Objective:To explore the effect of verapamil on the activition of ?1(Ⅰ) collagen gene promotor induced by TGF ?1. Methods: The recombinant pCOLH 1.5 and pCOLH2.5 of ?1(Ⅰ) collagen gene promotor were transfected to rat mesangial cell and were treated with different concentration of TGF ?1 and verapamil. The activity of CAT were measured by ELISA method. Results:TGF ?1 enhanced the activity of pCOLH1.5 and pCOLH2.5(1.498 and 1.551 fold,respectively) significantly compared to the control; Verapamil inhibited the activity of the 2 recombinants. The activity of CAT stimulated by verapamil + TGF ?1 was remarkably decreased compared with the activity of pCOLH2.5 promotor by TGF ?1. Conclusion:Verapamil can not only inhibit the activity of gene promotor derectly, but also partly block the activity of ?1(Ⅰ) collagen gene promotor induced by TGF ?1.

Objective To investigate the expression of lentivirus-mediated short hairpin RNA (shRNA) targeting collagen typeⅠ (Col Ⅰ) in rat mesangial cells.MethodsThe Col Ⅰ shRNA lentivirus vector was constructed with three-plasmids packaging approach which encoding human/rat homology Col Ⅰ gene.Rat mesangial cells were infected by infection enhancer (control group),the empty lentiviral vector (pSC-GFP group),and lentiviral expression vector (pSC-GFP/ColⅠ group),respectively.Infection efficiency in the three groups was detected with flow cytometry,and the expression of Col Ⅰ mRNA and protein in the three groups was respectively detected by RT-PCR and Western blotting.ResultsRat mesangial cells were efficiently infected in pSC-GFP group and pSC-GFP/Col Ⅰ group.The infection efficiency was 72.42% in pSC-GFP group and 75.42% in pSC-GFP/Col Ⅰ group.RT-PCR showed that the expression of Col Ⅰ mRNA in pSC-GFP/Col Ⅰ group was significantly lower than that in control group and pSC-GFP group (P

Objective To reproduce an animal model simulating acute kidney injury caused high intensity military training in rats by forced exhausting running on the treadmill,that could be used for the investigation of problems arising from military training.Methods 40 male SD rats were randomly divided into three groups:control group(n=8),ordinary exercise group(n=16)and forced exercise group(n=16).The animals in ordinary exercise group were commonly fed.The animals in the other two groups were forced to take on one-time exhausting running exercises on the treadmill by different ways.The rats in forced exercise group were forced to run on the treadmill till exhaustion to reproduce the animal model of overtraining.The indexes of urine,serum and kidney tissues were observed and analyzed shortly after training and 24 hours later.Results After training,the values of urine protein,urinary albumin,urease,serum urea nitrogen,creatinine,angiotensin Ⅱ in serum and kidney tissues of the animals in forced exercise group increased obviously compared with those in control group and ordinary exercise group.Those indexes in ordinary exercise group were higher than in control group(P

Objective To study the effect of simulated intensive military training on the activity of the Na+,K+-ATPase in kidney of rats. Methods ① Twenty four rats were randomly divided into single bout of exhaustive exercise group(B) and control group(A).Rats in group B were forced to run on the treadmill until exhaustion according to the Bedford protocol,and their urine and the activity of Na+,K+-ATPase in kidney were tested immediately(B1,n=16) and 24 hours(B2,n=8) after exhaustion.② Another forty rats were randomly divided into 8-week incremental exercise group(D,n=20) and control group(C,n=20).Rats in group D were forced to run on the treadmill according to the incremental protocol for 8 weeks.The urine and the activity of Na+,K+-ATPase in kidney of group C and D were tested by the end of 4th week(C1 and D1) and by the end of 8th week(C2 and D2). Results ① As compared with group A,the values of urinary protein and urease in group B increased and the activity of Na+,K+-ATPase in kidney decreased obviously(P

