Objective:To observe effects of electroacupuncture at Jiaji(EX-B_2)on phosphorylated extracellular signal regulated kinase(ERK)and neurokinin-1(NK-1)in dorsal horn of the complete Freund's adjuvant arthritis rat,so as to study possible mechanism of electroacupuncture analgesia from the point of view of signal conduction.Methods:Eighty Wistar adult male rats were randomly divided into normal control group,simple electroacupuncture(EA)group,model 30 min group,model 24h group,model 48 h group,EA 30min group,EA 24h group,and EA 48h group,10 rats in each group.Inflammatory pain rat model was established by injection of complete Freund's adjuvant into left hindpaw.EA was given at bilateral L_3～L_5 Jiaji(EX-B_2).The pain threshold was determined and phosphorylated ERK and NK-1 expressions in the spinal cord were detected by immunohistochemical technique.Results:The pain threshold significantly decreased(all P

To investigate the deletion of MTS1/p16 gene in human colorectal carcinoma,the homozygous deletion of MTS1/p16 gene was checked by reverse transcriptase polymerase chain reaction from 60 patients with colorectal carcinoma.The specimens consisted of 35 colorectal carcinoma,15 tumor-adjacent tissues and 10 normal colorectal mucosa. It was found that 17 14% (6/35) colorectal carcinoma specimens showed homozygous deletion of MTS1/p16 gene.No deletion was detected in tumor-adjacent tissue and normal colorectal mucosa.Deletion of MTS/p16 gene may play a role in the progression of colorectal carcinoma.

Objective To investitgate the level of polychlorinated biphenyls in retail livestock meat.Methods Totally 28 samples of three kinds of meat including of beef,pork and mutton were collected during Aug-Dec.,2008.Twelve dioxin-like polychlorinated biphenyls(DL-PCBs) and six marker PCBs were extracted by Soxhlet extractor,cleaned up by FMS and qualitative and quantitative analyzedsis by isotope dilution high-resolution gas chromatography/high resolution mass spectrometry(HRGC/ HRMS).Results Levels of total 18 compounds including of 12 DL-PCBs and indicator PCBs in beef,pork and mutton were 1 607.60 pg/g fat,435.82 pg/ g fat,and 505.17 pg/g fat respectively,DL-PCBs concentration were 456.19 pg/g fat,46.37 pg/g fat and 77.50 pg/g fat respectively.Maker-PCBs concentration were 1 151.41 pg/g fat,389.45pg/g fat and 427.67 pg/g fat respectively.The levels of PCB toxicity equivalent(TEQ)(the median value)were:0.84 pg/g fat in beef,0.15 pg/g fat in pork,0.28 pg/g fat in mutton respectively.The TEQ concentration of PCB(median value) was lower than the EU action level limit value.In all samples,PCB118 was the greatest contributor to the total contaminated concentration,and PCB126 was the dominant contributor to toxicity equivalent(TEQ).Six of 28 samples exceeded the limit value of the EU action level.Conclusion The results show that PCBs contamination exists in a city from the six samples exceeding the EU standard limit.It should take measures to explore the cause of contamination of PCBs compounds and control the pollution in order to guarantee food safety effectively.

Objective To evaluate the clinical value of cerebrospinal fluid ADA,IL-23 joint detection in the diagnosis of tuberculous meningitis related diseases.Methods 253 cases with tuberculous meningitis related diseases were selected as the research subjects.According to the diagnosis,they were divided into tuberculous meningitis group (meningitis group,138 cases ),tuberculous meningitis complicated with hydrocephalus group (hydrocephalus group,35 cases)and control group(80 cases).All patients after admission received lumbar puncture, the part of cerebrospinal fluid specimens were inspected,the cerebrospinal fluid ADA,IL -23 joint test was conducted.Meningitis group and hydrocephalus group were given anti -TB drugs (INH,RFP and PZA +sm ) combined with chemotherapy.3 months after treatment,the meningitis group and hydrocephalus group received lumbar puncture cerebrospinal fluid ADA,IL -23 joint test again.The levels of ADA,IL -23 in cerebrospinal fluid were compared.Results Before treatment,cerebrospinal fluid ADA,IL-23 levels in the meningitis group were (12.64 ± 5.54)u/L and (48.38 ±10.78)pg/mL,those in the hydrocephalus group were (15.81 ±6.92)u/L and (77.21 ± 13.42 mm)pg/mL,which in the control group were (3.21 ±2.20)u/L and (9.05 ±3.89)pg/mL,ADA,IL-23 levels in meningitis group and hydrocephalus group before treatment were significantly higher than the control group (F=117.24,724.97,P<0.001).Spearma analysis showed that each group of cerebrospinal fluid ADA and IL-23 had no correlation.After treatment,the cerebrospinal fluid ADA,IL-23 levels in the meningitis group were (3.79 ± 3.13)u/L and (13.46 ±6.62)pg/mL,which in the hydrocephalus group were (6.42 ±4.35)u/L and (25.42 ± 8.54)pg/mL,the meningitis group before and after treatment had statistically significant differences in cerebrospinal fluid ADA,IL-23 (t=16.34,32.43,all P<0.001);hydrocephalus group before and after treatment had statistically significant differences in cerebrospinal fluid ADA,IL-23 (t=6.80,19.26,all P<0.001 ).Conclusion Cerebro-spinal fluid ADA,IL-23 joint detection in the early diagnosis of tuberculous meningitis related diseases and clinical observation has high clinical value.

