Objective To investigate the drug sensitivity of cardia tumors in vitro by ATP-TCA, and to provide basis of clinic chemotherapy. Methods 42 cardia tumors were tested by ATP-TCA to determine and assess the drug sensitivity of 8 common chemotherapeutics. Correlation analysis was done between the clinical classification and the drug sensitivity. Results The drug sensitivity had significant difference among cardia tumors. From high to low, it was ADM>MMC>PTX> 5-Fu>HCT =VP16>CDDP>IFO, Sensitive degree were 50.00 %, 35.45 %, 31.25 %, 23.53 %, 10.53 %, 10.53 %, 7.69 %, 5 %. Sensitivity of the same drug in different pathologic stages had no significant difference. Conclusion ATP-TCA can play a guiding role in individual chemotherapy for human cardia cancer.

Seventeen physiological,biochemicaland morphological indexes consistently showed that Astragalus polysaccharide was able to enhance the myocardia contractility,attenuate the area of myocardial infarction, and reduce the degree of myocardial damage of myocardially infarcted dog heart in vivo.The mechanism might be related to the inhibition of Na+-Kr+ATPase activity & the anti-free-radical-damage action.

Objective To compare the perioperative outcomes of laparoscopic liver resection (LLR) versus open liver resection (OLR) for hepatocellular carcinoma (HCC).Methods A total of 89 HCC patients undergoing liver resection between January 2012 and November 2016 were enrolled.Nonparametric tests were employed to compare the clinicalpathological characters and preoperative outcomes.Results No significant difference was observed in clinicalpathological features and postoperative morbidity.LLR group had shorter hospital stay (Z =4.642,P ＜0.01),lower serum ALT level in 1st,3rd and 5 day (Z =2.157,3.089,2.384,all P ＜0.05) and AST level in 1st-and 3rd-day postoperatively (Z =2.688,2.566,all P ＜0.05).The growth rate in serum total protein (TP) and albumin (ALB) postoperatively is higher for LLR group (y =2.348 4x + 51.696 vs.y =0.902 9 + 35.532),(y =1.539 9x + 29.68 vs.y =0.732 9x + 30.406).Conclusion LLR allows quicker liver function recovery and shortens patients' postoperative hospital stay.

Objective To explore perioperative nursing of excision with vertical and horizontal incision and repair of palpebral margin pigmented nevus.Methods Ten patients with palpebral margin pigmented nevus excision of vertical and horizontal incision and repair were selected as research objects,perioperative nursing and effect of palpebral margin pigmented nevus excision.Results The pathological examination showed it was benign lesions and postoperative incision of 10 patients was successful with eyes symmetrical in shape,normal eyes'open and close function,no complications,and no deformity.After followed up for 1 year after operation,there was no recurrence and obvious scar.Conclusion Excision with vertical and horizontal incision and repair of palpebral margin pigmented nevus is characterized by smaller wound surface,less complications,and better postoperative recovery.And it is crucial for success of surgery and postoperative recovery to carry out perioperative nursing.

Objective To explore perioperative nursing of excision with vertical and horizontal incision and repair of palpebral margin pigmented nevus.Methods Ten patients with palpebral margin pigmented nevus excision of vertical and horizontal incision and repair were selected as research objects,perioperative nursing and effect of palpebral margin pigmented nevus excision.Results The pathological examination showed it was benign lesions and postoperative incision of 10 patients was successful with eyes symmetrical in shape,normal eyes'open and close function,no complications,and no deformity.After followed up for 1 year after operation,there was no recurrence and obvious scar.Conclusion Excision with vertical and horizontal incision and repair of palpebral margin pigmented nevus is characterized by smaller wound surface,less complications,and better postoperative recovery.And it is crucial for success of surgery and postoperative recovery to carry out perioperative nursing.

