This paper introduced the concept,principles and types of bio-remediation systematically and focused on discussing the research progress of plant,microorganism and animals on remediation techniques of heavy metals contaminated soils.Suggestions were presented based on the above discussion,which could provide reference for the future studies.

Objective To investigate the clinical efficacy of triple method of heat-sensitive point moibustion and Taiji acupuncture plus massotherapy in treating facial spasm.Method Sixty patients with facial spasm were randomly allocated to treatment and control groups, 30 cases each. The treatment group received triple method of heat-sensitive point moibustion and Taiji acupuncture plus massotherapy and the control group, conventional acupuncture plus suspended moxibustion with moxa stick. After three courses of treatment, the clinical therapeutic effects on spasm (severity and frequency) were compared between the two groups. Result The total efficacy rate and the cure and marked efficacy rate for spasm (severity) were 86.7% and 43.3%, respectively, in the treatment group and 66.7% and 26.7%, respectively, in the control group; there were statistically significant differences between the two groups (P<0.01). The total efficacy rate and the cure and marked efficacy rate for spasm (frequency) were 83.3% and 46.7%, respectively, in the treatment group and 66.7% and 30.3%, respectively, in the control group; there were statistically significant differences between the two groups (P<0.01).Conclusion Triple method of heat-sensitive point moibustion and Taiji acupuncture plus massotherapy is an effective way to treat facial spasm.

ObjectiveTo explore the effect of attention distraction in nursing children with maxillofacial trauma. Method Eighty-eight children with maxillofacial trauma were divided into control group and observation group with 44 patients in each group.Patients in the observation group was cared with attention distraction while the control group by traditional method of forced bondage.The two groups were compared in terms of wound treatment time and healing time,comfort degree and compliance of children.Results The wound treatment time and healing time in the experiment group were shorter than those in the control group.The comfort degree and compliance of children in the observation group were better than those in the control group(P<0.05).Conclusion Attention distraction for nursing children with maxillofacial trauma can improve children’s comfort and treatment compliance and shorten treatment time.

Objective To investigate the clinical efficacy and safety between laparoscopic and transabdomi -nal hysterectomy ,reduce the occurrence of complications measures .Methods On 154 cases of hysterectomy indica-tions were nonrandomly divided into two groups ,one group for the observation group of 79 cases of laparoscopic opera-tion,another group was control group of 75 patients with transabdominal operation;two groups had no significant differ-ence in age and disease .The clinical effect ,postoperative complications and safety between the two groups during and after operations were observed .Results The operation time of the laparoscopic group (72.0 ±13.2) min was signifi-cantly shorter than that of the control group (98.0 ±20.7)min (t=3.396,P=0.021);The bleeding quantity of the laparoscopic group(96.0 ±75.4)mL was significantly shorter than that of the control group (159.4 ±98.0)mL(t=9.680,P=0.014);The aerofluxus time of the laparoscopic group (17.2 ±4.2) h was significantly shorter than that of the control group(28.6 ±6.1)h(t=9.760,P=0.013).The average postoperative hospital stay time was significantly shorter in laparoscopic group(5.2 ±1.9)d than those in abdominal group(9.5 ±3.2)d (t=-5.025,P=0.018). The analgesic used in the laparoscopic group (3/89)was significantly shorter than that in the control group (48/78) (t=-8.820,P=0.000).Conclusion Laparoscopic hysterectomy is safe and effective .It is worth to promote in clinical,while improving the operation skills is the key to reduce the complications .

Objective: To study laparoscopic surgery for ectopic pregnancy complicated by hemorrhagic shock clinical efficacy and nursing research. Methods: Retrospective analysis of 60 cases of ectopic pregnancy complicated by hemorrhagic shock patients, divided into:the experimental group and control group of 30 cases, control group using traditional open surgery, laparoscopic surgery treatment groupclosely observe the clinical efficacy of the two groups. Results: Laparoscopic treatment of ectopic pregnancy in patients with hemorrhagic shock complicating operative time, blood loss than the control group; laparoscopic treatment of ectopic pregnancy in patients with hemorrhagic shock complicated exhaust time, postoperative morbidity and hospital stay was significantly lower than control group. Conclusion: Ectopic pregnancy laparoscopic surgery can improve concurrency cure rate of patients with hemorrhagic shock, better retention in patients with reproductive function, improve the patient's quality of life.

S100A6 is the member of the S100 family of EF-hand calcium-binding proteins,which participates in inflammatory reaction and regulates in cell growth,differentiation,growth inhibition and apoptosis.Recently it has been reported that S100A6 plays a key role in neoplastic transformation,proliferation and invasion of tumor,it may become a potential target in the diagnosis and treatment of tumor.Here is to review the recent advances in the research on this molecule,including its structure,function mechanism,and its relationship with the gastrointestinal cancer and so on.

