1.Application of HPLC fingerprint to optimization of extraction process of Herba Artemisiae scopariae
Chinese Traditional Patent Medicine 1992;0(06):-
AIM:To optimize the extraction process of Herba Artemisiae scopariae in Yinchenhao Decoction.METHODS:On the basis of HPLC fingerprint of Herba Artemisiae scopariae uniform design was adopted to the extraction process,with four factors,including solvent concentration,solvent dosage,extraction cyde and extraction times in nine levels.Finally,the best extraction conditions were chosen according to the similarity of HPLC fingerprint and the extraction efficiency.RESULTS:The optimal extract procedure was refluxing for 90 mins with 25 times solvent of(11.14%) ethanol for three times.CONCLUSION:HPLC fingerprint is useful for the optimization of extraction process of Herb Artemisiae scopariae.
2.The Application of Humanistic Service Managing Mode in the Management of NICU
Chinese Medical Ethics 1994;0(05):-
Objective: To explore the application and clinical effects of humanistic service management in the management of NICU.Method: Humanistic service,management and education are conducted among neonate patients,their family members,nursing staff,and nursing students in NICU,and the outcome clinical effects are investigated.Result: The application of humanistic service dramatically increases the positivity,activeness,creativity,and cohesiveness of nursing staff,improves their overall quality,helps to establish a harmonious relationship between nurses,nursing students,and neonate patients' family members,enhances the satisfaction degree of neonate patients' family members and the nursing standard in NICU,and promotes the general improvement of hospital nursing service.Conclusion: The application of humanistic service in NICU initiates nursing service,improves hospital nursing standard,promotes the general development of NICU nursing,thus deserves to be further extended and applied.
3.Study of the individualized health management of senile mental disorders
Chinese Journal of Geriatrics 2014;33(8):884-886
Objective To evaluate the effect of the individualized health management on senile mental disorders.Methods 421 patients with senile mental disorders were randomized into two groups:the intervention group (n =213) received routine antipsychotic drugs treatment,health education and individual health management,and the control group(n=208) received only population health education and routine drug antipsychiatric treatment.Results The Brief Psychiatric Rating Scale (BPRS) scores in two group were significantly higher after treatment than before treatment (all P<0.01).The BPRS score in each period after treatment were significantly higher in intervention group than in control group (all P<0.01).The mini mental state examination (MMSE) score in two treatment groups were significantly higher after treatment than before treatment (all P<0.01).The MMSE score in each period after treatment were significantly higher in intervention group than in control group (all P<0.05).Conclusions The individualized health management in senile mental disorders is not only feasible,but also effective.
4.Multilocus sequence typing and genetic diversity ofCandida albicans in patients with vulvovaginal candidiasis
Zhiheng WANG ; Chunmei YING ; Hu ZHAO
Chinese Journal of Infection and Chemotherapy 2016;16(3):330-335
Objective To investigate the molecular epidemiology of 114C. albicans strains isolated from the vaginal discharge of female patients treated in three obstetrics and gynecology hospitals in Shanghai by analyzing the relationship between the main genotypes and resistance proifle, and the relationship between genetic diversity and cluster ofC. albicans.Methods A total of 114 strains ofC. albicans were collected from the Obstetrics & Gynecology Hospital of Fudan University, Shanghai First Maternity and Infant Hospital Corporation and the International Peace Maternity & Child Health Hospital of China welfare institute. Phylogenetic analysis of strains were carried out by eBURST.C. albicans strains were also analyzed by multilocus sequence typing (MLST). The susceptibility of theC. albicans strains was tested by ATB FUNGUS 3.Results A total of 47 diploid strain types (DSTs) were identiifed from the 114 strains, 30 of which were known types. DST 79 and DST 435 were the main types. Of the 114C. albicans strains, 96.5% were susceptible to lfucytosine, 100% to amphotericin B, 85.1% to lfuconazole, 55.2% to itraconazole and 84.3% to voriconazole.Conclusions The pathogenicC. albicans strains isolated from different obstetrics and gynecology hospitals in Shanghai were originated from multiple clones, the main type of which was DST 79 and DST 435 with certain degree of antifungal resistance. MLST typing suggests that genetic diversity is present in theC. albicans strains isolated in Shanghai area. The clustering analysis ofC. albicans strains is consistent with its genotypes.