This investigation was aimed to explore whether over-expression of 27heme oxygenase-1 (HO-1) could protect bone marrow mesenchymal stem cells(BMSCs)against injury induced by high-concentration glucose. We cultured BMSCs in high-concentration glucose medium, and up-regulated or inhibited HO-1 expression in BMSCs through its agonist or inhibitor. We detected the ability of BMSCs proliferation and secretion respectively by MTT and enzyme-linked immunosorbnent assay (ELISA). Then we detected the effect of BMSCs conditions medium on proliferation and migration of human umbilical vein endothelial cells (HUVECs) through scratch experiments and transwell assay. It was found that HO-1 over-expression could not only promote BMSCs proliferation, but also promote secretion of vascular endothelial growth factor (VEGF), and could further accelerate the proliferation and migration of HUVECs. It could be well concluded that HO-1-over-expressing BMSCs can not only inhibit damage induced by high-concentration glucose, but can promote the proliferation and migration of vascular endothelial cells through paracrine as well. The result indicated that HO-1-over-expressing BMSCs played an important role in the treatment of diabetic vascular complication.
Cell Movement ; drug effects ; Cell Proliferation ; drug effects ; Cells, Cultured ; Culture Media, Conditioned ; pharmacology ; Glucose ; toxicity ; Heme Oxygenase-1 ; metabolism ; Human Umbilical Vein Endothelial Cells ; cytology ; Humans ; Mesenchymal Stromal Cells ; cytology ; Up-Regulation ; Vascular Endothelial Growth Factor A ; metabolism

Objective To explore the determinants of serum uric acid (UA) levels and the relationship between UA and cardiovascular disease in elderly patients.Methods A cross-sectional design was used.A total of 1 066 elderly patients were consecutively recruited in the study.Anthropometric measurement and lifestyle survey were performed,and serum UA,lipid profile,glucose,homocysteine (Hcy) and superoxide dismutase (SOD) were measured.The determinants of serum UA levels and correlation between UA and cardiovascular disease were analyzed by regression.Results The prevalence of hyperuricemia was 21.9％ (25.9％ in men and 18.7％ in women).Partial correlation analysis showed the level of serum UA was positively correlated with Hcy (r=0.163,P=0.000),body mass index (r=0.128,P=0.004) and triglyceride (r=0.133,P=0.003),and negatively correlated with HDL-C (r=-0.103,P=0.021).After adjustment for potential confounding factors,multivariate analysis showed eGFR (β =-2.044,t =-10.544,P =0.000),gender (β =42.065,t=4.700,P=0.000),Hcy (β=1.367,t=3.714,P=0.000),BMI (β=3.370,t=2.706,P=0.007),TG (β=14.120,t=2.589,P=0.010) and SOD (β=-0.636,t=-3.079,P=0.002) were independent determinants for UA levels in elderly patients.Logistic regression analysis indicated that mild elevation of UA levels was a risk factor of hypertension (OR=1.925,95％ CI=1.124-3.295) in women and OR=1.780 (95％ CI=1.010-3.136) in men].High UA levels increased the risk of coronary heart disease in women [OR=1.710 (95％ CI=1.157-2.526)],but decreased the risk of ischemic stroke in men [OR=0.524 (95％ CI=0.335-0.820)].Conclusions In elderly patients,serum UA levels were affected by renal function,gender,BMI and serum Hcy,TG and SOD.Mildly elevated UA levels increased the risk of hypertension.High UA levels increased the risk of coronary heart disease in women and decreased the risk of ischemic stroke in men.

