1.The nursing effect of health education on patients with peptic ulcer
Xisui CHEN ; Huijun LI ; Yongzhong SU ; Yinghua LI ; Chunhua PENG
Chinese Journal of Primary Medicine and Pharmacy 2008;15(6):927-928
Objective To explore the nursing effect of health education on patients with peptic ulcer. Meth-otis 108 patients with peptic ulcer were randomly divided into 2 groups:intervention group and control group. Each consisted of 54 samples and was given the same medication. Besides, the control group was additionally cared with routine heath education while the intervention group was given the comprehensive health education. Results Com-prehensive health education reduced the course of hospital,alleviated symptoms of digestion and improved the peptic ulcer,while the routine heath education made no significant progress in either aspect. Differenees of knowledge as well as attitude score was of statistical significance(P≤0.05) between before and after heath education were given except the score of behavior ( P > 0.05 ). Conclusion Comprehensive health education could significantly improve curative effect for the treatment of peptic ulcer.
2.Dual kidney transplantation from brain death adult donors: two case reports and literature review
Fenghua PENG ; Longkai PENG ; Chen GAO ; Jingliang CHEN ; Yong GUO ; Chunhua FANG ; Manhua NIE
Journal of Chinese Physician 2017;19(1):22-24,28
Objective To explore the use of marginal kidneys from DBD (donation after brain death) donors and the indication for adult dual kidney transplantation.Methods Two pairs of graft kidneys were procured from two marginal adult donors.Dual kidney transplants were performed in two recipients.In each recipient,two kidneys were implanted in unilateral site of right lower quadrant and placed extraperitoneally,two separate extravesical ureterneocysto-anastomoses were performed.Results Delayed graft function (DGF) combined with acute rejection occurred in two cases,and all two cases recovered after treatments.Lymphocele and hematoma occurred in one case.No graft embolism and no urinary leak happened.Conclusions Adult dual kidney transplant offers an important use of kidneys from marginal donors to increase the number of organs available for transplantation.
3.Preparation of a fusion protein with pneumococcal surface protein A of two different clades of familyⅠand analysis of immunogenicity of the recombinant protein
Haiying LIN ; Yonghui PENG ; Shuangling ZHANG ; Chunhua LUO ; Meiyun ZHENG ; Wei LYU ; Duqing ZHAN
Chinese Journal of Microbiology and Immunology 2015;(5):377-381
Objective To construct a recombinant fusion protein with pneumococcal surface pro-tein A (PspA) of Stretococcus pneumonia (SPN) familyⅠclade 1 and 2, and to analyze the immunogenici-ty of the fusion protein.Methods The gene fragments encoding theα-helix of PspA of the two clades were amplified by PCR and then inserted into the expression vector pET-27b(+) to construct the recombinant ex-pression plasmid.The transformed Escherichia coli BL21 strains carrying expression plasmid were induced by IPTG to express the recombinant protein.The titers and affinity of antibodies against PspA protein were measured by ELISA.An opsonophagocytic assay and an animal experiment were performed to evaluate the immunogenicity of the recombinant protein.Results Double enzyme cutting and gene sequencing confirmed the two purpose gene fragments were correctly expressed in the expression vector pET-27b(+).The titers of anti-PspA antibody in the serum of Kunming ( KM) mice immunized with the fusion protein were 1 ×104 . The affinity of anti-PspA antibody reached to 2×105 .The rates of recombinant PspA6B-PspA05 protein me-diated phagocytosis for SPN6B, SPN05 and SPN01 strains were 20%, 15% and 8.8%, respectively.No SPN23F strain was engulfed by macrophages upon the stimulation with PspA6B-PspA05 protein.The survival rates of mice injected with SPN05, SPN6B, SPN01 and SPN23F strains were respectively 75%, 92%, 75%and 33%upon the immunization of PspA6B-PspA05 protein.Conclusion The recombinant fusion protein PspA6B-PspA05, constructed with the PspA proteins of Stretococcus pneumonia familyⅠclade 1 and 2, was successfully expressed in the E.coli prokaryotic system with the advantage of high immunogenicity.High ti-ters of anti-PspA antibodies with high specificity were induced in KM mice upon the stimulation with Ps-pA6B-PspA05 protein.Moreover, a cross-protective immunity was induced in KM mice upon the immuniza-tion with PspA6B-PspA05 protein.
