The new healthcare reform project raised the thought that basic medical care service should be available to each individual,and the objective of an entire-people covered medical care assurance,which provide policy guarantee for the improvement of medical care service.To achieve the real advancement of medical care service,many endeavors are involved as follows.It is necessary to strengthen the responsibility share of all-level governments,guarantee the input for medical care adapted to local conditions and meanwhile embody medical care impartiality,strengthen the quality construction within medical care institutions,develop the role of medical care service in directing the patients triage which guides residents to get access to community medical centers,and respect individual's own choice for medical care so as to achieve the competition mechanism among medical care service designated hospitals.

BACKGROUND: There are many studies from both domestic and abroad certifying the conclusion that sport exercise can descend state anxiety even though the study outcome is diversified.OBJECTIVE: Analysis of the effects of different exercise intensities on state anxiety and physiologic index of female college students taking different exercise frequencies, in order to take exercise of adequate intensity for descending the state anxiety of female college students.DESIGN: Matched observations, selecting female college students as subjects stochastically. SETTING: Department of Military Sports, China Metrology College. PARTICIPANTS: Totally 60 students from the Management Department and Track and Field Department of Shanghai Physical Culture Institute were selected as the subjects from March 2004 to July 2004. The subjects were categorized into two groups: frequent exercise group and infrequent exercise group according to exercise frequency (3 times per week, above 30 minutes each time), and each group consisted of 30 subjects.METHODS: All subjects were tested on running stage of Swiss-made Monark power bicycle with the time length: 20 minutes, exercise intensity 30%VO2max and 75%VO2max. The exercise intensity of subjects was deter mined by the linear relationship between oxygen uptake and heart rate. The state anxiety of the students was assessed with state-trait anxiety scale which have 40 items in a survey questionnaire of two pages. The state anxiety was mainly evaluated with the state anxiety survey questionnaire of items 1-20 under stress condition, and the human emotional experiences were appraised with the trait anxiety survey questionnaire of items 21-40. Each scale was then rated from 1-4, 1 = almost nothing, 2 = a little, 3 = sometimes, 4= approximately always. The questionnaire were handed out and collected respectively in 3 time intervals (immediate, 5 minutes, 20 minutes), before and after exercise the students' heart rate and blood pres sure were tested with PoLar distance cardiotachometer and HeM-741c oMron electronic blood pressure gauge in the same time intervals before and after exercise. MAIN OUTCOME MEASURES: Comparison of the effects of different exercise intensities on the state anxiety of female college students taking exercise of different frequencies. Evaluation on their heart rate and blood pressure.RESULTS: Totally 60 survey questionnaires were handed out and 60 col lected with the collection percentage of 100%. ①Variation of state anxiety under different exercise intensity. In infrequent exercise group,as compared with those prior to the test, the state anxiety of subjects in infrequent exercise are descended remarkably (P ＜ 0.01) in 30%VO2max exercise of the 3 time intervals immediate, 5 minutes and 20 minutes, with the descent amplitude: 9.286, 24.44%; the state anxiety was descended as well in the 75%VO2max exercise of the 3 time intervals, but without remarkable descent amplitude. In frequent exercise group, the state anxiety was descended remarkably (P ＜ 0.01-0.05) in 75%VO2max exercise of the 3 time intervals with the descent amplitude: 5.575, 19.5%; the state anxiety was descended remarkably in 30%VO2max exercise for 20 minutes. ② Assessment on heart rate and blood pressure indexes. In frequent exercise group, heart rate showed temporal elevation immediately after test, and soon resumed to previous level before test;diastolic blood pressure (DBP) remained stable. Systolic blood pressure (SBP) showed temporal elevation immediately after test and SBP showed remarkable descent with the greatest degree in 75%VO2max exercise for 20 minutes. In infrequent exercise group, heart rate and SBP showed descent after elevation. SBP showed descent remarkably in 75% VO2max exercise and 30%VO2max exercise for 20 minutes, and more remarkably in 30%VO2max exercise particularly. DBP showed descent remarkably in aerobic exercise 5 minutes or 20 minutes.CONCLUSION: 75%VO2max aerobic exercise can descend noticeably the state anxiety of female college students in frequent exercise group , and 30%VO2max can eff

The article reviewed the function of general practitioners in Beijing Jiaotong University Health Service Center, which was transitioned from sole curative service to both curative and preventive services. The article also discussed the role as gatekeeper for health system played by practitioners in university health service centers.

Objective To observe the dynamic changes of the numbers of CD34 positive microvessel and RCA-1 positive cell and neutrophil in perihematoma tissue after intracerebral hemorrhage (ICH) in rats at different time points and the impact of thrombin-specific inhibitor hirudin on the above different indicators and to investigate the protective mechanisms of hirudin for brain injury following ICH. Methods An experimental model was made with autologous whole blood injecting into the rat brain basal ganglia by using the stereotactic method. The rats were randomly assigned to sham operation, ICH, hirudin intervention, and normal saline groups.CD34 and RCA-1 immunobistochemistry stainings and conventional HE staining were used to observe CD34-positive microvessel, RCA-1 positive cell and neutrophil.Results The numbers of CD34-positive microvessel began to decrease at 12 hours after ICH, it decreased to the lowest at 72 hours, and it gradually returned to normal levels at day 7. The RCA-1 positive cells could be observed at 6 hours after ICH. It reached the peak at 48 hours. A small amount could persist for two weeks. Neutrophil could be observed at 12 hours after ICH. It reached the peak at 48 hours and disappeared at week 2. The administration of hirudin significantly reduced the numbers of RCA-1 positive cell and neutrophil in the early stage of ICH (5 min). At the same time, it significantly inhibited the decreased numbers of CD34-positive microvessel (all P ＜0. 01). The administration of hirudin during the edema formation also significantly reduced the numbers of RCA-1 positive cell and neutrophil (all P＜ 0.05), however, it could not significantly increase the numbers of CD34-positive microvessel. Conclusions Thrombinmediated inflammatory response has involved in the process of brain injury after ICH, and early administration of hirudin may significantly relieve perihematoma tissue injury.

