Objective:To compare the estimation of glomerular filtration rate(GFR)by renal dynamicimaging with that estimated by two-sample method and evaluate the reliability of measurement of GFR byrenal dynamic imaging.Methods:The study was performed in 54 patients(33 males and 21 females;aged 15 to 82 years)with primary or secondary chronic nephrosis.The patients were intravenously bolusinjected with 185 MBq/1 mL of (99)~Tc~m-DTPA 20 min after drinking 300 mL of water.Renal dynamic ima-ging was performed and the image was processed according to standard procedure(Gates' method)to ob-tain GFR.3mL of blood was withdrawn 2h and 4h postinjection,respectively,and radioactivity of 1mLplasma was measured.GFR was calculated by the formula of two-sample method.The correlation ofGFRs measured by renal dynamic imaging and by two-sample method was analyzed using regression anal-ysis.The correlations of GFRs determined by the two methods with urea and creatinine were also meas-ured.Results:The GFR measured by renal dynamic imaging was slightly lower than that measured bytwo-sample method.Excellent correlation was obtained by two methods with r=0.881 5 and P

Objective To construct eukaryotic expression vector of attenuated Salmonella typhimurium containing hCTLA-4Ig cDNA and identify its expression in COS-7 cells for the further study of function in SLE models. Methods Touchdown PCR was used to amplify hCTLA-4Ig cDNA.The PCR product was ligated into the multiple clone site of eukaryotic expression vector pcDNA3.1(+) by gene recombination technique.Then the recombinated plasmid pcDNA3.1(+)-CTLA-4Ig was transfected into COS-7 cells using DOTAP.The expression of interest protein in the supernatant of the cell disruption was detected with SDS-PAGE and Western blotting.Results Restriction analysis and DNA sequence analysis showed that the CTLA-4Ig cDNA had been successfully inserted into pcDNA3.1(+) eukaryotic expression vector.The interest protein could be detected in the supernatant of cell disruption 48h after the transfection of pcDNA3.1(+)-CTLA-4Ig.This protein specifically bound with human CTLA-4 monoclonal antibody.Conclusion The eukaryotic expression vector containing hCTLA-4Ig gene was successfully constructed and bioactive interest protein could be successfully expressed in mammalian cells.

Objective:To investigate whether PLC? gene down regulation by RNA interference(RNAi) leads to inhibition of proliferation in human bladder carcinoma T24 cell.Methods: The shRNA recombinant plasmids targeting to PLC? gene was constructed and transfected into bladder carcinoma T24 cell with Lipofectamine 2000.RT-PCR was used to monitor the validity of specific s h R N A in down regulation of PLC?.Then MTT assay was performed for detecting cell proliferation,the changes of PCNA were analyzed by immunocytochemical method,Electron microscope was used to observe the morphological changes.Results: The specific PLC? shRNA was confirmed to be efficient in silencing PLC? expression.PLC? gene down regulation by the shRNA recombinant plasmids inhibition cell proliferation rate about 78.01%,They were significantly different from that of control group(P

Objective To compare the clinical efficacy of carvedilol and metoprolol in the treatment of dilated cardiomyopathy patients with heart failure (DCM).Methods According to the different treatment method,90 DCM patients with heart failure patients were divided into the observation group and the control group,45 cases in each group.Both groups were given a diuretic,vasodilator,cardiac and other drug treatment,and the control group were added with metoprolol,and the observation group were added with carvedilol.The treatment lasted 4 to 6 months.Before and after treatment,the levels of left ventricular ejection fraction(LVEF),left ventricular end-systolic diameter (LVESVI),left ventricular diastolic diameter (LVEDVI),left ventricular fractional shorteningrate (FS),largest peak velocity (hCFV),coronary flow reserve (CFR),simultaneous detection of serum levels of endothelin (ET) and nitric oxide(NO) content were observed and compared betweon the two groups.Results After the treatment,the levels of LVEF,LVESVI,LVEDVI,FS,hCFV,CFR,ET,NO of the observation group were (44.0 ± 3.4) ml/m2,(45.2 ±6.9)mm,(20.9 ±5.4)mm,(30.4 ±5.3),(78.2 ±31.6) cm/s,(3.12 ±0.36),(52.2 ±5.6) ng/L,(5.2 ± 0.67) μmol/L,respectively,which were better than those of the control group[(37.9 ± 3.5)mL/m2,(51.1 ± 7.6)mm,(20.7 ±6.3) mm,(25.2 ±7.3),(70.5 ± 31.8)cm/s,(2.85 ±0.23),(63.5 ± 1.8)ng/L,(2.55 ± 0.33) μmoL/L] (t =8.386,-3.856,-4.487,3.867,-1.152,-4.239,12.887,-23.802,all P ＜ 0.05).Conclusion Carvedilol is an effective drug for the treatment of DCM consolidated psychological failure,which can improve heart function and coronary flow reserve capacity and endothelial function in patients,which is worth in the clinical with satisfactory results.

