Objective To observe the effect of minocycline hydrochloride sustained-release agent on interleukin-6 (IL-6) in gingival sulcus fluid of patients with chronic periodontitis.Methods One hundred and twenty patients with chronic periodontitis were randomly divided into two groups,the control group(n =60 cases) and the treatment group(n =60 cases).The patients in the control group were treated through oral tinidazole.The patients in the treatment group were given topical application of minocycline hydrochloride sustained-release agent.The plaque index,probing pocket depth,bleeding on probing rate and IL-6 in gingival sulcus fluid were detected before and after treatment.Results The plaque index,probing pocket depth,bleeding on probing rate were decreased after treatment.There was a significant difference between two groups(P ＜ 0.05).IL-6 level was decreased after treatment.There was a significant difference between two groups (P ＜ 0.05).Conclusion Minocycline hydrochloride can improve patients with chronic periodontitis through mitigating inflammation.

Objective To evaluate the application value of bronchofibroscope in severe pulmonary infection.Methods 60-severe pulmonary infections were diagnosed.They were divided into two groups by random digits table.The control group were treated with conventional therapy (include:antibiotic therapy,eliminate sputum、nutritional support,and so on).The observation group were treated with not only conventional therapy but also bronchial lavage and antibiotic therapy by bronchoscope.Indicatrix and curative effect of clinical were analyzed by statistics-software.Results After treatments,The time of hemogram recoved (11.50 ± 4.31) days,and the time of Clinical symptom dispersal(12.31 ± 2.30)days and the time of fever(7.02 ± 2.23)days in the observation group were all shorter than those of control group [(16.11 ± 5.21) days,(20.09 ± 5.19) days,(11.04 ± 3.31) days].All comparisons were statistical significance(t =5.03,6.87,6.22,all P ＜ 0.05).The total effective rate was 88.5％ in the observation group,whcih was efficienter than that of the control group (62.5 ％) (x2 =4.36,P ＜ 0.05).Conclusion It is effectively to use a bronchial lavage and antibiotic therapy treated severe pulmonary infections by bronchoscope.

An unusual case of primary cutaneous anaplastic large cell lymphoma induced by influenza vaccination is reported.A 79-year-old man developed an erythematous nodule at the injection site on the upper left arm 2 months after vaccination against influenza A virus,which gradually grew and ulcerated.Pathological examination of the lesion revealed epidermal infiltrate of lymphocytes and numerous clumps of densely distributed lymphocytes with lightly stained cytoplasm,nuclear atypia and karyokinesis.Immunohistochemistry showed that the tumor cells stained positive for CD3 (90％),CD5 (90％),LCA (90％),CD30 (90％),CD10 (a few),Ki-67 (80％),but negative for CD20,CD56,cytokeratin 1-3,epithelial membrane antigen,synaptophysin,and anaplastic lymphoma kinase.The patient was diagnosed with primary cutaneous anaplastic large cell lymphoma at the injection site following vaccination against influenza A virus.

Objective To study the influence of excessive magnesium and/or deficient calcium intake on the bone mass of ovariectomized rats. Methods Three-month-old ovariectomized rats were fed with different types of drinking water: Interaction group was fed with deficient-Ca and excessive-Mg water: Ca 5 mg/L, Mg 60 mg/L; Excessive-Mg group was Ca 60 mg/L, Mg 60 mg/L; Deficient-Ca group was Ca 5 mg/L, Mg 30 mg/L; Normal group was Ca 60 mg/L, Mg 30 mg/L. After 3 months of treatment, the left femur was measured with the length and weight, then the content of Ca and Mg were determined, right femur was X-rayed, and right tibia was taken for the pathological examination. Results Calcium deficiency in water caused significant changes in all of the bone mass indexes, magnesium excess in water showed a tendency of Ca decreasing and Mg increasing in the bone. It was easily distinguish the difference in bone mineral and bone microstructure by X-ray and pathological examination: control group was the best, then was Mg excess group, Ca deficiency group was inferior, and the interaction group was the worst. Conclusion Calcium deficiency is one of the major reasons of osteoporosis, while the excessive intake of magnesium may not only disturb the bone metabolism but also aggravate the adverse effect of Ca deficiency on bone mineral and bone health.

One hundred and fifty-seven cases of Graves' disease were divided into three groups according to their clinical features and thyroid functions: Group A, untreated; group B, treated but uncontrolled or relapsed; group C, treated and remitted. The positive rates of TSH receptor antibody (TRAb), anti-tnyroglobulin antibody (TgAb), anti-microsomal antibody (TmAb), anti-DNA antibody and anti-nuclear antibody (ANA) were 91.2, 79.4, 79.4, 70.6 and 23.5% respectively in group A. The positive rate of TRAb and TBII were significantly different between group C and group A or B (both P

Objective To investigate fracture patients' perception about pain and analgesic.Methods In-depth interview for 30 min was done for 20 fracture patients within 12 h after their admission.Data were analyzed based on Colaizzi phenomenological research method.Results Fracture patients underestimated the influence that pain had dosne to them.When they were suffering from pain,most of them chose to endure until they couldn't endure anymore.For analgesics,most of them chose to avoid from using them unless they couldn't endure anymore.Conclusions Pain management education should be done to help patients correct their bias about pain,know more about analgesics according to their personal conditions,and help them self-report their pain and manage their pain timely and properly.

