A total of 62 disk-shaped specimens in the size of 30 mm (diameter) ×2 mm (layer thickness) were made with auto-polymerized acrylic resin (2 specimens for infrared spectrum comparison and 60 specimens for tests of water sorption and solubility). Duration of Ar plasma application was 2 minutes and plasma power applied was 60 W. Storage intervals were 1 day, 1 week, 1 month, 6 months, and 12 months, separately. A control group and a corresponding experimental group were set for each storage interval, with 6 specimens per group. All specimens were weighted 3 times, i.e., initial weight; weigh after absorption of distilled water, and final weight after desiccation. Percentages of water absorption and solubility of auto-polymefized acrylic resin were calculated. Results revealed that after Ar plasma treatment, C-H structure of auto-polymerized acrylic resin was reduced; following 1-month storage interval, water absorption percentage was significantly lower in the experimental group than in the control group; following 1-day, 1-week, and 1-month storage interval, the experimental group exhibited significantly lower solubility of auto-polymerized acrylic resin, compared to the control group (P<0.05). These results indicated that within 1-month storage, Ar plasma treatment can decrease water sorption and solubility of auto-polymerized acrylic resin.

AIM: To investigate the effect of Dendranthema morifolium (Ramat) Tzvel (DM) on isolated rat heart and ventricular myocytes during ischemia/anoxia and reperfusion/reoxygenation.METHODS: The Langendorff perfused rat hearts were used to measure intraventricular pressure and coronary flow. The cell contraction and intracellular calcium transient in enzymatically isolated ventricular myocytes were determined. RESULTS: (1) DM (0.5 g/L) significantly attenuated the inhibitory effects induced by ischemia/reperfusion on left ventricular developed pressure (LVDP), ?dp/dt max, coronary flow and LVDP?HR, meanwhile increased the content of SOD and decreased the content of MDA in the myocardium; (2) DM (0.5 g/L) attenuated the inhibitory effects of anoxia and reoxygenation on [Ca 2+]i transient and cell contraction in isolated ventricular myocytes. CONCLUSION: DM attenuated the effects on contractility and intracellular calcium induced by ischemia/anoxia and reperfusion/reoxygenation in the isolated rat heart and the ventricular myocytes. The mechanism might be related to increase in SOD activity and maintaining [Ca 2+]i homeostasis.

Objective To evaluate the value of real-time ultrasonic elastography in the differential diagnosis of rectal tumors.Methods One hundred patients (30 cases of adenoma and 70 cases of adenocarcinoma) with rectal tumors proved by pathology and 70 normal subjects referred to the First Affiliated Hospital of China Medical University from April 2012 to October 2013 were included in this prospective cohort. All patients underwent real-time ultrasonic elastography. The tumour tissue and reference tissue were chosen for strain ratio measurements. Only tumor tissue was selected for the tumor sample area. Tissue recognized as normal rectal wall and perirectal tissue was selected as the reference sample area. At the same time the elasticity score were calculated. Receiver operating characteristic (ROC) curves of the elasticity score and strain ratio method were used to find the cut-off point of adenoma and adenocarcinoma. The corresponding sensitivity, accuracy and specifi city were calculated. One-way ANOVA was used to comparestrain ratio value among healthy control group, retal adenoma group and rectal adenocarcinoma group and among patients with different preoperative stages of adenocarcinoma group. LSD-t test was used to compare strain ratio value between two groups.Results ROC curve showed that the best cut-off value of elasticity score in diagnosis of rectal tumor was 3, the sensitivity, specificity and accuracy were 85.1%, 73.1% and 82.0%, and the area under ROC curve was 0.780. The best cut-off value of strain ratio in diagnosis of rectal tumour was 2.34, the sensitivity, specifi city and accuracy in diagnosis were 91.4%, 83.3% and 89.0%, and the area under ROC curve was 0.945. Strain ratio of healthy control group, rectal adenoma group and rectal adenocarcinoma group was 0.74±0.44, 1.75±0.58 and 7.48±6.80. There was signifi cantly statistical difference among three groups in strain ratio. Compared with the strain ratio of healthy control group and rectal adenoma group, that of rectal adenocarcinoma group was higher (t=-8.26, P=0.000; t=-6.98,P=0.000). Compared with the strain ratio of healthy control group, that of rectal adenoma group was higher (t=-8.53,P=0.000). Strain ratio of patients with preoperative pathological stages T1, T2, T3 and T4 rectal carcinoma was 4.91±3.60, 7.07±7.23, 8.64±2.62 and 8.58±9.95 and there was no significantly statistical difference among patients with different preoperative stages of adenocarcinoma group (F=0.86,P=0.47).Conclusion The real-time ultrasonic elastography is a promising modalityfor the differential diagnosis of rectal tumors.

