Objective To explore the mechanism of imperfect recovery of the renal function after release of chronic ureteral obstruction. Methods Adult New Zealand rabbits(n=36) were randomly divided into normal control group(n=3),obstructive control group(n=3) and experimental group(n=30).The latter two groups were subjected to chronic unilateral ureteral obstruction.Two weeks later,3 rabbits were sacrificed and the others underwent ureterostomy,which were sacrificed separately following 12 hours,24 hours,3 days,7 days,14 days.The renal papilla and cortex were examined by scanning electron microscopy. Results Shortly after the release of ureteral obstruction,massive loss of brush border and ballooning of epithelial cells were noted in proximal tubules.Distal tubules and collecting tubules also showed severe ballooning of epithelial cells and normal struction were not seen.Two weeks later,many tubular components became patent with normal epithelial lining.Some of them,however,were deteriorated further in spite of the release of obstruction.Thus there was a marked tubular heterogeneity in recovery.Conclusion Further deterioration of some tubules might be one of the important pathologic basis for imperfect recovery of renal function after the release of chronic ureteral obstruction.

Objective To explore the effects of ureteropelvic junction(UPJ) obstruction on contralateral kidney.Methods Twenty-four adult New Zealand rabbits were divided randomly into 3 groups,ie,complete obstruction group(group 1,n=8),partial obstruction group(group 2,n=8) and shamoperation group(group 3,n=8).In groups 1 and 2, the right ureter was ligated completely or partially.In group 3,the right ureter was only exposed but not ligated.The animals were killed 4 weeks after operation,and the contralateral kidneys were resected.Before killing,anterior urography showed that right hydronephrosis occurred in all the rabbits in groups 1 and 2.The contralateral renal weight was measured by electron balance.The apoptosis in the contralateral renal cortex and medulla was detected by FITC-AnnexinV/PI flow cytometric assay.The morphological changes of the samples were also studied.Results After 4 weeks,the contralateral renal weight of the rabbits in groups 1,2 and 3 was(3.96?0.20),(3.47?0.19) and(3.20?0.21)g/kgBW,respectively.The difference of contralateral renal weight between groups 1,2 and group 3 was significant(P0.05).In contrast,the apoptosis rates of contralateral renal medulla cells in groups 1,2 and 3 were(23.75?2.34)%,(11.82?1.40)% and(2.36?0.65)%,respectively.There were significant differences between these 3 groups(P

Objective Selected the characteristic index of cervical CT tablets, and established stepwise regression equation, to explore the value of its application in forensic medicine identification. Method Each one cervical CT images of 130 cases were collected, 70 subjects were examined for 2 cervical CT images of each. Selected and measured 13 indicators of cervical CT iflms, the difference between the groups was calculated by the same measurement indicators after the random grouping of the inspectors, and the difference between the different measurement indexes of the same person. Using two classification logistic Stepwise regression analysis, establish the regression equation of the index and multiple regression equation of multinomial index, and the test of the equation was carried on blind test. Results Accuracy of 13 equations of the established element is 69.0%~90.4%,accuracy of 4 multiple regression equation is 90.4%~96.7%, blind accuracy rate is 100%. Conclusion 13 feature indicators selected from CT tablets can be used for the same identiifcation, in practice researchers should use multivariate index to get more accurate result.

ObjectiveTo observe the protective effect of high-dosage methylprednisolone (MP) after lumbar spinal stenosis operation. Methods20 Patients with lumbar spinal stenosis accepted laminectomy. The treatment group (11 cases) received MP 30 mg/kg when the skin incised, and followed with MP 40 mg twice a day for 3 d after operation. The control group (9 cases) received MP 40 mg twice a day for 3 d after operation. They were assessed with JOA scale before and after operation. ResultsThe JOA score was (9.25±2.12) and (9.53±2.10) in treatment group and control group before operation (P>0.05), and was (13.43±2.01) and (11.21±2.13) 1 week after operation (P<0.05), (14.62±2.15) and (13.04±2.11) 3 months after operation (P<0.05). The neurological symptom deteriorated in 1 cases of control group. No peptic ulcer, poor wound healing or other associated complications has been found. ConclusionHigh-dosage methylprednisolone may protect the spine cord intraoperative the lumbar spinal stenosis.

Objective To evaluate the value of retroperitoneal laparoscopic adrenalectomy for benign adrenal tumors.Methods From March 2003 to May 2008,78 patients with adrenal tumors received retroperitoneal laparoscopic adrenalectomy in our hospital. During the operation,three trocars were introduced into the lumbar region to establish retroperitoneal space by blunt dissection with the camera. Afterwards,the tumor was removed by using a harmonic scalpel. Results The procedures were successfully completed in all the 78 cases without blood transfusion,the operation time ranged from 45 to 180 min (mean,90 min). Drainage tubes were removed 24 hours after the operation. And the patients were discharged from hospital in 3 to 5 days postoperation. One patient showed regional subcutaneous emphysema after the surgery and was cured spontaneously. The patients were followed up for 3 to 24 months with a mean of 10 months,during which 36 patients achieved normal blood pressure in 3 months,while in the other 7 patients,oral antihypertension drugs were still needed. No patients had recurrent tumor during the follow-up. Conclusions Retroperitoneal laparoscopic can be the first choice for adrenal tumors,as it is safe and effective with minimal invasion,quick recovery,and short postoperative hospital stay.