4.PPARγagonist inhibits high glucose-induced production of reactive oxy-gen species by UCP2 up-regulation
Peijian WANG ; Qiulin WANG ; Zhen YANG ; Fang WANG ; Chunhua PU ; Wenzhang LI ; Dengpan LIANG ; Peng ZHOU
Chinese Journal of Pathophysiology 2015;(1):49-53
AIM:To explore the effects of PPARγon the elevated level of reactive oxygen species ( ROS) in-duced by high glucose and its mechanism .METHODS:Human umbilical vein endothelial cells ( HUVECs) were cultured with DMEM containing high glucose (33 mmol/L D-glucose), and DMEM containing lower glucose (5.5 mmol/L D-glu-cose) was used as control .Superoxide anion and nitric oxide fluorescence probes were used to observe the effects of PPAR γagonist on ROS and NO productions in the HUVECs .The uncoupling protein 2 (UCP2) protein level in the HUVECs was detected by Western blotting .RESULTS:PPARγagonist pioglitazone inhibited the ROS generation and prevented the de-crease in NO level under high glucose condition , and these effects were reversed by pretreatment with PPARγantagonist GW9662.The results of Western blotting indicated that PPARγagonist pioglitazone up-regulated the UCP2 expression un-der high glucose condition , and this effect was also blocked by GW 9662.Inhibition of UCP2 by genipin attenuated the effect of pioglotazone on the ROS production .CONCLUSION: Activation of PPARγinhibits ROS generation under high glucose condition , and this effect may mediate by up-regulation of UCP2.
5.Analysis of Mind-control and Qi-regulation:Tracing the Arrival of Qi Recorded by Nei Jing and Nan Jing
Jie HAO ; Jiang ZHU ; Peng ZHANG ; Chunhua LI ; Chi LIN ; Nijuan HU ; Siyuan XIN ; Jing LI
Shanghai Journal of Acupuncture and Moxibustion 2014;(10):879-882
Objective To discuss the relationships between qi-arrival and mind-control, qi-arrival and qi-regulation, and to define qi-arrival by tracing the relevant records in Nei Jing (Classic of Internal Medicine) and Nan Jing (Classic of Difficult Issues). Method Thirty-seven relevant ancient books of the two classics were reviewed and the notes by WANG Bing, MA Shi, and YANG Shang-shan were referred. Result Mind-control requires the spirits of the practitioner, patient and environment, as well as observing and waiting for the arrival of qi. Regarding qi-regulation, theories involve that qi-arrival achieves same reinforcing and reducing effect as qi-regulation, qi-regulation is elucidated from the correct location of acupoint, proper needling depth and direction, and to regulate qi by observing the arrival of qi. Besides, the changes of internal and external environments also influence qi-arrival via qi-regulation and mind-control. Conclusion Qi-arrival is closely related to mind-control and qi-regulation in obtaining, differentiation, and adjustment. Mind-control and qi-regulation assist each other through the whole acupuncture process, which is the core of qi-arrival.
6.Relationship between plasminogen activator inhibitor-1 gene polymorphism and gastrointestinal bleeding in ;Henoch-Sch?nlein purpura
Baoxiang WANG ; Hong MEI ; Hanming PENG ; Yuan GAO ; Chunhua YU ; Yan DING
Journal of Clinical Pediatrics 2016;34(3):197-200
Objective To investigate the relationship of single nucleotide polymorphism (4G/5G) in the promoter of plasminogen activator inhibitor-1 (PAI-1) and plasma PAI-1 level with gastrointestinal bleeding in Henoch-Sch?nlein purpura (HSP). Methods A total of 524 children with HSP in acute phase were recruited, and divided into gastrointestinal bleeding group (bleeding group, n?=?186) and non-gastrointestinal bleeding group (control group, n?=?338). The genotype frequency of 4G/5G polymorphism, the plasma PAI-1 level, and other parameters related to coagulation and ifbrinolysis were measured and compared between two groups. Results The levels of platelet count (PLT), platelet distribution width (PDW), serum D dimer (DD), serum PAI-1 were signiifcantly higher in the bleeding group than those in the control group, and the levels of mean platelet volume (MPV) and plasma ifbronectin protein of ifbrinogen (FIB) were signiifcantly lower in the bleeding group than those in the control group (P?0.05). The genotype frequency of 4G/4G was signiifcantly higher in the bleeding group than that in the control group (P?=?0.044). The plasma PAI-1 level and DD level was high in 4G/4G genotype. Conclusions The gene polymorphism of PAI-1 4G/5G may affect the pathological process of gastrointestinal bleeding in HSP by inlfuencing the expression of PAI-1 and other factors related to coagulation and ifbrinolysis systems.