Objective To investigate the expression levels and the roles of IL-17 and IL-23 in children with Mycoplasma pneumoniae (MP) pneumonia. Methods One hundred and three children with pneumonia admitted to pediatric department from February to May in 2012 were divided into MP pneumonia group and non-MP pneumonia group according to the results of MP antibody tests. Meanwhile, 42 healthy children were chosen as normal controls. Serum levels of IL-17, IL-23 and MP antibodies were measured in all children. Immunoglobulin, C reactive protein, total white blood cell count and granulocyte count were detected in children with pneumonia. Results The serum levels of IL-17 and IL-23 were signiifcantly different among three groups (P<0.05). The children in MP pneumonia group had higher levels of IL-17 and IL-23 than those in non-MP pneumonia group (P<0.05). Compared with the control group, levels of IL-17 and IL-23 were higher in two pneumonia groups (P<0.05). There was no signiifcant difference in levels of IL-17 and IL-23 between pneumonia patients with normal and with abnormal levels of immunoglobulin (P>0.05), while IL-17 and IL-23 levels were both positively correlated with granulocyte count (P<0.05).Conclusion IL-17 and IL-23 may be involved in the immune response of MP pneumonia and may contribute to the clearance of pathogens.

Objective To evaluate the therapeutic effects of rAAV2-SERCA2a gene targeted to myocardium by the ultrasound mediated microbubbles in rats with myocardial infarction(MI) using speckle tracking echocardiography (STE). Methods Thirty-five male SD rats were divided into Sham group, MI group and gene therapy group. rAAV2-SERCA2a was attached to the surface of SonoVue, sulphur hexafluoride-filled microbubbles. Therapy group animals were infused with those mixed fluids through tail vein. The other two groups were infused with SonoVue microbubbles. These bubbles were infused with simultaneous echocardiography. Four weeks after ultrasound microbubbles destructed,Circumferential strain rate(SRcir), radial strain rate(SRrad) and left ventricular torsion (LVtor) of regional myocardium were measured in short-axis view using speckle tracking imaging software for each animal. Results Compared with the M1 group,there were significant increase in SRcir-S,SRcir-E, SRrad-S,SRrad-E and LVtor in rats of gene therapy group( P ＜0. 01 ). There was no significant difference between gene therapy group and sham group. Conclusions rAAV2-SERCA2a gene targeted to myocardium by the ultrasound mediated microbubbles could enhance cardiac function of rats with MI.

Objective To observe the efficacy of montmorillonite combined with oxygen in the treatment of neonatal diaper dermatitis, and to provide the basis for clinical treatment. Methods Collected 100 cases of neonatal diaper dermatitis from January 2014 to January 2017 in our hospital. The patients were divided into observation group and control group according to the order of admission. After treatment, the observation group was given montmorillonite combined with oxygen therapy, the control group was given only oxygen treatment, compared the treatment effect of the two groups of patients. Results After treatment, the total effective rate of the observation group was 100％, the total effective rate of control group was 80％, there was significant difference between the two groups in the total effective rate of clinical treatment (P<0.05).The cure time was (116.20 ± 25.79) h in the observation group and (145.72 ± 24.88) h in the control group. The difference of the cure time between the two groups was statistically significant (P<0.05). In the observation group, the erythema was found in 42 children after treatment, and the area of erosion was obviously reduced before treatment, and the surface of the lesion was dry. After treatment for 5 days, 36 cases of mild to moderate diaper dermatitis were cured. After 7 days of treatment, children are cured. In the control group, 19 cases of mild diaper dermatitis were treated with erythema dermatitis . After treatment for 5 days, the area of hips in the 22 cases was smaller than that before treatment, and the lesion was dry. After 7 days, There was no significant improvement in erosion and or exudation. Conclusion Montmorillonite powder combined with oxygen treatment of neonatal diaper dermatitis is a significant effect, is the ideal treatment for neonatal diaper dermatitis.

