Objective To comparatively analyze the advantages and disadvantages between a double immune-diffusion assay and a rate nephelometer analysis for the detection of antigen activity of pneu -mococcal capsular polysaccharides .Methods The antigen activity of pneumococcal capsular polysaccharides of serotypes 1,6B,9V,10A,14 and 19A from four manufacturers and ATCC were analyzed by a double im-mune-diffusion assay and a rate nephelometer analysis , respectively .The effects of antiserum samples and gain values on the rate response value were evaluated .Results The sample 4 of type 9V showed no antigeni-city with a rate response value similar to that of negative control as indicated by both tests .However ,the pre-cipitation lines and the rate response values presented by other polysaccharide samples differed in a wide range.Results of the rate nephelometer analysis were not affected by the anti -serum samples from different sources and the gain values .Conclusion The rate nephelometer analysis could quantitatively analyze the antigen activity of pneumococcal capsular polysaccharides .

OBJECTIVE:To study the therapeutic effect of granisetron on postoperative nausea and vomiting(PONV) under patient controlled analgesia(PCA) and to observe the influence of infusion rate of granisetron on blood circulation during operation METHODS: 90 selective surgical patients were divided into 3 groups A group(control):ondonsetron 8mg;B group:tropiestron 3mg; C group:granisetron 3mg The solutions of granisetron hydrochloride were infusied at 20,30,40,50 and 60 min before the end of operation The change of the circulation condition and the status of nausea and vomiting were observed at infusion period and 4,8,12,24hs,2d and 3d postoperatively RESULTS:Under expansion of blood volume,if infusion duration was longer than 20 min for granisetron,the circulation condition(MAP,HR) were not affected during infusion period and no headache was found There were significant differences in PONV between group A and group B(P

The effect of AMP-activated protein kinase (AMPK) on KiSS-1 mRNA levels was detected by realtime PCR in the hypothalamic GT1-7 neurons. The promoter activity of KiSS-1 gene was detected by DualLuciferase Reporter Assay System.The effects of AMPK on the protein expression and subcellular distribution of SP1 were determined by Western blot.The results showed that AMPK reduced the mRNA expression and promoter activity of KiSS-1 gene while SP1 increased the promoter activity of KiSS-1 gene. Besides,AMPK alse decreased the translocation of SP1.These results suggest that AMPK may inhibit the expression of KiSS-1 gene by decreasing the translocation of SP1 from cytoplasm to nucleus in the hypothalamus GT1-7 neurons.

Objective To establish a standardized quantitative enzyme-linked immunosorbent as-say ( ELISA) recommended by WHO for the detection of human IgG antibodies specific for Streptococcus pneumoniae capsular polysaccharides ( Pn PS ELISA ) .Methods According to the WHO recommended standard Pn PS ELISA protocol , capsular polysaccharide concentrations of 13 serotypes (1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F, 23F) of Streptococcus pneumonia for coating were optimized;the ELISA plates and AP conjugated goat anti-human IgG antibody for the detection were experimentally selected .Using the established assay parameters assured by testing quality control standards ( QCs) provided by WHO refer-ence laboratory , a panel of 16 LIBP ( Lanzhou Institute of Biological Products Co .Ltd.) QCs were measured for comparison analysis between WHO reference laboratory and LIBP laboratory .In the meantime , the range of IgG concentrations of an internal QC panel 907 for 13 serotypes of pneumococcal capsular polysaccharide were established for routine QC work in LIBP laboratory , and inter-assay precision within LIBP laboratory was assessed as well .Results The standardized Pn PS ELISA assay established in LIBP laboratory met the criteria required by WHO .There was a high correlation between the data collected by WHO reference labora -tory and LIBP laboratory (slope=0.94, coefficient of correlation r=0.97, P<0.05).Eighty one percent of IgG concentrations measured by LIBP laboratory were within the range of ±40%of those measured by WHO reference laboratory , which met the criteria of 75%data falling within the ambit of ±40%of assigned values commonly used for comparison .The ranges of IgG concentration in LIBP QC 907 for 13 serotypes had been established.The inter-assay precision in LIBP laboratory was high with coefficiency of variation ( CV) less than 30%.Conclusion LIBP laboratory has successfully established the standardized ELISA recommended by WHO for quantitative detection of human IgG antibodies against pneumococcal capsular polysaccharides (Pn PS ELISA).The range of IgG concentrations in LIBP QC 907 for 13 serotypes of pneumococcal capsular polysaccharide are also established for routine quality-control practice .