5.Clinical analysis of otogenic extracranial and intracranial complications.
Chunmei HU ; Gang HE ; Chuanyu LIANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2015;29(10):903-905
OBJECTIVE:
To study the clinical feature and treatment of extracranial and intracranial complications caused by otitis media.
METHOD:
Three hundred and twenty patients of acute and chronic otitis media were admitted to our department between 2005 and 2014. Among them, 34 patients were diagnosed with extracranial and intracranial complications. The clinical features and treatment outcome were retrospectively studied. Of the 34 patients associated with complications, 25 had a single complication,8 had two complications and 1 had three complications. Complications included labyrinthitis in 14 cases, facial paralysis in 11, postauricular subperiosteal abscess in 6, Bezold abscess in 1, thrombophlebitis of sigmoid sinus in 2, otitis meningitis in land otogenic brain abscess in 8.
RESULT:
Thirty-three patients were cured or improved and 1 patient died.
CONCLUSION
Due to the widespread use of antibiotics, the clinical manifestations of extracranial and intracranial complications of otitis media become more hidden and atypical. The surgery is the primary treatment method.
Brain Abscess
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complications
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Chronic Disease
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Facial Paralysis
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complications
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Humans
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Labyrinth Diseases
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complications
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Mastoiditis
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complications
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Meningitis
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complications
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Otitis Media
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complications
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physiopathology
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Retrospective Studies
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Treatment Outcome
6.Application of pure brilliance imaging in diagnosis of benign and malignant breast neoplasms
Xiaoqiu DONG ; Xiaohui SHAO ; Chengyi HU ; Chunmei XU
Chinese Journal of Ultrasonography 2009;18(4):329-331
Objective To evaluate the application of pure brilliance imaging(PB1) in diagnosis of benign and malignant breast neoplasms. Methods Ninty-seven breast neoplasms were respectively examined by high frequency ultrasound and PBI before operation,then correlated with pathological results. Results The accuracy of high frequency ultrasound for breast neoplasms was 73.2% with the sensitivity 77.8% ,specificity 69.2%. The detectable rate of spiculate margin, halo,calcification were much higher in PBI than those in high frequency ultrasound(P<0.05). And the accuracy,sensitivity and specificity of PBI were 86.6%, 91.1%, 82.6%. Conclusions PBI can significantly increase the detectable rate of ultrasonographie marginal features of breast neoplasms which were also helpful to the differential diagnosis.
7.An analysis of clinical characteristics, etiologies and prognosis of 218 patients with infective endocarditis
Hongmei XIE ; Bijie HU ; Chunmei ZHOU ; Qing ZHOU ; Xiaodong GAO
Chinese Journal of Internal Medicine 2014;53(5):363-367
Objective To describe the profile of patients with infective endocarditis (IE) and assess prognostic factors of IE.Methods Clinical and etiology data of 218 patients with IE were collected retrospectively from January 2011 to January 2013.The distribution and antimicrobial susceptibilities of pathogens causing IE were evaluated.Prognostic factors associated with IE were determined by univariate and multivariate regression analysis.Results There were 148 men and 70 women with age of (46.0 ± 14.6)years.Ninety-five (43.6%) of them had heart diseases,including 72 cases (33.0%) of congenital heart disease and 23 cases (10.6%) of chronic rheumatic heart disease.Vegetations were detected by echocardiography in 171 (78.4%) patients.Microorganisms causing IE were identified in 84 cases (38.5%) cases.Streptococcus viridans was the dominant pathogen,accoumed for 63.1% of all the pathogens,followed by Staphylococcus (13.1%) and Enterococcus (4.8%).Totally 7/11 Streptococcus viridans was susceptible to penicillin,while 100% susceptible to the third and fourth generation cephalosporins,vancomycin and linezolid.One hundred and eighty cases underwent operations.The in-hospital mortality rate of IE was 3.2%.In univariate regression,health care-associated infection,prosthetic valve,anemia and chest symptoms (distress or pain) were related to the increased risk of mortality in patients with IE,while surgery appeared to be a protective factor.In the logistic regression model,the variables significantly associated with IE prognosis were health care-associated infection (OR =17.03,95% CI 1.76-164.75,P =0.014) and anemia (Hb < 90 g/L) (OR =13.47,95% CI 2.46-73.60,P =0.003) and surgery treatment (OR =0.17,95% CI 0.03-0.97,P =0.047).Conclusions Although Streptococcus viridans is the most common pathogen causing IE,the pathogens of IE become versatile.The antibacterial activity of penicillin against Streptococcus viridans is low.Health care-associated infection and anemia are risk factors of IE prognosis,while surgery treatment is a protective factor of severe IE.