Objective To investigate antimicrobial susceptibility of Klebsiella pneumoniae (K .pneumoniae )to aminoglycosides and detection of 16S rRNA methylase genes in K .pneumoniae .Methods Ninety-six non-repetitive clinical K .pneumoniae isolates were collected from Xiangya hospital of Central South University from January to Ju-ly 2009,minimal inhibitory concentrations (MICs)of gentamycin,amikacin and tobramycin were determined by agar dilution method ;genotype of 16S rRNA methylase genes (armA,rmtA,rmtB ,rmtC,rmtD ,npmA)were detec-ted by polymerase chain reaction(PCR).Results MIC50 of amikacin,gentamycin and tobramycin was 256μg/mL, 512μg/mL and 512μg/mL respectively;and MIC90 were all>512μg/mL;antimicrobial resistance rate was 21 .88%, 63.54%,and 41 ,67% respectively.68 isolates (70.83%)were resistant to at least one kind of antimicrobial agent, 21 isolates(21 .88%)were resistant to three kinds of antimicrobial agents.22 isolates(22.92%)carried armA,but rmtA,rmtB ,rmtC,rmtD and npmA were not detected;of 22 isolates harboring armA 16S rRNA methylase genes, 17(77.27%)were highly resistant to gentamicin,amikacin and tobramycin,the homology of armA positive isolate and armA (FJ410928.1 )was 100%.Conclusion armA 16S rRNA methylase gene harbored in K .pneumoniae plays an important role in aminoglycoside resistance.

Objective To compare difference of nt and aa of H gene between the measles virus strains(MVW) recently circulating and used vacci ne strain (Chang-47) in Liaoning province of China, and to study biological an d immunological characteristic and genotype of MVW and Chang-47. Method s The analysis of 1～1 800 nt and 1～600 aa abou t H gene of MVW and Chang- 47 vaccine strain was performed. Neutralization test was performed against MVW and Chang-47 vaccine strain in the sera of measles cases and children vaccinated after 30 days. Results The genoty pe of the MVW was different from genotypes previously described in other c ountries and vaccine strain (Chang-47) used in Liaoning province. It also showe d that 18～28 aa were distinct from aa of other genotype, but most of the importa nt biological and immunological sites were not changing except the site at aa 24 0 .Neutralization antibody titer GMT against MVW and Chang-47 strains were no sig n ificantly different in the sera of measles cases and the children vaccinated af te r 30 days, but antibody titers GMT of the measles cases sera against MVW or Cha ng-47 strain were much higher than children vaccinated. Conclusions There was nt variation in the MVW. Most of biological and immunological a a sites of the MVW and Chang -47 strains were the same and were not changing. C hang -47 vaccine may also protect from infection of the MVW.

Objective To evaluate the effects of recombinant human hematopoietic synergistic factor (rhHSF) on rapid mobilization of the peripheral blood stem cells. Methods 8 monkeys were divided into rhG-CSF 10?g/(kg?day) and rhG-CSF 10?g/(kg?day) + rhHSF 500?g/(kg?day) group, with 4 monkeys in each group. All of them received rhG-CSF subcutaneously once daily for 4 days, and in the latter group, the animals received rhHSF subcutaneously in single dose on the fifth day. Results The highest counts of CD34~+ cell, CFU-GM and neutrophils in animals receiving rhHSF only were 23, 1.8 and 3.3 times of baseline value at 180, 45 and 60 min, while those in animals receiving rhG-CSF and rhHSF were 41.3, 8.3 and 4.9 times of base line value. Conclusion rhHSF can rapidly mobilize hematopoietic stem cells and neutrophils into peripheral blood, and it is found to synergize with G-CSF to augment stem cell mobilization, suggesting a potent clinical utility of rhHSF in peripheral blood stem cells transplantation.

Objective To explore the reverse effect and mechanism of progesterone(Prog) on a human tongue cancer drug-resistant cell line Tca8113/BLM in vitro. Methods The reverse effect of Prog on Tca8113/BLM was determined by MTT. The effects of Prog on the intracellular ADM congregation, cell cycle distribution and the expression of P-gp in the cell membrane were detected by flow cytometry (FCM). Results The value of IC50 of Tca8113 and Tca8113/BLM cells without Prog was 9 56 and 120 1,respectively. After treated with Prog(2umol/L),The value of IC50 of Tca8113 and Tca8113/BLM cells was 9 56 and 12 1,respectively. The potent fold of BLM in Tca8113/BLM cells was 9 92, the concentration of intracellular ADM in Tca8113/BLM cells significantly increased(P

The cultivation of college students' innovative ability should be strengthened in order to keep up with the development of our society and improve the students' quality to adapt themselves to their future career.In order to cultivate college students' innovative ability,on the one hand,we should advance quality education,enhance teaching staff training and improve teaching methods to provide helpful external factors;on the other hand,we also should equip students with solid basic knowledge,enhance their awareness of innovation,change their study concept and improve their practicing ability to provide beneficial internal causes.