ObjectiveTo investigate the variation of T lymphocyte subsets and serum immunoglobulin for perioperative patients with thyroid cancer.Methods74 cases in our hospital for radical surgical resection treatment and 131I treatment after surgery were chosen as the observation group.Take the level of T lymphocyte subsets and serum immunoglobulin for statistics.And then compared with 40 healthy people.ResultsThe observation group before surgery:NK cells:( 19.1 ± 4.6) ％,T cells:(68.2 ± 5.5 ) ％,the serum immunoglobulin before surgery was ( 13.4 ±1.4μg/L).It was significantly higher than the control group [ ( 15.2 ± 3.2 ),( 65.2 ± 5.2 ),( 4.3 ± 0.5 ) ] ; CD4+(32.1 ± 2.9),CD4+/CDs+ ( 1.1 ± 0.3 ).It was significantly lower than the control group ( 38.1 ± 3.9,4.3 ± 0.5 ).The difference was statistically significant ( t =24.5,17.8,33.6,14.5,22.5,all P ＜ 0.05 ).The observation group after surgery,1 month after 131I treatment,3 months after 131I treatment,NK cells ( 17.0 ± 3.3 ),( 16.9 ± 3.0),( 16.7 ±3.4) and serum immunoglobulin (5.1 ±0.6),(6.2 ± 1.0),(6.1 ± 1.0) were significantly higher than control group,the difference was statistically significant ( t =18.4,11.6,9.2,5.4,8.1,7.3,all P ＜ 0.05 ).ConclusionThe variation of T lymphocyte subsets and serum immunoglobulin for perioperative patients with thyroid cancer was regular,and could be used as one of the indicators to determine for tumor recurrence or metastasis.

Objective To establish a set of methods of Chinese materia medica quality evaluation based on biothermodynamics.Methods Using microcalorimetry,the thermal power of Escherichia coli meta-bolism affected by Rhizoma Coptidis in different growing years was measured.According to the thermokinetic model,the relationship of the thermokinetic parameters,caloric bioactivity and chemical constituent was analyzed.Results There were obvious difference in the caloric active fingerprints of E.coli metabolism affected by Rhizoma Coptidis in different growing years,which presented significant finger character.Compared with the normal groups,the metabolism of E.coli was inhibited in different levels by Rhizoma Coptidis in different growing periods,the multiplication rate constants and heat output were decreased,and the generation time was prolonged.Especially,Rhizoma Coptidis under four years' growing period with the highest content of total alkaloids showed a significant inhibitory effect on E.coli.Conclusion There are evident correlation among the thermokinetic parameters of E.coli metabolism affected by Rhizoma Coptidis in different growing years,the caloric bioactivity,and chemical composition.The thermokinetic parameters can indirectly reflect caloric bioactivity of Rhizoma Coptidis.As the method of biothermodynamics is on line,microamount,high throughout screening,and universality.The microcalorimetry is considered to be a useful mean for Chinese materia medica quality evaluation.

Objective:To probe the possibility of using STR polymorphism on chromosome 21 to diagnose Down's syndrome.At the same time,some parents of Down's syndrome were analysed using the same motbed.To develop a rapid anti accurate method for Down's syndrome diagnosis and review its pattern.Methods:The small tandem repeats(STR)of D21S11 and D21S1414 on chromosome 21 were analysized by polymerase chain reaction(PCR).100 Down's syndrome samples(blood)and 20 parents' samples(blood)were analyzed with.denaturing polyacrylamide gel(8%)and Silver Nitrate staining.Results:(1) The normal person and two kinds of status in the genotype of a STR gene:①Two bands of same size on the gel electrophoresis were displayed to the heterozygote of two different alleles.②A band on gel electrophoresis was shown to the homozygote of two same allele But the width and thickness of the band were about two times of a single one.(2)There were three kinds of characteristic changes of the number and/or the bands thickness of allele for the Down's Syndrome:①Three different alleles,with three bands of same thickness on gel electrophoresis.②Only two of the three alleles are the same,two bands on gel electrophoresis,and one of the two bands was two times thicker than the other.③Three alleles were the same, only one band on gel electrophoresis.The width and thickness of the band are about three times than single band of normal.(3) The analysis about parents of 20 cases Down's syndrome shows that the two bands were usually derived from mother,and another derived from father in bands of the Down's syndrome on gel electrophoresis.Conclusions:(1)As genetic marker with STR sites (D21S11 and D21S1414)of chromosome 21,Down's syndrome can be diagnosed by PCR,denaturing polyacrylamide gel and Silver nitrate staining analysis.(2)Genetic analysis of the parents of Down's syndrome show that onset of Down's syndrome does not have relationship with the karyotype of the parents.Besides,the extra chromosome 21 is derived from mother usually.