Objective To compare the contents of astragaloside Ⅳ and polysaccharides in radix astragali with different growth years, providing reference for the better harvest time of radix astragali. Methods HPLC-ELSD was used to determine the content of astragaloside Ⅳ. A Hypersil-Keystone C18 column was used with the mobile phase consisting of acetonitrile-water (33∶67). The flow rate was set at 1.0 mL/min. The parameters of drift tube and gas flow rate of the detector were set at 105 ℃ and 2.7 L/min respectively. Phenol-sulfuric method was employed to determine the content of polysaccharides, and the detection wavelength was set at 490 nm. Results The linear range of astragaloside Ⅳ was within 1.25-6.28 μg. The average recovery was 97.28%, with RSD=1.42%. The linear range of polysaccharides was 10-100 μg. The average recovery was 99.02%, with RSD=0.94%. The content of astragaloside Ⅳ in radix astragali cultivated for more than 1 year was higher than the standard in Chinese Pharmacopoeia, and radix astragali cultivated for 3 years showed the highest content. The content of polysaccharides in radix astragali cultivated for more than 3 years was the highest, and radix astragali cultivated for 2 years was slightly lower. Conclusion The better harvest time for radix astragali is at least two years of growth, which is judged from the content of astragaloside Ⅳ and polysaccharides.

Objective To explore the diagnostic value of microRNA(miR)-451a,miR-3613-5p and miR-125b-5p in endometriosis(EMT).Methods A tatol of 35 patients with EMT in the Department of Gynecology,the Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine were selected as the case group from December 2021 to February 2023,30 healthy women of childbearing age were selected as the control group over the same period.The expression levels of miR-451a,miR-3613-5p and miR-125b-5p in the plasma of two groups were measured using real-time quantitative reverse transcription polymerase chain reaction,the diagnostic value of these miRNAs in EMT was evaluated by using receiver operating characteristic(ROC)curve.Results ①The expression levels of miR-451a,miR-3613-5p and miR-125b-5p in case group were higher than those in control group,the difference was statistically significant(P＜0.05).②In case group,there was no significant difference in miRNA expression levels between mild-moderate patients(stage Ⅱ-Ⅲ)and severe patients(stage Ⅳ)(P＞0.05).The expression levels of carbohydrate antigen 125(CA125)in plasma was significantly different(P＜0.05).③The diagnostic efficacy of miRNA for EMT was evaluated through ROC analysis,with a sensitivity of 85.7％and specificity of 40.0％for miR-451a,a sensitivity of 60.0％and specificity of 90.0％for miR-3613-5p,and a sensitivity of 60.0％and specificity of 76.7％for miR-125b-5p.Combined with a sensitivity of 48.6％and specificity of 96.7％.Conclusion Compared to healthy women,the expression levels of miR-451a,miR-3613-5p and miR-125b-5p in plasma of EMT are increased,which are not affected by clinical stage,and are expected to be candidate markers for the non-invasive diagnosis of EMT,and are not affected by clinical staging,making them promising candidate biomarkers for non-invasive diagnosis of EMT.