1.Tumor suppressive properties of maspin and its expression levels in tumors
Cancer Research and Clinic 2009;21(2):135-137
Maspin (mammary serine protease inhibitor) is a kind of serpin.Many studies have characterized it as a tumor suppressor based on its ability to inhibit tumor angiogenesis,increase adhesion between tumor cells and ECM,inhibit tumor cell invasion and distant metastasis and promote apoptosis.In recent years,some studies have found maspin expresses high in some cancers,such as in mammary cancer and prostate cancer,while in some other cancers,such as in pancreatic cancer,maspin expression level shows high.The expression levels and biological functions of maspin in different organs still need further study.
2.Measurement of Morphological Parameters of Cryptosporidium murisOocysts by Digital Image Processing Technology
Yuyan BING ; Yan LI ; Chunli AN
Chinese Journal of Parasitology and Parasitic Diseases 1987;0(03):-
Objective To establish and analyze the morphological parameters of the oocysts of Cryptosporidium muris for defining their morphological change. Methods Oocysts were collected from KM mice(immunodepressed by dexamethasone for 10 days) and examined with modified acid-fast staining. Images of 1 190 oocysts were acquired by photograph system. The length, width, perimeter, area and equivalent diameter of the oocysts were obtained by computer digital image processing system and analyzed by SPSS software (Version 11.0). Result The average length of the oocysts was 5.93 ?m, ranging from 3.36 ?m to 8.51 ?m in 95% confidence interval of them. The average width was 4.96 ?m, ranging from 3.26 ?m to 6.66 ?m in 95% confidence interval of them. The average perimeter was 18.03 ?m and the average area was 16.08 ?m2. Conclusion Data obtained from the computer system are objective and precise, offering scientific foundation for measuring the oocysts and for identifying Cryptosporidium spp.[
3.Optimization of Processing Technology of Prepared Rhubarb by Response Surface Method
Chunli CUI ; Bei WANG ; Chong DENG ; Yan SUN ; Yonggang YAN
Chinese Journal of Information on Traditional Chinese Medicine 2014;(9):98-101,102
Objective To screen the best processing technology of prepared Rhubarb by multi- component composite score combined with response surface.Methods The composite score of seven components, i.e., gallicacid, chrysophanol-8-O-β-D-glucopyranoside, aloeemodin, rhein, emodin, chrysophanol, physcion, was taken as indicators. Three factors and three levels of response surface Box-Behnken were used in experimental design. The effects of factors including rice wine volume, moistening time, and steaming time on processing technology of stewed Rhubarb were studied, and the optimized processing parameters were chosen.Results Design Expert 8.05 software was used for data analysis. Multivariate quadratic mathematical model of the sample and examine factors composite score regression equation of seven ingredients content were set up. The best processing conditions of prepared Rhubarb were as following:adding 36.6 mL rice wine into per 100 g raw material;moistening time continued for 2.16 hours;steaming time continued for 10.96 h. According to actual production, the optimized processing conditions of prepared Rhubarb are as following:adding 35 mL rice wine per into 100 g raw material;moistening time continued for 2 h;steaming time continued for 11 h.Conclusion The optimized processing technology can reasonably control the quality of prepared Rhubarb, and provide the basis for the quality standards of its decoction pieces and clinical application.
4.Effect of fluvastatin on induction and differentiation of human promyelocytic leukemia HL-60 cells
Liyan ZHAO ; Yan SHI ; Chunli SONG ; Yufen JIN ; Li XU
Journal of Jilin University(Medicine Edition) 2006;0(03):-
Objective To observe the effect of fluvastatin(Flu) on differentiation and induction of human promyelocytic leukemia HL-60 cells and provide the theoretical foundation for treatment of human promyelocytic leukemia.Methods The cultured HL-60 cells were treated with 20 ?mol?L-1 Flu,the morphological changes of the cell differentiation were examined.The NBT reduction capability was detected in HL-60 cells treated with Flu for 72 h.After HL-60 cells were treated with 20 ?mol?L-1 Flu for 5 d,they were stained with non-specific esterase and the percentage of differentiation cells was analyzed.Results The HL-60 cells treated with Flu showed smaller cell body,reducing proportion of nucleus to cytoplasm,the nucleus tortuosity,fold or sublobe.There were specific granules and vacuoles in cytoplasm,displaying that some cells had differentiated to relative mature cells.As compared with control group,the NBT reduction capability in HL-60 cells treated with Flu for 72 h was significantly higher than that in control cells(P
5.Content Determination of Shikonin in Arnebia euchroma by HPCE
Yan BAI ; Fuhai WU ; Bilian LUO ; Chunli ZENG
China Pharmacy 2005;0(19):-
OBJECTIVE:To determine the content of shikonin in Arnebia euchroma by HPCE-UV.METHODS:The separation was performed on uncoated fused silica capillary column (53 cm?50 ?m ID,46 cm effective length).2.0 mmol?L-1 H3BO3,6.0 mmol?L-1 triethylamine buffer solution and 5.0 mmol?L-1?-CD was used as background electrolyte,14 kV separation voltage and time of gravity injection 15 s (25 cm).The detection wavelength was set at 516 nm.RESULTS:The linear range of shikonin was 7.2~36.0 ?g?mL-1(r=0.999 0) with an average recovery of 100.15%(RSD=1.68%,n=6).CONCLUSION:The method is convenient,rapid,accurate and reliable for the content determination of shikonin in A.euchroma.
