associated with pregnancy loss, especially the late spontaneous abortion.

Objective To compare the therapeutic efficacy and safety of ultrasound-guided thrombin injection (UGTI) combined with saline injection and ultrasound-guided compression repair (UGCR) for upper limb pseudoaneurysm (PSA). Methods The clinical features and therapeutic efficacy of UGCR and UGTI for PSA of the upper extremities were retrospectively assessed after catheterization through radial artery. When UGCR was conducted, PSA, its communicating emissaries with other arteries were first identified with color Doppler, and then direct manual compression over the aneurysm for 30 minutes until complete thrombosis was observed. When UGCR failed, a 20-22G needle was inserted into PSA and saline was injected with continuous color Doppler guidance to confirm that the tip of the needle was in the aneurysm. Then thrombin was injected into the aneurysmal sac. All the patients were followed-up clinically and with ultrasound 24h or 14-30 days after the treatments. Results For the 14 patients with upper limb PSA who received UGCR for the first time, 5 were cured with a success rate of 35.7%. Nine cases underwent repeated UGCR treatment, and it was successful in four patients. The overall success rate for UGCR was 64.3% (9/14). All the five patients in whom UGCR failed were successfully cured when UGTI was conducted. In four cases it was successful after the first UGTI, and only one patient received repeated UGTI treatment. The total success rate for UGTI was 100% (5/5). No obvious adverse effect and recurrence were found after follow-up for 14-30 days in all patients. Conclusions Both UGCR and UGTI are safe, effective, and expedient treatment for patients with PSA in the upper extremity. UGTI is the preferred method for its simplicity, high success rate and avoidance of possible distal arterial embolism.

Objective To explore the relationship between obstructive sleep apnea syndrome(OSAS)and the left ventricular diastolic dysfunction.Methods 20 consecutive newly diagnosed middle-aged OSAS patients(OSAS group)with neither controllable factors nor conditions affecting left ventricular diastolic function and 15 healthy control subjects(control group)were examined with echocardiography.Results The OSAS group exhibited left ventricular diastolic dysfunction compared with the control group,including increase of isovolumic relaxation time(P<0.05)and mitral deceleration time(P<0.05),decrease of E/A.There was no difference between tow groups in interventricular systolic thickness,posterior wall thickness,left ventricular systolic diameter,left ventricular diastolic diameter and others.Conclusion OSAS may be related with the left ventricular diastolic dysfunction.

Objective To investigate the expression of MIF in PBMC, sera and lacrimal fluid samples in patients with AD, and study the diagnostic significance of MIF in AD. Methods Forty-three AD patients (11 mild AD patients,23 moderate AD patients, 9 severe AD patients classfied by SCORAD index)and 31 age- and sex-matched healthy control subjects were recruited. Real-time RT-PCR was employed to analyze the expression of MIF mRNA in PBMC. ELISA was performed to detect the concentrations of MIF in sera and lacrimal fluid samples. Results AD patients had significantly higher levels of MIF mRNA in PBMC than normal controls [7.46 (3.38-8.90) vs 1.67 ( 1.24-2.45 ), Z=-6.141, P ＜ 0.05]. The concentrations of MIF in sera and lacrimal fluid samples in AD patients were also markedly higher than those of normal controls [serum 36. 32( 11.89-43.80) μg/L vs 7.89(6.13-9.54) μg/L, Z = -6.180,P <0.05; lacrimal fluid 12.66(2.01-20.12) μg/L vs 0.85(0.77-1.06) μg/L, Z = -4.118,P <0.05]. MIF mRNA levels were 2.35 ( 2.12-2.49 ) , 7.83 ( 6. 54-8.90 ) and 8.76 ( 8.22-9.73 ) in mild, moderate and severe AD respectively, and the expression was higher in moderate and severe AD than in normal controls (Z= -6.237, -4.520,P ＜0.05). MIF serum concentrations were 8.98(7.90-10.51) μg/L, 36.50 (29.78-43.23) μg/L and 45.70(41.27-48. 84) μg/L in mild, moderate and severe AD respectively, and the differences were significant in moderate and severe AD compared to normal controls ( Z = - 6.238,- 4.521, P < 0.05 ). The lacrimal fluid MIF oncentrations were 1.10 ( 0.83-1.35 ) μg/L, 12.66 ( 9.76-15.87) μg/L and 24. 65 ( 19.29-30.94) μg/L in mild, moderate and severe AD respectively. Similarly,they increased significantly in moderate and severe AD compared to normal controls (Z = -4.062,- 3.372, P ＜ 0.05 ). In moderate and severe AD, MIF mRNA levels in PBMCs and MIF concentrations in sera and lacrimal fluid samples were all positively correlated with the severity of AD ( r = 0.395, 0.404,0.515, P < 0.05 ). Conclusions The expression of MIF in PBMCs, sera and lacrimal fluid samples is higher in different course of AD. MIF can serve as a useful laboratory parameter for evaluation of AD activity and severity.