Post-transplant lymphoproliferative disorders (PTLD) is one of the most potentially lifethreatening complications after allogeneic hematopoietic stem cell transplantation (a]lo-HSCT),and is mostly associated with Epstein-Barr virus infection.PTLD may have a wide range of clinical manifestations and pathological classifications.The rapid progression and high fatality of early PTLD should prompt clinicians to a timely diagnosis.This paper presents a review of the latest update on epidemiology,pathogenesis,clinical manifestations,diagnosis,management and prognosis of post allo-HSCT PTLD.

Objective:Establishing a simple useful method of the ultraviolet spectrophotometric to determine the arteannuin element content,providing the fast basis for screening and shopping high quality arteannuin.Method:Carrying on the wave length scanning with the ultraviolet spectrophotometer to arteannuin element standard solution, establishing the standard curve, according to standard curve determination sample content. Results:Arteannuin element most suitable absorption wave length is 290 nm, and the extinction-density regression equation is:Y=0.043X+0.006. The different arteannuin element sample content has the difference.Conclusion:The method of ultraviolet spectrophotometric determining arteannuin element content operation is quickly,simple and accurate.

Objective:To investigate the proliferation inhibitory effect of crocin on transitional cell carcinoma of urinary bladde(rTCCB). Methods:MTT assay was used to evaluate the inhibitory effect of Crocin on the proliferation of T24 cells. Microscope was used to observe the morphological changes of the T24 cells. Flow cytometry was used to measure the cell cycle. T24 cells were inoculated in to BALB/c nude mice to establish bladder cancer model. After treatment with Crocin,the inhibitory effects were observed on the growth of tumors. Results:The proliferation of T24 cells were inhibited remarkably. Morphological changes of cells were observed. Flow cytometric profiles revealed that crocin led to the increase of the cells in G0/G1 phase and the decrease of cells in S phase and G2/M(P

OBJECTIVE:To survey the status quo of ADR occurred in our hospital.METHODS:654 ADR reports were collected from our hospital during 2003～2009 and analyzed statistically.RESULTS:Of total 654 ADR cases,the ratio of male to female was 1∶1.67.20.34%of ADR occurred to people aged from 41 to 50.452 ADR cases were induced by intravenous administration accounting for 69.11%and 177 ADR cases were induced by oral administration,accounting for 27.06%.342 ADR cases were caused by antibiotics(52.29%),among which 127 cases by cephalosporins(37.13%),91 cases by quinolones(26.61%)and 54 cases by macrocyclic lactone(15.79%);92 cases were caused by cardiovascular system drugs(14.07%).Main clinical manifestations were lesion of skin and its appendants(25.54%)and digestive system injury(22.48%).CONCLUSION:Great importance should be attached to ADR monitoring,rational use of antibiotics and close monitoring of TCM injection.

Objective To study the expression of cytokeratin(CK)20 in exfoliated urothelial cells and to evaluate its clinical significance. Methods The expression of CK20 in exfoliated urothelial cells in 40 patients with bladder cancer and 20 subjects with normal urothelium was observed with immunohistochemical methods. Results The positive staining of CK20 in patients with bladder cancer was 52.5%, the expression of CK20 was negative in all controls, the positive rate of expression of CK20 combining with HE staining in patients with bladder cancer was 90%. The expression of CK20 was significantly higher in recurrent cases(80%) than in primary cases(43.3%). CK20 was not related to the tumor grade or stage. Conclusions The results suggested that the method had a higher specificity and therefore CK20 would be a good diagnostic marker.

The INK4a-ARF locus is located on CDKN2A point in human chromosome 9p21 and is known to encode two functionally distinct tumor-suppressor genes p16INK4a (MTS1/CDKN2/INK4a/p16) and p14ARF, have been shown to play significant roles in cell-cycle regulation in cancer. p16 is encoded by the exon 1a-exon 2-exon 3 transcript and functions as a negative regulator of the cell cycle through its inhibition of cyclin-dependent kinases (CDKs) 4 and 6 and subsequent blockage of the cyclindependent phosphorylation of pRB . Thus, loss of p16 function leads to deregulation of pRB's suppressive block of the G1-to-S transition and cell proliferation. In contrast, p14ARF is encoded by the exon 1?-exon 2 transcript and acts upstream of p53. Biochemical evidence has demonstrated that p14ARF physically interacts with human double minute 2 (HDM2) gene product and consequently stabilizes p53, leading to G1 cell-cycle arrest. Overall, somatic mutation of the INK4a/ARF tumor suppressor locus, resulting in functionally deficient p16 and possibly p14ARF proteins, seems to be a prevalent event in the development of oesophagus carcinoma.