7.Drug release of Compound Salvia Multi-component released Double-layer Tablet
Changchun PENG ; Qin ZHENG ; Pengyi HU ; Junjie PAN ; Chunhua LAI ; Ming YANG
Chinese Traditional Patent Medicine 2010;(1):39-43
AIM: To establish the drug release method of Compound Salvia Multi-component released Doublelayer Tablet and investigate its in vitro drug release behavior and the influencing factors of the drug release rate.METHODS: The method of evaluating in vitro release rate of double-layer tablet was established with salvianolic acid B and gindenoside Rg_1 as index,The influencing factors of the release of rapid release layer and sustained release layer of double-layer tablet and their in vitro drug release behavior were studied.RESULTS: The rapid release layer showed the quick releasing effect; The drug release curve of sustained release layer accorded with Ritger-Peppas equation; artificial gastric juice had significant effect on the release curve of gindenoside Rg_1 which in double-layer tablets,but had no significant effect on the release curve of salvianolic acid B.CONCLUSION: The evaluation of in vitro release rate of Compound Salvia Multi-component released Double-layer Tablet shows good properties of fast and sustained release and clinic application is achieved.
8.Design and Application of Multimedia Courseware in Inorganic Chemistry
Yian PENG ; Limin LIU ; Chunhua LEI ; Xiaomei CAO ; Qihua LI ; Xiaojuan ZHU
Chinese Journal of Medical Education Research 2003;0(03):-
By taking the 《Inorganic Chemistry》of the national standard textbook for the pharmaceutical speciality as a chief source (chief editonHou Xinchu), adopting the softwares of powerpoint, ISIS/ Draw, formula edit tool...etc., and combining the download from the internet, video and refering to the related coursewares in other colleges ...etc., we have and manufactured the multimedia courseware of 《Inorganic Chemistry 》,composed perfectly of various means such as writing, picture, showbiz, animation, voice...etc. This courseware has already been applied in 2001 class,2002 class of the pharmaceutical specialty in our college and has achieved a good result.
9.Test-retest Reliability of Tetrax Posturographic Balance Assessment System in Elderly
Li ZHANG ; Chunhua LI ; Changshui WENG ; Qiuhua WANG ; Wei CHEN ; Nan PENG
Chinese Journal of Rehabilitation Theory and Practice 2011;17(7):637-639
Objective To assess the test-retest reliability of Tetrax posturographic balance assessment system in determining the balance function in elderly individuals. Methods 22 elderly subjects (66~84 years old) were measured with the stability index (ST), the spectrum analysis of postural sway and the weight distribution harmony between two foots or heels and toe parts in standing on solid surface with eyes open using Tetrax balance system. All tests were repeated 1 week after the initial test and each subject underwent the test under the same instructions and conditions. The intraclass correlation coefficients (ICC) were used to determine the test-retest reliability. Results ICC of all balance functional parameters ranged from 0.797~0.882 (P<0.01). Conclusion The posturographic balance assessment system has good test-retest reliability for determining the balance function in elderly people.
10.Effect of High-voltage Electrostatic Therapy on Sleep Disorder in Older Adults
Li ZHANG ; Nan PENG ; Wei CHEN ; Qiuhua WANG ; Xiaoying LI ; Chunhua LI
Chinese Journal of Rehabilitation Theory and Practice 2012;18(3):286-288
Objective To evaluate the effect of high-voltage electrostatic therapy on sleep disorder in the elderly. Methods 31 elderlycases (66~92 years ) with sleep disorder were treated with high-voltage electrostatic therapy for 10~15 d. They were assessed with PittsburghSleep Quality Index (PSQI) before and after the intervention. Results The global score and all the subscale scores of PSQI decreased(P<0.01) after intervention. Conclusion The high-voltage electrostatic therapy is effective on sleep quality.