Objective To explore the relationship between childhood asthma and pregnant and neonatal risk factors, thus provide evidence for early prevention of childhood asthma. Methods 162 children diagnosed asthma and 213 healthy children in pediatric outpatient and the inpatient services of our hospital who was born and living in Baotou city were retrospectively analyzed. The pregnancy related factors (parity, fever during pregnancy, pregnancy-induced hypertension syndrome, gestational diabetes mellitus, history of overdose in early-pregnancy) and the neonatal period related factors (surface defects, asphyxia, rough placenta, birth weight, number of fetus during this pregnancy, gestational age, premature birth, cesarean section) were investigated. The sex and age showed no signiifcance between childhood asthma and control group. Results Eight pregnant and neonatal factors (fever during pregnancy, pregnancy-induced hypertension syndrome, history of overdose in early-pregnancy, surface defects, asphyxia, rough placenta, birth weight, premature birth, cesarean section) showed signiifcant difference between the two groups (P<0.05). Multifactor regression analysis found fever during pregnancy (OR=9.43, 95%CI:3.08~28.82), rough placenta (OR=2.15, 95%CI:1.29~3.59), premature birth (OR=5.16, 95%CI:1.53~17.39) and cesarean section (OR=4.05, 95%CI:2.40~6.86)are independent risk factors for childhood asthma. Conclusions Fever during pregnancy, abnormal placenta;premature birth and cesarean section are likely risk factors of childhood asthma.

OBJECTIVE:To ensure the stability of electronic data capture(EDC)system in drug clinical trials and to improve the quality of drug clinical trials. METHODS:The quality control system for EDC system was established and introduced from the formulation of quality control process,establishment of data standard,trial project management,daily management,trial project design,system operation,system function,etc. RESULTS & CONCLUSIONS:Data standard have been achieved through estab-lishing EDC quality control system by our hospital based on attributable,legible,contemporaneous,original and accurate principle. The management of trial project and daily management are conducted through data registration,staff training,the formulation of da-ta management plan,fault emergency treatment,database backup;multiple verification of support data,data lock and export,trial report autogeneration and other functions have been realized by formulating related standard operation instruction,program file,op-eration manual and quality record. Those aspects improve facticity,accuracy and integrality of data in clinical trials,and lay a foun-dation for further data mining.

AIM: To study the effect of salvia miltiorrhiza (SM) on cell contraction and intracellular calcium of enzymatically isolated rat ventricular myocytes during normoxia and anoxia/reoxygenation. METHODS: Contraction and intracellular calcium were determined with video tracking system and spectrofluorometric method, and the chemical anoxic method was employed. RESULTS: The ?d L /d t max , dL of cell contraction and the amplitude of [Ca 2+ ] i in the cardiomyocytes following SM treatment were decreased in a dose-dependent manner. During anoxia, the ?d L /d t max , dL and amplitude of [Ca 2+ ] i were decreased, while the diastolic Ca 2+ level was elevated compared with control group. All the contractile parameters and the diastolic Ca 2+ level were back toward pretreatment values during reoxygenation, but could not return to control level. After the treatment with SM (3 g/L), ?d L /d t max and dL of cell contraction and the amplitude of [Ca 2+ ] i were higher and the diastolic Ca 2+ level was lower than those in anoxia/reoxygenation group. CONCLUSION: SM antagonized effects of anoxia and reoxygenation on cell contraction and intracellular calcium in isolated ventricular myocytes.

Objective To investigate the role of outer membrane protein in clinical isolated car-bapenem resistance Acinetobacter baumannii. Methods Carbapenem resistance and sensitive strains were collected from the same patient. After MIST and REP-PCR analysis, carbapenemases were detected by isoe-lectric focusing. Different expressed membrane proteins were identified by two-dimension electrophoresis and mass spectrometry analysis. We also used efflux pump inhibitor PAβN(Phe-Arg-β-naphthylamide) to con-firm the phenotype. Results Carbapenem resistance and sensitive strains were attributed to the same pat-tern. At positions of P17.6 and P19.0, two β-lactamases were expressed in two investigated strains, no cabapenemases were detected. Six differential expressed membrane proteins were identified, a 34 × 10~3 membrane protein that was confirmed by efflux pump inhibitor PAβN experiment (imiponem MIC decreased from far above 32 μg/ml to 8μ/ml) and OprD and CarO. Conclusion Up-regulation of exported protein accompanied with down-regulation of OprD and CarO other than carbaponemases are responsible for carbap-enem resistance in A. baumannii.