Objective To explore the feasibility and value of low dose CT scan for the diagnosis of pulmonary tuberculosis.Methods 150 patients with pulmonary tuberculosis underwent three doses CT scan (standard dose:150 mA;low-dose:15 mA and 30 mA) using GE dual slices helical CT.Besides the different tube current,other scan parameters including tube voltage,scan cycle,pitch,and collimation were the same in three dose groups.The scanned extent was from apexes to bases of lung.Image quality in standard group was compared with that in other two low dose groups and analyzed statistically by three radiologists.Results CT characteristics of pulmonary tuberculosis could be detected efficiently using low dose CT scan(30 mA) program,which was no significant as compared with the CT image using standard CT sose(P＞0.05).Howere,CT scan at 15 mA obviously affected on the diagnosis for both active and inactive pulmonary tuberculosis,the difference was significant (P＜0.05).Conclusion Low dose CT scan can replace totally the standard dose CT scan in diagnosing pulmonary tubercrulosis.

Objective To investigate the dynamic changes and relationship between the serum levels of IL-1 β and sE-selectin in acute pancreatitis (AP) patients, and to study the predicative value for severity of AP.Methods Forty one patients with AP were divided into severe acute pancreatitis (SAP, 19 cases) group and mild acute pancreatitis (MAP, 22 cases) group.Their serum were collected at the 1st, 3rd, 7th, and 14th day after admission and the serum concentrations of IL-1 β and sE-selectin were detected by ELISA.Another 20healthy volunteers were selected as controls.Results The serum concentration of IL-1 β in control group was ( 18.71 ±2.43)ng/L, while they were (61.18 ±7.47)ng/L, (33.03 ±5.85)ng/L, (20.73 ±4.07)ng/L in MAP group at the 1st, 3rd, 7th day;and they were (86.91 ± 13.32) rng/L, (81.35 ± 12.71) ng/L,(64.93 ±5.99)ng/L, (21.40±49.13) ng/L at the 1st, 3rd, 7th and 14th day in SAP group.The serum concentration of IL - 1β of patients with SAP were markedly higher than those with MAP and normal controls on the 1st, 3rd, 7th day (P ＜0.05) and they decreased almost to normal on the 14th day.The serum concentration of sE-selectin was ( 10.69 ± 2.51 ) ng/ml, while they were ( 41.60 ± 6.85 ) ng/ml, ( 14.90 ±3.51)ng/ml, (9.85 ±2.88)ng/ml in MAP group at the 1st, 3rd, 7th day;and they were (84.73 ±15.37)ng/ml, ( 95.65 ± 13.06 ) ng/ml, ( 39.41 ± 3.73 ) ng/ml, ( 12.25 ± 2.29 ) ng/mlon the 1st, 3rd, 7 th and 14th day.The serum concentration of sE-selectin of patients with SAP were significantly higher than those with MAP and normal controls on the 1st, 3rd, 7th day (P ＜0.05 ) and there was no significant difference between SAP and control group on the 14th day.There was a positive correlation between the serum level of IL-1 β and sE-selectin in AP on the 1 st day after admission ( r = 0.851, P ＜ 0.01 ).Conclusions The serum concentrations of IL-1 β and sE-selectin are useful for AP severity predication.

Humans ; Hydrocarbons, Brominated ; analysis ; metabolism

BACKGROUND:It remains hard to judge characterization of lesion tissues using two-dimensional sectional image at present. Medical three-dimensional reconstruction technique could obviously improve work efficiency and accuracy of medical working staff on disease diagnosis.
OBJECTIVE:To develop a three-dimensional medical visualization system can rebuild three-dimensional model of corresponding parts by reading DICOM data, and observe the appearance of affected hip joint using reconstructed models.
METHODS:A three-dimensional (3D) medical visualization system was developed using the MFC in a PC with Windows XP operating system, development environment VC++6.0, VTK 5.6 instal ation, and necessary settings were made. Precise procedures are as fol ows: ① create a drawing object; ② create a drawing window, and draw objects was added in draw window; ③ read the CT image sequences, and set the path of the read image sequence; ④ isosurface (generate triangular facets) were extracted using MC algorithm, depending on the gray, skin and bones. Data of the input image sequence were set;gray value of tissue contour was set; ⑤the establishment of a V-belt objects and data mapping object; ⑥ graphics rendering, to receive the properties of the geometric data, and a different colors and transparency of bones and skin were set; ⑦ the viewing position was set to observe the position of the object and focus; ⑧ to create a human-computer interaction function.
RESULTS AND CONCLUSION:VC++6.0 and VTK could meet the needs of medical 3D visualization systems development. Developed 3D visualization system software could perform 3D reconstruction through reading CT images of DICOM format files. Thus, the relevant parts of the reconstructed 3D model could be observed by rotating, zooming, and panning so as to visual y observe the bone structure of hip joint, fractured appearance and type. It provided a reference for relevant therapy and operation.