Objective To establish a statistical model that can quantitatively analyze the dosimetric sparing of the bladder based on individual patient’ s anatomy in the static intensity-modulated radiotherapy (IMRT) plans for rectal cancer.Methods Static IMRT plans (7 AP fields) for 100 rectal cancer patients were used to train the model from 2012 to 2013.The anatomical features were quantitatively analyzed by the sizes of overlap regions of bladder-planning target volume (PTV) and bladder-PTV+0.5(0.5 cm margin around the PTV) .The mathematic relationship between anatomical features and dosimetric sparing of the bladder was evaluated after the bladder sparing dose was analyzed using dose-volume histogram.The established model was verified in the IMRT plans for additional 20 rectal cancer patients.Results Bladder V50 was linearly correlated with the ratio of bladder-PTV overlap size to bladder volume ( denoted as x%) , with an equation of V50=0.89x-0.99.Bladder V40 showed an approximately linear correlation with the ratio of bladder-PTV+0.5 overlap size to bladder volume (denoted as y%).The mean dose depended on both x%and y%.For the additional 20 plans, the absolute deviation between predicted and actual values for V50 and V40 were (-3.13%-3.78%) and (-5.30%-5.66%) , respectively, and the relative deviation for the mean dose was (-3.94%-3.76%) .Conclusions The model obtained in this work provides an effective method for quantitatively estimating the bladder sparing dose in IMRT plans for rectal cancer.

Objective To assess whether mTOR was activated in peripheral T lymphocytes of acute coronary syndrome (ACS)patients and to investigate the relationship among mTOR,regulatory T cells and cytokins. Methods Patients with acute coronary syndrome (n=32) were selected in ACS group. Meanwhile, patients who complainted of chest pain but were proven to be normal in ECG and in coronary arteriography, were excluded as ACS patients but were diagnosed as Chest Pain Syndrome (CPS) and selected in CPS group (n=28). The expression levels of mTOR, phospho-p70S6KT389 (indicative of mTORC1 activity) and phospho-AKTS473(indicative of mTORC2 activity) were investigated in T cells, which were isolated from peripheral blood of patients in ACS group or CPS group, using Western blot. The proportion of CD4+CD25+Foxp3+Tregs over CD4+cells were evaluated by FACS. And T cell subset related cytokines such as IFN-γ, IL-4, IL-17 and TGF-β1 were exam?ined by ELISA. Then we investigated the relationship among mTOR,regulatory T cells and cytokins by Spearman analysis. Results mTOR and p-p70S6KT389 expression were significantly enhanced in ACS group as compared with those in patients from CPS group (P<0.01). Higher levels of IFN-γ, IL-17 but lower level of TGF-β1 cytokines as well as decreased propor?tion of Tregs were observed in ACS group than those in CPS group (P<0.05). There was no significant difference in the level of IL-4 and p-AKTS473 between the two groups (P>0.05). By correlation analysis, p-p70S6KT389 expression level positively correlated with IFN-γ, IL-17(rs=0.91,092,P<0.01)and negatively correlated with Tregs and TGF-β1(rs=-0.85,-0.80, re?spectively, P<0.01). Conclusion mTORC1 pathway was activated in peripheral T lymphocytes of ACS patients, and Tregs insufficiency and cytokins imbalance both contribute to the activation of mTORC1 pathway and pathological process of ACS.

OBJECTIVE:To investigate the effect of salesclerk factors on the consumers'purchasing behavior in drugstores in Beijing citizens,and provide reference for better satisfaction of consumers'requirements. METHODS:Clustering random sampling method was launched among the drugstore consumers in 16 districts of Beijing,the results were statistically analyzed,and then the suggestions were provided. RESULTS:Totally 180 valid questionnaires were collected from the 208 questionnaires with effective re-covery rate of 86.5%. 70.6% consumers preferred the salesclerk to ask actively when they entered a drugstore;moderate depen-dence on the salesclerk was found mainly in the aspect ofmedicine selection,medication methods guidingandhelping disease judgment;moderate trust to the salesclerk was found,andthe poor professional knowledge,poor service attitudeandpoor professional ethicswere the main reasons for the distrust;the professional moralandknowledgeof salesclerks were the most-ly expected qualities;the licensed pharmacist in the drugstore was not recognized by 66.1% consumers. CONCLUSIONS:Drug-store should understand the consumer's expectations and requirements to its salesclerk and improve accordingly in the respect of strengthening the train of professional quality,enhancing the ability of pharmaceutical care,increasing the propaganda of licensed pharmacist,conducting medication consultation actively,exploring the diversity of consumer's requirements,and meeting the con-sumer's requirements more extensively.

Objective To make a passive personal sampler for the determination of the styrene in the air. Methods To make a passive personal sampler for the determination of the styrene in the air. Results Under the condition of wind velocity of 100-600 cm/s, relative humidity of 40%-80% and temperature of 10-40 ℃, the sampling flux of the passive sampler was 61.8 ml/min, the capacity was 5.95 mg, the shortest sampling time was 30 min, the self life was at least 30 days, the storage stability was at least 14 days, the RSDs were 8.07%-9.37%. Conclusion This new kind of passive personal sampler can be used as a new device for the determination of the styrene in the air.