8.Research on the pediatric intestinal obstruction and serous endotoxemia in 43 cases
Lan HU ; Song QING ; Chuan YANG ; Yingcun LI ; Chunmei JING
Chongqing Medicine 2015;(5):631-632,635
Objective serous endotoxin(ET) were measured to determine the relationship between serous endotoxin and differ‐ent type of intestinal obstruction in children .Methods 25 inguinal hernia patients and 43 intestinal obstruction patients were classi‐fied into control group(n=25) ,incomplete intestinal obstruction (n=20) and complete intestinal obstruction group without necrosis of intestine (n=23) according to degree of obstruction .Serous endotoxin were measured in different time (the day of admission ,one day after admission or ,preoperation and one day postoperation ) ,and t test is used to determine the difference between them . Results ET in different group was(6 .53 ± 14 .96) ,(4 .40 ± 1 .15) ,(4 .20 ± 1 .20) ,(4 .09 ± 1 .31) ,(3 .70 ± 1 .46)EU/mL ,respec‐tively .There were no difference between each group .Conclusion ET examination suggests there is no difference between different type of intestinal obstruction in children and endotoxemia is not found in intestinal obstruction according to the results .
9.Determination of Phospholipid Content in 5-FUR Prodrug Liposome
Li ZHU ; Lijun PAN ; Chunmei HU ; Junyi ZHAO ; Chi WANG
China Pharmacy 2001;0(07):-
OBJECTIVE: To determine the concentration of phospholipid in 5-FUR prodrug liposome. METHODS: The sample was fragmented by ethanol and extracted by chloroform. The concentration of phospholipid in 5-FUR prodrug liposome was determined by ammonium ferrothiocyarate spectrophometry at a wavelength 488 nm. RESULTS: The linear range of phospholipid was 0.01~0.10 mg?mL-1(r=0.999 6, n=3) with an average recovery rate of 99.66% (RSD=3.17%). CONCLUSION: This method is simple, convenient and sensitive, and it can be used for the quality control of 5-FUR prodrug liposome.
10.Preparation and Stability of Tetrandrine Liposomes
Chunmei HU ; Li ZHU ; Junyi ZHAO ; Chi WANG ; Lijun PAN
China Pharmacy 2005;0(13):-
OBJECTIVE: To draw a comparison between the two preparative technologies of tetrandrine liposomes. METHODS: Tetrandrine liposomes were prepared with ammonium sulfate transmembrane gradient method and film dispersion method respectively. Leaking ratio,particle size,and content of phospholipid of liposomes were selected as the parameters to indicate the stability of liposomes,and the entrapment efficiency was taken as quality index to compare the two preparation technologies. RESULTS: Tetrandrine liposomes prepared with ammonium sulfate transmembrane gradient technology achieved high entrapment efficiency of 81.1% and good stability,while those prepared by film dispersion method had an entrapment efficiency of only 32.9% with poor stability. CONCLUSION: Ammonium sulfate transmembrane gradient method is feasible and superior to the film dispersion method in the preparation of tetrandrine liposome.