By comparing routine FBP with algebraic iterative reconstruction, this study exploit the effect of the two SPECT reconstruction techniques on the ratio of Target/Background (T/B) and image noise. The result of research on model and clinical cases showed that the technique of OSEM could increase T/B ratio by 1.5% (heart), 3.3% (bone), 1.4% (brain). No obvious difference in image noise existed between OSEM and FBP. Compared with FBP, OSEM could improve T/B value in bone focus more significantly. Under the same injection dosage, OSEM could increase the speed of image collection by more than one time without changes in image quality and T/B ratio. The result suggested that function and clinical application of SPECT would significantly improved by substituting routine FBP with OSEM.
Algorithms ; Humans ; Image Processing, Computer-Assisted ; methods ; Tomography, Emission-Computed, Single-Photon ; methods

Objective　To observe the characteristics and rules of craniocerebral injury resulting from a high explosive shell to provide the bases for treating explosive injury. Methods　A total of 36 sheep were distributed at the distance 6 to 48 m away from the explosive center and the shell was exploded electrically at 7 m above the earth. At the same time, the velocity of fragments and shock wave pressure were determined. Gross and pathological observations were performed after injury. Results　Among all sheep with fragment injury, craniocerebral injury was 32%. Their immediate death rate was 75% and all died 6 h later. The incidence rates of penetrating wound and blind wound were 75% and 25% respectively. Pollution of wound track was heavy. The percentage of head lost was 50% in sheep and 50% of injured animal suffered from comminuted fracture of skull base. Bleeding was found extensively on the surface of the cerebrum, even medulla oblongata was involved. Hemorrhage, edema, rupture of small blood vessels and degeneration of neuron were found at the regions 4 cm away from the wound tract with light microscopy. Combined blast injury was found and occurred most often in the abdomen and limbs, both accounting for 62.5%, and combined thoracic injury was the third, up to 50%. All the animals of craniocerebral injury combined with lung blast injury. Conclusion　High explosive shells destroy cranium badly and extensively. Many skulls are lost and the cranial base is readily fractured. The wound track is heavily polluted. Combined injury is more often occurred.

A century ago, more exactly 102 years ago, there was a devastating pandemic of influenza in 1918 and thereafter, periodic recurrences of pandemic events have been reported in the human population. Unfortunately, whenever it happened, the outcome was concomitant with over millions of death tolls due to considerably higher case fatality rates, compared to other infectious diseases at that time. In this regard, pandemics, which continued at irregular time intervals, give a great significance to global public health responses. However, it is far from feasibility to predict when a next pandemic will begin and how much disease burden will be despite our efforts to utilize all kinds of available scientific information and knowledge. The one clear thing is that approximately 70% of the causative agents of emerging and/or re-emerging diseases including COVID-19 (coronavirus disease 2019), which has been started from Wuhan province, China in December 2019 and has resulted in more than 4 million human cases within a few months, are viruses. Therefore, it is very important to secure fast and accurate identification methods of a causative pathogen in order to provide scientific clues and to prepare in advance for the abrupt occurrence of unknown viral diseases in a timely manner. In this review, the current status and future perspectives of the molecular technology for identification of viral pathogens such as SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) with regard to rapid public health responses in the early stage of infectious diseases including COVID-19, will be discussed.

OBJECTIVETo evaluate clinical factors affecting Gleason score upgrade in patients receiving radical prostatectomy (RP).

METHODSA total of 322 patients with prostate cancer who received RP from January 2012 to December 2013 at Department of Urology at Fudan University Shanghai Cancer Center were included, and their data of age, body mass index (BMI), prostate-specific antigen (PSA), prostate volume, percentage core, clinical staging, pathological characteristics, biopsy Gleason score and RP Gleason score were analyzed. Differences in categorical variables and continuous variables were compared using χ² tests and Student's t-test, respectively. Unconditional multiple logistic regression was used to estimate OR and 95% CI of the association of Gleason score upgrade with clinical factors.

RESULTSGleason score upgrade occurred in 107 of 322 (33.3%) patients. There was no difference in age, BMI and clinical staging between the two groups. Compared with patients without Gleason score upgrade, higher levels of PSA (χ² =6.740, P=0.034), smaller prostate volume (t=3.481, P=0.002) and elevated percentage core (t=-2.097, P=0.037) were observed in patients with Gleason score upgrade. In addition, lymph node metastasis (χ² =4.193, P=0.041) and extracapsular extension (χ² =4.747, P=0.029) were more common in patients with Gleason score upgrade. After adjusting for potential confounders, PSA levels (OR=2.451, 95% CI: 1.290-4.660), prostate volume (OR=0.982, 95% CI: 0.969-0.995) and percentage core (OR=2.756, 95% CI: 1.033-7.357) were independent predictors for Gleason score upgrade.

CONCLUSIONGleason score upgrade happens at a relatively high rate. PSA levels, prostate volume and percentage core are important factors affecting Gleason score upgrade.

Biopsy ; Body Mass Index ; China ; Humans ; Logistic Models ; Male ; Multivariate Analysis ; Neoplasm Grading ; Prostate-Specific Antigen ; blood ; Prostatectomy ; Prostatic Neoplasms ; diagnosis ; surgery