6.Preparation and characterization of aqueous polymer dispersions with high flexibility for coating
Yan YANG ; Jiabi ZHU ; Chunli ZHENG ; Shengjun CHEN
Journal of China Pharmaceutical University 2009;40(4):310-315
Aim: To prepare novel aqueous polymer dispersions with high flexibility for sustained-release coating and investigate their properties. Methods: The aqueous polymer dispersions were synthesized by the emulsion polymerization. The physico-chemical properties and film-forming potential of the dispersions were investigated while the mechanical properties of the formed film and the drug release behavior when atenolol pellets were coated with the aqueous polymer dispersions were evaluated. Results: The prepared aqueous polymer dispersions (methyl methacrylate/ethyl acrylate, 1:2) were found to have proper physico-chemical properties, excellent film-forming capability and satisfying mechanical properties. It could form free film with high flexibility and low viscosity in low temperature even in absence of the plasticizer. Sustained release of atenolol pellets was achieved when the pellets were coated the polymer dispersions. 4-h and 8-h cumulative releases were more than 50% and 80%, respectively. There was no significant difference in release between pellets prior to and post compression of the coated pellets. Conclusion: The resulting aqueous polymer dispersions could be used as sustained-release coating material with high flexibility suitable for tableting.
7.Improvement and evaluation of modeling method in animal models of osteoporosis
Chunli ZHANG ; Zhonghai LI ; Ying ZHOU ; Yan LIU ; Shuxun HOU
Chinese Journal of Tissue Engineering Research 2016;20(5):754-759
BACKGROUND:Animal models of osteoporosis play an important role in the research of the pathogenesis, occurrence and development of osteoporosis, as wel as in the clinical diagnosis, prevention and treatment of osteoporosis. OBJECTIVE: To summarize and discuss the establishment and research ideas of osteoporosis models, explore the current situation and advance of osteoporosis models, compare the advantages and disadvantages of various methods, and provide evidence for clinical investigation. METHODS: A computer-based online search was conducted in SinoMed, VIP, Wanfang and PubMed databases by using the key words of “animal model, osteoporosis” from January 1969 to October 2015. The language was limited to both Chinese and English. Relevant articles were screened according to inclusion and exclusion criteria. The documents about the methods of osteoporosis model preparation, method improvement as wel as their advantage and disadvantage were summarized. RESULTS AND CONCLUSION:A total of 576 articles were included. Among them, articles published earlier, duplicated, and similarly were excluded, and 53 articles were finaly included. Various animal models of osteoporosis may only focus on the certain causes, certain stage, some of the main symptoms and some pathophysiological changes of disease. Accordingly, appropriate modeling methods and experimental animals should be selected based on research objective. Rat undergoing castration is the most commonly used model in the modeling of osteoporosis. Among drug methods for constructing osteoporosis model, glucocorticoids is the most commonly used one. Disuse method and nutritional method have limitations, and always combined with castration and drug methods. The effects of gene transfer, gene mutation and brain-derived model deserve
8.Clinical study on anti—SSA antibody by using dot immunobinding assay
Ming JING ; Shujie ZHOU ; Zhongchen KOU ; Chunli YAN
Chinese Journal of Immunology 1985;0(05):-
Using human spleen purified SSA antigen,we set up a new technique of dot immunobinding assay(DIBA)for detection of anti—SSA antibody.The specificity of DIBA is better than that of double immunodiffusion method.The positive rate of anti—SSA antibody in Sjogren syndrome is 76.9%,while that in systemic lupus erythematosus is 33.3%.We found that anti—SSA antibody and RF usually appeared in same patient with Sjo-gren syndrome or rheumatoid arthritis.
9.Investigation of skin prick test on patients with allergic rhinitis in Urumqi area.
Chunli WANG ; Hua ZHANG ; Xinping CHEN ; Yan ZHENG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(10):718-720
OBJECTIVE:
To investigate the geographic distribution of air-borne allergens which caused allergic rhinitis in Urumqi, and offer guide for prevention and treatment of the patients with allergic rhinitis.
METHOD:
Skin prick tests were performed on 350 patients with allergic rhinitis by assay of Novo-helisen depot (NHD) fluid.
RESULT:
The total positive rate of inhaled allergens among 350 patients was 78.86%. The most frequent of allergen was chenopodium 78.86% (255 cases). The other frequent allergens were ragweed 36.86% (128 cases), artemisir 28% (98 cases), timothy 27.14% (95 cases), elm 5.71% (90 cases), willow 25.14% (88 cases), poplar 18.86% (66 cases), cockroach 18.86% (66 cases), dust-mite 14.86% (62 cases), flour mite 14.86% (52 cases), alternaria spp 2.86% (10 cases). Allergen were multiple in most AR patients. There was not statistically significant difference between the sexes. There was statistically significant difference between the tribes.
CONCLUSION
Chenopodium, ragweed and artemisir were the main allergens in patients with allergic rhinitis in Urumqi.
Adolescent
;
Adult
;
Aged
;
Allergens
;
analysis
;
Child
;
Child, Preschool
;
Female
;
Humans
;
Male
;
Middle Aged
;
Rhinitis, Allergic
;
immunology
;
Skin Tests
;
Young Adult
10.To Strengthen Information Analysis and Evaluation Competency——On the Education of Information Literacy of Medical Students
Hong HU ; Wenlong ZHAO ; Chunli LI ; Bo LV ; Yan HE
Chinese Journal of Medical Education Research 2005;0(05):-
As society develops,it has become one of the important goals of higher medical personnel training to cultivate medical students' information literacy.After explaining the definition of information literacy,this paper discussed the importance of information analysis and evaluation in information literacy education.