BACKGROUND:It is difficult to adjust the anteversion angle of cementless hip joint. Some areas rely on the intraoperative three-dimensional navigation technique to ensure the accuracy of the anteversion angle, but its high cost limits the promotion prospects. OBJECTIVE:To design a kind of artificial femur head, which has special functions to freely adjust the anteversion angle of artificial femur head during operation and lock prosthesis handle to prevent femur prosthesis dislocation and looseness, and to intuitively display the special design concept of this kind of artificial femur head through the three-dimensional dynamic image aided by computer. METHODS:According to the design idea, AutoCAD software was used to get the design drawings for artificial femur head. The three-dimensional modeling was performed by 3DMax software in order to observe the form and degrees of verisimilitude of model. RESULTS AND CONCLUSION:The AutoCAD software was used to draw out the design drawings of artificial femur head according to the design requirements and design idea:the prosthesis handle had three lock hole channel and its basal part was for scale, and rotating the scale could change the anteversion angle of prosthesis neck;the shape of the prosthesis neck was dentate cylindrical. The 3DMax software was used to build the three-dimensional model of the artificial femur head, and the design, form and degrees of verisimilitude of the model comply with the design requirements. The whole structure of the three-dimensional model of artificial femur head is clear and the design is reasonable, which can provide a theoretical reference for further design of artificial femur head.

Objective To investigate the effect of bortezomib on proliferation and apoptosis of pancreatic cancer cell lines BxPC3,SW1990 and explore possible mechanisms of bortezomib's killing effect on cancer cells.Methods BxPC3,SW1990 cells were treated by using 1,10,50,100,500 nmol/L and 1,10 μmol/L of bortezomib,and cells without bortezomib treatment were considered as control group.The cell proliferation was determined by MTF assay,and apoptosis was determined by flow cytometry.Bak,Bax,Bcl2,Bcl-xl,survivin mRNA expressions were measured by RT-PCR,and Western blot was applied to determine the expressions of pro-caspase-3,cleaved-caspase-3,Bax,Bcl-2,surviving protein.Results When bortezomib concentration was higher than 50 nmol/L,it inhibited the proliferation of two cell lines in a dose and time-dependent manner.And with the same treatment the rate of proliferation inhibition of BxPC3 cells by bortezomib was greater than that of SW1990 cells,and the difference between the two cell lines was statistically significant (P ＜0.05).Apoptosis rates in the groups of BxPC-3 and SW1990 cells treated by 100 nmol/L bortezomib were (22.56 ± 4.23) ％ and (12.71 ± 2.23) ％,which were significantly higher than those in control group (2.15 ± 0.47) ％ and (2.32 ± 0.54) ％,P ＜ 0.05).In addition,apoptosis rate of BxPC3 cells was significantly higher than that of SW1990 cells (P＜0.05).Bak mRNA expression of BxPC3 and SW1990 cells after 100 nmol/L bortezomib treatment were not significantly changed,but the expression of Bax mRNA and protein was significantly increased (P ＜0.05).Bcl-2 mRNA and protein,as well as Bcl-xl mRNA expressions was significantly decreased (P ＜0.05).The expression of survivin mRNA and protein in BxPC3 cells were decreased,but were increased in SW1990 cells(P ＜0.05).The expression of pro-caspase-3 protein in the two cell lines was decreased,while the expression of cleaved-caspase-3 protein was increased (P ＜0.05).Conclusions Bortezomib can inhibit the proliferation of pancreatic cancer cell Iines BxPC-3 and SW1990 and induce apoptosis,and the effect on BxPC3 cells is more than that on SW1990 cells.The mechanism may depend on activation of the mitochondrial pathway of apoptosis,and be related to survivin-involved drug-resistance.