Glycosyltransferases (GTs) catalyze the transfer of a sugar residue of an activated sugar donor to an acceptor molecule. Many families 1 GTs utilize an uridine diphosphate (UDP) activated sugar as donor in the glycosylation reaction, and most of these belong to a group of GTs referred to as the UGTs. The relationship between the degree of amino acid sequence identity and substrate specificity of the plant UGTs is highly complicated, and the prediction of substrate specificity based on phylogenetic analyses need to be improved by more biochemical characterization. This review summarizes the three dimensional structures of plant UGTs published in the Protein Data Bank (PDB), including the detailed substrate interactions with the sugar and receptor binding pockets and mutational analyses of some critical amino acids. It will be helpful for biochemical characterization the substrate specificity of the individual UGT, and lay the foundation for the enzymatic and genetic manipulation of plant UGTs in the future.
Amino Acid Sequence ; Glycosylation ; Glycosyltransferases ; chemistry ; Phylogeny ; Plant Proteins ; chemistry ; Plants ; enzymology ; Protein Structure, Tertiary ; Substrate Specificity ; Uridine Diphosphate ; chemistry

Objective To establish a feasible evaluation index and method to identify composition of re-maining metal particles on ferrochrome kitchen knife. Methods The small samples of remaining metal particles were rubbed fromthe knives using filter paper. The composition of remaining metal particles was detected by scanning electron microscopy and energy dispersive X-ray spectrometer (SE m-EDX ) and GSR particle analysis function, using mathematical methods to calculate the ratio (relative amount) of Fe and Cr in remaining metal particles. Results The ratio (relative amount) of Fe and Cr of remain-ing metal particles had significant differences among most ferrochrome kitchen knives (P<0.05). Conclu-sion U sing GSR particle analysis function to quantitatively detect the ratio (relative amount) of Fe and Cr of remaining metal particles on ferrochrome kitchen knife, which can establish the feasible evaluation method to estimate such injury tool.

Objective To analyze and compare the short-term efficacy and daverse reaction of postoperative adjuvant radio -chemotherapy versus simple chemotherapy in advanced gastric cancer .Methods A total of 64 patients with locally advanced gastric cancer were randomly but equally divided into 2 groups ,experimental group and control group .The experimental group patients ini-tially receive 4-cycles chemotherapy with Capecitabine Tablets 1 000 mg /m2 ,bid ,d1 - 14 plus Oxaliplatin 130 mg/m2 ,d1 ,and then receive Capecitabine Tablets 825 mg /m2 ,bid ,d1 - 35 plus 3DCRT 45 Gy/25 Fx /5 w concurrent radio-chemotherapy .Control group patients receive 6-cycles Capecitabine plus oxaliplatin combined chemotherapy .Then we observed the disease progression time ,toxic reaction ,1 year survival rate and 2 year survival rate simultaneously in two groups .Results The follow-up rate were 100% .The comparative results are listed as follows (experimental group and control group) :the rates of Ⅰ /Ⅱ grades of nausea and vomiting were 87 .50% and 62 .50% respectively (P< 0 .05) ,other adverse reactions were not statistically significant ;1-year overall survival rate and recurrence-free survival rate were 90 .63% ,87 .50% respectively (P > 0 .05) and 84 .38% ,62 .50% respectively (P <0 .05) ;2-year overall survival rate and recurrence-free survival rate were respectively 84 .38% ,59 .38% (P< 0 .05) and 75 .00% , 50 .00% respectively (P< 0 .05) .Conclusion The advantages of 3DCRT combined with Capecitabine Tablets chemoradiotherapy can raise the successful rate of local control in patients with advanced gastric cancer after operation and 4 cycles chemotherapy .The toxic reactions are tolerable for patients .