Objective To access the clinical value of interstitial brachytherpay in patients with advanced carcinoma. Methods 6 patients(1 case of hepatic cancer,3 cases of prostate cancer,2 cases of local recurrence of rectal cancer)received interstitial high dose rate(Ir-192)brachytherpay with afterloading technique(6.0 Gy/day in 4 days) Tubes were inserted with laparoscopic guidance to minimize the risk of tube misplacement. Results The size of the tumors was reduced and the local control rate was encouraging(6/6). one patient with local recurrence of rectal cancer aquired CR,the ofher five cases acquired PR. Conclusions Interstitial tube insertion with laparoscopic guidance to treat advanced carcinoma in afterloading technique is a simple,minimally invasive,safe and economical method,especially fit for elder patients who intolerate or disagree to operation.

Objective To evaluate the therapeutic efficacy of YunKe and prednisone on moderate and severe thyroid associated ophthalmopathy(TAO).Methods 1 08 hyperthyroidism patients with moderately severe TAO were randomly divided into YunKe group,hormone group and the control group.All of the patients were given anti -thyroid medication(ATD)treatment.By thyroid hormone level upper limit every times higher than normal for a sulfhydryl imidazole 5mg/day to calculate daily dosage,based on thyroid hormone level adjusted a sulfhydryl imidazole dose every two weeks,make thyroid hormone levels in the normal range.YunKe set:intravenous push note YunKe per day A +B agent four groups (including cloud 20mg),each treatment medicine for five days in A row,interval of 25 days to the next period of treatment,medication six procedures.Hormone group:oral prednisone for 6 months,monthly dose in the order 1 mg·kg -1 ·d -1 ,0.75mg·kg -1 ·d -1 ,0.5mg·kg -1 ·d -1 ,0.25mg·kg -1 ·d -1 ,0.1 25mg·kg -1 ·d -1 , 0.062 5mg·kg -1 ·d -1 ,1 time a day,took at 8 o'clock in the morning.The control group treated ATD.Before and 6 months after treatment,all of the patients were checked the TNF -αand TRab,CT scan to measure degree of exophthalmus.Six months after treatment,respectively,on the basis of TAO classification (NOSPECS)and TNF -α, TRab level changes to determine the efficacy of treatment.Data entry Excel 2003 statistical software,used SPSS 1 9.0 statistical software to analyze data.Non parametric tests of three groups of data with Kruskal Wallis test.Not matching the two independent samples nonparametric test with the Mann -Whitney test,with P <0.05 for the difference was statistically significant.Results The markedly effective rate,effective rate and ineffective rate of YunKe group were 36.1 %,47.2% and 1 6.7% respectively,which of the hormone group were 20.0%,37.1 % and 42.9%,which of the control group were 1 3.9%,22.2% and 63.9%.The efficacy among the three groups had statistically significant differences (Kruskal -Wallis χ2 =1 0.736,P =0.005 ).The lighter exophthalmos,tge curative effect was better (Mann -Whitney Z =-4.599,P <0.001 ).The changes of TNF alpha,TRab levels among the three groups had significant differences(YunKe group,the TNF alpha,TRab levels markedly reduced 75.0% and 80.6%,respectively, which of the hormone group were 37.1 % and 34.3%,which of the control group were 1 9.4% and 27.8%,TNF χ2 =23.527,P <0.001 ;TRab χ2 =23.831 ,P <0.001 ).Conclusion The therapeutic efficacy of YunKeon TAO is better than prednisone,but the long -term effect deserves more investigation.

In order to meet the requirements of logistic supply in peacetime and wartime, General Health Department developed Managerial and Maintenance System of Army Medical Equipments, which is the important subject of 10th 5-year Army Medical Research. This system is based upon Basic Medical Equipments Distribution Standard, Army Medical Equipments Management System, joint support system and three-level maintenance network. An efficient platform for whole-survival-circle medical equipments monitoring is developed using computer information technology. This system can greatly advance the informatized, normative management of medical equipments, and thus is of avail in military and economic aspects.

Objective To investigate the main causes and strategies for the difficulties in ureteroscopic lithotripsy.Methods From March 2004 to December 2006,19 cases of ureteral calculi,who experienced difficulties during holmium laser lithotripsy or pneumatic lithotripsy under a rigid ureteroscope,were analyzed retrospectively.Among the cases,3 had difficulties in ureteroscope placement due to the narrow ureteral ingress,6 owing to calculus obstruction complicated with ureteral inflammatory polypi,4 resulted from twisted ureter,and 6 because of stenosis of the ureter.Results In 15 of the patients,the operation was successfully performed by changing surgical approach,controlling the hydraulic irrigation,and incising the stenotic segments,etc.Two patients,who had stenotic ureter,received ESWL with double-J catheter dwelling.Lithotripsy failed in 2 cases,and PCNL was used to remove the calculi.The postoperative complications occurred in 4 cases,including 2 with mucosal laceration and 2 ureteral perforations.One of the 4 cases was transferred to an open surgery,and the other 3 were cured by conservative treatments.Conclusions Holmium Laser lithotripsy and pneumatic lithotripsy under a rigid ureteroscope are safe and effective in treating ureteral calculi.

[Objective] To investigate the curative effect of the red rice in treating the experimental fractures in the rats.[Methods] 64 Wistar rats suffered with single fracture in the forearm were randomized into two groups,one red rice group and the other normal saline control group.On the twenty-first day and 42 day after operation,the qualitation and morphometric observation of the bone tissue were performed.[Results] After the treatment of the red rice,the bony union of the fractured bone in the rats was obviously accelerated.The morphometric index of the bony tissue was higher than the control group with the statistical significance.The remodeling of the bony callus,the emergence of the lamellar bone and recanalization of the medullary cavity could all be enhanced by the use of the red rice.The mineralized bony callus area,the osteoblast index and the matrical calcification were obviously higher than those of the control group.[Conclusions] The growth of bony callus,the index of the osteoblast,the metrical calcification and the remodeling of the bony callus could all be enhanced by the red rice and therefore the bone union could be accelerated.