Objective:To explore the predictive value of deep learning based on three dimensional deep residual network(3D Res-Unet)model for the dose accuracy of postoperative volume modulated arc therapy(VMAT)plan of endometrial carcinoma.Methods:A retrospective collection of 154 VMAT radiotherapy plans for endometrial carcinoma from The First People's Hospital of Neijiang was conducted.The data set was divided into one training set with 108 cases,one validation set with 15 cases and one test set with 31 cases as the ratio of 7:1:2 through randomly sampling.The approved dose of clinical application was used as"gold standard"to compare the difference between predictive radiotherapy dose of 3D Res-UNet and clinically radiotherapy dose.Results:There were statistical differences in the conformity index(CI)of target area and average dose(Dmean)between deep learning and clinical gold standard(t=-3.115,-0.124,P<0.05),and the difference of bladder V40 of organ at risk(OAR)between them was significant(t=0.510,P<0.05),and the difference of rectum V50 between them was significant(t=-2.121,P<0.05).The predictive dose of the left femoral head V30 was significantly lower than that of clinical dose(t=0.415,P<0.05).The predictive dose of the right femoral head V30 was significantly lower than that of clinical dose(t=-3.102,P<0.05).The predictive dose of pelvic Dmean was significantly higher than that of clinical dose(t=1.224,P<0.05).The predictive dose of small intestine V40 was significantly higher than that of clinical dose(t=0.461,P<0.05).There were no statistically significant difference in other indicators(P>0.05).The difference plot of dose showed that there was few difference between predictive results and clinical results,and the dose volume histogram of prediction basically coincided with that of clinical application.Conclusion:The 3DRes-UNet model can effectively predict the three-dimensionally spatial dose of VMAT plan after surgery for endometrial carcinoma,which can guide clinical radiotherapy work.

Arabidopsis BOTRYTIS-INDUCED KINASE1 (BIK1) is a receptor-like cytoplasmic kinase acting early in multiple signaling pathways important for plant growth and innate immunity. It is known to form a signaling complex with a cell-surface receptor FLS2 and a co-receptor kinase BAK1 to transduce signals upon perception of pathogen-associated molecular patterns (PAMPs). Although site-specific phosphorylation is speculated to mediate the activation and function of BIK1, few studies have been devoted to complete profiling of BIK1 phosphorylation residues. Here, we identified nineteen in vitro autophosphorylation sites of BIK1 including three phosphotyrosine sites, thereby proving BIK1 is a dual-specificity kinase for the first time. The kinase activity of BIK1 substitution mutants were explicitly assessed using quantitative mass spectrometry (MS). Thr-237, Thr-242 and Tyr-250 were found to most significantly affect BIK1 activity in autophosphorylation and phosphorylation of BAK1 in vitro. A structural model of BIK1 was built to further illustrate the molecular functions of specific phosphorylation residues. We also mapped new sites of FLS2 phosphorylation by BIK1, which are different from those by BAK1. These in vitro results could provide new hypotheses for more in-depth in vivo studies leading to deeper understanding of how phosphorylation contributes to BIK1 activation and mediates downstream signaling specificity.
Amino Acids ; chemistry ; Arabidopsis ; enzymology ; Arabidopsis Proteins ; chemistry ; genetics ; isolation & purification ; Gene Expression Regulation, Plant ; Immunity, Innate ; Mutation ; Phosphorylation ; Protein-Serine-Threonine Kinases ; chemistry ; genetics ; isolation & purification ; Signal Transduction ; Threonine ; genetics