Objective To study the role of phosphorylation at Serine 129 in regulating the neurotoxicity of ?-synuclein. Methods Wild type and phosphomimic mutant ?-synuclein genes were over-expressed in mouse dopaminergic cells MN9D using retrovirus. The cell viability was examined using CCK-8 assay and cell morphology was observed by immunofluorescence microscopy. Results The result of real time PCR showed that WT/?-SYN and S129D/?-SYN genes were overexpressed in MN9D as compared to uninfected MN9D and vector control group(P

Objective To analyze the relationship between pulse wave velocity (PWV)in young and middle-aged normal body mass patients with mild -moderate hypertensive and risk factors of cardiovascular diseases,and to explore the main factors influencing large arterial elasticity.Methods PWV were measured by Complior (Colson, France).Systolic blood pressure (SBP)and diastolic blood pressure were measured and fasting blood glucose,blood lipid were tested at the same time.The results was analyzed by correlation and multiple regression method.Results (1)Patients with hypertension had statistically significant higher cfPWV than nonhypertensive cases(P <0.01).(2) Correlation analysis showed that the cfPWV were positively related to age,systolic pressure,diastolic pressure,pres-sure difference(r =0.401,0.467,0.289,0.278,P =0.001,0.000,0.020);Deleting the factors of age,abdomen circumference and BMI,the cfPWV was still positively related to systolic pressure,diastolic pressure and pressure differences(r =0.417,0.297,0.315,P =0.001,0.018,0.012).Multiple regression analysis showed that age,systol-ic pressure were mostly related to cfPWV(t =3.945,3.481,P =0.000,0.001).Conclusion Artery elastic function in young and middle aged people is obviously lower in patients with hypertension.cfPWV has strong positive relation with age and systolic pressure.

Objective To provide accurate bone tunnel positioning reference for clinical individual anterior cruciate ligament (ACL) reconstruction by three-dimensional (3D) reconstruction of femoral and tibial original footprint of ACL.Methods Forty volunteers were selected for this study,including 20 males and 20 females,and aged 18 to 35 years old.3D CT scanning images of knee data were obtained.3D bone models of knee and footprint of ACL were reconstructed.On the 3D reconstruction models,the shape and position of original footprint of ACL were displayed.Statistical significance was compared for their difference.Results Femoral original footprint of ACL,long axis of footprint,distances to the distal margin of the articular cartilage (DDCM),distances to the posterior margin of the articular cartilage (DPCM),double bundle center distance,and tibial original footprint short axis of ACL were significantly different between genders (P ＜ 0.05).However,no significant difference was found between genders for tibial original footprint long axis of ACL (P ＞ 0.05).Conclusions The insertion mark shape and nature position of footprint of ACL could be observed by 3D reconstruction using CT scan images.

Objective To investigate the expression differences of MiR-34a/c in lesions of psoriasis vulgaris Uyghur and Han patients and beside-lesional tissue and normal skin tissue in XinJiang.Methods Real-time fluorescent quantitative polymerase chain reaction (PCR) was used to determine the expression levels of MiR-34a/c in the psoriasis vulgaris Uyghur and Han patients with lesion tissues, beside-lesion tissues, and normal skin tissues, respectively.Results The expression level of MiR-34a was higher in lesions of psoriasis vulgaris in XinJiang than in the beside-lesion tissues and normal skin tissues, and differences were statistical significance (P ＜ 0.05).However, the expression level of MiR-34a in beside-lesion tissues and normal skin tissues, and in the Uyghur and Han psoriasis vulgaris werent statistically significant difference (P ＞ 0.05).The expression level of MiR-34c in three types of tissues and in the Uyghur and Han psoriasis vulgaris weren't statistically significant difference (P ＞ 0.05).Conclusions MiR-34a might participate in the pathogenesis of Uyghur and Han psoriasis vulgaris in XinJiang.

Objective To investigate the effect of continuous positive airway pressure (CPAP) on seizure frequency in epilepsy patients complicated with obstructive sleep apnea (OSA). Methods Subjects were divided into CPAP group (20 subjects) and medication group (22 subjects) according to whether they can tolerate CPAP. CPAP group were treated with CPAP combined with antiepileptic drugs. Medication group were treated with antiepileptic drugs alone. Seizure frequency and apnea-hypopnea index (AHI) were compared between groups before and after 4 weeks and 24 weeks of treatment. Results Baseline seizure frequency and apnea-hypopnea index (AHI) were compared between groups(P>0.05). A sig?nificant reduction of seizure frequency was observed in CPAP group after 4 weeks and 24 weeks of treatment compared to that before treatment (P<0.01). In medication group, no significant difference in seizure frequency was noted betweem 4 weeks and 24 weeks after treatment compared to that before treatment(P>0.05). A significant reduction of AHI was ob?served in the CPAP group after 24 weeks of treatment compared to that before treatment (P<0.01), but no change of AHI by treatment was observed in medication group (P>0.05). Conclusion Treatment of OSA in patients complicated with epilep?sy may improve seizure control in short and long term, but longer observation time and more samples are needed for further research.