OBJECTIVE: To explore the correlation among coping style, social support, and negative emotion in Chinese infertile women. METHODS: A total of 211 infertile women was enrolled for this study. Participants completed questionnaires including Social Support Rating Scale (SSRS), Simplified Coping Style Questionnaire (SCSQ), Selfrating Anxiety Scale (SAS), and Self-rating Depression Scale (SDS). RESULTS: The mean scores of the 2 dimensions of SCSQ for the infertile women were 20.18±5.43 (positive coping) and 10.19±3.83 (negative coping), respectively. The total mean score of SSRS was 38.95±6.87, and the mean score of 3 dimensions of SSRS were 22.63±4.15 (subjective support), 8.97±2.73 (objective support), and 7.35±1.87 (extent of using the support), respectively. The mean scores of SAS and SDS were 43.44±10.45 and 50.06±10.59, respectively. SAS scores were negatively correlated to the scores of positive coping, subjective support, objective support and extent of using the support (P<0.01) while positively correlated to the scores of negative coping (P<0.01). SDS score were negatively correlated to the scores of positive coping, subjective support, objective support, and extent of using the support (P<0.01). SEM analysis showed that positive coping had direct and positive effect on social support (β=0.49, P<0.01) while direct and negative effect on negative emotions (anxiety and depression, β=-0.27, P<0.01). Negative coping had direct and negative effect on social support (β=-0.21, P<0.01) while direct and positive effect on negative emotions (β=0.21, P<0.01). Positive coping and negative coping also had indirect effects on negative emotions through the pathway of social support, which had direct and negative effect on negative emotions (β=-0.21, P<0.01). CONCLUSION Coping styles have direct and indirect effects on negative emotions for the infertile women. As a mediator, social support regulates the relationship between coping styles and negative emotions. Using positive coping more frequently while using negative coping less frequently can alleviate the negative emotions of the infertile women through improving social support levels directly or indirectly.
Adaptation, Psychological ; Adult ; China ; Emotions ; Female ; Humans ; Infertility, Female ; psychology ; Social Support ; Surveys and Questionnaires

Objective To investigate the demand for perioperative health education in liver transplantation patients at different stages, and to discuss its influencing factors, so as to provide the empirical evidence for nurses to satisfy patients' health education demand. Methods From July 2014 to March 2016, the self-designed health education demand questionnaire for patients undergoing liver transplantation was used to investigate 71 patients who met the criteria. Single factor analysis and multiple regression analysis were used to explore the influencing factors of the health education demand. Results The score of health education demand in liver transplantation patients was (114.48±5.27), which was at a relatively high level. The item with highest demand at preoperative stage was the financial burden of the surgery, with the score of (4.52±0.81). Diet guidance was the highest at postoperative stage, and the score was (4.61±0.57). Before discharge, review follow-up guidance was the most popular item, with the score of (4.72±0.45). Multiple regression analysis demonstrated that education background and waiting time for liver were the main influencing factors of health education demand (P<0.05). Conclusions The nursing staff should realize the current situation of health education demand in liver transplantation patients and provide individualized health education for each individual patient to improve the patients' quality of life.

Objective? To explore the application effect of online learning platform on the training of family caregivers of disabled elderly people. Methods? From September 2017 to March 2018, the family caregivers of 90 disabled elderly people in a district of Changsha were selected by convenience sampling method, and divided into control group and observation group by random number table method, with 45 cases in each group. During the study period, the caregivers of the control group received general health education. The caregivers in the intervention group received online training on the self-developed online learning platform under the guidance of the researchers for months. Before and after the training, the family caregivers were investigated using the Family Caregivers Care Ability Scale and the Care Knowledge Assessment Questionnaire. Results? Before the training, there was no statistically significant difference in average score of care knowledge, and the total score and the five dimensions' scores of the Family Caregivers Care Ability Scale between the two groups (P ＞ 0.05). After training, the score of the care knowledge in the intervention group was (82.2±15.2), which was higher than that in the control group (64.2±17.2), and the difference was statistically significant (t=5.260,P＜ 0.01). After training, the score of Family Caregivers Care Ability Scale in the intervention group was (13.90±4.52), which was lower than that in the control group (16.57±3.25), and the difference was statistically significant (t=3.217,P＜ 0.01). The scores of the four dimension of the scale, including adjusting the role of caregiver, meeting need and providing assistance, dealing with personal mood, adjusting life in order to meet the needs to take care of elderly in the intervention gorup were lower than those in the control group, and the differences were statistically significant (P＜ 0.05). Conclusions? The training based on the online care knowledge and skill system for disability elderly can improve the family caregivers of nursing knowledge level and ability to take care of the elderly.